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Stem Cells International
Volume 2016 (2016), Article ID 6759343, 7 pages
Research Article

TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells

1Institute of Neuroanatomy, Eberhard Karls University Tübingen, 72074 Tübingen, Germany
2Department of Internal Medicine I, Ulm University, 89081 Ulm, Germany

Received 24 April 2015; Accepted 2 July 2015

Academic Editor: Su-Chun Zhang

Copyright © 2016 Moritz Klingenstein et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


TBX3 is a member of the T-box transcription factor family and is involved in the core pluripotency network. Despite this role in the pluripotency network, its contribution to the reprogramming process during the generation of human induced pluripotent stem cells remains elusive. In this respect, we performed reprogramming experiments applying TBX3 knockdown in human fibroblasts and keratinocytes. Knockdown of TBX3 in both somatic cell types decreased the reprogramming efficiencies in comparison to control cells but with unchanged reprogramming kinetics. The resulting iPSCs were indistinguishable from control cells and displayed a normal in vitro differentiation capacity by generating cells of all three germ layers comparable to the controls.