Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction

Table 3

Detection by digital PCR of mixed samples with known adulteration ratios.

Number	Duck mass (mg)	DNA copy number (copies/μL)			Average value(copies/μL)	Coefficient of variation (%)	Measured cassava mass (mg)	Relative error (%)
1	5.0	481	495	491	489 ± 7.21	1.47	5.35	7
2	10.0	638	632	653	641 ± 10.82	1.69	9.40	−6
3	15.0	849	860	856	855 ± 5.57	0.65	15.16	1.06
4	20.0	1094	1114	1101	1103 ± 10.15	0.92	21.80	9
5	25.0	1290	1276	1280	1282 ± 7.21	0.56	26.60	6.4
6	30.0	1379	1390	1395	1388 ± 8.19	0.59	29.44	−1.86
7	35.0	1601	1623	1609	1611 ± 11.14	0.69	35.42	1.2
8	40.0	1889	1901	1892	1894 ± 6.24	0.33	42.98	7.45
9	45.0	1917	1888	1922	1909 ± 18.36	0.96	43.40	−3.55

Note. Average values are provided as mean ± standard deviation.