Optimization of Ultrasound Extraction of Cactus Pear (Opuntia ficus indica) Seed Oil Based on Antioxidant Activity and Evaluation of Its Antimicrobial Activity

Table 4

Antimicrobial activity of cactus pear seed oil extracted by different methods.

	Diameter of inhibition zone (mm)
	Oil	(+) control	(−) control
Staphylococcus aureus (Gram-positive)
Ultrasound	9.17 ± 0.29	18.89 ± 1.54	ND
Soxhlet	9.50 ± 0.87	18.78 ± 1.64	ND
Maceration	9.78 ± 0.69	18.11 ± 1.17	ND
Escherichia coli (Gram-negative)
Ultrasound	7.78 ± 0.19	15.56 ± 2.12	ND
Soxhlet	7.56 ± 0.19	15.33 ± 2.65	ND
Maceration	7.56 ± 0.38	14.00 ± 2.33	ND
Pseudomonas aeruginosa (Gram-negative)
Ultrasound	ND	12.56 ± 0.38	ND
Soxhlet	ND	12.22 ± 0.38	ND
Maceration	ND	12.78 ± 0.38	ND

Seed oil: 66.67 µL; (+) control: ampicillin (disc 10 µg; Staphylococcus aureus and Escherichia coli) and streptomycin (disc 10 µg; Pseudomonas aeruginosa); (−) control: hexane; ND: not detected; ±: standard deviation; Same superscripts indicates that there is no significant difference (p > 0.05).