Influence of Heat Processing on DNA Degradation and PCR-Based Detection of Wild-Type and Transgenic Maize

Figure 3

PCR analysis of endogenous amplicons with size of (a–d) 226 bp, (e–h) 140 bp, and (i–l) 102 bp from the samples treated at high temperature: 100°C (a, e, i) and 121°C (b–d, f–h, j–l). Exposure duration: lanes 1 and 5 = 0 min; lanes 2 and 6 = 60 min; lanes 3 and 7 = 180 min; lanes 4 and 8 = 300 min. Extraction was performed by the CTAB method (a–b, e–f, i–j) and DNeasy kit (c, d, g, h, k, l). Samples: wild-type maize (lanes 1–4 in (a–l)), event Bt176 (lanes 5–8 in (a–c, e–g, i–k)), event MON810 (lanes 5–8 in (d, h, l)), water (lane 9 in (a–l)), and GelPilot 100 bp ladder (Qiagen) (lane 10 in (a–d)).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)