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PPAR Research
Volume 2010, Article ID 679184, 17 pages
http://dx.doi.org/10.1155/2010/679184
Research Article

Coordinate Transcriptomic and Metabolomic Effects of the Insulin Sensitizer Rosiglitazone on Fundamental Metabolic Pathways in Liver, Soleus Muscle, and Adipose Tissue in Diabetic db/db Mice

1Division of Molecular Pharmacology and Pathophysiology, Institut de Recherches Servier, 125 Chemin de Ronde, 78290 Croissy-sur-Seine, France
2Department of Biostatistics, Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France
3Metabolisme BA, Technologie Servier, 5 rue Bel Air, 45000 Orleans, France
4Division of Diabetes Metabolic Diseases Research, Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France

Received 29 April 2010; Revised 16 July 2010; Accepted 11 August 2010

Academic Editor: Chih-Hao Lee

Copyright © 2010 Sabrina Le Bouter et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. Jiang, Q. Dallas-Yang, and Q. Dallas-Yang, “Potentiation of insulin signaling in tissues of Zucker obese rats after acute and long-term treatment with PPARγ agonists,” Diabetes, vol. 51, no. 8, pp. 2412–2419, 2002. View at Google Scholar · View at Scopus
  2. A. B. Mayerson, R. S. Hundal, and R. S. Hundal, “The effects of rosiglitazone on insulin sensitivity, lipolysis, and hepatic and skeletal muscle triglyceride content in patients with type 2 diabetes,” Diabetes, vol. 51, no. 3, pp. 797–802, 2002. View at Google Scholar · View at Scopus
  3. A. M. Sharma and B. Staels, “Review: peroxisome proliferator-activated receptor γ and adipose tissue—understanding obesity-related changes in regulation of lipid and glucose metabolism,” Journal of Clinical Endocrinology and Metabolism, vol. 92, no. 2, pp. 386–395, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  4. C. Tiraby, G. Tavernier, C. Lefort, D. Larrouy, F. Bouillaud, D. Ricquier, and D. Langin, “Acquirement of brown fat cell features by human white adipocytes,” Journal of Biological Chemistry, vol. 278, no. 35, pp. 33370–33376, 2003. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  5. D. L. Gerhold, F. Liu, and F. Liu, “Gene expression profile of adipocyte differentiation and its regulation by peroxisome proliferator-activated receptor-γ agonists,” Endocrinology, vol. 143, no. 6, pp. 2106–2118, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. L. Teboul, D. Gaillard, L. Staccini, H. Inadera, E.-Z. Amri, and P. A. Grimaldi, “Thiazolidinediones and fatty acids convert myogenic cells into adipose-like cells,” Journal of Biological Chemistry, vol. 270, no. 47, pp. 28183–28187, 1995. View at Publisher · View at Google Scholar · View at Scopus
  7. P. Tontonoz, E. Hu, R. A. Graves, A. I. Budavari, and B. M. Spiegelman, “mPPARγ2: tissue-specific regulator of an adipocyte enhancer,” Genes and Development, vol. 8, no. 10, pp. 1224–1234, 1994. View at Google Scholar · View at Scopus
  8. S. Hallakou, L. Doaré, and L. Doaré, “Pioglitazone induces in vivo adipocyte differentiation in the obese Zucker fa/fa rat,” Diabetes, vol. 46, no. 9, pp. 1393–1399, 1997. View at Google Scholar · View at Scopus
  9. Y. Zhu, K. Alvares, Q. Huang, M. S. Rao, and J. K. Reddy, “Cloning of a new member of the peroxisome proliferator-activated receptor gene family from mouse liver,” Journal of Biological Chemistry, vol. 268, no. 36, pp. 26817–26820, 1993. View at Google Scholar · View at Scopus
  10. A. Gastaldelli, Y. Miyazaki, M. Pettiti, E. Santini, D. Ciociaro, R. A. DeFronzo, and E. Ferrannini, “The effect of rosiglitazone on the liver: decreased gluconeogenesis in patients with type 2 diabetes,” Journal of Clinical Endocrinology and Metabolism, vol. 91, no. 3, pp. 806–812, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  11. C. J. de Souza, M. Eckhardt, K. Gagen, M. Dong, W. Chen, D. Laurent, and B. F. Burkey, “Effects of pioglitazone on adipose tissue remodeling within the setting of obesity and insulin resistance,” Diabetes, vol. 50, no. 8, pp. 1863–1871, 2001. View at Google Scholar · View at Scopus
  12. I. García-Ruiz, C. Rodríguez-Juan, T. Díaz-Sanjuán, M. Á. Martínez, T. Muñoz-Yagüe, and J. A. Solís-Herruzo, “Effects of rosiglitazone on the liver histology and mitochondrial function in ob/ob mice,” Hepatology, vol. 46, no. 2, pp. 414–423, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  13. S. M. Watkins, P. R. Reifsnyder, H.-J. Pan, J. B. German, and E. H. Leiter, “Lipid metabolome-wide effects of the PPARγ agonist rosiglitazone,” Journal of Lipid Research, vol. 43, no. 11, pp. 1809–1817, 2002. View at Publisher · View at Google Scholar · View at Scopus
  14. P. Wang, J. Renes, F. Bouwman, A. Bunschoten, E. Mariman, and J. Keijer, “Absence of an adipogenic effect of rosiglitazone on mature 3T3-L1 adipocytes: increase of lipid catabolism and reduction of adipokine expression,” Diabetologia, vol. 50, no. 3, pp. 654–665, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  15. M. Loffler, M. Bilban, M. Reimers, W. Waldhäusl, and T. M. Stulnig, “Blood glucose-lowering nuclear receptor agonists only partially normalize hepatic gene expression in db/db mice,” Journal of Pharmacology and Experimental Therapeutics, vol. 316, no. 2, pp. 797–804, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  16. J. M. Way, W. W. Harrington, and W. W. Harrington, “Comprehensive messenger ribonucleic acid profiling reveals that peroxisome proliferator-activated receptor γ activation has coordinate effects on gene expression in multiple insulin-sensitive tissues,” Endocrinology, vol. 142, no. 3, pp. 1269–1277, 2001. View at Publisher · View at Google Scholar · View at Scopus
  17. A. Suzuki, T. Yasuno, H. Kojo, J. Hirosumi, S. Mutoh, and Y. Notsu, “Alteration in expression profiles of a series of diabetes-related genes in db/db mice following treatment with thiazolidinediones,” Japanese Journal of Pharmacology, vol. 84, no. 2, pp. 113–123, 2000. View at Publisher · View at Google Scholar · View at Scopus
  18. K. Kobayashi, T. M. Forte, S. Taniguchi, B. Y. Ishida, K. Oka, and L. Chan, “The db/db mouse, a model for diabetic dyslipidemia: molecular characterization and effects of western diet feeding,” Metabolism: Clinical and Experimental, vol. 49, no. 1, pp. 22–31, 2000. View at Google Scholar · View at Scopus
  19. L. Weng, H. Dai, Y. Zhan, Y. He, S. B. Stepaniants, and D. E. Bassett, “Rosetta error model for gene expression analysis,” Bioinformatics, vol. 22, no. 9, pp. 1111–1121, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  20. K. Cartharius, K. Frech, and K. Frech, “MatInspector and beyond: promoter analysis based on transcription factor binding sites,” Bioinformatics, vol. 21, no. 13, pp. 2933–2942, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  21. S. Wold, M. Sjöström, and L. Eriksson, “PLS-regression: a basic tool of chemometrics,” Chemometrics and Intelligent Laboratory Systems, vol. 58, no. 2, pp. 109–130, 2001. View at Publisher · View at Google Scholar · View at Scopus
  22. K. J. Livak and T. D. Schmittgen, “Analysis of relative gene expression data using real-time quantitative PCR and the 2ΔΔCT method,” Methods, vol. 25, no. 4, pp. 402–408, 2001. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  23. H.-J. Choo, J.-H. Kim, and J.-H. Kim, “Mitochondria are impaired in the adipocytes of type 2 diabetic mice,” Diabetologia, vol. 49, no. 4, pp. 784–791, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  24. J. X. Rong, Y. Qiu, and Y. Qiu, “Adipose mitochondrial biogenesis is suppressed in db/db and high-fat diet-fed mice and improved by rosiglitazone,” Diabetes, vol. 56, no. 7, pp. 1751–1760, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  25. T. M. Chan, K. M. Young, N. J. Hutson, F. T. Brumley, and J. H. Exton, “Hepatic metabolism of genetically diabetic (db/db) mice. I. Carbohydrate metabolism,” American Journal of Physiology, vol. 229, no. 6, pp. 1702–1712, 1975. View at Google Scholar · View at Scopus
  26. G. Boden, X. Chen, and T. P. Stein, “Gluconeogenesis in moderately and severely hyperglycemic patients with type 2 diabetes mellitus,” American Journal of Physiology, vol. 280, no. 1, pp. E23–E30, 2001. View at Google Scholar · View at Scopus
  27. B. A. Neuschwander-Tetri, E. M. Brunt, K. R. Wehmeier, C. A. Sponseller, K. Hampton, and B. R. Bacon, “Interim results of a pilot study demonstrating the early effects of the PPAR-γ ligand rosiglitazone on insulin sensitivity, aminotransferases, hepatic steatosis and body weight in patients with non-alcoholic steatohepatitis,” Journal of Hepatology, vol. 38, no. 4, pp. 434–440, 2003. View at Publisher · View at Google Scholar · View at Scopus
  28. W.-N. Cong, R.-Y. Tao, J.-Y. Tian, G.-T. Liu, and F. Ye, “The establishment of a novel non-alcoholic steatohepatitis model accompanied with obesity and insulin resistance in mice,” Life Sciences, vol. 82, no. 19-20, pp. 983–990, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  29. S. M. Cohen, J. G. Werrmann, and M. R. Tota, “13C NMR study of the effects of leptin treatment on kinetics of hepatic intermediary metabolism,” Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 13, pp. 7385–7390, 1998. View at Google Scholar · View at Scopus
  30. P. Cohen, M. Miyazaki, and M. Miyazaki, “Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss,” Science, vol. 297, no. 5579, pp. 240–243, 2002. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  31. J. D. Horton, J. L. Goldstein, and M. S. Brown, “SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver,” Journal of Clinical Investigation, vol. 109, no. 9, pp. 1125–1131, 2002. View at Publisher · View at Google Scholar · View at Scopus
  32. N. Gallardo, E. Bonzón-Kulichenko, and E. Bonzón-Kulichenko, “Tissue-specific effects of central leptin on the expression of genes involved in lipid metabolism in liver and white adipose tissue,” Endocrinology, vol. 148, no. 12, pp. 5604–5610, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  33. L. Jiang, Q. Wang, and Q. Wang, “Leptin contributes to the adaptive responses of mice to high-fat diet intake through suppressing the lipogenic pathway,” PLoS One, vol. 4, no. 9, Article ID e6884, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  34. M. Guerro-Millo, “Adipose tissue and adipokines: for better or worse,” Diabetes and Metabolism, vol. 30, no. 1, pp. 13–19, 2004. View at Google Scholar · View at Scopus
  35. Z. Wu, P. Puigserver, and P. Puigserver, “Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1,” Cell, vol. 98, no. 1, pp. 115–124, 1999. View at Publisher · View at Google Scholar · View at Scopus
  36. C. D. N. Toseland, S. Campbell, I. Francis, P. J. Bugelski, and N. Mehdi, “Comparison of adipose tissue changes following administration of rosiglitazone in the dog and rat,” Diabetes, Obesity and Metabolism, vol. 3, no. 3, pp. 163–170, 2001. View at Publisher · View at Google Scholar · View at Scopus
  37. M. Adams, C. T. Montague, and C. T. Montague, “Activators of peroxisome proliferator-activated receptor γ have depot- specific effects on human preadipocyte differentiation,” Journal of Clinical Investigation, vol. 100, no. 12, pp. 3149–3153, 1997. View at Google Scholar · View at Scopus
  38. Y. Miyazaki, H. He, L. J. Mandarino, and R. A. DeFronzo, “Rosiglitazone improves downstream insulin receptor signaling in type 2 diabetic patients,” Diabetes, vol. 52, no. 8, pp. 1943–1950, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. M. L. Paddock, S. E. Wiley, and S. E. Wiley, “MitoNEET is a uniquely folded 2Fe-2S outer mitochondrial membrane protein stabilized by pioglitazone,” Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 36, pp. 14342–14347, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus