Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry

Table 2

Linearity, LODs, and LOQs of the 8 endogenous alkaloids.

Compound	Linear equation	Linear range (μg/L)	R^2	LOD (mg/kg)	LOQ (mg/kg)
Nicotine	Y = 10091.33111X + 3329.87164	0.5–500	0.99866	0.005	0.010
Anabasine	Y = 11037.04373X + 23487.04699	0.2–500	0.99844	0.002	0.004
Choline	Y = 14658.46420X − 5.77788 × 10^4	0.5–500	0.99785	0.005	0.010
Tryptamine	Y = 946.35326X + 3594.74788	5.0–500	0.99876	0.050	0.100
Putrescine	Y = 483.76213X + 2103.10951	10.0–500	0.99876	0.100	0.200
Nicotinamide	Y = 2672.35197X − 3753.02170	0.5–500	0.99899	0.005	0.010
Muscarine	Y = 28863.69968X − 3.26291 × 10^5	0.2–500	0.99932	0.002	0.004
Cotinine	Y = 6622.74425X + 3.13607 × 10^4	0.5–500	0.99928	0.005	0.010