Comparison of Synergistic Effect of Nisin and Monolaurin on the Inactivation of Three Heat Resistant Spores Studied by Design of Experiments in Milk

Table 2

Different responses and their correlation coefficients obtained in the inactivation of Bacillus spores by nisin and monolaurin in two different media.

Medium	Strain	Equation (Y = log N0/N1)	R^2 (correlation coefficient)
Distilled water	Paenibacillus sp.	Y1 = 28.362–0.012x1 − 0.034x2 − 6.398x3 + 0.000x1^2 − x1x2 + 0.004x1x3 + 0.000x2^2 + 0.006x2x3 + 0.301x3^2	0.98
	Terribacillus aidingensis	Y2 = −3.938 + 0.006x1 + 0.051x2 − 0.451x3 + 0.000x1^2 − 0.000x1x2 + 0.003x1x3 − 0.000x2^2 − 0.003x2x3 + 0.074x3^2	0.95
	Bacillus sporothermodurans	Y3 = −3.795 + 0.014x1 + 0.039x2 − 0.153x3 − 0.000x1^2 + 0.000x1x2 − 0.000x1x3 − 0.000x2^2 − 0.003x2x3 + 0.085x3^2	0.99
UHT milk	Paenibacillus sp.	Y1’’ = 29.346 − 0.018x1 − 0.039x2 − 6.389x3 + 0.000x1^2 − 0.000x1x2 + 0.004x1x3 − 0.000x2^2 + 0.008x2x3 + 0.266x3^2	0.99
	Terribacillus aidingensis	Y2’’ = −3.659 + 0.001x1 + 0.033x2 + 0.259x3 − 0.000x1^2 + 0.000x1x2 − 0.000x1x3 − 0.000x2^2 − 0.000x2x3 − 0.021x3^2	0.96
	Bacillus sporothermodurans	Y3’’ = −0.947 + 0.020x1 + 0.031x2 − 0.722x3 − 0.000x1^2 − 5.E-7x1x2 − 0.001x1 x3 − 0.000x2^2 − 0.002x2x3 + 0.108x3^2	0.98