Review Article

Three Pillars of Novel Nonthermal Food Technologies: Food Safety, Quality, and Environment

Table 1

Ultrasonic parameters and effects on various bacteria and fungi.

Author(s)Type of foodBacteria and fungiExperimental setup parametersEffects of ultrasound treatment

[73]Skimmed milkEnterobacter aerogenes, Bacillus subtilis, Staphylococcus epidermidis, S. epidermidis, Staphylococcus pseudintermediusν = 20 kHz; P = 13 W; t = 20 min; T = 30°CUp to 4.5 log reduction for E. aerogenes and B. subtilis. Staphylococcus spp. not affected

[74]Skim milk powderGeobacillus stearothermophilusν = 20 kHz, T = 45°C, t = 30 s for cells; ν = 20 kHz, T = 67.5°C, t = 17.5 s for sporesCell reduction (4.8 log) at 19.75% total solids and spore reduction (0.45 log) at 31.5% total solids

[75]Pomegranate juiceEscherichia coli and Saccharomyces cerevisiaeν = 20 kHz; amplitude levels of 50, 75, and 100%; t = 0, 3, 6, 9, 12, 15 min; T = 25 ± 1°C100% amplitude level for 15 min, reduced levels by 3.47 and 1.86 log·cfu/mL, respectively

[76, 77]Strawberry, orange, apple, pineapple, and red fruit juiceSaccharomyces cerevisiae, Pichia membranifaciens, Wickerhamomyces anomalus, Zygosaccharomyces bailii, Zygosaccharomyces rouxii, Candida norvegicaν = 20 kHz; P = 130 W; amplitude levels 20% to 60%; pulse = 2, 6 s; t = 2–6 minReduction of spoilage organisms

[78]Cactus pear juiceEscherichia coliν = 20 kHz; P = 1500 W; amplitude levels of 60%, 70%, 80%, and 90%; t = 1, 3, 5 minTotal inactivation in both fruit juices after 5 min of ultrasound treatment at most amplitude levels

[79]Ayran, an acidic milk drinkStreptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricusν = 35 kHz; T = 60, 70, 80°C; t = 1, 3, 5 minCounts decreased as the temperature and time increased

[80]Orange juiceAlicyclobacillus acidoterrestris sporesν = 24 kHz; AI = 460 W/cm2; P = 33 W; AI = 105 W/cm2; P = 162 WThermosonication required at least 8°C lower temperatures than thermal treatments to achieve the same spore inactivation

[81, 82]Natural squeezed apple juicesAlicyclobacillus acidoterrestris spores and Saccharomyces cerevisiaeν = 20 kHz; P = 600 W and 95.2 μm wave amplitude; t = 10 or 30 min; T = 20, 30, or 44 ± 1°C and pulsed light (PL) (xenon l and 3 pulses/s; 0.1 m distance; 2.4–71.6 Jcm−2; initial T = 20, 30, 44 ± 1°C)Combination of these technologies led up to 3.0 log of spore reduction in commercial apple juice and 2.0 log in natural juice; for S. cerevisiae, 6.4 log reduction and 5.8 log reduction were achieved

[82]Apple juicesEscherichia coli ATCC 35218, Salmonella Enteritidis MA44 and Saccharomyces cerevisiaeν = 20 kHz; P = 600 W and 95.2 μm wave amplitude; pulsed light 0.73 Jcm−2, 155 mL/minCombined ultrasound and pulsed light led up to 3.7–6.3 log reductions of inoculated microorganisms

[83]MilkEscherichia coli, Pseudomonas fluorescens, Staphylococcus aureus, and Debaryomyces hanseniiν = 24 kHz; amplitude levels 70 and 100%; t = 50, 100, 200, 300 sPopulation reduction. Milk deterioration

[75, 84]Pomegranate juiceEscherichia coli and Saccharomyces cerevisiaeν = 20 kHz; amplitudes levels 50, 75, and 100%; t = 0, 6, 12, 18, 24, 30 minMore than a 5 log inactivation of E. coli and a 1.36 log inactivation of S. cerevisiae

[85]Orange juiceSaccharomyces cerevisiaeν = 20 kHz, P = 778.2 W ultrasonic power, t = 11 min; P = 350 W microwave power, T = 35°CComplete inactivation
[86]Fresh produceGram-positive bacterial strain Listeria innocua and Gram-negative bacterial strains Escherichia coli O157:H7 and Pseudomonas fluorescenst < 10 min0.5 log·CFU/cm2 reduction of all strains when washed with ultrasound

[87]ChickenCampylobacter jejuni and spoilage bacteriaT = 4°C, 25°C, 54°C; t = 1, 2, 3 minTreatments significantly reduced total viable counts

[88]SaladsListeria monocytogenest = 5 min in water inoculated with herbs/spicesSamples exhibited up to 4 log reduction 1 day after treatment

ν, frequency (kHz); T, treatment temperature (°C); t, treatment time (min or s); P, treatment power (W); AI, acoustic intensity (W/cm2).