Influence of Heat Processing on DNA Degradation and PCR-Based Detection of Wild-Type and Transgenic Maize

Table 2

Conditions of PCR.

Primers	Cycles	Initial denaturation		Denaturation		Annealing		Elongation		Final extension
		Temperature (°C)	Time (min)	Temperature (°C)	Time (min)	Temperature (°C)	Time (min)	Temperature (°C)	Time (min)	Temperature (°C)	Time (min)
IVTAS, IVR, ZEIN	40	95	3	95	0.30	63	0.30	72	0.35	72	5
P35S	40	95	3	95	0.30	65	0.30	72	0.35	72	5
Cryl	50	95	3	95	0.25	62	0.30	72	0.45	72	7
Cry124	50	95	3	95	0.25	62	0.30	72	0.35	72	5