Metabolomic Biomarkers Differentiate Soy Sauce Freshness under Conditions of Accelerated Storage
Table 3
Average peak heights (log 2 converted) of analytes resolved from aged soy sauce samples selected on the basis that their relative concentrations changed as a result of aging.
Feature ID/sample
Average peak height (log2)
value relative to 0 month treatment
0 months
2 months
4 months
6 months
8 months
2 months
4 months
6 months
8 months
Aconitic acid 3TMS
22.682
22.688
22.659
22.645
22.603
0.758
0.415
0.196
0.018
Arabinose 4TMS 1MOX
29.813
29.743
29.622
29.573
29.456
0.178
0.011
0.007
0.001
Glyceric acid 3TMS
23.978
24.013
24.026
24.071
24.031
0.125
0.091
0.008
0.042
Glycolic acid 2TMS
23.467
23.500
23.532
23.576
23.537
0.071
0.009
0.001
0.005
Glycylglycine 4TMS
26.950
26.949
26.953
26.934
26.900
0.941
0.887
0.377
0.019
Hydroxyproline 3TMS
24.003
24.028
24.042
24.076
24.038
0.343
0.205
0.045
0.209
L-Tryptophan 3TMS
26.401
26.205
26.039
25.897
25.761
0.020
0.002
0.001
≤≤0.001
Sugar RT 10.763
27.066
26.914
26.711
26.553
26.433
0.167
0.004
0.002
≤0.001
Sugar RT 10.884
27.991
27.858
27.660
27.591
27.465
0.033
0.002
0.001
≤0.001
Sugar RT 11.176
25.703
25.610
25.504
25.460
25.362
0.027
0.002
0.002
≤0.001
Sugar RT 11.635
24.734
24.536
24.286
24.097
24.003
0.167
0.004
0.002
≤0.001
Sugar RT 11.94
28.916
28.928
28.923
28.960
28.911
0.493
0.634
0.042
0.740
Sugar RT 12.287
25.094
24.884
24.655
24.474
24.382
0.142
0.004
0.002
≤0.001
Sugar RT 13.44
28.475
28.368
28.232
28.104
28.003
0.380
0.041
0.040
0.001
Sugar RT 13.594
25.911
25.955
26.003
26.058
26.048
0.232
0.050
0.017
0.004
Sugar RT 13.824
30.200
30.191
30.152
30.147
30.058
0.796
0.285
0.267
0.010
Sugar RT 13.847
30.172
30.075
29.950
29.868
29.758
0.432
0.049
0.079
0.003
Sugar RT 13.966
29.289
29.183
29.037
28.905
28.798
0.437
0.051
0.049
0.001
Sugar RT 14.867
30.611
30.513
30.384
30.294
30.186
0.425
0.049
0.084
0.003
Sugar RT 15.414
26.738
26.728
26.692
26.712
26.639
0.806
0.236
0.510
0.026
Sugar RT 15.75
23.527
23.474
23.411
23.401
23.300
0.199
0.023
0.014
0.002
Sugar RT 19.354
23.797
23.857
23.936
24.007
24.001
0.206
0.003
0.003
0.001
Sugar RT 19.525
22.587
22.655
22.711
22.799
22.790
0.185
0.002
0.002
0.001
Sugar RT 20.37
24.714
24.633
24.535
24.420
24.338
0.487
0.113
0.073
0.003
Sugar RT 21.555
25.564
25.532
25.482
25.456
25.380
0.567
0.183
0.181
0.015
Sugar RT 21.798
22.691
22.663
22.659
22.616
22.543
0.624
0.440
0.095
0.017
Unknown RI1426 m/z 189.112
20.615
20.685
20.714
20.785
20.780
0.007
0.004
≤0.001
≤0.001
Unknown RI 1428 m/z 186.131
22.611
22.585
22.578
22.594
22.565
0.043
0.015
0.102
0.020
Unknown RI 1448 m/z 218.115
20.486
20.248
20.083
19.797
19.772
0.321
0.049
0.026
0.003
Unknown RI 1465 m/z 232.118
23.360
23.308
23.274
23.242
23.188
0.026
≤0.001
0.019
≤0.001
Unknown RI 1475 m/z 156.084
18.805
18.732
18.718
18.761
18.686
0.013
0.001
0.063
0.016
Unknown RI 1490 m/z 144.084
23.012
22.949
22.859
22.804
22.693
0.090
0.009
0.003
≤0.001
Unknown RI 1545 m/z 123.044.
21.027
21.116
21.216
21.340
21.379
0.002
≤0.001
≤0.001
≤0.001
Unknown RI 1554 m/z 292.134
21.464
21.508
21.526
21.569
21.529
0.132
0.059
0.021
0.036
Unknown RI 1563 m/z 345.137
21.223
20.905
20.873
20.896
20.836
≤0.001
≤0.001
≤0.001
≤0.001
Unknown RI 1576 m/z 142.105
23.665
23.635
23.569
23.565
23.455
0.536
0.027
0.071
0.002
Unknown RI 1596 m/z 231.087
20.565
20.585
20.613
20.670
20.634
0.434
0.115
0.009
0.034
Unknown RI 1643 m/z 245.102
26.367
26.436
26.483
26.566
26.554
0.033
0.006
0.001
0.002
Unknown RI 1664 m/z 204.1
23.365
23.303
23.229
23.170
23.129
0.466
0.081
0.071
0.003
Unknown RI 1671 m/z 173.087
22.758
22.728
22.658
22.637
22.540
0.419
0.051
0.031
0.002
Unknown RI 1674 m/z 214.126
21.175
21.159
21.125
21.163
21.104
0.571
0.042
0.639
0.011
Unknown RI 1691 m/z 129.037
22.474
22.477
22.427
22.410
22.357
0.894
0.141
0.081
0.008
Unknown RI 1693 m/z 275.161
21.823
21.872
21.897
21.954
21.929
0.140
0.060
0.033
0.011
Unknown RI 1769 m/z 129.073
22.545
22.592
22.654
22.731
22.758
0.029
≤0.001
≤0.001
≤0.001
Unknown RI 1769 m/z 286.122
21.364
21.369
21.407
21.447
21.491
0.901
0.163
0.054
0.014
Unknown RI 1773 m/z 225.105
21.137
21.148
21.169
21.206
21.198
0.677
0.207
0.059
0.042
Unknown RI 1775 m/z 129.037
21.828
21.875
21.894
21.940
21.910
0.069
0.051
0.006
0.014
Unknown RI 1831 m/z 362.163
20.409
20.407
20.380
20.379
20.307
0.942
0.339
0.330
0.017
Unknown RI 1838 m/z 217.107
24.666
24.592
24.463
24.388
24.276
0.688
0.156
0.190
0.011
Unknown RI 1846 m/z 245.102
25.195
25.276
25.354
25.446
25.462
0.015
0.002
≤0.001
≤0.001
Unknown RI 1855 m/z 245.102
27.646
27.732
27.810
27.912
27.908
0.020
0.003
≤0.001
≤0.001
Unknown RI 1873 m/z 226.113
23.724
23.760
23.797
23.855
23.839
0.238
0.029
0.004
0.006
Unknown RI 1876 m/z 307.157
22.276
22.338
22.375
22.428
22.406
0.072
0.024
0.020
0.003
Unknown RI 1881 m/z 157.068
24.998
25.050
25.091
25.162
25.148
0.142
0.043
0.009
0.003
Unknown RI 1937 m/z 142.105
22.673
22.663
22.658
22.656
22.622
0.492
0.156
0.501
0.008
Unknown RI 1941 m/z 70.065
22.556
22.587
22.606
22.632
22.614
0.249
0.062
0.013
0.022
Unknown RI 1955 m/z 143.052
21.127
21.104
21.082
21.090
21.042
0.383
0.086
0.202
0.014
Unknown RI 1961 m/z 333.137
21.955
22.006
22.053
22.123
22.097
0.089
0.019
0.002
0.004
Unknown RI 1961 m/z 387.158
21.043
21.091
21.142
21.218
21.216
0.097
0.002
0.002
0.001
Unknown RI 2013 m/z 174.113
22.964
23.008
23.033
23.092
23.061
0.263
0.074
0.043
0.015
Unknown RI 2026 m/z 188.128
23.381
23.243
23.179
23.193
23.140
0.060
0.003
0.010
0.001
Unknown RI 2036 m/z 311.124
21.072
21.114
21.190
21.252
21.261
0.279
0.003
≤0.001
0.002
Unknown RI 2052 m/z 174.113
25.791
25.787
25.753
25.751
25.675
0.924
0.387
0.360
0.049
Unknown RI 2069 m/z 224.074
20.424
20.302
20.240
20.180
20.037
0.241
0.128
0.040
0.021
Unknown RI 2082 m/z 238.113
21.850
21.880
21.920
21.987
21.956
0.619
0.233
0.012
0.123
Unknown RI 2127 m/z 142.068
21.357
21.255
21.226
21.245
21.142
0.087
0.052
0.015
0.021
Unknown RI 2144 m/z 326.19
23.523
23.191
23.138
23.153
23.068
0.030
0.016
0.023
0.008
Unknown RI 2147 m/z 475.229
21.205
21.008
20.898
20.890
20.803
0.026
0.003
0.007
0.001
Unknown RI 2158 m/z 232.118
22.864
22.773
22.709
22.643
22.487
0.217
0.093
0.026
0.015
Unknown RI 2219 m/z 142.068
21.682
21.553
21.558
21.545
21.491
0.104
0.107
0.022
0.024
Unknown RI 2252 m/z 217.107
24.622
24.662
24.703
24.737
24.701
0.246
0.042
0.017
0.060
Unknown RI 2256 m/z 160.079
21.564
21.646
21.716
21.819
21.777
0.230
0.002
0.002
0.001
Unknown RI 2262 m/z 255.134
21.612
21.708
21.787
21.913
21.899
0.148
0.001
0.004
≤0.001
Unknown RI 2280 m/z 232.118
24.412
24.469
24.522
24.551
24.501
0.196
0.049
0.039
0.063
Unknown RI 2308 m/z 269.15
21.981
22.075
22.163
22.301
22.298
0.108
0.001
0.003
≤0.001
Unknown RI 2310 m/z 387.213
22.158
22.272
22.340
22.409
22.390
0.042
0.002
0.004
0.001
Unknown RI 2323 m/z 116.089
19.736
19.845
19.925
20.020
19.999
0.109
0.004
0.002
0.001
Unknown RI 2326 m/z 343.15
21.586
21.724
21.817
21.868
21.868
0.143
0.002
0.001
0.001
Unknown RI 2356 m/z 142.068
20.013
20.081
20.130
20.188
20.145
0.216
0.002
0.016
0.006
Unknown RI 2381 m/z 84.081
22.358
22.383
22.419
22.521
22.495
0.785
0.190
0.085
0.006
Unknown RI 2385 m/z 174.113
24.701
24.704
24.700
24.674
24.656
0.687
0.941
0.202
0.011
Unknown RI 2446 m/z 98.096
20.979
20.987
21.036
21.155
21.141
0.929
0.077
0.069
0.014
Unknown RI 2454 ma 327.131
22.215
22.255
22.351
22.390
22.422
0.496
0.121
0.165
0.027
Unknown RI 2470 m/z 217.107
23.859
23.950
24.071
24.153
24.157
0.341
0.014
0.007
0.001
Unknown RI 2477 m/z 204.1
23.443
23.529
23.630
23.725
23.773
0.269
0.033
0.007
0.002
Unknown RI 2489 m/z 217.107
23.387
23.530
23.687
23.804
23.841
0.197
0.004
0.002
≤0.001
Unknown RI 2493 m/z 156.084
25.002
25.151
25.302
25.411
25.439
0.298
0.005
0.004
≤0.001
Unknown RI 2525 m/z 417.182
20.587
20.621
20.711
20.820
20.791
0.577
0.101
0.034
0.018
Unknown RI 2635 m/z 132.081
21.575
21.645
21.743
21.811
21.764
0.411
0.013
0.006
0.006
Unknown RI 2652 m/z 132.081
20.145
20.274
20.357
20.448
20.365
0.200
0.035
0.009
0.027
Unknown RI 2758 m/z 191.092
23.313
23.268
23.232
23.190
23.118
0.315
0.140
0.065
0.010
Unknown RI 2940 m/z 174.113
20.482
20.582
20.663
20.782
20.738
0.212
≤0.001
0.007
0.001
Unknown RI1602 m/z 200.11
21.879
21.870
21.854
21.892
21.813
0.677
0.213
0.477
0.010
value columns depict the result of a statistical comparison with unaged soy sauce sample. Bold values denote values less than 0.05. For each “unknown” peak, RI represents a relative retention index. TMS: trimethylsiloxane; MOX: methoxamine.
