Journal of Immunology Research

Journal of Immunology Research / 2017 / Article
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Genetic Factors of Autoimmune Diseases 2017

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Volume 2017 |Article ID 3073504 |

Satoru Joshita, Takeji Umemura, Eiji Tanaka, Masao Ota, "Genetic Contribution to the Pathogenesis of Primary Biliary Cholangitis", Journal of Immunology Research, vol. 2017, Article ID 3073504, 6 pages, 2017.

Genetic Contribution to the Pathogenesis of Primary Biliary Cholangitis

Academic Editor: Nancy Agmon-Levin
Received18 Oct 2016
Accepted12 Jan 2017
Published01 Feb 2017


Formerly termed primary biliary cirrhosis, primary biliary cholangitis (PBC) is a chronic and progressive cholestatic liver disease characterized by the presence of antimitochondrial antibodies. Ursodeoxycholic acid (UDCA) therapy is the most effective and approved treatment for PBC and leads to a favorable outcome in the vast majority of cases. Although the etiology of PBC has not yet been elucidated, human leukocyte antigen (HLA) class II alleles have been consistently associated with disease onset for decades. Individuals in different geographic regions of the world may have varying susceptibility alleles that reflect indigenous triggering antigens. In this review, we describe the influence of HLA alleles and other gene polymorphisms on PBC along with the results of genome-wide association studies (GWAS) on this disease.

1. Introduction

Primary biliary cholangitis (PBC), formerly known as primary biliary cirrhosis [1, 2], is a liver-specific autoimmune disease characterized by female preponderance and destruction of intrahepatic bile ducts that often results in cirrhosis and hepatic failure [35]. The prevalence of PBC ranges from 20 to 40 cases per 100,000 persons [46], although the number of patients with PBC, specifically asymptomatic PBC, is on the rise due mainly to increased awareness and earlier detection by disease-specific antimitochondrial antibodies (AMAs). Ursodeoxycholic acid (UDCA) therapy is the most effective treatment for PBC and is recommended by most guidelines [7, 8]. The vast majority of patients with PBC show a favorable response to UDCA treatment despite some cases of disease progression via unknown mechanisms [9, 10]. Genetic factors are considered to play a prominent role in disease onset as higher concordance rates in monozygotic twins than in dizygotic twins and familial clustering of patients with PBC has been demonstrated in family and population studies [1116]. However, the etiology of this disease has yet to be conclusively clarified; PBC is presumed to be a multifactorial polygenic condition caused by allelic triggers and environmental factors in genetically susceptible individuals, although epigenetic mechanisms, such as instability of X chromosome gene expression, may also participate in the disease’s female predominance [1719].

In the present article, we summarize the literature on human leukocyte antigen (HLA) involvement in PBC onset and GWAS findings from North American, European, and Japanese populations to explore the disease pathways of PBC pathogenesis.

2. Associations between HLA and PBC Susceptibility

Many significant susceptibility single nucleotide polymorphisms (SNPs), such as CTLA4, TNF-a, STAT4, PTPN22, and VDR, have been identified using candidate gene methods [2024]. Among them, however, only HLA has consistently been associated with PBC in distinct patient cohorts across ethnicities.

Located on the most gene-dense genomic region on chromosomal position 6p21 [25], HLA genes are extremely polymorphic and play an essential role in numerous biologically and medically relevant processes. The products of the classical HLA class I (A, B, and C) and class II (DR, DQ, and DP) genes include cell-surface glycoproteins involved in the binding and presentation of self- or non-self-peptides to T-cell receptors (TCRs). Class I molecules present endogenous peptides derived from viruses to CD8+ cytotoxic T cells, while class II molecules present processed peptides from exogenous pathogens to CD4+ helper T cells. The extent of endogenous and exogenous peptide binding to HLA molecules depends on allelic polymorphisms. Additionally, both HLA class I and II molecules have functional roles in protein interactions, transcription regulation involved in the inflammatory response, and natural killer cell-cytokine interactions as part of innate immunity.

HLA polymorphisms have been extensively studied in immune-mediated diseases, revealing associations of particular alleles with ankylosing spondylitis (AS), Behçet’s disease (BD), psoriasis, multiple sclerosis (MS), insulin-dependent diabetes mellitus (IDDM), systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), rheumatoid arthritis (RA), narcolepsy, autoimmune hepatitis (AIH), and autoimmune pancreatitis (AIP) among others. Early investigations on associations between HLA polymorphisms and PBC were carried out more than a quarter-century ago [37]. Based on these findings, subsequent cumulative studies have provided evidence that PBC is associated with DRB108 as predisposing and DRB111 and DRB113 as protective alleles [28, 38]. Li et al. conducted a meta-analysis to assess for relationships between HLA class II and disease susceptibility to PBC and demonstrated that HLA DR07 and 08 alleles were risk factors for PBC in certain populations, whereas DR11, 12, 13, and 15 alleles were protective factors [39].

Several key reports [2628] on the association between HLA haplotype and PBC susceptibility or resistance are summarized in Table 1. HLA DR08 alleles caused disease susceptibility, while HLA DRB113 and 11 alleles conferred disease protection in haplotype analyses across ethnicities. Both protective DRB111 and DRB113 alleles have also been implicated DRB111 against hepatitis C [40], human papilloma [41], and human immunodeficiency [42] and DRB113 against hepatitis C [43], human papilloma [44], and human immunodeficiency [45] viruses along with malaria [44]. Thus, one of the pathogenic mechanisms in PBC may be bacterial infection as these protective HLA class II alleles play a functional role in blocking the invasion of infectious agents.

StudyPopulationHLA allele valueOR (95% CI)


Umemura et al. [26]JapaneseDRB108:03-DQB106:010.0000252.22 (1.53–3.20)
DRB104:05-DQB104:010.0441.38 (10.2–1.87)
Zhao et al. [27] ChineseDRB108:03-DQB106:01<0.00013.17 (1.91–5.23)
DRB107:01-DQB102:020.0051.85 (1.20–2.83)
Donaldson et al. [28] UKDRB108:01-DQA10401-DQB104:020.00272.9


Umemura et al. [26] JapaneseDRB113:02-DQB106:040.000930.27 (0.12–0.60)
DRB111:01-DQB103:010.030.37 (0.15–0.88)
Zhao et al. [27]ChineseDRB112:02-DQB103:010.0150.43 (0.22–0.86)
Donaldson et al. [28] UKDRB111:01-DQA105:01-DQB103:010.0860.47

However, individuals harboring the above haplotypes constitute only a minority of patients with PBC, suggesting that other candidate genes and environmental cues evoke PBC pathogenesis. Umemura et al. [26] reported the possibility that the distribution of DRB1 amino acid residues encoded by different HLA DRB1 alleles influenced the binding affinity to antigens, which might also be a predominant factor in PBC susceptibility.

3. GWAS on PBC

There have been extensive GWAS in patients with PBC, a number of which documenting significant associations with disease risk. To date, five GWAS [2933], two Illumina immunochip studies [34, 35], and one genome-wide meta-analysis (GWMA) [36] on PBC have been performed on well-characterized cohorts in North American, European, and Japanese populations (Table 2). These investigations clarified that the HLA class II domain possessed the strongest association with disease susceptibility, particularly at the HLA-DRB1, HLA-DQA1, and HLA-DQB1 loci. However, HLA alone does not explain the entire genetic predisposition to PBC, mainly since 80–90% of patients with the disease do not carry the most common HLA susceptibility alleles. In this regard, other genes apart from HLA loci are suggested to contribute to disease development. At present, GWAS have identified 39 non-HLA loci predisposing to PBC at a genome-wide level of significance (Table 3).


Hirschfield et al. [29]2009Illumina HumanHap 3701,0312,713
Hirschfield et al. [30]2010Illumina HumanHap 3701,3514,700
Liu et al. [31]2010Illumina 610K9454,651
Mells et al. [32]2011Illumina 660W-Quad1,8405,163
Nakamura et al. [33]2012Affymetrix Axiom1,2741,091
Juran et al. [34]2012Immunochip2,4265,731
Liu et al. [35]2012Immunochip2,8618,514
Cordell et al. [36]2015GWMA2,76410,475

SNPOR valueCandidate gene(s)Disease(s) with shared risk loci


22q12[36]rs127121331.14IL1RL1, IL1RL2,
2q12[34]rs101867461.21IL1RL1, IL1RL2,
2q32[32]rs109314681.50STAT4, STAT1RA, SLE, Sjögren’s, IBD, SSc, BD

3q13[35]rs22933701.39CD80MS, SLE, Celiac


55p13[35]rs68717481.30IL7RMS, UC
5q33[36]rs25468901.15IL12B, LOC285626

66q23[36]rs69334041.18OLIG3, TNFAIP3
6q23[34]rs69202201.29OLIG3, TNFAIP3

77p14[32]rs69744911.25ELMO1RA, Celiac
7q32[35]rs351882611.52IRF5RA, SLE, SSc, UC

88q24[34]rs26080291.23PVT1, GSDMC

99p32[33]rs49794621.57TNFSF15UC, CD

11q23[35]rs800651071.39CXCR5, DDX6RA, IBD, Celiac

1212p13[35]rs18006931.27TNFRSF1A, LTBRMS
12q24[35]rs110659791.20SH2B3RA, T1DM, Hyperthyroidism, Celiac
12q24[34]rs73093251.26SH2B3RA, T1DM, Hyperthyroidism, Celiac

1313q14[34, 35]rs38627381.33TNFSF11CD


1616p13[35]rs127087151.29CLEC16A, SOCS1MS, UC, T1DM
16q24[32]rs111174321.31IRF8MS, IBD, RA, SSc

1717q12[35]rs175648291.26IKZF3UC, CD, RA, T1DM

1919p12[35]rs345364431.91TYK2IBD, RA, SLE, psoriasis, T1DM


CD, Crohn’s disease; UC, ulcerative colitis; T1DM, type 1 diabetes mellitus.

The first GWAS [29] in a North American cohort identified a significant association of PBC with genetic variants at IL12A, encoding IL-12 p35, and IL12RB2, encoding IL-12 receptor β2. Modest genome-wide associations with disease risk for SNPs at the signal transducer and activator of transcription 4 (STAT4) and cytotoxic T-lymphocyte-associated protein 4 (CTLA4) loci were found as well. The second GWAS [31] confirmed the existence of additional risk loci, including interferon regulatory factor 5 (IRF5), transportin 3 (TNPO3), and SPIB encoding a transcription factor involved in B-cell receptor signaling and T-cell lineage decisions. A subsequent noteworthy GWAS from Japan showed that the IL12A and IL12RB2 loci were not significantly associated with PBC, but rather that the TNFSF15 and POU2AF1 genes constituted novel risk loci in Japanese patients with PBC along with other non-HLA loci, including IL7R, IKZF3, CD80, STAT4, and NFKB1. This discrepancy among ethnicities indicated important differences in the pathogenesis of PBC despite several common key molecules and pathways, such as the IL-12 pathway to induce Th1 polarization of CD4+ T cells. Our body of evidence suggests that there may be an inherited abnormality in immune regulation during PBC onset and perhaps an inability to suppress inflammatory attacks on small bile ducts once initiated.

It should be noted that Juran et al. identified risk-conferring epistatic interactions between IL12RB2 and IRF5 loci [34] as well as between CTLA4 and TNFα loci in the pre-GWAS era [46]. Epistatic interactions between genes revealed by GWAS in the pathogenesis of PBC should be explored in future studies.

While gene associations are of considerable interest in the pathogenesis of PBC, virtually none have been translated into useful clinical testing. For instance, the importance of the IL-12 pathway in PBC onset has been highlighted in animal models and in the case of a child with a congenital IL-12 deficiency who developed PBC [47]. Although antibodies or drugs targeting the IL-12 pathway would seem to be effective, clinical trials using ustekinumab, a human monoclonal antibody directed against IL-12 and IL-23, have failed to produce effects in phase II trials [48]. One reason explaining the discrepancy between GWAS results and clinical testing may be that clinicians typically encounter patients who have already become complicated with cholestasis; in fact, the immunological destruction of cholangiocytes occurs in the very early stages of PBC. Thus, the mechanisms of disease progression should also be addressed to halt the deterioration of disease status and afford PBC patients an improved prognosis.

Lastly, it is particularly interesting that many genes implicated in PBC pathogenesis by GWAS have also been reported in other autoimmune diseases, such as SLE, systemic sclerosis (SSc), and Sjögren’s syndrome (Table 3), suggesting a genetic overlap. Understanding the mechanisms involved in the onset and progression of certain autoimmune diseases may accordingly shed light on those in PBC.

4. Conclusions and Future Directions

The pathogenesis of PBC is incompletely understood but appears to involve genetic susceptibility and resistance alleles in HLA and other gene loci, with a possible overlap with several autoimmune diseases. It is also probable that genetically susceptible individuals develop PBC following environmental cues, leading to both adaptive and innate immune responses that result in portal inflammation and bile duct epithelial damage. In addition to susceptibility, the precise mechanisms of PBC progression should be addressed to improve patient prognosis and quality of life.

Competing Interests

The authors declare that they have nothing to disclose regarding funding from industries or conflict of interests with respect to this manuscript.


The authors sincerely appreciate the financial support provided by Grants-in-Aid for Young Scientists (Kakenhi 16K21069) and the Promotion Project of Education, Research, and Medical Care from Shinshu University Hospital. They also thank Mr. Trevor Ralph for his English editorial assistance.


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