Characterization of Lactic Bacteria Isolated from Raw Milk and Their Antibacterial Activity against Bacteria as the Cause of Clinical Bovine Mastitis
Table 4
Enzymatic activity detected using the APIZYM system of selected LAB strains.
Enzymes
Strains
ILT1
ILT2
ILT3
ILT4
ILT5
ILT6
ILT7
Alkaline phosphate
0
0
0
0
0
0
0
Esterase (C4)
5
5
5
0
5
5
0
Esterase lipase (C8)
0
5
5
5
5
10
5
Lipase (C14)
0
5
5
5
5
5
5
Leucine arylamidase
0
0
0
0
0
0
0
Valine arylamidase
5
>40
30
5
10
>40
10
Cystine arylamidase
5
>40
20
10
5
>40
10
Trypsin
0
5
0
0
0
0
0
ᾳ-Chymotrypsin
0
0
0
0
0
0
0
Acid phosphatase
0
0
0
0
0
0
0
Naphthol-phosphohydrolase
0
20
0
0
0
0
0
ᾳ-Galactosidase
5
0
0
5
5
5
0
β-Galactosidase
0
0
0
0
0
0
0
Β-Glucuronidase
5
30
0
5
0
5
0
Glucosidase
0
0
0
0
0
0
0
Β-Glucosidase
0
0
0
5
0
10
0
N-Acetyl-β-glucosaminidase
10
10
>40
>40
5
30
5
ᾳ-Mannosidase
5
>40
20
20
5
>20
5
ᾳ-Fucosidase
0
0
0
0
0
0
0
Enzymatic activity (approximate values) expressed as free nanomole hydrolyzed substrate was obtained from the color intensity. 0: no activity; 1: liberation/released of 5 nmol; 2 : 10 nmol; 3 : 20 nmol; 4 : 30 nmol; and 5: >40 nmol.
