Journal of Food Quality / 2018 / Article / Tab 3

Research Article

Saponin Bitterness Reduction of Carica papaya Leaf Extracts through Adsorption of Weakly Basic Ion Exchange Resins

Table 3

Specifications and conditions of saponin detection in C. papaya leaves extract using high-performance liquid chromatography.

SpecificationsConditions

ColumnChromolith performance RP-18e
DetectorUV-VIS
Wavelength210 nm
Temperature23 ± 1°C
Mobile phase (pump A)Deionized water +0.1% (v/v) acetic acid
Mobile phase (pump B)Methanol (HPLC grade)
Flow rate1.0 ml/min
Elution modeBinary gradient
Injection volume20 µl

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