Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen
Table 2
Orthogonal experimental results and analysis.
Experiment number
A
B
C
Immobilization ratio of protein (%)
Specific activity (U/μg)
1
1 (3 : 50)
1 (20)
1 (3.5)
87.27 ± 0.42
203.29 ± 14.55
2
1
2 (25)
2 (4.0)
79.33 ± 0.49
226.59 ± 15.14
3
1
3 (30)
3 (4.5)
72.91 ± 0.38
267.33 ± 13.02
4
2 (4 : 50)
1
2
86.08 ± 0.87
169.55 ± 14.83
5
2
2
3
80.51 ± 0.52
180.14 ± 14.25
6
2
3
1
91.39 ± 0.63
158.30 ± 15.54
7
3 (5 : 50)
1
3
82.88 ± 0.54
143.51 ± 19.95
8
3
2
1
94.00 ± 0.78
154.08 ± 15.46
9
3
3
2
89.17 ± 0.44
110.21 ± 19.44
k1-Ir
79.84
85.41
90.89
k2-Ir
85.99
84.61
84.86
k3-Ir
88.68
84.49
78.77
k1-Sa
232.40
172.12
171.89
k2-Sa
169.33
186.94
183.41
k3-Sa
135.93
178.61
196.99
R-Ir
8.84
0.92
12.12
R-Sa
96.47
14.82
25.10
Note: k-Ir means k value of the immobilization ratio of protein; R-Ir means range value of the immobilization ratio of protein; k-Sa means k value of specific activity; R-Sa means range value of specific activity.
