Impact of Diets Containing Plant Raw Materials as Fish Meal and Fish Oil Replacement on Rainbow Trout (Oncorhynchus mykiss), Gilthead Sea Bream (Sparus aurata), and Common Carp (Cyprinus carpio) Freshness

Table 2

Formulations and proximate compositions of the gilthead sea bream experimental diets.

Ingredients (g kg^−1 feed)	D1	D2	D3	D4
Fish meal	230	30	30	30
Fish hydrolysate (CPSP)	20	20	20	20
Soya protein	160	250	250	250
Corn gluten	150	250	250	250
Wheat gluten	40.0	73.0	73.0	73.0
Rapeseed cake	120	97.0	99.0	99.0
Wheat	110.8	68.0	66.4	62.4
Fish oil	156	65.6	25.0	25.0
Rapeseed oil	0	44.0	65.0	65.0
Palm olein	0	44.0	65.0	65.0
Monocalcium phosphate	3.03	20.97	20.97	20.97
Histidine	1.36	1.36	1.36	1.36
Mineral-vitamin mi	5.00	5.00	5.00	5.00
Cholesterol	1.13	1.13	1.13	1.13
Amino acid and micronutrient mi	2.00	29.2	27.4	27.4
Antioxidants	0.45	0.45	0.45	0.45
BP-70	0	0	0	4.00
Proximate composition
DM (g kg^−1)	916.5	917.9	918.0	923.4
Protein (g kg^−1)	454.8	467.3	461.2	460.3
Fat (g kg^−1)	198.0	195.6	201.3	194.0
Energy (kJ g^−1 DM)	22.00	22.26	22.25	22.19
Ash (% DM)	66.9	60.6	60.6	64.6
Fatty acid composition; (g kg^−1 total FA)
16:0	166	135	117	119
18:2ω6	69.2	178	198	198
18:3ω3	16.8	37.9	56.2	57.7
20:4ω6	8.40	4.10	1.90	1.80
20:5ω3	131	63.2	29.6	28.9
22:6ω3	91.6	43.9	21.1	20.7
Total SFA	278	246	231	234
Total MUFA	253	345	411	401
Total n-6	224	292	303	311
Total n-3	245	117	551	540

The following is supplied (g kg⁻¹ mix, except as noted): calcium 689, sodium 108, iron 3, manganese 1, zinc 1, cobalt 2 mg, iodine 2 mg, selenium 20 mg, molybdenum 32 mg, retinyl acetate 1, DL-cholecalciferol 2.6, DL-α-tocopheryl acetate 28, menadione sodium bisulphite 2, ascorbic acid 16, thiamin 0.6, riboflavin 1.7, pyridoxine 1.2, vitamin B12 50 mg, nicotinic acid 5, pantothenic acid 3.6, folic acid 0.6, and biotin 50 mg. Containing methionine, lysine, choline, and lecithin.