Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen

Table 3

Analysis of variance.

Indicator	Source of variation	SS	df	MS	F
Immobilization ratio of protein	Interblock	129717.013	1	129717.013	121801.63
	A	237.015	2	118.508	111.276
	B	2.688	2	1.344	1.262	F0.05(2,11) = 3.98
	C	440.833	2	220.416	206.966	F0.01(2,11) = 7.21
	Error	11.715	11	1.065
	Total variation	692.251	17
Specific activity	Interblock	578160.136	1	578160.136	812.812
	A	28801.349	2	14400.674	20.245
	B	662.364	2	331.182	0.466	F0.05(2,11) = 3.98
	C	2871.830	2	1435.915	2.019	F0.01(2,11) = 7.21
	Error	7824.394	11	711.309
	Total variation	40159.936	17

Note: significant difference, F ≥ F_0.05(2,11); extremely significant difference, F ≥ F_0.01(2,11).