Saponin Bitterness Reduction of Carica papaya Leaf Extracts through Adsorption of Weakly Basic Ion Exchange Resins

Figure 8

HPLC chromatogram profiles of C. papaya leaves extract (a) standard (escin); (b) fresh (untreated); (c–h) samples treated with ion exchange resins. Red circle in (b–h) shows the retention time of the saponin compound. (a) Peak of standard (control: escin) at concentration of 100 mg/l using 210 nm detection wavelength. (b) Peak of fresh sample (untreated) using 210 nm detection wavelength. (c) No peak detected at the expected retention time of samples treated with Diaion WA21 resin at 5% (w/v) adsorbent dose using 210 nm detection wavelength. (d) No peak detected at the expected retention time of samples treated with Diaion WA21 resin at 10% (w/v) adsorbent dose using 210 nm detection wavelength. (e) No peak detected at the expected retention time of samples treated with Amberlite IRA-67 resin at 5% (w/v) adsorbent dose using 210 nm detection wavelength. (f) No peak detected at the expected retention time of samples treated with Amberlite IRA-67 resin at 10% (w/v) adsorbent dose using 210 nm detection wavelength. (g) No peak detected at the expected retention time of samples treated with Diaion WA-30 resin at 5% (w/v) adsorbent dose using 210 nm detection wavelength. (h) No peak detected at the expected retention time of samples treated with Diaion WA-30 resin at 10% (w/v) adsorbent dose using 210 nm detection wavelength.

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