Preservative Effect of Novel Combined Treatment with Electrolyzed Active Water and Lysozyme Enzyme to Increase the Storage Life of Vacuum-Packaged Carp

Table 3

Effects of washing, acidic electrolyzed oxidizing water, and lysozyme treatments on the Enterobacteriaceae count of carp samples.

Treatment	Enterobacteriaceae count of carp samples (log10 CFU/g ± SD)
	Day 0	Day 1	Day 2	Day 4	Day 7
Control	3.49 ± 0.30^a	4.58 ± 0.28^a	6.16 ± 0.53^a	7.41 ± 0.26^a	8.04 ± 0.12^a
Washed	2.75 ± 0.39^b	4.36 ± 0.18^a	5.99 ± 0.20^a	6.98 ± 0.05^a	7.93 ± 0.12^a
AEO	1.72 ± 0.03^d	2.79 ± 0.20^bc	4.20 ± 0.41^b	4.53 ± 0.48^c	4.66 ± 0.28^d
AEO washed	2.33 ± 0.03^c	3.05 ± 0.40^b	4.26 ± 0.16^b	5.49 ± 0.35^b	6.98 ± 0.32^b
AEO + L	1.30 ± 0.32^e	2.16 ± 0.28^d	3.03 ± 0.13^c	3.73 ± 0.10^d	4.58 ± 0.27^d
AEO washed + L	1.89 ± 0.49^d	2.61 ± 0.30^c	4.01 ± 0.29^b	4.76 ± 0.56^d	5.06 ± 0.43^d

^aMean ± SD, n = 5; values in the same column for each attributes followed by a different letter (a–d) are significantly different (). AEO: acidic electrolyzed oxidizing (AEO) water (100 mg/kg) treatment for 5 min. AEO washed: acidic electrolyzed oxidizing water treatment for 5 min followed by washing. AEO + L: electrolyzed oxidizing water treatment for 5 min followed by spraying of 0.5% lysozyme enzyme (L) solution. AEO washed + L: electrolyzed oxidizing water treatment for 5 min followed by washing and spraying of 0.5% lysozyme enzyme solution on the total viable count of carp samples.