Research Article
Quantitation of Phenolic Compounds Related to Antioxidant and Antiosteoporosis Activities in Ripe and Unripe Maesil (Prunus mume)
Table 3
Method validation results of the developed method for analysis of compounds 1–9 in samples.
| Compounds | Range (μg/mL) | Linearity (R2) | Calibration curves | LODs (μg/mL) | LOQs (μg/mL) | Concentration (μg/mL) | Intraday (n = 3) | Interday (n = 5) | Original concentration (μg/mL) | Spiked concentration (μg/mL) | Recovery (%) | RSD (%) | Precision (%RSD) | Accuracy (%) | Precision (%RSD) | Accuracy (%) |
| 1 | 16–500 | 0.9994 | y = 1.78x + 3.82 | 2.12 | 7.06 | 16 | 2.1 | 98.8 ± 2.1 | 1.6 | 102.9 ± 1.6 | 214 | 171 | 98.1 | 2.9 | 63 | 0.9 | 100.0 ± 0.9 | 1.6 | 102.6 ± 1.6 | 214 | 103.1 | 2.9 | 250 | 0.5 | 100.3 ± 0.5 | 0.1 | 100.1 ± 0.1 | 257 | 100.1 | 0.3 | 2 | 16–250 | 0.9996 | y = 1.58x + 0.91 | 0.72 | 2.41 | 16 | 0.0 | 100.0 ± 0.0 | 2.0 | 101.5 ± 2.0 | 34 | 27 | 100.0 | 1.9 | 63 | 1.1 | 100.6 ± 1.1 | 1.8 | 98.6 ± 1.8 | 34 | 101.0 | 1.9 | 250 | 0.2 | 99.8 ± 0.2 | 0.4 | 99.7 ± 0.4 | 41 | 101.3 | 2.1 | 3 | 16–250 | 0.9997 | y = 0.47x + 0.09 | 3.80 | 11.53 | 16 | 2.9 | 101.9 ± 3.0 | 2.4 | 101.4 ± 2.5 | 120 | 96 | 102.1 | 3.7 | 63 | 0.0 | 100.0 ± 0.0 | 2.7 | 102.7 ± 2.8 | 120 | 99.8 | 3.3 | 250 | 0.8 | 101.7 ± 0.8 | 2.3 | 101.4 ± 2.3 | 144 | 101.8 | 1.0 | 4 | 16–250 | 0.9992 | y = 0.77x + 0.07 | 0.37 | 1.24 | 16 | 0.0 | 100.0 ± 0.0 | 3.2 | 109.2 ± 3.4 | 45 | 36 | 100.0 | 0.9 | 63 | 1.1 | 100.7 ± 1.1 | 2.9 | 99.6 ± 2.9 | 45 | 100.0 | 1.7 | 250 | 0.3 | 99.8 ± 0.3 | 1.2 | 100.3 ± 1.2 | 54 | 99.5 | 1.2 | 5 | 8–250 | 0.9995 | y = 4.69x + 8.50 | 0.64 | 1.95 | 16 | 1.4 | 99.2 ± 1.3 | 1.9 | 100.5 ± 2.0 | 11 | 9 | 101.0 | 3.9 | 63 | 1.2 | 100.5 ± 1.2 | 1.5 | 98.9 ± 1.5 | 11 | 103.3 | 0.9 | 250 | 0.2 | 99.8 ± 0.2 | 1.3 | 99.5 ± 1.3 | 13 | 102.7 | 0.7 | 6 | 16–250 | 0.9996 | y = 1.36x + 1.08 | 1.96 | 6.55 | 16 | 0.0 | 100.0 ± 0.0 | 1.9 | 103.5 ± 1.9 | 49 | 39 | 102.6 | 3.6 | 63 | 0.6 | 100.4 ± 0.6 | 1.7 | 99.1 ± 1.7 | 49 | 103.0 | 0.9 | 250 | 0.3 | 99.7 ± 0.3 | 0.3 | 99.8 ± 0.3 | 59 | 102.6 | 1.7 | 7 | 16–250 | 0.9996 | y = 1.19x + 0.94 | 0.61 | 2.04 | 16 | 0.0 | 100.0 ± 0.0 | 2.7 | 103.0 ± 2.7 | 21 | 17 | 101.6 | 3.2 | 63 | 0.7 | 100.4 ± 0.7 | 2.8 | 99.8 ± 2.7 | 21 | 101.1 | 1.6 | 250 | 0.2 | 99.9 ± 1.2 | 1.7 | 100.6 ± 1.8 | 25 | 100.4 | 1.3 | 8 | 16–250 | 0.9995 | y = 1.04x + 0.75 | 1.60 | 5.33 | 16 | 0.0 | 100.0 ± 0.0 | 3.0 | 102.2 ± 3.0 | 106 | 85 | 104.3 | 5.2 | 63 | 0.8 | 100.5 ± 0.8 | 2.2 | 99.4 ± 2.2 | 106 | 100.2 | 2.9 | 250 | 0.2 | 99.9 ± 0.2 | 1.4 | 99.6 ± 1.4 | 127 | 102.3 | 1.3 | 9 | 16–250 | 0.9997 | y = 9.13x + 13.67 | 3.85 | 12.82 | 16 | 3.9 | 96.7 ± 3.8 | 0.3 | 99.6 ± 0.3 | 40 | 32 | 101.6 | 2.6 | 63 | 1.1 | 100.2 ± 1.1 | 0.3 | 99.7 ± 0.3 | 40 | 100.6 | 2.9 | 250 | 0.1 | 99.9 ± 0.1 | 0.2 | 99.8 ± 0.2 | 48 | 103.2 | 1.7 |
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