Research Article
Determination of Free Amino Acids in Three Species of Duckweed (Lemnaceae)
Table 3
Calibration curves, LODs, and LOQs for the 24 compounds.
| Number | Compound | Calibration curve | R2 | Linear range (ng/mL) | LOQ (ng/mL) | LOD (ng/mL) |
| 1 | Tyr | y = 6534.1x + 6063.4 | 0.9984 | 1.16–5780 | 0.92 | 0.46 | 2 | Ala | y = 7168.9x + 4932.8 | 0.9969 | 5.30–5300 | 3.71 | 1.24 | 3 | Glu | y = 12796x + 47334 | 0.9960 | 6.41–2564 | 4.49 | 1.50 | 4 | Phe | y = 59157 x + 42917 | 0.9965 | 0.99–992.0 | 0.79 | 0.40 | 5 | His | y = 20263x + 247917 | 0.9962 | 5.57–5570 | 3.90 | 1.30 | 6 | Ile | y = 70857x + 46587 | 0.9957 | 1.07–1074 | 0.86 | 0.43 | 7 | Asp | y = 7084.7x + 86152 | 0.9963 | 5.43–5430 | 3.80 | 1.27 | 8 | Lys | y = 4305.1x + 17648 | 0.9980 | 5.53–5530 | 3.87 | 1.94 | 9 | Gly | y = 1063.0x + 3407.3 | 0.9990 | 0.94–4680 | 0.75 | 0.37 | 10 | Val | y = 31972x + 35728 | 0.9962 | 1.11–1112 | 0.89 | 0.44 | 11 | Cit | y = 8520.8x + 9811.0 | 0.9980 | 1.01–5070 | 0.81 | 0.41 | 12 | GABA | y = 18495x + 14623 | 0.9981 | 1.10–5490 | 0.88 | 0.44 | 13 | Cys2 | y = 2389.7x + 3146.6 | 0.9978 | 1.09–5440 | 0.87 | 0.44 | 14 | Pro | y = 51204x + 58539 | 0.9981 | 1.02–510.0 | 0.82 | 0.41 | 15 | Trp | y = 19030x + 4465.3 | 0.9977 | 1.24–2476 | 0.99 | 0.50 | 16 | Leu | y = 56715x + 40047 | 0.9974 | 1.01–1012 | 0.81 | 0.40 | 17 | Arg | y = 11505x + 84828 | 0.9974 | 5.96–5960 | 4.17 | 1.39 | 18 | Cys | y = 2496.6x + 1017.5 | 0.9988 | 1.20–6012 | 0.96 | 0.48 | 19 | Hpro | y = 15783x + 3968.1 | 0.9977 | 0.95–1892 | 0.76 | 0.38 | 20 | Met | y = 8626.4x + 1545.9 | 0.9984 | 1.05–5250 | 0.84 | 0.42 | 21 | Thr | y = 8807.8x + 19073 | 0.9957 | 4.46–4460 | 3.12 | 1.56 | 22 | Asn | y = 4939.9x + 22367 | 0.9967 | 1.20–5980 | 0.96 | 0.48 | 23 | Gln | y = 7733.9x + 8338.4 | 0.9961 | 0.93–4650 | 0.74 | 0.37 | 24 | Ser | y = 7731.3x + 54859 | 0.9977 | 5.09–5090 | 3.56 | 1.19 |
|
|