A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco

Table 2

Repeatability and reproducibility of the pGMT27 plasmid as a calibrator in qPCR.

Targets	True copy number	Ct repeatability (one day)				SD	RSD (%)	True copy number	Ct reproducibility (different days)				SD	RSD (%)
		1	2	3	Mean				1	2	3	Mean
P-35S	100	32.96	33.68	32.94	33.19	0.42	1.28	100	33.24	32.90	33.26	33.14	0.20	0.62
	1,000	30.21	30.37	30.33	30.31	0.08	0.27	1,000	30.66	30.47	30.41	30.51	0.13	0.43
	10,000	27.10	27.22	27.07	27.13	0.08	0.29	10,000	27.38	27.33	27.11	27.27	0.14	0.52
	100,000	23.60	23.41	23.86	23.62	0.23	0.97	100,000	23.71	23.77	23.54	23.67	0.12	0.50
	1,000,000	20.07	20.14	20.07	20.09	0.04	0.18	1,000,000	20.20	20.14	20.13	20.16	0.04	0.18
NR	100	32.16	32.99	32.65	32.60	0.42	1.29	100	33.66	33.72	34.35	33.91	0.39	1.14
	1,000	29.42	29.25	28.99	29.22	0.22	0.74	1,000	29.99	29.88	30.04	29.97	0.08	0.27
	10,000	26.20	26.00	25.80	26.00	0.20	0.76	10,000	26.77	26.59	26.82	26.73	0.12	0.46
	100,000	22.61	22.54	22.72	22.63	0.09	0.41	100,000	23.05	22.93	23.12	23.03	0.09	0.40
	1,000,000	19.21	19.18	18.77	19.05	0.25	1.29	1,000,000	19.70	19.57	19.59	19.62	0.07	0.35