Quantitation of Phenolic Compounds Related to Antioxidant and Antiosteoporosis Activities in Ripe and Unripe Maesil (Prunus mume)

Table 3

Method validation results of the developed method for analysis of compounds 1–9 in samples.

Compounds	Range; (μg/mL)	Linearity; (R^2)	Calibration; curves	LODs; (μg/mL)	LOQs; (μg/mL)	Concentration; (μg/mL)	Intraday (n = 3)		Interday (n = 5)		Original concentration; (μg/mL)	Spiked concentration; (μg/mL)	Recovery; (%)	RSD; (%)
							Precision (%RSD)	Accuracy (%)	Precision (%RSD)	Accuracy (%)
1	16–500	0.9994	y = 1.78x + 3.82	2.12	7.06	16	2.1	98.8 ± 2.1	1.6	102.9 ± 1.6	214	171	98.1	2.9
						63	0.9	100.0 ± 0.9	1.6	102.6 ± 1.6		214	103.1	2.9
						250	0.5	100.3 ± 0.5	0.1	100.1 ± 0.1		257	100.1	0.3
2	16–250	0.9996	y = 1.58x + 0.91	0.72	2.41	16	0.0	100.0 ± 0.0	2.0	101.5 ± 2.0	34	27	100.0	1.9
						63	1.1	100.6 ± 1.1	1.8	98.6 ± 1.8		34	101.0	1.9
						250	0.2	99.8 ± 0.2	0.4	99.7 ± 0.4		41	101.3	2.1
3	16–250	0.9997	y = 0.47x + 0.09	3.80	11.53	16	2.9	101.9 ± 3.0	2.4	101.4 ± 2.5	120	96	102.1	3.7
						63	0.0	100.0 ± 0.0	2.7	102.7 ± 2.8		120	99.8	3.3
						250	0.8	101.7 ± 0.8	2.3	101.4 ± 2.3		144	101.8	1.0
4	16–250	0.9992	y = 0.77x + 0.07	0.37	1.24	16	0.0	100.0 ± 0.0	3.2	109.2 ± 3.4	45	36	100.0	0.9
						63	1.1	100.7 ± 1.1	2.9	99.6 ± 2.9		45	100.0	1.7
						250	0.3	99.8 ± 0.3	1.2	100.3 ± 1.2		54	99.5	1.2
5	8–250	0.9995	y = 4.69x + 8.50	0.64	1.95	16	1.4	99.2 ± 1.3	1.9	100.5 ± 2.0	11	9	101.0	3.9
						63	1.2	100.5 ± 1.2	1.5	98.9 ± 1.5		11	103.3	0.9
						250	0.2	99.8 ± 0.2	1.3	99.5 ± 1.3		13	102.7	0.7
6	16–250	0.9996	y = 1.36x + 1.08	1.96	6.55	16	0.0	100.0 ± 0.0	1.9	103.5 ± 1.9	49	39	102.6	3.6
						63	0.6	100.4 ± 0.6	1.7	99.1 ± 1.7		49	103.0	0.9
						250	0.3	99.7 ± 0.3	0.3	99.8 ± 0.3		59	102.6	1.7
7	16–250	0.9996	y = 1.19x + 0.94	0.61	2.04	16	0.0	100.0 ± 0.0	2.7	103.0 ± 2.7	21	17	101.6	3.2
						63	0.7	100.4 ± 0.7	2.8	99.8 ± 2.7		21	101.1	1.6
						250	0.2	99.9 ± 1.2	1.7	100.6 ± 1.8		25	100.4	1.3
8	16–250	0.9995	y = 1.04x + 0.75	1.60	5.33	16	0.0	100.0 ± 0.0	3.0	102.2 ± 3.0	106	85	104.3	5.2
						63	0.8	100.5 ± 0.8	2.2	99.4 ± 2.2		106	100.2	2.9
						250	0.2	99.9 ± 0.2	1.4	99.6 ± 1.4		127	102.3	1.3
9	16–250	0.9997	y = 9.13x + 13.67	3.85	12.82	16	3.9	96.7 ± 3.8	0.3	99.6 ± 0.3	40	32	101.6	2.6
						63	1.1	100.2 ± 1.1	0.3	99.7 ± 0.3		40	100.6	2.9
						250	0.1	99.9 ± 0.1	0.2	99.8 ± 0.2		48	103.2	1.7