Nrf2 Deficiency Exaggerates Doxorubicin-Induced Cardiotoxicity and Cardiac Dysfunction
Figure 3
Dox-induced impairment of autophagy in the heart. (a) Male 10–12 wk old WT mice (C57BL/6J) were treated with Dox as in Figure 1 for 4 days and then subjected to measurement of autophagic flux in the heart ( for each group). Upper panel: representative immunoblots as indicated. Lower panel: semiquantified analysis of LC3-II and p62 protein expression. versus Veh. (b) LV lysates as in Figure 2 were subjected to Western blot analysis of polyubiquitinated proteins (Ub-proteins). Upper panel: representative immunoblots as indicated. Lower panel: semiquantified analysis of Ub-proteins level. versus Veh in the same experimental group.
