Investigating Enzyme Activity of Immobilized Candida rugosa Lipase

Table 2

Variation in the Michaelis–Menten constants.

Enzyme	(µM/min)	(µM)	(min^−1)
Free enzyme	0.41	0.97	0.43a
Celite-acetone	0.50	0.87	0.56b
Sephadex-acetone	0.42	0.92	0.43a
Chitosan-acetone	0.25	1.19	0.21
Celite-alcohol	0.46	0.89	0.52c
Sephadex-alcohol	0.41	1.10	0.37
Chitosan-alcohol	0.23	1.25	0.19