Oxidative Medicine and Cellular Longevity

Oxidative Medicine and Cellular Longevity / 2019 / Article

Research Article | Open Access

Volume 2019 |Article ID 4651062 | https://doi.org/10.1155/2019/4651062

Nicoletta Guaragnella, Mariarita Stirpe, Domenico Marzulli, Cristina Mazzoni, Sergio Giannattasio, "Acid Stress Triggers Resistance to Acetic Acid-Induced Regulated Cell Death through Hog1 Activation Which Requires RTG2 in Yeast", Oxidative Medicine and Cellular Longevity, vol. 2019, Article ID 4651062, 9 pages, 2019. https://doi.org/10.1155/2019/4651062

Acid Stress Triggers Resistance to Acetic Acid-Induced Regulated Cell Death through Hog1 Activation Which Requires RTG2 in Yeast

Academic Editor: Ralf Braun
Received27 Jul 2018
Accepted13 Dec 2018
Published25 Feb 2019

Abstract

Acid stress causes resistance to acetic acid-induced regulated cell death (AA-RCD) in budding yeast, resulting in catalase activation. In order to explore the molecular determinants of evasion of AA-RCD triggered by acid stress adaptation, we studied the involvement and the possible interplay of the master regulator of transcription high-osmolarity glycerol 1 (HOG1) and RTG2, a positive regulator of the RTG-dependent mitochondrial retrograde signaling. Viability, DNA fragmentation, and ROS accumulation have been analyzed in wild-type and mutant cells lacking HOG1 and/or RTG2. Catalase activity and transcription of CTT1 and CTA1, coding the cytosolic and peroxisomal/mitochondrial catalase, respectively, as well as Hog1 phosphorylation, were also analyzed. Our results show that HOG1 is essential for resistance to AA-RCD and its activation results in the upregulation of CTT1, but not CTA1, transcription during acid stress adaptation. RTG2 is required for Hog1-dependent CTT1 upregulation upon acid stress, despite failure of RTG pathway activation. We give evidence that Rtg2 has a cytoprotective role and can act as a general cell stress sensor independent of Rtg1/3-dependent transcription.

1. Introduction

In multicellular organisms, the controlled suicide of single cells is crucial for development and homeostasis, providing a system that eliminates superfluous cells that might compromise organismal fitness. Similarly, the cellular suicide of a unicellular organism like yeast under certain circumstances provides a system to eliminate cells promoting the survival of the population as a whole. Thus, it is important to understand the mechanisms by which cells activate death or survival pathways in response to environmental changes [1, 2].

Various stress types compromising cell homeostasis elicit the activation of specific adaptive stress response through which extracellular information is converted into rewiring of gene expression aimed at maximizing cell survival [3]. On the other hand, cells of both multicellular and unicellular organisms can succumb through a regulated cell death (RCD) program under extreme conditions [4]. The mechanism by which yeast undergoes RCD in response to acetic acid (AA-RCD) has been investigated in details. Actively dividing yeast cells grown in glucose when shifted to media acidified to pH 3.00 with a strong acid (HCl) and containing 80 mM acetic acid in the undissociated state undergo AA-RCD through a conserved mitochondrial pathway that is characterized by early ROS accumulation, cytochrome c release, and mitochondrial dysfunction, as in mammalian intrinsic apoptosis [5]. We have shown that cell incubation at pH 3.00 (acid stress) for at least twenty minutes before adding acetic acid makes yeast adapted to acetic acid stress and fully resistant to AA-RCD [6].

Acid-stressed yeast cells evade AA-RCD due to a specific increase in catalase activity and decrease in ROS accumulation [6, 7]. Moreover, overexpression of CTT1, encoding cytosolic catalase T, completely inhibits AA-RCD occurrence [8]. Catalase T expression is upregulated under different stressful conditions, including acid stress [9, 10]. The stress-activated protein kinase (SAPK) high-osmolarity glycerol 1 (Hog1) regulates CTT1 transcription through the transcription factors Msn2/Msn4 [10, 11]. Importantly, Hog1 has been linked to acetic acid stress adaptation being responsible for the phosphorylation and subsequent degradation of aquaglyceroporin Fps1, required for cellular accumulation of acetic acid at low pH [12, 13].

Yeast mitochondrial retrograde (RTG) signaling is a mitochondria-to-nucleus communication pathway that affects the transcription of nuclear-encoded mitochondrial genes to compensate for mitochondrial dysfunction, thereby restoring metabolic fitness. Rtg2, the positive regulator of the RTG signaling, acts as a sensor of mitochondrial dysfunction regulating the nuclear localization of the heterodimeric transcription factor Rtg1/3, which controls the transcription of RTG target genes [14]. RTG pathway activation has been shown to contribute to AA-RCD evasion under glucose derepressing conditions, whereby mitochondrial respiration increased [15]. Interestingly, SAPK Hog1 has proved to control Rtg1/3 nuclear accumulation and to regulate its binding to chromatin and transcriptional activity in response to osmostress [16].

The aim of this work was to study the role and the possible interplay of HOG and RTG-dependent signaling in AA-RCD evasion of acid-stressed yeast cells. We demonstrated that both HOG1 and RTG2 contribute to RCD evasion by protecting cells from oxidative stress and mitochondrial dysfunction in response to acetic acid treatment. The expression of CTT1, but not CTA1, encoding peroxisomal catalase A, is upregulated by acid stress in an Rtg2-dependent manner. Finally, our results indicate that Hog1 phosphorylation is delayed in the absence of Rtg2, indicating for the first time a role of RTG signaling in Hog1 activation.

2. Materials and Methods

We followed the methods of Guaragnella et al. [15].

2.1. Yeast Strains, Growth Conditions, and Acetic Acid Treatment

The S. cerevisiae strains used in this study were W303-1B (WT) cells (MATα ade2 leu2 his3 trp1 ura3), ∆rtg2 (rtg2∆::LEU2), and ∆hog1 (hog1∆::NAT#2), which was kindly provided by Prof. Posas, Universitat Pompeu Fabra, Barcelona, Spain. The double mutant ∆rtg2hog1 was constructed by replacing RTG2 with the LEU2 gene (rtg2∆::LEU2 hog1∆::NAT#2).

Cells were grown at 30°C in YPD (1% yeast extract, 2% Bacto peptone, and 2% glucose). Acetic acid treatment was carried out as described in [4]. Briefly, cells were grown at 26°C up to exponential phase () in YPD, resuspended (107 cells/ml) in the same medium adjusted to pH 3.00 with HCl, containing or not (control) 80 mM acetic acid, and incubated for different times at 26°C. Acid-stressed cells were maintained in YPD pH 3.0 medium for 30 minutes before addition of acetic acid. Cell viability was determined by measuring colony-forming units (CFU) after 2 days of growth on YPD plates at 30°C.

2.2. TUNEL Assay, Intracellular ROS Detection, and Respiratory Competence Index

DNA fragmentation was detected by TUNEL assay. Acetic acid-treated and control cells () were harvested at 150 min. Briefly, cells were fixed in a 3.7% formaldehyde solution in PBS, digested with 750 μg/ml zymolyase 20T, and incubated in a permeabilization solution (0.1% Triton X-100, 0.1% sodium citrate) for 2 min on ice and then with a 30 μl TUNEL reaction mixture (In Situ Cell Death Detection Kit, Fluorescein, Roche) for 1 hour at 37°C. After incubation, cells were washed, resuspended in PBS, and observed using a Leica TCS SP5 confocal microscope. To detect intracellular H2O2, 10 μg/ml 2,7-dihydrodichlorofluorescein diacetate (H2DCF-DA, Molecular Probes) dissolved in ethanol was added to cells either 30 min before or during cell treatment with or without acetic acid. acetic acid-treated cells were harvested at 30 min, and oxidation to the fluorophore dichlorofluorescein (DCF) was detected by confocal fluorescence microscopy analysis. Respiratory competence was assessed by measuring cell capacity to grow on nonfermentable and fermentable carbon sources. The same number of cells from acetic acid-treated samples was plated on YPGlycerol (YPG) and YPD, and CFU were counted after 2-3 days. The index of respiratory competence (IRC) was defined as the percentage of cells able to grow in both YPD and YPG [17] and reported as % of acid-stressed WT-adapted cells.

2.3. Catalase Activity Assay

Exponentially grown cells (108), directly exposed to acetic acid or pretreated in acidic medium before the exposure, were sedimented by centrifugation, washed once with 50 mM potassium phosphate buffer pH 7.0, and resuspended in 0.3 ml of the same buffer. Cells were broken through mechanical disruption of cell walls with glass beads (0.5 mm BioSpec Products, Bartlesville, OK, USA) by TissueLyser by vortexing eight times for 30 sec with 30 sec intervals in an ice bath and centrifuged for 10 min (15,000×) to clarify the supernatant. A 20–60 μl supernatant was used for enzyme assay. All reagents used for catalytic activity measurements were from Sigma-Aldrich (St. Louis, MO, USA). The total catalase activity was measured spectrophotometrically by monitoring the disappearance of hydrogen peroxide at 240 nm [18]. Protein concentration was determined using the Bio-Rad protein assay (Bio-Rad Laboratories, Hercules, CA), with bovine serum albumin as a standard.

2.4. Real-Time Polymerase Chain Reaction (PCR)

The mRNA levels of peroxisomal citrate synthase and peroxisomal and cytosolic catalase-encoding genes (CIT2, CTA1, and CTT1) were determined in exponentially growing cells (), after a low pH shift and during acetic acid treatment. 20 ml of cell suspension was centrifuged at 3000×. Cell pellets were either stored at −80°C or immediately used to extract total RNA with Presto Mini RNA Yeast Kit (Geneaid, Taiwan) through mechanical disruption of cell walls with glass beads by TissueLyser (Qiagen). 1 μg RNA () reverse transcription was immediately performed using QuantiTect Reverse Transcription Kit (Qiagen), and cDNA was directly used for real-time PCR analysis or stored at −20°C. Real-time PCR was carried out by QuantiTect SYBR Green PCR Kit (Qiagen) on a QuantStudio 6 Flex instrument from Applied Biosystems using the following primer pairs: for CIT2: (F) 5-CGGTTATGGTCATGCTGTGCT-3 and (R) 5-GGTCCATGGCAAACTTACGCT-3; for CTA1: (F) 5-CAAGCAAGAAATCTCTACCGCG-3 and (R) 5-GCGCTGCTGTATTTGAGGACA-3; for CTT1: (F) 5-GAGAAAGAGTTCCGGAGCGTGT-3 and (R) 5-ATTCTGGTATGGAGCGGCGTA-3; and for ACT1: (F) 5-CTTTGGCTCCATCTTCCATG-3 and (R) 5-CACCAATCCAGACGGAGTACTT-3. The amount of CIT2, CTA1, and CTT1 mRNA normalized with ACT1 mRNA was calculated in arbitrary units (a.u.) using the standard curve method.

2.5. Immunoblot Analysis

Samples of total proteins were extracted according to the TCA method previously described [19], separated by electrophoresis on a denaturing gel, and transferred onto a nitrocellulose filter. After the transfer, the membrane was stained with a Ponceau S solution (Sigma-Aldrich) before immunoblotting analysis. Anti-phospho-p38MAP kinase (Thr180/Tyr182) (#9211, Cell Signaling Technology) and Hog1 (y-215) (sc-9079, Santa Cruz Biotechnology, CA, USA) antibodies (1 : 1000 dilutions) were used to detect phosphorylated Hog1p. Immunodetection was performed using enhanced chemiluminescence (ECL; SuperSignal system, Pierce) detected with an X-ray film (Kodak).

2.6. Statistical Analysis

Statistical analysis was performed by using paired Student’s -test (Excel software).

3. Results

3.1. AA-RCD Evasion due to Acid Stress Depends on HOG1 and RTG2

To investigate the role of HOG1 and RTG pathways in yeast AA-RCD evasion, acid-stressed WT and knockout cells lacking either HOG1 or RTG2 or both genes were compared with respect to cell sensitivity to acetic acid. As a control, WT cells were treated with acetic acid without acid stress adaptation. We found that acid-stressed ∆hog1 cells progressively lose viability which decreased to about 20% at 200 min as for control WT cells that undergo AA-RCD, whereas acid-stressed WT cells remained fully viable, as reported in [6] (Figure 1(a)). Acid-stressed ∆rtg2 cells showed 50% viability after 200 min whereas ∆hog1rtg2 behaved similarly to ∆hog1 cells (Figure 1(a)). The specific death rates of acid-stressed ∆hog1 and ∆hog1rtg2 cells (0.015 min-1) were similar to the ones measured in WT cells undergoing AA-RCD.

In order to assess the nature of cell death, DNA fragmentation was analyzed in acid-stressed cells, both with and without (control) acetic acid treatment. About 90% of ∆hog1 and ∆hog1rtg2 cells treated with acetic acid were positive to TUNEL assay at 150 min (Figure 1(b)). This percentage was similar to the one measured in dying WT cells. Differently, less than 20% of acid-stressed WT cells showed DNA fragmentation. In ∆rtg2 cells, the percentage of DNA fragmentation was more than 50% at 150 min (Figure 1(b)). In the absence of acetic acid treatment, less than 5% of cells were TUNEL positive in all cell types.

Since early ROS accumulation is a typical marker of yeast AA-RCD [5], intracellular hydrogen peroxide levels were measured in WT and mutant cells at 30 min. In ∆hog1 and ∆hog1rtg2 cells, the percentage of DCF-positive cells increased from less than 10% in the control to about 40% following acetic acid treatment (Figure 1(c)). Less than 5% acid-stressed WT cells, which evade AA-RCD, accumulated hydrogen peroxide. Interestingly, in ∆rtg2 cells, ROS accumulation was significantly higher than in acid-stressed WT-adapted cells (20% of positivity to DCF versus 4% measured in WT cells) but still lower than ∆hog1 and ∆hog1rtg2 (Figure 1(c)).

Mitochondrial dysfunction is another hallmark of AA-RCD [20]. Thus, to assess whether the loss of mitochondrial function occurred in the death-sensitive mutants, the index of respiratory competence (IRC) was evaluated as a function of time in acid-stressed ∆hog1, ∆rtg2, and ∆hog1rtg2 cells treated with acetic acid. A reduction of IRC was observed in all cases, with a more drastic decline in functionality for ∆hog1 and ∆hog1rtg2 mutants with respect to ∆rtg2 cells, about 80% versus 60%, respectively, compared to WT-adapted cells after 150 min (Figure 1(d)).

Overall, these results show that HOG1 and partially RTG signaling contribute to AA-RCD evasion due to acid stress.

3.2. RTG2 Is Required for the Full Activation of Catalase T in Acid-Stressed Cells Resistant to AA-RCD

Catalase activity, almost undetectable in exponentially growing WT cells, is specifically triggered by extracellular acid stress reaching its maximum after 30 min and remaining high in cells protected from AA-RCD. Thus, it represents a hallmark of cells evading AA-RCD [6]. Since Hog1 is involved in the osmotic regulation of CTT1 transcription [10], catalase activity was measured in WT and knockout cells either in normal growth conditions without acetic acid (untreated) or at 30 min of acid stress before and after acetic acid addition (Figure 2(a)). As expected, catalase activity was high in WT cells at low pH and remained higher than in untreated cells after the addition of acetic acid. All mutant cells exhibited an overall significant reduction of catalase activity. However, although catalase activity showed a slight but significant increase in acid-stressed Δrtg2 cells with and without acetic acid, it remained virtually constant in ∆hog1 and ∆hog1rtg2 cells in all conditions. These data suggest that RTG2 participates in the HOG1-dependent catalase activity increase caused by acid stress. This is in agreement with the partial and full sensitivity to AA-RCD of rtg2 and hog1 mutants, respectively.

The impairment of the RTG pathway and/or HOG1 deletion partially or fully restored, respectively, AA-RCD in acid-stressed cells with concomitant early accumulation of ROS and a decrease in catalase activity (see Figures 1(c) and 3). Since Hog1 controls the regulation of CTT1 transcription through the transcription factors Msn2/4 [21], we wondered whether RTG2 might have a role in the expression of either CTT1 or CTA1, coding the cytosolic and peroxisomal catalase, respectively. To this aim, CTT1 and CTA1 mRNA levels were measured in WT, ∆rtg2, and/or ∆hog1 cells either in untreated cells or acid-stressed cells before and after acetic acid addition. We found that the CTT1 transcription profile mirrored catalase activity measured in the same cells and under the same conditions (Figure 3(b)). Indeed, RTG2 deletion strongly inhibited Hog1-dependenttranscription in untreated and acid stress conditions. The CTT1 mRNA level is rapidly and transiently increased up to 5-fold after 10 minutes of acid stress (about 70 a.u.) and decreased to about 45 a.u. after 30 min in WT cells. No changes were observed for CTT1 transcription following acetic acid treatment. On the other hand, CTT1 expression was 40 a.u. after 10 min of acid stress and remained constant also during the acetic acid treatment in ∆rtg2 cells (Figure 2(b)). It is of note that in all conditions tested, no CTA1 transcription was observed in any strains analyzed (Figure 2(c)).

These data showed that only catalase T activity is responsible for AA-RCD evasion in acid-stressed cells. They also confirm that CTT1 mRNA upregulation following acid stress strictly depends on SAPK Hog1 kinase and gave the first evidence that Rtg2 is required for Hog1-dependent CTT1 mRNA upregulation in response to acid stress.

3.3. RTG2 Deletion Causes a Delay in Hog1 Phosphorylation

Hog1 activation occurs through its phosphorylation and translocation from the cytoplasm to the nucleus, which is required for transcriptional regulation [22]. Thus, to gain insights into how RTG2 may alter Hog1-dependent transcription, we monitored the Hog1 phosphorylation state in acid-stressed WT or RTG2-lacking cells with or without acetic acid treatment (Figure 4). Under unstressed conditions, the phosphorylated protein was almost undetectable in both WT and mutant cells. Hog1 phosphorylation ensued in acid-stressed WT, but not in ∆rtg2 cells after 5 and 10 minutes. At 30 minutes, while phosphorylation increased in ∆rtg2 cells, a reduction in the amount of phosphorylated protein was observed in WT cells (Figure 3(a)). During acetic acid treatment, Hog1 showed a level of phosphorylation in WT cells virtually similar to that of ∆rtg2 cells at 30, 60, and 90 min (Figure 3(b)).

Thus, RTG2 can modulate the phosphorylation of Hog1 causing a delay in the activation of Hog1 kinase at least in response to acid stress.

3.4. Rtg2 Does Not Activate Rtg1/3-Dependent Retrograde Target Gene Transcription in Acid Stressed Cells

We have previously shown that yeast cells grown in raffinose, which differently from glucose, promotes respiration, evade AA-RCD due to RTG pathway activation [15, 23]. In order to check if RTG pathway activation can contribute to AA-RCD evasion of acid-stressed cells, the mRNA level of CIT2, encoding the peroxisomal isoform of citrate synthase, whose upregulation is a hallmark of Rtg1/3-dependent transcription activation [14], was measured in WT and ∆hog1 mutant cells either in normal growth conditions without acetic acid (untreated) or during acid stress before and after acetic acid addition (Figure 4). The level of expression was about 5 a.u. in the absence of stress. Interestingly, about a 2-fold reduction of the CIT2 mRNA level of expression was measured in ∆hog1 cells under normal growth conditions. The CIT2 mRNA level showed about an 80% decrease in acid-stressed WT cells with respect to normal growth conditions and exhibited a virtually constant value either in the absence or in the presence of acetic acid. This shows that the RTG pathway was not activated by acid stress. On the other hand, the CIT2 mRNA level remained unchanged as compared with normal growth conditions in acid-stressed ∆hog1 cells with or without acetic acid treatment (Figure 5). As expected, CIT2 expression was completely abolished in the ∆rtg2 mutant.

These data demonstrate that the RTG signaling does not contribute to AA-RCD evasion in cells adapted by acid stress. In addition, Hog1 is required for the full transcriptional activation of CIT2 in normal growth conditions as well as for the CIT2 transcription regulation in response to stress.

4. Discussion

Yeast cells can evade AA-RCD either upon acid stress adaptation, due to the cytoprotective role of the catalase upregulation, or under conditions of concomitant derepression of mitochondrial respiration and RTG pathway activation, like in raffinose-grown cells [6, 8, 15]. In this work, we showed a mutual interplay between RTG2, a positive regulator of the mitochondrial RTG pathway, and SAPK HOG1, the master regulator of catalase gene transcription, in coordinating stress response and modulating resistance to AA-RCD due to acid stress adaptation.

Our data demonstrate that both HOG1 and RTG2 contribute to AA-RCD evasion, with an essential requirement of HOG1 (Figures 1(a) and 2(b)). These two genes seem to exert their protective role against acetic acid toxicity by increasing oxidative stress resistance and preserving mitochondrial function (Figures 1(c) and 1(d)). The requirement of RTG2 for CTT1 activation, as shown in Figure 3(a), supports the involvement of the RTG pathway in oxidative stress resistance as already shown in [13, 24, 25]. Complete preservation of mitochondrial function in acid stress cells evading AA-RCD was abolished by HOG1 deletion, confirming the link of the MAPK pathway with mitochondrial dysfunction already observed upon osmotic stress and in pathogenic fungi [24, 26, 27].

Here, we showed that specific activation of cytosolic catalase T, but not peroxisomal/mitochondrial catalase A, is responsible for acid stress cell evasion of AA-RCD (Figure 2). This is in agreement with previous findings that CTT1 overexpression can fully prevent AA-RCD [8]. Thus, detoxification of hydrogen peroxide together with catalase activation appears to be a key condition for acetic acid tolerance and in general stress cotolerance as reported in [28].

How RTG2 interacts with HOG1 to regulate CTT1 transcription remains to be established. Our analysis of Hog1 phosphorylation en route to acid stress clearly shows that RTG2 deletion significantly delays Hog1 activation through phosphorylation, suggesting possible interactions with Hog1-specific kinases (Figure 3). On the other hand, HOG1 has been involved in the regulation of Rtg1/3 complex activity in response to osmostress [16]. According to these results, our data showed that HOG1 could exert a modulatory effect on Rtg1/3-dependent transcription depending on the metabolic state of the cell: Hog1 is required for the full transcriptional activation of the CIT2 gene in normally growing cells, but it negatively regulates RTG pathway activation in stressed cells (Figure 4). This is in agreement with the increase of CIT2 expression occurring in a Hog1-dependent manner upon osmostress [16]. Overall, our data clearly demonstrate the interplay between Rtg2 and Hog1 (Figure 5), whose mechanism deserves further investigations. However, since Rtg2 can bind to chromatin at the promoters of regulated genes as a component of a SAGA-like complex, named SLIK [29], and an interaction between Hog1 SAPK and SAGA complex has been previously reported upon severe osmostress [22, 3032], we can hypothesize that Rtg2 and Hog1 interact at the level of epigenetic regulation of gene expression. In any case, our results clearly show a strict cooperation of HOG and RTG pathways to induce a hormetic response of yeast cells to different kinds of stresses.

It is worth noting that HOG pathway activation can rescue cells from various cellular dysfunctions caused by the impaired biosynthesis of complex sphingolipids through a yet unknown molecular mechanism [33]. It has been suggested that the HOG pathway could sense impaired formation of lipid microdomains, which are formed by complex sphingolipids and sterol molecules [34]. Since others and we have shown that ceramide production contributes to mitochondrial dysfunction and AA-RCD [35, 36], our results support the hypothesis (it is tempting to speculate) that acid stress could activate Hog1 through a mechanism alike that ensues a sphingolipid metabolism defect.

Differently from raffinose-grown cells which evade AA-RCD mostly due to RTG pathway activation [15], acid stress does not activate Rtg1/3-dependent mitochondrial retrograde target gene transcription for cell adaptation (Figure 4). Thus, different branches of the RTG-dependent signaling can be activated depending on the type of external stimuli, suggesting that RTG2 may have a complex role in the integration of different stress signaling pathways through yet unknown regulatory elements [37].

5. Conclusions

By further exploring the molecular basis of yeast tolerance to acetic acid, this work highlights the importance of detailed understanding of the interplay between signaling pathways in stress response. In particular, it provides novel insights into the strict relationship between cell stress adaptation and oxidative stress resistance ensuing from the cooperation of RTG- and HOG1-mediated stress signaling pathways. When activated, these pathways converge to produce a hormetic response in cells undergoing environmental stresses. The discovery of the new role for RTG2 in modulating Hog1 activation will deserve deeper investigations to understand conditions and modes of this interaction upon stress. These results could be relevant for certain industrial microbial applications in which acetic acid accumulation is detrimental and the improvement of strain resistance to acetic acid is highly desirable.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

Acknowledgments

The authors thank Dr. Laura Marra for her administrative and technical assistance and are grateful to Dr. Ersilia Marra for her support and critical advices in starting this project. This work was supported by Progetto POR Puglia FESR-FSE 2014-2020 Azione 1.6. Avviso “Innonetwork” (grant number 1JLZKD0).

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Copyright © 2019 Nicoletta Guaragnella et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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