New 2-Acetyl-3-aminophenyl-1,4-naphthoquinones: Synthesis and <i>In Vitro</i> Antiproliferative Activities on Breast and Prostate Human Cancer Cells

Ríos, David; Valderrama, Jaime A.; Cautin, Miriam; Tapia, Milko; Salas, Felipe; Guerrero-Castilla, Angélica; Muccioli, Giulio G.; Buc Calderón, Pedro; Benites, Julio

doi:https://doi.org/10.1155/2020/8939716

Oxidative Medicine and Cellular Longevity

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments Supplementary Materials References Copyright Related Articles

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Pharmaceutical and Pharmacological Aspects of Modulation of Oxidative Stress 2020

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Volume 2020 | Article ID 8939716 | https://doi.org/10.1155/2020/8939716

New 2-Acetyl-3-aminophenyl-1,4-naphthoquinones: Synthesis and In Vitro Antiproliferative Activities on Breast and Prostate Human Cancer Cells

David Ríos,¹Jaime A. Valderrama,¹Miriam Cautin,¹Milko Tapia,¹Felipe Salas,¹Angélica Guerrero-Castilla,¹Giulio G. Muccioli,²Pedro Buc Calderón,^1,3and Julio Benites¹

Academic Editor: Luciano Saso

Received31 May 2020

Revised27 Jul 2020

Accepted28 Aug 2020

Published26 Sept 2020

Abstract

The reaction of 2-acyl-1,4-naphthoquinones with N,N-dimethylaniline and 2,5-dimethoxyaniline, promoted by catalytic amounts of CeCl₃·7H₂O under “open-flask” conditions, produced a variety of 2-acyl-3-aminophenyl-1,4-naphthoquinones structurally related to the cytotoxic 2-acetyl-3-phenyl-1,4-naphthoquinone, an inhibitor of the heat shock chaperone protein Hsp90. The members of the 2-acyl-3-aminophenyl-1,4-naphthoquinone series were isolated in good yields (63-98%). The cyclic voltammograms of the 2-acyl-3-aminophenyl-1,4-naphthoquinone exhibit two one-electron reduction waves to the corresponding radical-anion and dianion and two quasireversible oxidation peaks. The first and second half-wave potential values () of the members of the series are sensitive to the push-pull electronic effects of the substituents in the naphthoquinone scaffold. Furthermore, the in vitro antiproliferative properties of these new quinones were evaluated on two human cancer cells DU-145 (prostate) and MCF-7 (mammary) and a nontumorigenic HEK-293 (kidney) cell line, using the MTT colorimetric method. Two members, within the series, exhibited interesting cytotoxic activities on human prostate and mammary cancer cells.

1. Introduction

The electroactive naphthoquinone core is a common structural constituent of a variety of biochemical systems involved in the human defense system [1]. Several biological active quinones as ubiquinone and vitamin K are known to be electron transporters and are essential for many enzymatic processes. They can act as anti- or prooxidants depending on the nature of the media: this chemical versatility gives them an important role in different biochemical processes that are essential to living organisms [2]. The oxidation state of naphthoquinones allows them to act by different mechanisms, such as free radical scavengers, metal ion chelators, and also enzyme inhibitors for free radical production [3, 4]. This imbalance between the formation and removal of ROS (reactive oxygen species) causes damage to the cells at nucleic acids, proteins, and membrane lipids associated with ageing, carcinogenesis, cardiovascular, and coronary diseases [5]. In this context, the naturally occurring 2-hydroxy-1,4-naphthoquinone (lawsone) exhibits a number of interesting biological activities, such as antioxidant [6], antibacterial, antifungal [7], anti-inflammatory, antipyretic, analgesic [8], and anticancer cytotoxic [9]. Therefore, the quinones could play an important role in inhibiting or delaying oxidative stress that arises from an imbalance between free radical production and antioxidant and repair defenses [10].

Acylated 1,4-quinones such as 2-acyl-1,4-benzo- and 2-acyl-1,4-naphthoquinones are a valuable building block of several natural [1, 11–13] and synthetic compounds endowed with a wide range of biological activities [14–19]. Acylquinones are highly reactive towards nucleophiles due to the confluence of electrophilic centers at the quinone nucleus and the 2-carbonyl substituents. The relatively close location of these electrophilic centers in the acylquinone scaffold enables reactions with diverse mono- and binucleophiles such as arylamines [17], azaenamines [18], enaminones [19], and 2-aminobenzothiazoles [20] to give a diversity of substances shown in Figure 1 such as 2,3-disubstituted 1,4-naphthoquinones (a, b) and heterocyclic fused 1,4-naphthoquinones (c–e).

(a)

(b)

(c)

(d)

(e)

High-throughput screening of a library of structurally diverse compounds has identified, among 120 active members, the 1,4-naphthoquinone derivatives, named HTS1 and HTS3, as a new class of inhibitors of the heat shock chaperone protein Hsp90 (Figure 2). The protein Hsp90, which ensures protein homeostasis in the presence and in the absence of cellular stress, is unique because most of its client proteins are conformationally labile signal transducers that play a crucial role in cell growth control, survival, and development processes [11]. Hsp90 represents 1–2% of the total protein cellular content, but its expression is enhanced by 2–10-fold in cancer cells [12], thus making it an attractive goal for the development of Hsp90 inhibitors [1, 13–15] and, consequently, a relevant target in cancer therapy [21]. Both HTS1 and HTS3 have been shown antiproliferative activity against human cancer mammary cells. For instance, HTS1 showed 4-fold greater antiproliferative activity against MCF-7 cells (an estrogen-dependent breast cancer cell line) compared to SKBr3 cells (an Her2-overexpressing breast cancer cell line), indicating that this scaffold may provide a useful probe to study estrogen-dependent cancers. On the other hand, HTS3 shows equal activity against both mammary cancer cells. In addition, the members I-III induced the degradation of oncogenic Hsp90 client proteins, a hallmark of Hsp90 inhibition [22].

Recently, we have reported the synthesis of a variety of 2-acyl-3-phenylamino-1,4-naphthoquinones (i.e., compound IV in Figure 2) prepared by oxidative amination of 2-acylnaphthoquinones with phenylamines. The members of this naphthoquinone series were designed as potential inhibitors of Hsp90 chaperoning function due to their close structural similarity to that of HTS1. The in vitro screening of the series demonstrated cytotoxic activity on cancer cells [23], and the congener IV (Figure 2) exhibited action as an inhibitor of Hsp90 chaperoning function [19].

Older studies reported by Pardo et al. [24] demonstrated that the reaction of 2-acetylnaphthohydroquinone with phenylamines, under oxidant conditions, takes place in a complex manner. Indeed, it yields four types of products depending upon the structure of the arylamine and the medium solvent: amination (C-N bond formation), arylation (C-C bond formation), arylation-cyclisation (C-C and C=N bond formations), and arylation-amination (C-C and N-C bond formations) [24, 25]. These reactions occur between the “nascent” 2-acetyl-1,4-naphthoquinone, in situ generated by oxidation of acetylnaphthohydroquinone with sodium periodate, and the arylamines. Recent studies performed in our laboratory show that the reaction of diverse 2-acylnaphthoquinones with 3,4,5-trimethoxyaniline in methanol yields amination products (C-N bond formation) together with two types of arylation-cyclisation products (C-C and two alternative N=C or N-C bond formations). We have not detected arylation products in these assays probably due to the fact that they undergo fast cyclisation reactions to produce their respective arylation-cyclisation products [26].

Pardo et al. [24] reported the synthesis of arylation products, named as 2-acetyl-3-aminophenyl-1,4-naphthoquinones (Scheme 1), which are closely related to HTS1 (2-acetyl-3-phenyl-1,4-naphthoquinone) shown in Figure 2. Inspired by such structural similarities, we wanted to explore the acylquinone scope of this arylation reaction to the synthesis of 2-acyl-3-aminophenylnaphthoquinones from a representative number of acylnaphthoquinones, N,N-dimethylaniline and 2,5-dimethoxyaniline.

In this paper, we report flexible access to 2-acyl-3-aminophenyl-1,4-naphthoquinones prepared from 2-acylnaphthohydroquinones, N,N-dimethylaniline and 2,5-dimethoxyaniline. The half-wave potentials of such new HTS1 analogues were calculated, and they were evaluated for their in vitro antiproliferative activities on two human-derived cancer cell lines DU-145 (prostate) and MCF-7 (breast) and nontumorigenic HEK-293 (kidney) cells using as endpoint the MTT colorimetric method.

2. Materials and Methods

2.1. General Information

All the solvents and reagents were purchased from different companies, such as Aldrich (St. Louis, MO, USA) and Merck (Darmstadt, Germany), and were used as supplied. Melting points (mp) were determined on a Stuart Scientific SMP3 (Staffordshire, UK) apparatus and are uncorrected. The record of IR, ¹H- and ¹³C-NMR spectra, and chromatography procedures were done according to methods reported by Benites et al. [27]. Proton nuclear magnetic resonance (¹H NMR) spectra were measured at 300 MHz in a Bruker Ultrashield-300 spectrometer. Data for the ¹H NMR spectra are reported as follows: s = singlet, br s = broad singlet, d = doublet, and t = triplet, and the coupling constants () are in Hz. Carbon-13 nuclear magnetic resonance (¹³C NMR) spectra were measured at 75 MHz in a Bruker Ultrashield-300 spectrometer. Bidimensional NMR techniques and distortion-less enhancement by polarization transfer (DEPT) were used for the signal assignment. Chemical shifts are expressed in ppm downfield relative to tetramethylsilane, and the coupling constants () are reported in Hertz. The high-resolution mass spectrometry (HRMS) data for all final compounds were obtained using a LTQ-Orbitrap mass spectrometer (Thermo-Fisher Scientific, Waltham, MA, USA) with the analysis performed using an atmospheric-pressure chemical ionization (APCI) source, operated in a positive mode. The acylnaphthohydroquinones (2-8) were prepared according to a previously reported procedure [28].

2.2. Chemistry

2.2.1. Preparation of 2-Acyl-3-aminophenylnaphthoquinones (16-28): General Procedure

Suspensions of the acylnaphthohydroquinones 2–8 (1.0 mmol), Ag₂O (2.0 equiv.), and MgSO₄ anhydrous (300 mg) in dichloromethane (30 mL) were left with stirring for 30 min at room temperature (rt). The mixtures were filtered, the solids were washed with dichloromethane ( mL), and the filtrates containing the respective 2-acyl-1,4-naphthoquinones were evaporated under reduced pressure. The residues were dissolved in methanol (15 mL), the phenylamines (2 equiv.) and CeCl₃·7H₂O (5% mmol) were added to the solutions, and the mixtures were left with stirring at rt according to the times collected in Table 1. The solvents were removed under reduced pressure, and the residues were column-chromatographed over silica gel (petroleum ether/EtOAc) to yield the corresponding pure 2-acyl-3-aminophenylnaphthoquinones 16-28.

2.3. Biological Assays

2.3.1. Cell Lines and Cell Cultures

Human cancer cell lines MCF-7 (mammary) and DU-145 (prostate) and nontumor HEK-293 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cultures were maintained at a density of cells/mL, and the medium was changed at 48 to 72 h intervals. They were cultured in high-glucose Dulbecco’s modified Eagle medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal calf serum, penicillin (100 U/mL), and streptomycin (100 μg/mL). All cultures were kept at 37°C in 95% air/5% CO₂ at 100% humidity. Phosphate-buffered saline (PBS) was purchased from Gibco. Cells were incubated at the indicated times at 37°C, with or without quinones at various concentrations.

2.3.2. Cytotoxic Assays

The cytotoxicity of the quinones was assessed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction assays [29], according to Valderrama et al. [30]. Briefly, adherent cells were detached by using trypsin/EDTA solution. The culture medium was removed, and cells were washed with a free Ca-Mg salt solution to remove all traces of serum. After removing salt solution, trypsin/EDTA solution was added to completely cover the monolayer of cells for 2-3 min at 37°C. When the trypsinization process was completed, trypsin/EDTA was removed by aspiration and cells were resuspended, diluted in fresh medium, and seeded for 24 h into 96-well plates at a density of 10,000 cells/well. Then, they were further incubated for 48 h, with or without the quinone derivatives. Doxorubicin was used as the standard chemotherapeutic agent (positive control) in a dose range of 0.01 to 10 μM. Cells were then washed twice with warm PBS, and they were further incubated with MTT (0.5 mg/mL) for 2 hours at 37°C. Blue formazan crystals were solubilized by adding 100 μL DMSO/well, and the optical density of the colored solutions was subsequently read at 550 nm. Results are expressed as a percentage of MTT reduction, compared to untreated control conditions. The IC₅₀ values were calculated using the GraphPad Prism software (San Diego, CA, USA).

3. Results and Discussion

3.1. Chemistry

The 2-acylnaphthoquinones 9-15 selected for the study were prepared from the 2-acylnaphthohydroquinones 2-8 according to Scheme 2. The hydroquinone precursors 2-8 were synthesized by solar photo-Friedel-Crafts acylation of 1,4-naphthoquinone 1 with the following aldehydes: acetaldehyde, butyraldehyde, hexanal, 4-methoxybenzaldehyde, 2,5-dimethoxybenzaldehyde, 2-furancarbaldehyde, and 2-thiophencarbaldehyde, according to our previously reported procedure [28]. Access to the 2-acyl-1,4-naphthoquinones 9-15 was accomplished by oxidation of 2-acylnaphthohydroquinones 2-8 with silver (I) oxide in dichloromethane in the presence of dry magnesium sulphate [19]. The resulting acylnaphthoquinones isolated from the mixture reactions were dissolved in methanol and immediately reacted with the N,N-dimethylaniline and 2,5-dimethoxyaniline (Supplementary Materials (available here)).

Among the members of the acylnaphthoquinone series, compound 14 was selected to get preliminary insights into its reactivity to undergo arylation reaction with N,N-dimethylaniline. In a preliminary assay, acylnaphthoquinone 14, prepared from acylnaphthohydroquinone 7, was reacted with N,N-dimethylaniline in methanol at rt. The reaction carried out under open-flask conditions to favor aerobic oxidation reactions takes place slowly to give, after 72 hours, the arylation product 20 in 73% yield, referred to precursor 7. Furthermore, it was observed that the reaction of 14 with N,N-dimethylaniline in refluxing ethanol takes place relatively faster (33 hours) than in methanol, but compound 20 is produced in moderate yield (51%).

The validity of the arylation mechanism of acylquinone 9 with arylamines proposed by Pardo et al. [24] was assumed to proceed through a sequence of a Michael addition reaction followed by aerobic oxidation of the adduct intermediate. Therefore, we envisaged to improve the arylation reaction of 14 (Table 1) with N,N-dimethylaniline by using a green Lewis acid catalyst such as CeCl₃·7H₂O [31] or IBr₃ [32]. Successful results on the use of the Ce(III) catalyst in the oxidative amination reaction of quinones with arylamines have been reported in literature [32–38]. These acid catalysts probably increase the electrophilic character of the enone system of the quinones, via coordination with the oxygen atom of the carbonyl group, thus promoting the Michael-type addition [35, 39, 40].

We examined the reactions of 14 with N,N-dimethylaniline in the presence of catalytic amounts of CeCl₃·7H₂O and IBr₃ (5%) in methanol, under open-flask conditions at room temperature. Both reactions occurred faster (28 h) than in the absence of these Lewis acids (72 h), and the arylation product 20 (Table 1) was isolated in 98 and 78% yield, respectively. The successful results obtained in the assay employing Ce(III) led us to evaluate the scope of the arylation reaction of the remaining members of the series with the selected arylamines, under the above optimized conditions. The results of the assays are summarized in Table 1.

The structures of compounds 16–28 were established by ¹H- and ¹³C-nuclear magnetic resonance (NMR), bidimensional nuclear magnetic resonance (2D-NMR), and high-resolution mass spectrometry (HRMS).

The data in Table 1 show that the arylation reaction of 2-acylnaphthoquinones 9-15 with the arylamines, catalyzed with Ce(III), yields the corresponding 2-acetyl-3-aminoaryl-1,4-naphthoquinones with good to excellent yields ranging from 63 to 98%, except in the case of product 17 (33%). Comparison of the reaction time formation of compounds 16-28 reveals two facts: the first one was linked to the lower reactivity of N,N-dimethylaniline compared to 2,5-dimethoxyaniline and secondly the strong influence of the stereoelectronic nature of the acyl substituents on the nucleophilic attack of the arylamines. It has to be mentioned that no attempts were made in order to decrease the reaction time formation of the arylation products. Based on our recent results [41] and that reported by Liu and Ji [42], on the arylamination of quinones, we will attempt, in future researches, the use of ultrasound to accelerate the Ce(III)-promoted arylation reaction of acylnaphthoquinones with arenes in order to expand the 2-acetyl-3-aminoaryl-1,4-naphthoquinone series for further biological studies.

The acid-induced formation of the arylation compounds 16-28 from acylquinones 9-15 and their reaction with N,N-dimethylaniline are proposed to occur according to the mechanism of reaction depicted in Scheme 3. This approach is based on the reaction mechanism for the Ce(III)-promoted phenylamination reaction of 1,4-naphthoquinone with 2-fluoro- and 2-methoxyanilines reported by Leyva et al. [35]. Initially, a selective conjugate Michael-type addition of the arylamine across the enone system C₃=C₂-C₁=O seems plausible. This reaction, which involves the high electrophilic C-3 of the acylquinones, provides the respective C-C Michael adduct intermediates. Further enolization of these species, followed by aerobic oxidation, gives compounds 16-21. It should be noted that the electrophilicity of the C-3 in these acylquinones is mainly due to the electron-withdrawing effects of the acyl substituents attached to the 2-position. The coordination of Ce(III) to the oxygen atom of the enone system should contribute to increasing the electrophilicity of the C-3.

An interesting feature of the synthetized arylation products is their strong purple color of the chromophores that, according to Pardo et al. [24], is due to the strong donor-acceptor interactions between the quinoid and the electron-rich nitrogen substituents. Figure 3 shows the hybrid structures of compounds 16 and 23 where such interactions are clearly observable. Inspection of minimal energy conformation of compounds 16 and 23 performed by MM2 calculation (ChemBio3D 11.0, PerkinElmer, MA, USA) shows a noncoplanar orientation between the acyl-carbonyl groups and the naphthoquinone framework. This data agrees with the infrared absorption of the acyl-carbonyl groups of these compounds 16–28 that appeared within the range: ν_máx 1706-1735 cm^-1.

3.2. Voltammetric Measurements and Antioxidant Activities

In order to assess the redox properties of the members of the 2-acyl-3-aminoaryl-1,4-naphthoquinone series 16–28, their half-wave potentials and were measured by cyclic voltammetry. The measurement was conducted in acetonitrile at room temperature, using a platinum electrode and 0.1 M tetraethylamoniumtetrafluoroborate as the supporting electrolyte [19]. The voltammograms were recorded in the potential range from 0.0 to -2.0 V vs. nonaqueous Ag/Ag⁺. Figure 4 shows the typical electrochemical behavior of the arylation compound 23 that proceeded in two one-electron diffusion stages.

The cathodic peaks related to the reduction of quinone nucleus, and the anodic one due to its reoxidation, were observed for compound 23 as well-defined quasireversible waves. The values for the first one-electron correspond to the semiquinone radical anion formation and the second one-electron transfer to the dianion formation [17]. The magnitude of these values falls within the ranges −875 to −620 mV/−1295 to −1125 mV for the members of the 2-acyl-3-(4-N,N-dimethylaminophenyl)naphthoquinones 16-21 and -890 to -685 mV/-1410 to -1180 mV for the members of the 2-acyl-3-(4-amino-2,5-dimethoxyphenyl)naphthoquinones 22-28 (Table 2).

The notable differences of the and values could be attributed to the stereoelectronic effects of the acyl substituents in the naphthoquinone scaffold (R = methyl, 1-propyl, 1-pentyl, 4-methoxyphenyl, 2,5-dimethoxyphenyl, 2-furyl, and 2-thienyl) in the 4-N,N-dimethylamino- and 4-amino-2,5-dimethoxyphenyl-1,4-naphthoquinone. Interestingly, the series 22-28 have lower values compared to 16-21, with the only exception of 20.

3.3. Antitumor Activity

The 2-acyl-3-aminophenylnaphthoquinones 16-28 were evaluated for in vitro cytotoxic activities against nontumorigenic human embryonic kidney cells (HEK-293 cells) and two human cancer cell lines DU-145 (prostate) and MCF-7 (mammary) in 72 h drug exposure assays. The cytotoxic activities of the new compounds were measured using conventional microculture tetrazolium reduction assays [29]. Such activities are expressed in terms of IC₅₀. Doxorubicin, a well-known anticancer agent currently used in clinical practice, was taken as a positive control. The cytotoxic activity data are summarized in Table 3.

The data indicated that, in general, the DU-145 cells are more sensitive than MCF-7 cells to the compounds. In addition, the 2-acyl-3-(4-amino-2,5-dimethoxyphenyl)naphthoquinone members 22-28 exhibited higher activity than their corresponding analogues 16-21 but lower than those displayed by doxorubicin. Among the 2-acyl-3-(4-dimethylaminophenyl)naphthoquinone members, those containing the furan-2-carbonyl and thiophene-2-carbonyl groups, as compounds 20 and 21, are the most active on the DU-145 cancer cells. Inspection of the biological activity of 2-acyl-3-(4-amino-2,5-dimethoxyphenyl)naphthoquinone members reveals that those containing acetyl and furan-2-carbonyl groups, as 22 and 27, exhibited the higher activity on DU-145 and MCF-7 cancer cell lines. However, by taking the mean IC₅₀ value and calculating a selectivity index (IC₅₀ HEK-293 value/IC₅₀ cancer cell value), compound 22 displays a better index (1.71) than 27 (0.65), suggesting that this latter quinone did not discriminate between cancer and healthy cells. By increasing the number of the aliphatic chain within the acyl substituents, a decrease in the antiproliferative activity is observed: compounds 22 and 23 are more active than 24. The screening also shows that the members having 4-methoxy- and 2,5-dimethoxybenzoyl substituents, as compounds 18, 19, and 25 show weak or almost nonexistent cytotoxic activity. Finally, an additional element may be proposed to explain why compound 22 displays the best activity among the series: namely, its strong electronegative redox potential. In this regard, it is tempting to speculate that compound 22 has higher redox cycling ability, and therefore, it may generate the largest amount of ROS. Indeed, quinone redox cycling has been evoked as the main molecular mechanism explaining numerous deleterious effects by such molecules [43–46].

4. Conclusions

We have developed a simple and flexible route for the synthesis of novel 2-acyl-3-(aminophenylamino)-1,4-naphthoquinones whose structure is related to the HTS1, an inhibitor of the heat shock protein Hsp90. The members of the series were prepared in moderate to good yields (63–98%) by reaction of acylnaphthoquinones with phenylamines, catalyzed by CeCl₃·7H₂O. The new quinones were characterized by their spectral data and half-wave potentials. The first and second half-wave potential values () of the members of the series are sensitive to the acceptor and donor electronic effects of the substituents located in the quinone double bond. The in vitro cytotoxicities of the compounds on cancer cells were determined by using the MTT colorimetric method. The results of the biological evaluation of the 2-acyl-3-(aminophenyl)-1,4-naphthoquinone series show interesting in vitro cytotoxic activity on DU-145 and MCF-7 cancer cell lines for the member 22 making it a suitable new chemical entity to be further developed.

Data Availability

All data to be shared are included in the main text as well as in Supplementary Materials.

Conflicts of Interest

The authors declare no conflict of interest.

Acknowledgments

We thank Pilar Díaz from the Universidad Arturo Prat, Chile, for her excellent technical support and to Fondo Nacional de Ciencia y Tecnología (FONDECYT), grant numbers 11140544 and 1190577, and Proyecto Interno Universidad Arturo Prat VRIIP0030-17 for the financial support given to this study.

Supplementary Materials

Description of the spectral data of nuclear magnetic resonance (¹H NMR and ¹³C NMR) and high-resolution mass spectrometry (HRMS) of 2-acyl-3-aminophenylnaphthoquinones. (Supplementary Materials)

References

R. H. Thompson, Naturally Occurring Quinones IV: Recent Advances, Blackie Academic & Professional, London, UK, 4th edition, 1997.
G. Lenaz, R. Fato, A. Baracca, and M. L. Genova, “Mitochondrial quinone reductases: complex I,” in Methods Enzymology, vol. 382, pp. 3–20, Elsevier, 2004.
View at: Publisher Site | Google Scholar
J. M. C. Gutteridge and B. Halliwell, “Free radicals and antioxidants in the year 2000. A historical look to the future,” The Annals of the New York Academy of Sciences, vol. 899, no. 1, pp. 136–147, 2000.
View at: Google Scholar
B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, Oxford University Press, Oxford, 4th edition, 2006.
S. Y. Wang and H. Jiao, “Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen,” Journal of Agricultural and Food Chemistry, vol. 48, no. 11, pp. 5677–5684, 2000.
View at: Publisher Site | Google Scholar
M. A. Omar, “Effect of 2-hydroxy-1, 4-naphthoquinone, a natural dye of Henna, on aldehyde oxidase activity in guinea pig liver,” Journal of Medical Sciences, vol. 5, no. 3, pp. 163–168, 2005.
View at: Google Scholar
V. Ambrogi, D. Artini, I. Carneri et al., “Studies on the antibacterial and antifungal properties of 1, 4-naphthoquinones,” British Journal of Pharmacology, vol. 40, no. 4, pp. 871–880, 1970.
View at: Publisher Site | Google Scholar
B. H. Ali, A. K. Bashir, and M. O. Tanira, “Anti-inflammatory, antipyretic, and analgesic effects of Lawsonia inermis L.(Henna) in rats,” Pharmacology, vol. 51, no. 6, pp. 356–363, 1995.
View at: Publisher Site | Google Scholar
H. Kamei, T. Koide, T. Kojima, Y. Hashimoto, and M. Hasegawa, “Inhibition of cell growth in culture by quinones,” Cancer Biotherapy and Radiopharmaceuticals, vol. 13, no. 3, pp. 185–188, 1998.
View at: Publisher Site | Google Scholar
B. J. Day, “Antioxidant therapeutics: Pandora’s box,” Free Radical Biology and Medicine, vol. 66, pp. 58–64, 2014.
View at: Publisher Site | Google Scholar
K. Maruyama and Y. Naruta, “Syntheses of α- and β-lapachones and their homologues by way of photochemical side chain introduction to quinone,” Chemistry Letters, vol. 6, no. 8, pp. 847–850, 1977.
View at: Publisher Site | Google Scholar
H. Uno, “Allylation of 2-alkanoyl 1,4-quinones with allylsilanes and allylstannanes. Efficient synthesis of pyranonaphthoquinone antibiotics,” The Journal of Organic Chemistry, vol. 51, no. 3, pp. 350–358, 1986.
View at: Publisher Site | Google Scholar
M. A. Brimble and S. M. Lynds, “A short synthesis of deoxyfrenolicin,” Journal of the Chemical Society, Perkin Transactions, vol. 1, no. 5, pp. 493–496, 1994.
View at: Google Scholar
G. A. Kraus and H. Maeda, “A direct preparation of 1,4-benzodiazepines. The synthesis of medazepam and related compounds via a common intermediate,” Tetrahedron Letters, vol. 35, no. 49, pp. 9189-9190, 1994.
View at: Publisher Site | Google Scholar
P. A. Waske, J. Mattay, and M. Oelgemöller, “Photoacylations of 2-substituted 1,4-naphthoquinones: a concise access to biologically active quinonoid compounds,” Tetrahedron Letters, vol. 47, no. 8, pp. 1329–1332, 2006.
View at: Publisher Site | Google Scholar
J. A. Valderrama, J. Benites, M. Cortés, D. Pessoa-Mahana, E. Prina, and A. Fournet, “Studies on quinones. Part 35: access to antiprotozoal active euryfurylquinones and hydroquinones,” Tetrahedron, vol. 58, no. 5, pp. 881–886, 2002.
View at: Publisher Site | Google Scholar
D. R. Vásquez, J. Verrax, J. A. Valderrama, and P. B. Calderon, “Aminopyrimidoisoquinolinequinone (APIQ) redox cycling is potentiated by ascorbate and induces oxidative stress leading to necrotic-like cancer cell death,” Investigational New Drugs, vol. 30, no. 3, pp. 1003–1011, 2012.
View at: Publisher Site | Google Scholar
V. Delgado, A. Ibacache, C. Theoduloz, and J. A. Valderrama, “Synthesis and in vitro cytotoxic evaluation of aminoquinones structurally related to marine isoquinolinequinones,” Molecules, vol. 17, no. 6, pp. 7042–7056, 2012.
View at: Publisher Site | Google Scholar
D. Rios, J. Benites, J. A. Valderrama et al., “Biological evaluation of 3-acyl-2-arylamino-1,4-naphthoquinones as inhibitors of Hsp90 chaperoning function,” Current Topics in Medicinal Chemistry, vol. 12, no. 19, pp. 2094–2102, 2012.
View at: Publisher Site | Google Scholar
J. A. Valderrama, D. Ríos, G. G. Muccioli, P. Buc Calderon, I. Brito, and J. Benites, “Hetero-annulation reaction between 2-acylnaphthoquinones and 2-aminobenzothiazoles. A new synthetic route to antiproliferative benzo[g]benzothiazolo[2,3-b]quinazoline-7,12-quinones,” Tetrahedron Letters, vol. 56, no. 36, pp. 5103–5105, 2015.
View at: Publisher Site | Google Scholar
J. Benites, J. A. Valderrama, M. Ramos, G. G. Muccioli, and P. Buc Calderon, “Targeting Akt as strategy to kill cancer cells using 3-substituted 5-anilinobenzo[c]isoxazolequinones: a preliminary study,” Biomedicine & Pharmacotherapy, vol. 97, pp. 778–783, 2018.
View at: Publisher Site | Google Scholar
M. K. Hadden, S. A. Hill, J. Davenport, R. L. Matts, and B. S. J. Blagg, “Synthesis and evaluation of Hsp90 inhibitors that contain the 1,4-naphthoquinone scaffold,” Bioorganic & Medicinal Chemistry, vol. 17, no. 2, pp. 634–640, 2009.
View at: Publisher Site | Google Scholar
R. Beck, R. C. Pedrosa, N. Dejeans et al., “Ascorbate/menadione-induced oxidative stress kills cancer cells that express normal or mutated forms of the oncogenic protein Bcr-Abl. An in vitro and in vivo mechanistic study,” Investigational New Drugs, vol. 29, no. 5, pp. 891–900, 2011.
View at: Publisher Site | Google Scholar
M. Pardo, K. Joos, and W. Schäfer, “Über die oxidative Aminierung von 1,4-dihydroxy-2-acetonaphthon,” Liebigs Annalen der Chemie, vol. 1979, no. 4, pp. 503–521, 1979.
View at: Publisher Site | Google Scholar
W. Schäfer and H. Schlude, “Uber die chemie substituierter p-benzochinone IV synthese und eigenschaften substituierter aminochinone,” Tetrahedron Letters, vol. 8, no. 44, pp. 4307–4312, 1967.
View at: Publisher Site | Google Scholar
J. A. Valderrama, M. Cabrera, J. Benites et al., “Synthetic approaches and in vitro cytotoxic evaluation of 2-acyl-3-(3,4,5-trimethoxyanilino)-1,4-naphthoquinones,” RSC Advances, vol. 7, no. 40, pp. 24813–24821, 2017.
View at: Publisher Site | Google Scholar
J. Benites, H. Toledo, F. Salas et al., “In vitro inhibition of Helicobacter pylori growth by redox cycling phenylaminojuglones,” Oxidative Medicine and Cellular Longevity, vol. 2018, Article ID 1618051, 8 pages, 2018.
View at: Google Scholar
J. Benites, D. Rios, P. Díaz, and J. A. Valderrama, “The solar-chemical photo-Friedel–Crafts heteroacylation of 1,4-quinones,” Tetrahedron Letters, vol. 52, no. 5, pp. 609–611, 2011.
View at: Publisher Site | Google Scholar
T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays,” The Journal of Immunological Methods, vol. 65, no. 1-2, pp. 55–63, 1983.
View at: Publisher Site | Google Scholar
J. A. Valderrama, D. Ríos, G. G. Muccioli, P. Buc Calderon, and J. Benites, “In vitro inhibition of Hsp90 protein by benzothiazoloquinazolinequinones is enhanced in the presence of ascorbate. A preliminary in vivo antiproliferative study,” Molecules, vol. 25, no. 4, pp. 953–966, 2020.
View at: Publisher Site | Google Scholar
G. Bartoli, E. Marcantoni, M. Marcolini, and L. Sambri, “Applications of CeCl3as an environmental friendly promoter in organic chemistry,” Chemical Reviews, vol. 110, no. 10, pp. 6104–6143, 2010.
View at: Publisher Site | Google Scholar
A. Corma and H. Garcia, “Lewis acids: from conventional homogeneous to green homogeneous and heterogeneous catalysis,” Chemical Reviews, vol. 103, no. 11, pp. 4307–4366, 2003.
View at: Publisher Site | Google Scholar
M. Kidwai and A. Jahan, “Cerium chloride (CeCl3·7H2O) as a highly efficient catalyst for one-pot three-component Mannich reaction,” Journal of the Brazilian Chemical Society, vol. 21, no. 12, pp. 2175–2179, 2010.
View at: Publisher Site | Google Scholar
J. M. Dos Santos Filho, “Mild, stereoselective, and highly efficient synthesis ofN-acylhydrazones mediated by CeCl₃·7H₂O in a broad range of solvents,” European Journal of Organic Chemistry, vol. 2014, no. 29, pp. 6411–6417, 2014.
View at: Publisher Site | Google Scholar
E. Leyva, K. M. Baines, C. G. Espinosa-González et al., “2-(Fluoro-) and 2-(methoxyanilino)-1,4-naphthoquinones. Synthesis and mechanism and effect of fluorine substitution on redox reactivity and NMR,” Journal of Fluorine Chemistry, vol. 180, pp. 152–160, 2015.
View at: Publisher Site | Google Scholar
M. A. Brimble, O. Laita, and J. E. Robinson, “Addition of 2-tert-butyldimethylsilyloxythiophene to activated quinones: an approach to thia analogues of kalafungin,” Tetrahedron, vol. 62, no. 13, pp. 3021–3027, 2006.
View at: Publisher Site | Google Scholar
P. Ashok and A. Ilangovan, “Transition metal mediated selective C vs N arylation of 2-aminonaphthoquinone and its application toward the synthesis of benzocarbazoledione,” Tetrahedron Letters, vol. 59, no. 5, pp. 438–441, 2018.
View at: Publisher Site | Google Scholar
C. D. S. Lisboa, V. G. Santos, B. G. Vaz, N. C. de Lucas, M. N. Eberlin, and S. J. Garden, “C-H functionalization of 1,4-naphthoquinone by oxidative coupling with anilines in the presence of a catalytic quantity of copper(II) acetate,” The Journal of Organic Chemistry, vol. 76, pp. 5264–5273, 2011.
View at: Google Scholar
Y. T. Pratt, “Quinolinequinones. VI. Reactions with aromatic amines1,” The Journal of Organic Chemistry, vol. 27, no. 11, pp. 3905–3910, 1962.
View at: Publisher Site | Google Scholar
J. V. Schurman and E. I. Becker, “Synthesis of naphthylaminonaphthoquinones,” The Journal of Organic Chemistry, vol. 18, no. 2, pp. 211–217, 1953.
View at: Publisher Site | Google Scholar
J. A. Ibacache, J. A. Valderrama, J. Faúndes, A. Danimann, F. J. Recio, and C. A. Zúñiga, “Green synthesis and electrochemical properties of mono- and dimers derived from phenylaminoisoquinolinequinones,” Molecules, vol. 24, no. 23, pp. 4378–4390, 2019.
View at: Publisher Site | Google Scholar
B. Liu and S.-J. Ji, “Facile synthesis of 2‐amino‐1,4‐naphthoquinones catalyzed by molecular iodine under ultrasonic irradiation,” Synthetic Communications, vol. 38, no. 8, pp. 1201–1211, 2008.
View at: Publisher Site | Google Scholar
P. Ravichandiran, S. Sheet, D. Premnath, A. R. Kim, and D. J. Yoo, “1,4-naphthoquinone analogues: potent antibacterial agents and mode of action evaluation,” Molecules, vol. 24, no. 7, pp. 1437–1451, 2019.
View at: Publisher Site | Google Scholar
P. Ravichandiran, M. Masłyk, S. Sheet et al., “Synthesis and antimicrobial evaluation of 1,4-naphthoquinone derivatives as potential antibacterial agents,” ChemistryOpen, vol. 8, no. 5, pp. 589–600, 2019.
View at: Publisher Site | Google Scholar
D. J. Yoo, P. Ravichandiran, S. A. Subramaniyan et al. et al., “Synthesis anticancer evaluation of novel 1,4-naphthoquinone derivatives containing a phenylamino-sulfanyl moiety,” ChemMedChem, vol. 14, no. 5, pp. 532–544, 2019.
View at: Google Scholar
C. Glorieux and P. Buc Calderon, “Cancer cell sensitivity to redox-cycling quinones is influenced by NAD(P)H: quinone oxidoreductase 1 polymorphism,” Antioxidants, vol. 8, no. 9, pp. 369–378, 2019.
View at: Publisher Site | Google Scholar

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Copyright © 2020 David Ríos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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