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Journal of Food Quality
Volume 2017, Article ID 9718451, 6 pages
https://doi.org/10.1155/2017/9718451
Research Article

Multiscreening LC-MS/MS Designed for Ten Pesticide and Six Antimicrobial Residues in Eggs

1Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista (UNESP), Distrito de Rubião Júnior, s/n, 18618-970 Botucatu, SP, Brazil
2Department of Veterinary Medicine, Federal University of Parana, 80035-050 Curitiba, PR, Brazil
3Center for Advanced Research in Matology (CARM), Chromatography Laboratory, Faculty of Agricultural Sciences, Paulista State University, 18618-970 Botucatu, SP, Brazil

Correspondence should be addressed to Julia Arantes Galvão; moc.liamg@oavlagsetnaraailuj

Received 28 July 2016; Accepted 6 December 2016; Published 22 January 2017

Academic Editor: Ángel A. Carbonell-Barrachina

Copyright © 2017 Julia Arantes Galvão et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A multiscreening method was experimentally validated for pesticide and antimicrobial residues in spiked eggs at levels of 5, 50, and 500 μg L−1. The residue extraction was performed with acetonitrile and 1% formic acid. The extracted material was stored at −70°C until detection and quantitation within 24-hour period by LC-MS/MS. Pesticide and antimicrobial residue recovery performed adequate standard deviation and the results were from 94.10% (ampa) to 9.20% (carbaryl) for pesticides and from 95.70% (enrofloxacin) to 46.20% (sulfathiazole) for antimicrobial. The method was highly accurate, considering the linearity for all pesticide and antimicrobial residues (), with quantitation limits ranging from 0.78 to 6.25 μg L−1 for pesticides and 0.78 to 3.13 μg L−1 for antibiotics. A highly correlation coefficient () was found, except for carbaryl. Although time extraction and carbaryl recovery remain to be improved, the method has shown a rapid pesticide and antimicrobial residue detection.