Preservative Effect of Novel Combined Treatment with Electrolyzed Active Water and Lysozyme Enzyme to Increase the Storage Life of Vacuum-Packaged Carp

Table 2

Effects of washing, acidic electrolyzed oxidizing water, and lysozyme treatments on the mesophilic anaerobic plate count of carp samples.

Treatment	Mesophilic anaerobic plate count of carp samples (log10 CFU/g ± SD)
	Day 0	Day 1	Day 2	Day 4	Day 7
Control	4.39 ± 0.20^a	5.75 ± 0.17^a	6.51 ± 0.27^a	6.55 ± 0.26^a	7.29 ± 0.15^a
Washed	3.85 ± 0.22^b	5.41 ± 0.12^a	6.03 ± 0.17^b	6.75 ± 0.28^a	7.31 ± 0.50^a
AEO	2.76 ± 0.58^cd	3.30 ± 0.43^c	4.30 ± 0.33^d	5.14 ± 0.40^c	5.89 ± 0.05^b
AEO washed	3.16 ± 0.57^c	4.11 ± 0.34^b	4.63 ± 0.18^c	5.86 ± 0.08^b	6.92 ± 0.11^a
AEO + L	2.29 ± 0.26^d	2.95 ± 0.26^c	3.61 ± 0.26^e	4.57 ± 0.21^d	5.28 ± 0.16^c
AEO washed + L	2.84 ± 0.08^c	4.07 ± 0.55^b	4.23 ± 0.15^d	5.21 ± 0.09^c	5.86 ± 0.12^b

^aMean ± SD, n = 5; values in the same column for each attribute followed by a different letter (a–d) are significantly different (). AEO: acidic electrolyzed oxidizing (AEO) water (100 mg/kg) treatment for 5 min. AEO washed: acidic electrolyzed oxidizing water treatment for 5 min followed by washing. AEO + L: electrolyzed oxidizing water treatment for 5 min followed by spraying of 0.5% lysozyme enzyme (L) solution. AEO washed + L: electrolyzed oxidizing water treatment for 5 min followed by washing and spraying of 0.5% lysozyme enzyme solution on the total viable count of carp samples.