Inducible Lentivirus-Mediated siRNA against TLR4 Reduces Nociception in a Rat Model of Bone Cancer Pain

Figure 2

mRNA expression of TLR4 detected by real-time PCR. As shown in Figure 2, bone cancer in legs increased the TLR4 mRNA expression in the spinal cord in the five groups receiving Walker 256 cells injection. On the 3rd, 7th, 14th, and 21st days after delivery of Lv-siTLR4 with oral administration of doxycycline, the TLR4 mRNA expression decreased significantly in the Lv-siTLR4 with Dox group compared the other four groups receiving Walker 256 cells injection ( versus Lv-siTLR4 group, Lv-MM group, NS group, and Dox group, two-way ANOVO analysis followed by Tukey’s multiple comparisons, ). No differences were observed on the 1st day after injection.