Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry

Table 1

Mass parameters for the 8 endogenous alkaloids.

Compound name	Formula	Adduct/charge	Theoretical mass (m/z)	Experimental mass (m/z)	Mass error (×10^−6)	Retention time (min)
Nicotine	C10H14N2	[M + H]^+	163.123	163.1231	0.9	4.69
Anabasine	C10H12N2	[M + H]^+	161.107	161.1069	−2.5	2.97
Choline	C5H14NO^+	[M + H]^+	104.107	104.1071	1.5	4.30
Tryptamine	C10H12N2	[M + H]^+	161.107	161.1075	0.9	1.80
Putrescine	C4H12N2	[M + H]^+	89.107	89.1073	−0.1	6.55
Nicotinamide	C6H6N2O	[M + H]^+	123.055	123.0554	0.8	1.04
Muscarine	C9H20NO2^+	[M + H]^+	174.149	174.1491	0.6	3.79
Cotinine	C10H12N2O	[M + H]^+	177.102	177.1025	1.4	1.34