Determination of 8 Endogenous Alkaloid Components in Boletus Using Ultrahigh-Performance Liquid Chromatography Combined with Quadrupole-Time of Flight Mass Spectrometry

Table 3

Linearity, LODs, and LOQs of the 8 endogenous alkaloids.

Compound	Background (mg/kg)	Added (mg/kg)	Recovery (%)	RSD (%)
Nicotine	0.160	0.010	87.6	1.93
		0.030	85.2	3.90
		0.100	90.4	1.62
Anabasine	0.111	0.004	101.5	4.15
		0.012	85.9	0.30
		0.040	81.1	1.63
Choline	301	0.010	89.2	1.45
		0.030	92.3	0.44
		0.100	88.7	0.13
Tryptamine	ND	0.100	83.3	2.10
		0.300	82.4	6.82
		1.00	82.3	1.89
Putrescine	ND	0.200	80.1	7.59
		0.600	89.2	3.08
		2.00	88.3	4.21
Nicotinamide	7.01	0.010	82.5	3.38
		0.030	80.1	2.58
		0.100	91.7	1.43
Muscarine	ND	0.004	97.5	3.45
		0.012	91.3	1.68
		0.040	88.8	0.06
Cotinine	ND	0.010	85.4	4.54
		0.030	87.3	7.58
		0.100	90.5	2.19

ND means not detected.