Journal of Food Quality

Research Article

Effects of 1-Methylcyclopropene and Controlled Atmosphere on Ethylene Synthesis and Quality Attributes of Avocado cvs. Edranol and Fuerte

Table 5

Gene expression analysis of PamACO, PamACS1, and PamACS2 transcript in avocado “Edranol” under different storage conditions for 30 and 50 days at 5°C. Fruit was harvested at two maturity stages.


Harvest	Storage time (days)	Treatment	ACO			ACS1			ACS2
Harvest	Storage time (days)	Treatment	Mean		s.d	Mean		s.d	Mean		s.d

I	0	Control	0.03	±	0.01	0.01	±	0.01	2.99	±	1.24
	30 d	RA	13.59	±	6.60^b	6.63	±	2.63^b	1.10	±	0.24^a
		CA	0.27	±	0.04^a	0.26	±	0.02^a	0.53	±	0.25^a
		RA + 1-MCP	0.63	±	0.13^a	1.05	±	0.19^a	0.60	±	0.30^a
	50 d	RA	17.45	±	2.82^b	27.6	±	0.93^b	1.04	±	0.20^a
		CA	1.18	±	0.18^a	0.90	±	0.20^a	0.88	±	0.44^a
		RA + 1-MCP	1.35	±	0.17^a	3.02	±	0.34^a	0.73	±	0.25^a

II	0	Control	0.01	±	0.01	0.11	±	0.04	0.87	±	0.84
	30 d	RA	15.97	±	6.60^b	14.28	±	1.83^b	0.77	±	0.47^a
		CA	0.24	±	0.07^a	0.77	±	0.27^a	0.74	±	0.55^a
		RA + 1-MCP	0.77	±	0.14^a	0.88	±	0.10^a	0.90	±	0.62^a
	50 d	RA	9.04	±	1.62^b	4.67	±	0.98^b	2.59	±	1.57^a
		CA	0.88	±	0.41^a	0.03	±	0.01^a	1.12	±	0.73^a
		RA + 1-MCP	3.55	±	1.76^a	7.37	±	2.56^c	2.45	±	1.88^a

Interaction
Treatment (A)				<0.0001			<0.0001			0.6029
Storage time (B)				0.3587			0.0386			0.0137
Harvest (C)				0.9717			0.7575			0.0385
A × B				0.7862			0.266			0.7672
A × C				0.6695			0.2665			0.1003
B × C				0.0305			0.0022			0.0609
A × B × C				0.0009			<0.0001			0.3244

RA: regular air (85% relative humidity); CA: controlled atmosphere (4% O₂ + 6% CO₂); 1-MCP: 1-methylcyclopropene (300 ppb); s.d: standard deviation. I: early maturity stage; II: advanced maturity stage; d: days at 5°C. The accumulation of PamACO, PamACS1, and PamACS2 was measured by qPCR in fruit at harvest and after cold storage in different treatments. Expression was normalized with the house-keeping gene PamTCPB. Each value corresponds to mean of three fruits ± sd. Different letters within each storage time and harvest represent significant differences at as determined by the LSD test. Factor: treatment: RA, CA, and RA + 1-MCP. Storage time: fruits storage for 30 and 50 days at 5°C. Harvest: two harvest, early and advanced maturity stages. Factor significant, .