Journal of Food Quality

Research Article

Effects of 1-Methylcyclopropene and Controlled Atmosphere on Ethylene Synthesis and Quality Attributes of Avocado cvs. Edranol and Fuerte

Table 4

Gene expression analysis of PamACO, PamACS1, and PamACS2 transcript in “Fuerte” avocado under different storage conditions for 30 and 50 days at 5°C. Fruit was harvested at two maturity stages.


Harvest	Storage time (days)	Treatment	ACO			ACS1			ACS2
Harvest	Storage time (days)	Treatment	Mean		s.d	Mean		s.d	Mean		s.d

I	0	Control	0.02	±	0.09	0.020	±	0.01	1.63	±	2.05
	30 d	RA	9.07	±	4.31^b	9.85	±	7.42^b	1.50	±	0.48^a
		CA	0.79	±	0.41^a	0.83	±	0.40^a	0.81	±	0.41^a
		RA + 1-MCP	0.41	±	0.30^a	0.57	±	0.63^a	0.68	±	0.48^a
	50 d	RA	9.36	±	3.05^b	12.70	±	5.37^b	1.20	±	0.56^a
		CA	2.03	±	0.70^a	1.60	±	0.38^a	4.77	±	6.54^a
		RA + 1-MCP	2.51	±	1.94^a	3.44	±	3.56^a	0.65	±	0.19^a

II	0	Control	0.01	±	0.001	0.01	±	0.00	0.00	±	0.00
	30 d	RA	9.10	±	0.80^b	8.10	±	4.10^b	0.00	±	0.00^a
		CA	0.50	±	0.18^a	0.73	±	0.06^a	0.00	±	0.00^a
		RA + 1-MCP	2.18	±	0.86^a	1.89	±	0.73^a	0.00	±	0.00^a
	50 d	RA	2.40	±	1.93^a	3.62	±	3.12^a	0.00	±	0.00^a
		CA	2.00	±	1.51^a	1.60	±	0.66^a	0.00	±	0.00^a
		RA + 1-MCP	8.40	±	2.50^b	14.17	±	3.92^b	0.00	±	0.00^a

Interaction
Treatment (A)				<0.0001			<0.0001			0.4386
Storage time (B)				0.0751			0.0083			0.2649
Harvest (C)				0.6316			0.8529			0.0023
A × B				0.001			0.0012			0.2004
A × C				<0.0001			0.0001			0.4386
B × C				0.9149			0.6898			0.2649
A × B × C				0.0228			0.0023			0.2004

RA: regular air (85% relative humidity); CA: controlled atmosphere (4% O₂ + 6% CO₂); 1-MCP: 1-methylcyclopropene (300 ppb); s.d: standard deviation. I: early maturity stage; II: advanced maturity stage. d: days at 5°C. The accumulation of PamACO, PamACS1, and PamACS2 was measured by qPCR in fruit at harvest and after cold storage in different treatments. Expression was normalized with the house-keeping gene PamTCPB. Each value corresponds to mean of three fruits ± sd. Different letters within each storage time and harvest represents significant differences at as determined by the LSD test. Factor: treatment: RA, CA, and RA + 1-MCP. Storage time: fruits storage for 30 and 50 days at 5°C. Harvest: two harvest, early and advanced maturity stages. Factor significant, .