Mini-αA Upregulates the miR-155-5p Target Gene CDK2 and Plays an Antiapoptotic Role in Retinal Pigment Epithelial Cells during Oxidative Stress

Figure 4

miR-155-5p participates in the therapeutic effects of mini-αA by regulating CDK2. ARPE-19 cells were treated with NaIO₃ following which interference and overexpression of miR-155-5p were performed. (a) Four target genes related to the cell cycle predicted by miRanda were detected by qRT-PCR. (b) The expression of Bcl2, Bax, cleaved caspase-3, and CDK2 was measured by western blotting. (c) Densitometric quantitation of proteins is probed in Figure 4(b). (d) The binding site between 3′UTR of CDK2 and miR-155-5p was predicted by miRanda, and dual-luciferase reporter assay was performed to detect the luciferase activity in wild-type (WT) and mutant (mut) of 293T cells after transfection with NC mimics and miR-155-5p mimics. ns, not significant; and .

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