| | Enzyme | Homogenization/other physical treatements | Buffer | Assay | Detection (nm) | Ref. |
| | Glycogen debranching enzyme | 20 min, 18,000 rpm | 137 mM NaCl, 1 mM MgCl2, 1% NP40, 10% glycerol, 2 mM PMSF, 10 mM Na4P2O7, 2.5 mM EDTA, 10 μg/ml Aprotinin, 10 μg g/ml Leupotinin, 100 nM·NaF | Glucose release | PAP kit | [67] | | Ultra tTurrax + centrifugation | 0.05% KHCO3 + 0.004 M EDTA (pH 7.8 at 25 °C) | Glucose release | Iodine reagent, Abs. 525 | [74] |
| | Glycogen phosphorylase | 50 mM Tris–HCl buffer, (pH 7.6). | 50 mM Tris (pH 7.6), 5 mM imidazole, 2 mM EDTA, 10 mM KH2PO4/Na2HPO4 (pH 7.6), 1.4 mM mercaptoethanol, 0.6 mM NADP, 0.05 mM glucose-1,6-diphosphate, 2 mM AMP, 0.65 U phosphoglucomutase, 0.28 U G-6-P dehydrogenase | Abs. increase | Abs. 340 | [67] | | 100 mM K2HPO4 ice-cold, pH 7.4 | 50 mM K2HPO4, 2 mg/mL glycogen, 1.3 mM MgCl2, 0.1 mM EDTA, 0.5 mM NADP, 200 mM MES (pH 6.8), 1 U/mL phosphoglucomutase and 1 U/mL G-6-P dehydrogenase | Abs. increase | Abs. 340 | [75] |
| | AMP- kinase | | | | | |
| | Phosphofructokinase | 100 mM K2HPO4 ice-cold, pH 7.4 | 120 mM MES (pH 6.5), 3.2 mM MgSO4, 2 mM ATP, 0.15 mM AMP, 1 mM NADH, 3 mM fructose 6-phosphate, 2 U/mL triosephosphate isomerase, 1 U/mL glycerol-3-P dehydrogenase and 1 U/mL aldolase | | Abs. 340 | [75] |
| | Pyruvate kinase | 100 mM K2HPO4 ice-cold, pH 7.4 | 120 mM MES (pH 6.5), 100 mM KCl, 10 mM MgCl2, 1.25 mM ADP, 1 mM NADH, 0.5 mM PEP and 2 U/mL lactate dehydrogenase | PEP conc. decrease. | Abs 340 | [75] |
| | AMP deaminase | Ffrozen powdered muscle + 90 mM K2HPO4 pH 6.5, 180 mM KCl, and 0.1 mM dithiothreitol | 200 mM 2-(N-morpholino)ethanesulfonic acid 150 mMKCl, and 10 mMAMP at pH 6.5 | AMP level | HPLC | [75] |
| | Phosphogluco isomerase | 100 mM K2HPO4 pH 7.4 | 200 mM MES, 10 mM Na2HPO4, 5 mM MgCl2, 60 mM KCl, 0.5 mM NAD+, 25 mM glycogen, 25 mM carnosine, 30 mM creatine, 10 mM gG-6-P and 10 mM C2H3NaO2 at pH 5.5–6.0 | F-6-P level | | [75] |
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