Journal of Food Quality

Journal of Food Quality / 2020 / Article
Special Issue

Phenolic Compounds as a Benchmark for Food Quality

View this Special Issue

Research Article | Open Access

Volume 2020 |Article ID 3013649 |

Won-Young Cho, Da-Hee Kim, Ha-Jung Lee, Su-Jung Yeon, Chi-Ho Lee, "Journal of Food Quality Evaluation of Effect of Extraction Solvent on Selected Properties of Olive Leaf Extract", Journal of Food Quality, vol. 2020, Article ID 3013649, 7 pages, 2020.

Journal of Food Quality Evaluation of Effect of Extraction Solvent on Selected Properties of Olive Leaf Extract

Guest Editor: Muhammad K. Khan
Received29 Jul 2019
Revised25 Sep 2019
Accepted25 Oct 2019
Published06 Mar 2020


The quest for natural preservatives and functional foods with health benefits has seen an increasing demand for natural products having therapeutic value. Herein, we investigated the influence of ethanol, methanol, acetone (50%, 70%, and 90% v/v), and distilled water on selected properties of olive leaf extract and determined the yield, total phenolic content (TPC), antioxidant activity, and antimicrobial activity. Extracts were analyzed for their oleuropein, hydroxytyrosol, and tyrosol contents by high-performance liquid chromatography (HPLC). The highest extraction yield of 20.41% was obtained when using 90 vol% methanol, while the highest total polyphenol contents of 232 and 231 mggallic-acid-equivalent/100 g were obtained for 90 vol% methanol and 90 vol% ethanol, respectively. Antioxidant activity was determined using the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging assay, by determining the ferric reducing antioxidant power (FRAP), and using the Fe2+-chelating activity assay, which provided the highest values when 90 vol% methanol was used (33.84%, 0.75, and 12.91%, respectively). HPLC analysis showed that the highest oleuropein contents corresponded to the extracts obtained using 90 and 70 vol% methanol (26.10 ± 0.20 and 24.92 ± 1.22 g/L, respectively), and the highest antimicrobial activity was observed for 90 vol% methanol and distilled water. Olive leaf extracts using 90 vol% methanol had high levels of polyphenols and were highly antioxidant and antimicrobial. The results of this study facilitate the commercial applications of natural extracts with antioxidant and antibacterial activities and are expected to establish a foundation for further optimization studies.

1. Introduction

The increasing demand for natural preservatives and new functional foods with health benefits has inspired numerous studies on biologically active compounds found in plant extracts and the by-products of plant processing [13]. Among these compounds, phenol derivatives (phenolics) exhibit a wide range of physiological effects, including antiallergenic, antiatherogenic, anti-inflammatory, antimicrobial, antioxidant, antithrombotic, anticancer, cardioprotective, and vasodilatory activities [4]. Since phenolics are typically extracted from natural matrices or food industry by-products that are usually discarded or used for animal feed production [5], the influence of solvent on the extraction of phenolics from vegetable substrates has been extensively researched [6]. For example, solvent polarity is known to strongly affect extraction efficiency and other parameters [79]. The differences in the structures of phenolic compounds determine the solubilizing abilities of solvents whose polarities are different. Therefore, the type of extraction solvent and separation procedure can have an important influence on the amounts of polyphenols extracted from plant substances. Although the phenolic contents of food have been widely investigated and extraction conditions optimized for antioxidant activity, some studies have shown that the optimum separation procedure normally depends on the characteristics of the plant [10, 11].

Olive (Olea europaea) fruit, oil, and leaves have a long-standing history of medicinal and nutritional use [12]. Olive leaves are a by-product of olive processing, accounting for up to 10% of the total olive weight, and are considered to be an inexpensive raw material source of antioxidant compounds [13]. Olive leaves have traditionally been used in animal feed, but because they contain high-value compounds with antioxidant and antibacterial properties, they have recently been used as food additives, in functional foods and in pharmaceuticals. [14, 15]. Olive leaves, in particular, exhibit antioxidant, antihypertensive, and anti-inflammatory activities and are effective against hypoglycemia and hypocholesterolemia [16, 17]. The antioxidant activities of olive leaf extracts have been ascribed to the presence of phenolics such as oleuropein, luteolin, and hydroxytyrosol [18]. For example, oleuropein, the main component of olive leaf extract, exhibits antihypotensive, anti-inflammatory, and strong antioxidant activities [1921]. Consequently, there is a growing interest in recovering phenolic compounds from olive leaves [22]. However, the conditions currently used for the extraction of biologically active compounds need to be improved in order to increase extraction efficiency, decrease extraction costs, and preserve functional activity in a better way [23]. In view of the above, in this study, we investigated the effect of the solvent (water, aqueous methanol, aqueous ethanol, and aqueous acetone) used to extract olive leaves on yield, as well as the antioxidant and antimicrobial activities of the extract.

2. Materials and Methods

Olive leaves were imported from Spain (Teetraum, Wollenhaupt Co., Ltd., Germany) and purchased through CJ mall in Korea. Oleuropein, hydroxytyrosol, and tyrosol standards, and α,α-diphenyl-β-picrylhydrazyl (DPPH) were obtained from the Sigma-Aldrich Chemical Co., Korea.

2.1. Preparing the Olive Leaf Extract

Distilled water (DW), aqueous ethanol (50, 70, and 90 vol%), aqueous methanol (50, 70, and 90 vol%), and aqueous acetone (50, 70, and 90 vol%) were used as extraction solvents. Typically, a mixture of dried olive leaf powder (5.0 g) and the solvent of choice (100 mL) were agitated in a shaking incubator at room temperature (25°C) and 250 rpm for 1 h and then centrifuged at 10000 rpm for 10 min. The supernatant was concentrated in vacuo at 50°C using a rotary evaporator, and the residue was freeze-dried.

2.2. Determining Extraction Yield

Extraction yield (%) was calculated as follows: 100% × mExtract/mPowder, where mExtract and mPowder are the masses of the extract and olive leaf powder (g), respectively.

2.3. Determining Total Polyphenol Content (TPC)

TPC was determined using a slight modification of the method reported by Wei et al. [24]. In brief, the test solution (100 μL) was treated with the Folin–Ciocalteu reagent (100 μL, 1 N) and incubated at room temperature for 3 min. The mixture was then treated with aqueous Na2CO3 (300 μL, 1 N), incubated at room temperature for 90 min, and diluted with DW (1 mL). The absorbance of the resulting solution was measured at 725 nm using an OPTIZEN 2120 UV spectrophotometer (Mecasys Co., Ltd., Korea). A standard curve was prepared using 100, 250, 500, and 1000 ppm gallic acid, the results of which were used to calculate the TPC, which is expressed as milligrams of gallic acid equivalents (GAEs) per 100 g (mgGAE/100 g) of the sample. Gallic acid was used as the standard in these experiments. The linear equation for the gallic acid calibration curve can be written as follows: y = 0.0057x - 0.2484 (R2 = 0.991), where y is the TPC and x is the absorbance value.

2.4. α,α-Diphenyl-β-Picrylhydrazyl Radical Scavenging Activity (DPPH), Ferric Reducing Antioxidant Power (FRAP), and Fe2+-Chelating Activity

DPPH radical scavenging activity was determined using the method of Blois [25]. In brief, a solution of DPPH in MeOH (1 mL, 1.5 × 10−4 M) was added to the test solution (4 mL) with stirring, and the resulting mixture was incubated at room temperature for 30 min after which absorbance was measured at 517 nm using the abovementioned spectrophotometer.

FRAP was determined using a slightly modified method reported by Oyaizu [26]. In brief, samples were mixed with sodium phosphate buffer (2.5 mL, 0.2 M, pH 6.6) and K3Fe(CN)6 (2.5 mL, 1% (w/v)). The obtained mixture was incubated at 50°С for 20 min and treated with trichloroacetic acid (2.5 mL, 10% (w/v)). The upper layer of the mixture (2.5 mL) was diluted with DW (2.5 mL) and FeCl3 (0.5 mL, 0.1% (w/v)), after which absorbance was measured at 700 nm using the abovementioned spectrophotometer.

Fe2+-chelating activity was determined using the method of Dinis et al. [27]. Briefly, 0.5 mL of the sample was mixed with a solution of FeCl2 (2 mL, 1 mM) in 95 vol% ethanol. The reaction was initiated by the addition of aqueous ferrozine (2.5 mL, 2 mM), and the mixture was vortexed for 10 min, filtered through a nylon syringe filter (0.45 μm), after which absorbance was measured at 562 nm using the abovementioned spectrophotometer. Fe2+-chelating activity (%) was calculated as follows:

2.5. Quantitating Oleuropein by High-Performance Liquid Chromatography (HPLC)

Oleuropein, hydroxytyrosol, and tyrosol were quantified by HPLC (Agilent 1100 series, USA) after sample filtration through a 0.45 μm PVDF membrane filter (Pall Life Science). The mobile phase contained 5% formic acid (A) and methanol (B), and the following gradient was used: 5% B, then 15% B after 3 min, 25% B after 13 min, 35% B after 25 min, 45% B after 35 min, 50% B after 40 min, 100% B after 45 min, 5% B after 46 min, and re-equilibrate to the initial composition for 4 min. The flow rate was 0.9 mL/min, and elution was performed at room temperature. The injection volume was 10 μL. A Supelcosil LC-ABZ column (250 mm × 4.6 mm, 5 μm) was used, and the absorbance detector was operated at 280 nm.

2.6. Determining Antimicrobial Activity

Total viable counts (TVC) were determined on 3M™ Petrifilm™ aerobic count plates (3M, Seoul, Korea) incubated at 35°C for 24 h. Coliforms and Escherichia coli (E. coli) were determined on 3M™ Petrifilm™ E. coli/coliform count plates (3M, Seoul, Korea) after incubation for 24 h at 35°C. Colonies were identified and counted as per manufacturer’s instructions.

2.7. Statistical Analysis

All experiments were performed in triplicate. Experimental data were analyzed by one-way analysis of variance using SPSS/PC Statistics 23.0 software (SPSS Inc., Chicago, IL, USA). The obtained results are presented as means with corresponding standard deviations. Tukey’s multiple range tests were used to determine significant differences between mean values, and was taken as an indicator of statistical significance.

3. Results and Discussion

3.1. Extraction Yields

Table 1 lists extraction yields and selected properties of the extracts obtained using each solvent. The highest extraction yields of 20.41 and 18.88% were observed for 90 vol% methanol and DW, respectively, while the lowest yield of 10.83% was observed for 90 vol% acetone, which is similar to the trend reported by Butsat and Siriamornpun; when olive leaves were extracted for 6 h using 80% methanol, 80% ethanol, 80% acetone, and DW, the highest extraction yield was observed for 80% methanol and the lowest for 80% acetone [28]. These yields were ascribed to the effect of solvent polarity on the solubilities of the extract components, i.e., proteins and carbohydrates are more soluble in water and methanol than in ethanol or acetone [29].

SolventExtraction yield (%)DPPH radical scavenging activity (%)FRAPFe2+-chelating activity

50%17.55 ± 0.88bc31.17 ± 0.10b0.71 ± 0.01bc9.78 ± 0.12bc
70%17.08 ± 0.77bc31.68 ± 0.40b0.69 ± 0.01c10.93 ± 0.24b
90%17.44 ± 1.13bc31.56 ± 0.19b0.59 ± 0.02d10.06 ± 0.07bc
50%17.17 ± 0.25bc32.97 ± 0.23a0.75 ± 0.01a8.81 ± 0.67cd
70%16.45 ± 0.64bc33.27 ± 0.41a0.76 ± 0.00a9.68 ± 0.81bcd
90%20.41 ± 0.63a33.84 ± 0.47a0.75 ± 0.01a12.91 ± 0.37a
50%16.47 ± 0.84bc31.83 ± 0.13b0.72 ± 0.00b9.12 ± 0.30cd
70%16.03 ± 1.29c31.67 ± 0.35b0.70 ± 0.00bc8.38 ± 0.77de
90%10.83 ± 0.13d31.90 ± 0.22b0.72 ± 0.01b9.19 ± 0.37cd
100% DW18.88 ± 1.83ab26.75 ± 0.46c0.64 ± 0.00e7.22 ± 0.17e

Means with different superscripts (a-ein the same column) differ significantly (). All values are means ± standard deviations from three replicates.
3.2. Total Polyphenol Content (TPC)

Figure 1 shows the effect of solvent on TPC and reveals that the highest values of 231.98 and 230.61 mggae/100 g were obtained for 90 vol% methanol and 90 vol% ethanol, respectively. The lowest TPC of 192.03 mggae/100 g was observed for DW and was significantly different to the values obtained using the other solvents (p < 0.05). TPC was observed to decrease with increasing water content for each solvent, in agreement with previously reported results. Thus, when compared to the other extraction solvents, water results in a higher nonphenolic compound content (e.g., carbohydrates and terpenes) because some phenolic compounds soluble in methanol, ethanol, and acetone can be extracted through complex formation. Hence, compounds that contain more phenol groups or have higher molecular weights than simple phenols are found in the water extract [30]. Moreover, compounds extracted with methanol have been reported to exhibit higher antioxidant activities and phenolic contents than those prepared using other solvents [29, 31], which is consistent with the results of this study. Some researchers have revealed that methanol is typically preferred for the effective extraction of phenolic compounds from plants [32, 33] and that methanol decreases the degeneration of phenols in plant extracts by controlling polyphenol oxidative enzyme activity [34]. Moreover, moudache et al. showed that the TPC content of an olive leaf extract increases with increasing organic content in the extraction solvent [15].

3.3. Antioxidant Activity

The effects of the various solvents on the DPPH radical scavenging activity, FRAP, and Fe2+-chelating activity of the extracts are summarized in Table 1. The first of these parameters is primarily used to quantify the FRAP of natural antioxidants. The original violet color of the DPPH radically changes to yellow when reduced to the corresponding stable diamagnetic molecule. Consequently, determining DPPH radical scavenging activity by observing this color change allows one to characterize numerous samples within a short period, and the method is sensitive enough to detect active ingredients at low concentrations [35]. The highest DPPH radical scavenging activity of 33.84% was observed for 90 vol% methanol; however, the values of 32.97 and 33.27% obtained for 50 and 70 vol% methanol, respectively, were not significantly different (). For aqueous ethanol and aqueous acetone, statistically similar () values of ∼31% were observed, irrespective of water content. The lowest DPPH scavenging activity of 26.75% () was observed for DW.

FRAP is a parameter that quantifies antioxidant activity related to the electron-donating capability of a molecule. The Fe3+ in K3Fe(CN)6 is reduced to Fe2+ in the presence of an antioxidant, which results in the initial yellow test solution turning green or blue [36]. High FRAP values were obtained for all solvents in this study, with the exception of DW, and decreased in the order: aqueous methanol > aqueous acetone > aqueous ethanol >> pure water. The olive leaf methanol extract was determined have strong antioxidant properties; hence, the compounds in this methanol extract are outstanding electron donors capable of terminating oxidation chain reactions by reducing oxidized intermediates to stable forms [37].

Determining Fe2+-chelating activity relies on the ability of the extract to complex Fe2+ ions, thereby inhibiting the formation of the Fe2+-ferrozine complex. The highest Fe2+-chelating activity was obtained for 90 vol% methanol, while the lowest value was obtained for DW (). These findings show that the radical scavenging activity of the olive leaf extract depends on the polarity of the solvent used, in agreement with previous results [38]. Sepúlveda-Jimenez et al. demonstrated that extracts of the same plant origin obtained using methanol exhibited higher antioxidant activities than those extracted with water [39]. Franco et al. showed that the polarity of the extraction solvent strongly influences the extraction efficiency and the antioxidant activities of Rosa rubiginosa and Gevuina avellana extracts [40]. Fractions with different antioxidant activities could be separated on the basis of the polarity of the extracting solvent, with oxygenated compounds selectively extracted in accordance with their chemical structures, polarities, and solubilities [41].

3.4. Analyzing Olive Leaf Extract by HPLC

The oleuropein, hydroxytyrosol, and tyrosol contents of the olive leaf extracts were quantified by HPLC (Table 2, Figure 2), which revealed that 90 vol% methanol was best able to extract these phenolic compounds. Oleuropein has previously been identified as an important component of olive leaf extract [42, 43]. The highest oleuropein content of 26.10 g/L was obtained when extracted with 90 vol% methanol (), while the lowest content of 5.36 g/L was observed for DW, which is the same as the TPC trend. Hydroxytyrosol and tyrosol were detected in considerably smaller amounts, which is in agreement with previous results [44]. Thus, among the tested solvents, methanol was found to be most favorable for extracting oleuropein from olive leaves, which is in agreement with the findings of Bouaziz and Sayadi [18].

SolventCompound (g/L)

50%0.61 ± 0.09abc0.10 ± 0.00ab19.72 ± 1.27bc
70%0.63 ± 0.12abc0.11 ± 0.02a21.96 ± 1.96b
90%0.62 ± 0.09abc0.07 ± 0.01c21.89 ± 1.13b
50%0.72 ± 0.05abc0.07 ± 0.00c19.47 ± 0.85bc
70%0.77 ± 0.03a0.08 ± 0.01bc24.92 ± 1.22a
90%0.74 ± 0.02ab0.07 ± 0.00c26.10 ± 0.20a
50%0.54 ± 0.07bc0.08 ± 0.00c17.70 ± 0.47c
70%0.52 ± 0.06c0.03 ± 0.01d17.66 ± 0.64c
90%0.61 ± 0.10abc0.02 ± 0.00d17.79 ± 0.48c
100% DW0.25 ± 0.02d0.03 ± 0.00d5.36 ± 0.78d

Means with different superscripts (a-din the same column) differ significantly (). All values are means ± standard deviations for three replicates.
3.5. Antimicrobial Activity

Table 3 shows the antimicrobial activities of fractions extracted with various solvents, which reveals that antimicrobial activity decreases in the order: DW > 90 vol% methanol >70 vol% methanol >90 vol% ethanol >90 vol% acetone. The above extracts were examined for their effectiveness against experimental microorganisms. All extract did not detect in coliform count plate and E. coli count plate (data not shown). These findings are in agreement with the previously reported abilities of olive leaf extract to inhibit the growth of certain pathogenic bacteria [45, 46]. The observed antimicrobial activities are attributable to the phenolic contents of the extracts [47, 48]; the high contents of oleuropein and other phenolic compounds identified in the extracts contribute to the observed antibacterial properties. In this study, the total viable counts of the 90 vol% methanol, ethanol, and acetone extracts are low because of the high phenol contents of these extracts. Oleuropein has been reported to improve the production of nitric oxide in a dose-dependent manner (it is known to be cytotoxic to various pathogenic bacteria) in endotoxin-challenged mouse macrophages [49]. The effects of oleuropein and its derivatives contribute to the in vivo defense system against bacterial infection. The total phenolic content and the amount of oleuropein determined by HPLC in the DW extract were the lowest; however, this extract exhibited the highest antimicrobial activity of 33.33 CFU/mL. According to previous reports, antimicrobial activity is not only related to the total phenol content, but also to the types and relative distributions of the phenolic components, which are important for biological activity. Bacterial resistance is also related to the structure of the polyphenol. Therefore, the compounds in the DW extract need to be identified through further studies.

SolventAntimicrobial (CFU/mL)

50%771.67 ± 34.03b
70%756.67 ± 91.70b
90%145.00 ± 56.35c
50%598.33 ± 117.30b
70%116.67 ± 2.89c
90%36.67 ± 18.93c
50%1070.00 ± 105.00a
70%780.00 ± 173.86b
90%165.00 ± 17.32c
100% DW33.33 ± 16.07c

Means with different superscripts (a-cin the same column) differ significantly (). All values are means ± standard deviations for three replicates.

4. Conclusions

We determined optimal conditions for olive leaf extraction by examining the effect of extraction solvent on selected extract properties. The highest extraction efficiency of 20.41% was obtained using 90 vol% methanol, while the lowest value of 18.88% was obtained using DW, and the highest total polyphenol contents were obtained with 90 vol% methanol and 90 vol% ethanol, while the lowest was obtained using DW (). The highest antioxidant activity was observed for the extract obtained using 90 vol% methanol, and the oleuropein content was highest when 90 and 70 vol% methanol were used as the extraction solvents. Finally, extracts with the highest antimicrobial activities were obtained using 90 vol% methanol and DW. Thus, we conclude that 90 vol% methanol is the optimal extraction solvent, affording extracts with high antioxidant and antibacterial activities in high yields. The results of this study are expected to be of importance for the development of a wide range of products based on olive leaf extract.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare no conflicts of interest.


  1. G. Joana Gil-Chávez, J. A. Villa, J. Fernando Ayala-Zavala et al., “Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: an overview,” Comprehensive Reviews in Food Science and Food Safety, vol. 12, no. 1, pp. 5–23, 2013. View at: Publisher Site | Google Scholar
  2. A. Ribeiro, M. Estanqueiro, M. Oliveira, and J. Sousa Lobo, “Main benefits and applicability of plant extracts in skin care products,” Cosmetics, vol. 2, no. 2, pp. 48–65, 2015. View at: Publisher Site | Google Scholar
  3. C. Soler-Rivas, J. C. Espín, and H. J. Wichers, “Oleuropein and related compounds,” Journal of the Science of Food and Agriculture, vol. 80, no. 7, pp. 1013–1023, 2000. View at: Publisher Site | Google Scholar
  4. N. Balasundram, K. Sundram, and S. Samman, “Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses,” Food Chemistry, vol. 99, no. 1, pp. 191–203, 2006. View at: Publisher Site | Google Scholar
  5. M. Herrero, T. N. Temirzoda, A. Segura-Carretero, R. Quirantes, M. Plaza, and E. Ibañez, “New possibilities for the valorization of olive oil by-products,” Journal of Chromatography A, vol. 1218, no. 42, pp. 7511–7520, 2011. View at: Publisher Site | Google Scholar
  6. M. Pinelo, M. Rubilar, J. Sineiro, and M. J. Núñez, “Extraction of antioxidant phenolics from almond hulls (Prunus amygdalus) and pine sawdust (Pinus pinaster),” Food Chemistry, vol. 85, no. 2, pp. 267–273, 2004. View at: Publisher Site | Google Scholar
  7. L. M. Cheung, P. C. K. Cheung, and V. E. C. Ooi, “Antioxidant activity and total phenolics of edible mushroom extracts,” Food Chemistry, vol. 81, no. 2, pp. 249–255, 2003. View at: Publisher Site | Google Scholar
  8. R. P. Singh, K. N. Chidambara Murthy, and G. K. Jayaprakasha, “Studies on the antioxidant activity of pomegranate (punicagranatum) peel and seed extracts using in vitro models,” Journal of Agricultural and Food Chemistry, vol. 50, no. 1, pp. 81–86, 2002. View at: Publisher Site | Google Scholar
  9. B. Sultana, F. Anwar, and M. Ashraf, “Effect of extraction Solvent/Technique on the antioxidant activity of selected medicinal plant extracts,” Molecules, vol. 14, no. 6, pp. 2167–2180, 2009. View at: Publisher Site | Google Scholar
  10. T. M. Rababah, F. Banat, A. Rababah, K. Ereifej, and W. Yang, “Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate,” Journal of Food Science, vol. 75, no. 7, pp. C626–C632, 2010. View at: Publisher Site | Google Scholar
  11. N. Pellegrini, B. Colombi, S. Salvatore et al., “Evaluation of antioxidant capacity of some fruit and vegetable foods: efficiency of extraction of a sequence of solvents,” Journal of the Science of Food and Agriculture, vol. 87, no. 1, pp. 103–111, 2007. View at: Publisher Site | Google Scholar
  12. M. G. Soni, G. A. Burdock, M. S. Christian, C. M. Bitler, and R. Crea, “Safety assessment of aqueous olive pulp extract as an antioxidant or antimicrobial agent in foods,” Food and Chemical Toxicology, vol. 44, no. 7, pp. 903–915, 2006. View at: Publisher Site | Google Scholar
  13. J. Tabera, Á. Guinda, A. Ruiz-Rodríguez et al., “Countercurrent supercritical fluid extraction and fractionation of high-added-value compounds from a hexane extract of olive leaves,” Journal of Agricultural and Food Chemistry, vol. 52, no. 15, pp. 4774–4779, 2004. View at: Publisher Site | Google Scholar
  14. E. Roselló-Soto, M. Koubaa, A. Moubarik et al., “Emerging opportunities for the effective valorization of wastes and by-products generated during olive oil production process: non-conventional methods for the recovery of high-added value compounds,” Trends in Food Science & Technology, vol. 45, no. 2, pp. 296–310, 2015. View at: Publisher Site | Google Scholar
  15. M. Moudache, M. Colon, C. Nerín, and F. Zaidi, “Phenolic content and antioxidant activity of olive by-products and antioxidant film containing olive leaf extract,” Food Chemistry, vol. 212, pp. 521–527, 2016. View at: Publisher Site | Google Scholar
  16. F. Brahmi, B. Mechri, S. Dabbou, M. Dhibi, and M. Hammami, “The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations,” Industrial Crops and Products, vol. 38, pp. 146–152, 2012. View at: Publisher Site | Google Scholar
  17. S. N. El and S. Karakaya, “Olive tree (Olea europaea) leaves: potential beneficial effects on human health,” Nutrition Reviews, vol. 67, no. 11, pp. 632–638, 2009. View at: Publisher Site | Google Scholar
  18. M. Bouaziz, I. Fki, H. Jemai, M. Ayadi, and S. Sayadi, “Effect of storage on refined and husk olive oils composition: stabilization by addition of natural antioxidants from Chemlali olive leaves,” Food Chemistry, vol. 108, no. 1, pp. 253–262, 2008. View at: Publisher Site | Google Scholar
  19. M. T. Khayyal, M. el Ghazaly, D. Abdallah, N. Nassar, S. Okpanyi, and M. H. Kreuter, “Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats,” Arzneimittelforschung, vol. 52, no. 11, pp. 797–802, 2002. View at: Google Scholar
  20. J. M. Martínez-Martos, M. D. Mayas, P. Carrera et al., “Phenolic compounds oleuropein and hydroxytyrosol exert differential effects on glioma development via antioxidant defense systems,” Journal of Functional Foods, vol. 11, pp. 221–234, 2014. View at: Publisher Site | Google Scholar
  21. C. Puel, J. Mathey, A. Agalias et al., “Dose-response study of effect of oleuropein, an olive oil polyphenol, in an ovariectomy/inflammation experimental model of bone loss in the rat,” Clinical Nutrition, vol. 25, no. 5, pp. 859–868, 2006. View at: Publisher Site | Google Scholar
  22. M. H. Ahmad-Qasem, J. Cánovas, E. Barrajón-Catalán, V. Micol, J. A. Cárcel, and J. V. García-Pérez, “Kinetic and compositional study of phenolic extraction from olive leaves (var. Serrana) by using power ultrasound,” Innovative Food Science & Emerging Technologies, vol. 17, pp. 120–129, 2013. View at: Publisher Site | Google Scholar
  23. D. Cifá, M. Skrt, P. Pittia, C. Di Mattia, and N. Poklar Ulrih, “Enhanced yield of oleuropein from olive leaves using ultrasound-assisted extraction,” Food Science & Nutrition, vol. 6, no. 4, pp. 1128–1137, 2018. View at: Publisher Site | Google Scholar
  24. X. Wei, M. Luo, L. Xu et al., “Production of fibrinolytic enzyme from Bacillus amyloliquefaciens by fermentation of chickpeas, with the evaluation of the anticoagulant and antioxidant properties of chickpeas,” Journal of Agricultural and Food Chemistry, vol. 59, no. 8, pp. 3957–3963, 2011. View at: Publisher Site | Google Scholar
  25. M. S. Blois, “Antioxidant determinations by the use of a stable free radical,” Nature, vol. 181, no. 4617, pp. 1199-1200, 1958. View at: Publisher Site | Google Scholar
  26. M. Oyaizu, “Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine,” The Japanese Journal of Nutrition and Dietetics, vol. 44, no. 6, pp. 307–315, 1986. View at: Publisher Site | Google Scholar
  27. T. C. P. Dinis, V. M. C. Madeira, and L. M. Almeida, “Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers,” Archives of Biochemistry and Biophysics, vol. 315, no. 1, pp. 161–169, 1994. View at: Publisher Site | Google Scholar
  28. S. Butsat and S. Siriamornpun, “Effect of solvent types and extraction times on phenolic and flavonoid contents and antioxidant activity in leaf extracts of Amomum chinense C,” International Food Research Journal, vol. 23, no. 1, pp. 180–187, 2016. View at: Google Scholar
  29. H. Zielinski and H. Kozlowska, “Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions,” Journal of Agriculture and Food Chemistry, vol. 48, no. 6, pp. 2008–2016, 2000. View at: Publisher Site | Google Scholar
  30. Q. D. Do, A. E. Angkawijaya, P. L. Tran-Nguyen et al., “Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica,” Journal of Food and Drug Analysis, vol. 22, no. 3, pp. 296–302, 2014. View at: Publisher Site | Google Scholar
  31. F. Sosulski, K. Krygier, and L. Hogge, “Free, esterified, and insoluble-bound phenolic acids. 3. Composition of phenolic acids in cereal and potato flours,” Journal of Agricultural and Food Chemistry, vol. 30, no. 2, pp. 337–340, 1982. View at: Publisher Site | Google Scholar
  32. F. Ye, Q. Liang, H. Li, and G. Zhao, “Solvent effects on phenolic content, composition, and antioxidant activity of extracts from florets of sunflower (Helianthus annuus L.),” Industrial Crops and Products, vol. 76, no. 15, pp. 574–581, 2015. View at: Publisher Site | Google Scholar
  33. U.-J. Vajic, G.-M. Jelica, J. Zivkovic et al., “Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology,” Industrial Crops and Products, vol. 74, no. 15, pp. 912–917, 2015. View at: Google Scholar
  34. D. Tura and K. Robards, “Sample handling strategies for the determination of biophenols in food and plants,” Journal of Chromatography A, vol. 975, no. 1, pp. 71–93, 2002. View at: Publisher Site | Google Scholar
  35. Y.-C. Hseu, W.-H. Chang, C.-S. Chen et al., “Antioxidant activities of toona sinensis leaves extracts using different antioxidant models,” Food and Chemical Toxicology, vol. 46, no. 1, pp. 105–114, 2008. View at: Publisher Site | Google Scholar
  36. J. Liu, C. Wang, Z. Wang, C. Zhang, S. Lu, and J. Liu, “The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides,” Food Chemistry, vol. 126, no. 1, pp. 261–269, 2011. View at: Publisher Site | Google Scholar
  37. S. Tachakittirungrod, S. Okonogi, and S. Chowwanapoonpohn, “Study on antioxidant activity of certain plants in Thailand: mechanism of antioxidant action of guava leaf extract,” Food Chemistry, vol. 103, no. 2, pp. 381–388, 2007. View at: Publisher Site | Google Scholar
  38. L. Abaza, N. Ben Youssef, H. Manai, F. Mahjoub Haddada, K. Methenni, and M. Zarrouk, “Chétoui olive leaf extracts: influence of the solvent type on phenolics and antioxidant activities,” Grasas Y Aceites, vol. 62, no. 1, pp. 96–104, 2011. View at: Publisher Site | Google Scholar
  39. G. Sepulveda-, C. Reyna-Aqui, L. Chaires-Ma, K. Bermudez-T, and M. Rodriguez-, “Antioxidant activity and content of phenolic compounds and flavonoids from Justicia spicigera,” Journal of Biological Sciences, vol. 9, no. 6, pp. 629–632, 2009. View at: Publisher Site | Google Scholar
  40. D. Franco, J. Sineiro, M. Rubilar et al., “Polyphenols from plant materials: extraction and antioxidant power,” Electronical Journal of Environmental, Agricultural and Food Chemistry, vol. 7, no. 8, pp. 3210–3216, 2008. View at: Google Scholar
  41. N. G. T. Meneses, S. Martins, J. A. Teixeira, and S. I. Mussatto, “Influence of extraction solvents on the recovery of antioxidant phenolic compounds from brewer’s spent grains,” Separation and Purification Technology, vol. 108, no. 19, pp. 152–158, 2013. View at: Publisher Site | Google Scholar
  42. E. Altıok, D. Baycın, O. Bayraktar, and S. Ulku, “Isolation of polyphenols from the extracts of olive leaves (Oleaeuropaea L.) by adsorption on silk fibroin,” Separation and Purification Technology, vol. 62, no. 2, pp. 342–348, 2008. View at: Publisher Site | Google Scholar
  43. O. Benavente-Garcia, J. Castillo, J. Lorente, O. Ortuno, and J. A. Del Rio, “Antioxidant activity of phenolics extracted from Oleaeuropaea L. leaves,” Food Chemistry, vol. 68, no. 4, pp. 457–462, 2000. View at: Google Scholar
  44. M. A. Temiz and A. Temur, “Effect of solvent variation on polyphenolic profile and total phenolic content of olive leaf extract,” Yuzuncu Yil University Journal of Agricultural Sciences, vol. 27, no. 1, pp. 43–50, 2017. View at: Google Scholar
  45. M. A. Aliabadi, R. K. Darsanaki, M. L. Rokhi, M. Nourbakhsh, and G. Raeisi, “Antimicrobial activity of olive leaf aqueous extract,” Annals of Biological Research, vol. 3, no. 8, pp. 4189–4191, 2012. View at: Google Scholar
  46. V. Marsilio and B. Lanza, “Characterisation of an oleuropein degrading strain ofLactobacillus plantarum. Combined effects of compounds present in olive fermenting brines (phenols, glucose and NaCl) on bacterial activity,” Journal of the Science of Food and Agriculture, vol. 76, no. 4, pp. 520–524, 1998. View at: Publisher Site | Google Scholar
  47. J. A. Pereira, A. P. G. Pereira, I. C. F. R. Ferreira et al., “Table olives from Portugal: phenolic compounds, antioxidant potential, and antimicrobial activity,” Journal of Agricultural and Food Chemistry, vol. 54, no. 22, pp. 8425–8431, 2006. View at: Publisher Site | Google Scholar
  48. C. Proestos, N. Chorianopoulos, G.-J. E. Nychas, and M. Komaitis, “RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity,” Journal of Agricultural and Food Chemistry, vol. 53, no. 4, pp. 1190–1195, 2005. View at: Publisher Site | Google Scholar
  49. F. Visioli, S. Bellosta, and C. Galli, “Oleuropein, the bitter principle of olives, enhances nitric oxide production by mouse macrophages,” Life Sciences, vol. 62, no. 2, pp. 541–546, 1998. View at: Publisher Site | Google Scholar

Copyright © 2020 Won-Young Cho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Related articles

No related content is available yet for this article.
 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

No related content is available yet for this article.

Article of the Year Award: Outstanding research contributions of 2021, as selected by our Chief Editors. Read the winning articles.