Rapid Identification of Chemical Constituents in <i>Hericium erinaceus</i> Based on LC-MS/MS Metabolomics

Yang, Fei; Wang, Honglin; Feng, Guoquan; Zhang, Sulan; Wang, Jinmei; Cui, Lili

doi:https://doi.org/10.1155/2021/5560626

Journal of Food Quality

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Abstract Introduction Materials and Methods Results Discussion and Conclusion Data Availability Conflicts of Interest Acknowledgments References Copyright Related Articles

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Applications of Mass Spectrometry in the Analysis of Food Composition and Contaminants

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Research Article | Open Access

Volume 2021 | Article ID 5560626 | https://doi.org/10.1155/2021/5560626

Rapid Identification of Chemical Constituents in Hericium erinaceus Based on LC-MS/MS Metabolomics

Fei Yang,^1,2Honglin Wang,¹Guoquan Feng,³Sulan Zhang,⁴Jinmei Wang,¹and Lili Cui¹

Academic Editor: Xiao-zhi Tang

Received19 Feb 2021

Revised14 Mar 2021

Accepted22 Mar 2021

Published29 Mar 2021

Abstract

Hericium erinaceus is a precious edible and medicinal fungus with high nutritional value. It has many functions, such as enhancing immunity, antitumor antioxidation, antihyperglycemic, antihyperlipidemic, and protecting gastric mucosa. However, there are few researches about the H. erinaceus compounds. In this paper, ultraperformance liquid chromatography tandem high-resolution mass spectrometry (UPLC-Q-exactive-MS/MS) was used to isolate and identify the compounds in H. erinaceus. 102 compounds were identified in H. erinaceus by comparing with standard databases such as MZVault, MZCloud, and BGI Library (self-built standard Library by BGI Co., Ltd), including flavonoids, terpenoids, phenolic acids, phenylpropanoids, steroids, organic acids, and amino acids.

1. Introduction

Hericium erinaceus is a precious edible and medicinal fungus, and it is listed as one of the “Four Famous Cuisines” of China, together with bear’s paws, trepang, and shark’s fin [1]; it has been used for a long time in traditional Chinese medicine [2]. Researches showed that the chemical constituents of H. erinaceus include terpenoids, phenolics, steroids, pyranones, fatty acids, and alkaloids; about 80 small molecular compounds were isolated and identified from H. erinaceus [3]. Terpenoids in H. erinaceus were mainly diterpenoids with cyathane skeleton. Terpenoids in H. erinaceus were first isolated and identified by Kawagishi et al. from mycelia of H. erinaceus, named Erinacines A-C [4]. Subsequently, Kawagishi et al. isolated and identified Erinacines D-G, Erinacines J, and Erinacines K from mycelia of H. erinaceus [5–7]; Lee et al. isolated and identified Erinacines H and Erinacines I from mycelia of H. erinaceus [8]; Kenmoku et al. isolated and identified Erinacine P and Erinacine Q from mycelia of H. erinaceus [9, 10]. Most of these diterpenoids compounds were stimulators of nerve growth factor-synthesis. Phenolics were also main constituents in H. erinaceus. 8 phenolics were isolated and identified from fruiting bodies of H. erinaceus by the Kawagishi team between 1990 and 1993, named Hericenone A-H [11–13]. After that, three phenolics were isolated and identified by Arnone et al. [14] with 5′- carbonyl group replaced by 5′- methylene, named Hericenes A-C. Subsequently, two new phenolics were isolated from fruiting bodies of H. erinaceus by Ma et al. [15], named Hericenone I and Hericenone D; they have the same fatty acid chain. A new skeleton phenolic compound was isolated from fruiting bodies of H. erinaceus by Li et al. [16], named Erinacene D. In addition to terpenoids and phenolics compounds, H. erinaceus was rich in steroids, six new (erinarols A-F), and five known ergostane-type sterol fatty acid esters were isolated from the fruiting bodies of H. erinaceus; erinarols A and B significantly activated the transcriptional activity of PPARs, PPARα, and PPARγ [17]. Previous pharmacological studies showed that H. erinaceus had many pharmacological activities, such as regulating immunity [18, 19], neuroprotection [20, 21], antidepressant [22, 23], antioxidant [24], antitumor [25], antihyperglycemic [26], and antihyperlipidemic properties [27], and protecting gastric mucosa [28, 29].

High-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) technology has the advantage of rapid identification of compounds. With the maturity of LC-MS/MS technology, it is more and more widely used in the field of food and medicine [30–35]. In this study, ultraperformance liquid chromatography tandem high-resolution mass spectrometry (UPLC-Q-Exactive-MS/MS) combined with standard substance database was used to rapidly identify the chemical components of H. erinaceus.

2. Materials and Methods

2.1. Instrument

The LC-MS experiment was carried out by ultraperformance liquid chromatography (Waters 2D UPLC, USA) and high-resolution mass spectrometry (Q Exactive, Thermo, USA). The Hypersil GOLD aQ column (100 mm2.1 mm, 1.9 μm) was purchased from Thermo Fisher Scientific, USA. Low-temperature high-speed centrifuge (Centrifuge 5430) was purchased from Eppendorf. The vortex finder (QL-901) was purchased from Qilinbeier Instrument Manufacturing Co., Ltd. The pure water meter (Milli-Q) was purchased from Integral Millipore Corporation, USA.

2.2. Reagent

d₃-leucine, ¹³C₉-phenylalanine, d₅-tryptophan, and ¹³C₃-progesterone were used as internal standards. Methanol (A454-4) and acetonitrile (A996-4) were both in mass spectral grade, which were purchased from Thermo Fisher Scientific, USA. Ammonium formate (17843–250 G) was obtained from Honeywell Fluka, USA. Formic acid (50144–50 mL) was obtained from DIMKA, USA.

2.3. Materials

Fruiting bodies of H. erinaceus were obtained from Henan Longfeng Industrial Co., Ltd. The specimens (No. 2020-09-09) were saved in National Research and Development Center of Edible Fungi Processing Technology, Henan University.

2.4. Preparation of Sample

According to the methods commonly used by our team, dry fruiting bodies of H. erinaceus were crushed by the grinding machine. 100 g of H. erinaceus powder was immersed with 50% ethanol (1000 mL) for 2 times at room temperature, each time for 3 days. The filtrate was concentrated to obtain 48.6 g extract. 50 mg extract of H. erinaceus was weighed, and then 800 μL of solution with 70% methanol: internal standard ( = 85 : 1) was added to the sample. Two small steel beads were added to the sample and ground in a tissue grinder (50 Hz, 5 min), ultrasound for 10 min at 4°C water, and the sample was placed for 1 h at −20°C. After that, the sample was centrifuged for 15 min at 4°C, 25000 rpm, 500 μL of supernatant was filtered through 96-well filter plate, and the filtrate was used to detect.

2.5. Chromatographic Conditions

The LC-MS experiment was carried out on Hypersil GOLD a Q column (100 mm2.1 mm, 1.9 μm). The mobile phase was 0.1% formic acid-water (liquid A) and 0.1% formic acid-acetonitrile (liquid B). The elution gradient was as follows: 0–2 min 5% B; 2–22 min 5%–95% B; 22–27 min 95% B; 27.1–30 min 5% B. The flow rate was 0.3 mL/min, the column temperature was 40°C, and the injection volume was 5 μL.

2.6. Mass Spectrometry Conditions

The MS and MS² data were collected by the Q Exactive mass spectrometer. The range of m/z was 150–1500 with the MS resolution 70000, and the AGC was 1 e⁶, and the maximum injection time was 100 ms. According to the strength of the MS ions, top3 was selected for determining MS² fragmentation. The MS² resolution was 35000, AGC was 2 e⁵, the maximum injection time was 50 ms, and the fragmentation energy was set as 20, 40, and 60 eV. Ion source (ESI) parameters are as follows: sheath gas flow rate was 40, aux gas flow rate was 10, spray voltage (|KV|) of positive ion mode was 3.80, spray voltage (|KV|) of negative ion mode was 3.20, capillary temp was 320°C, and aux gas heater temp was 350°C.

2.7. Data Analysis

High-resolution mass spectrometer (Q Exactive, Thermo Fisher Scientific, USA) was used to collect data in positive and negative ion modes, respectively, to improve the chemical constituent coverage. Raw mass spectrum data collected by LC-MS/MS were imported into Compound Discoverer 3.1 (Thermo Fisher Scientific, USA) for data processing. It mainly includes peak extraction, retention time correction within and between groups, additive ion merging, missing value filling, background peak labeling, and metabolite identification. Finally, the molecular weight, retention time, peak area, and identification results of the compound were derived.

3. Results

3.1. Total Ion Chromatogram

The total ion chromatogram of H. erinaceus is shown in Figure 1.

3.2. Results of Compound Identification

The compounds of H. erinaceus were analyzed by LC-MS/MS; the identification of structure was based on the retention time, MS data, and MS² data compared with the standard database. The identified compounds were classified into three grades (Level 1, Level 2, and Level 3) according to the comparison results, there into, Level 2 was confirmed on the basis of MS data, MS² data, and properties of compounds, and Level 3 was identified on the basis of MS data and MS² data. The credibility sequence is as follows: Level 1> Level 2> Level 3. Detailed results are shown in Table 1. 102 compounds were preliminarily identified in H. erinaceus with grade of identification as Level 1 and Level 2, including 31 organic acids, 10 nucleotides and analogues, 8 amino acids, 6 carbohydrates and derivatives, 5 flavonoids, 3 unsaturated fatty acids, 3 terpenoids, 3 phenolic acids, 1 phenylpropanoid, 1 steroid, and 32 other compounds. Most flavonoids and organic acids were easily deprotonated and responded in the negative ion mode. Most nucleoside compounds were easily protonated and responded in the positive ion mode. The identification process of some compounds was as follows.

Compound 49, C₁₅H₁₀O₆, was easily deprotonated in the negative ion mode to produce ion m/z 285 [M − H]⁻ and then RDA cracking to get 2 fragment ions m/z 133 [C₈H₆O₂ – H]⁻ and m/z 151 [C₇H₄O₄ – H]⁻. Compound 49 was identified as luteolin compared to the database and references [36]. The MS² spectrum is shown in Figure 2.

Compound 60 produced ion m/z 283 [M − H]⁻, then lost the methyl group, and got ion 268 [M − H − CH₃]⁻, combined with retention time, MS data, and MS² data, compound 60 was identified as acacetin by comparin with the database and references [37]. The MS² spectrum is shown in Figure 3.

Compound 62 was easily protonated in the positive ion mode to produce ion m/z 249 [M + H]⁺, then lost the hydroxyl group, and got ion m/z 231 [M + H – H₂O]⁺, Compound 62 was identified as atractylenolide III by comparing with the database and references [38]. The MS² spectrum is shown in Figure 4.

4. Discussion and Conclusion

In this study, 102 compounds were preliminarily identified in H. erinaceus, including organic acids, nucleotides and analogues, amino acids, carbohydrates and derivatives, flavonoids, unsaturated fatty acids, terpenoids, phenolic acids, phenylpropanoid, and steroid. It revealed that the compounds in H. erinaceus were diverse. Previous studies showed that the chemical constituents of H. erinaceus include terpenoids, phenolics, steroids, pyranones, fatty acids, and alkaloids; thereinto, reports about terpenoids and phenolics in H. erinaceus were more [3–16]. Terpenoids and phenolics were less in this study; the reasons may be different material parts, different origins, different varieties, different extraction methods, and so on. In addition, nucleotides were also the main constituents in H. erinaceus. Yan et al. [39] studied the content of five nucleosides in H. erinaceus from different habitats by high-performance liquid chromatography; the results showed that different origins of H. erinaceus had the same nucleoside components, such as cytosine, inosine, and adenosine, and the content of inosine and adenosine in H. erinaceus was higher. In this study, 10 nucleotides and analogues were identified, including adenosine, adenine, guanosine, guanine, and uridine.

Most of the compounds in H. erinaceus had a good biological activity; researches about polysaccharide were more, which had a wide variety of pharmacological functions such as antimicrobial, antidiabetic, and antihypertension ones [1]. Small molecular compounds isolated from H. erinaceus also had many biological activities. Diterpenoids in H. erinaceus could promote the synthesis of nerve growth factors, such as Erinacines A, Erinacines B, Erinacines C, Erinacines D, Erinacines E, Erinacines F, Erinacines G, Erinacines H, and Erinacines I [4–6]. Phenolics compounds in H. erinaceus could also promote the synthesis of nerve growth factors, such as Hericenone C, Hericenone D, Hericenone E, and Hericenone H [12, 13]. Hericenones A and B showed cytotoxicity against HeLa cells [11]. In addition, Hericenone B was found to be a potential antiplatelet aggregation agent [40]. Steroids in H. erinaceus could activate the transcriptional activity of PPARs, PPARα, and PPARγ, such as erinarols A and erinarols B. In addition, nucleosides and flavonoids may be the main active components of H. erinaceus. Nucleoside components have many biological activities, such as antitumor, antivirus, and gene therapy. Studies have shown that adenosine, inosine, and guanosine have many pharmacological effects such as regulating immunity, neuroprotection, and treatment of cardiovascular diseases [41]. Flavonoids also have many pharmacological effects, such as neuroprotection, antimyocardial ischemia, hypotension, improved learning and memory, antigastric ulcer, protection of reproductive tissue, anti-inflammatory, and antitumor [42].

In a word, H. erinaceus has high nutritional value and medicinal value. At present, it has been developed into a variety of functional foods, including health wine, health drinks, healthy yogurt, tea, cans, and health vinegar [2], which was of great significance to study the chemical composition of H. erinaceus. In this study, 102 compounds were preliminarily identified to provide reference for the follow-up study of H. erinaceus.

Data Availability

The data used to support this study are available within the article.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding this study.

Acknowledgments

This work was supported by the National Key R&D Program of China (2018YFD0400200), the Major Public Welfare Projects in Henan Province (201300110200), and the Key Project in Science and Technology Agency of Henan Province (192102110214 and 202102110283).

References

M. Wang, Y. Gao, D. Xu, T. Konishi, and Q. Gao, “Hericium erinaceus (Yamabushitake): a unique resource for developing functional foods and medicines,” Food & Function, vol. 5, no. 12, pp. 3055–3064, 2014.
View at: Publisher Site | Google Scholar
J. Y. Tan, J. J. Wang, X. L. Wang et al., “The health value of Hericium erinaceus (Review),” Edible and Medicinal Mushroom, vol. 23, no. 3, pp. 188–193, 2015.
View at: Google Scholar
Y. Zhang, Chemical Constituents of Hericium erinaceus, Northwest A ＆ F University, Xianyang, China, 2016.
H. Kawagishi, A. Shimada, R. Shirai et al., “Erinacines A, B and C, strong stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum,” Tetrahedron Letters, vol. 35, no. 10, pp. 1569–1572, 1994.
View at: Publisher Site | Google Scholar
H. Kawagishi, A. Simada, K. Shizuki et al., “Erinacine D, a stimulator of NGF-synthesis, from the mycelia of Hericium erinaceum,” Heterocyclic Communications, vol. 2, no. 1, pp. 51–54, 1996.
View at: Publisher Site | Google Scholar
H. Kawagishi, A. Shimada, S. Hosokawa et al., “Erinacines E, F, and G, stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum,” Tetrahedron Letters, vol. 37, no. 41, pp. 7399–7402, 1996.
View at: Publisher Site | Google Scholar
H. Kawagishi, A. Masui, S. Tokuyama, and T. Nakamura, “Erinacines J and K from the mycelia of Hericium erinaceum,” Tetrahedron, vol. 62, no. 36, pp. 8463–8466, 2006.
View at: Publisher Site | Google Scholar
E. W. Lee, K. Shizuki, S. Hosokawa et al., “Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum,” Bioscience, Biotechnology, and Biochemistry, vol. 64, no. 11, pp. 2402–2405, 2000.
View at: Publisher Site | Google Scholar
H. Kenmoku, T. Sassa, and N. Kato, “Isolation of erinacine P, a new parental metabolite of cyathane-xylosides, from Hericium erinaceum and its biomimetic conversion into erinacines A and B,” Tetrahedron Letters, vol. 41, no. 22, pp. 4389–4393, 2000.
View at: Publisher Site | Google Scholar
H. Kenmoku, T. Shimai, T. Toyomasu, N. Kato, and T. Sassa, “Erinacine Q, a new erinacine from Hericium erinaceum, and its biosynthetic route to erinacine C in the basidiomycete,” Bioscience, Biotechnology, and Biochemistry, vol. 66, no. 3, pp. 571–575, 2002.
View at: Publisher Site | Google Scholar
H. Kawagishi, M. Ando, and T. Mizuno, “Hericenone A and B as cytotoxic principles from the mushroom,” Tetrahedron Letters, vol. 31, no. 3, pp. 373–376, 1990.
View at: Publisher Site | Google Scholar
H. Kawagishi, M. Ando, H. Sakamoto et al., “Hericenones C, D and E, stimulators of nerve growth factor (NGF)-synthesis, from the mushroom Hericium erinaceum,” Tetrahedron Letters, vol. 32, no. 35, pp. 4561–4564, 1991.
View at: Publisher Site | Google Scholar
H. Kawagishi, M. Ando, and K. Shinba, “Chromans, hericenones F, G and H from the mushroom of Hericium erinaceum,” Phytochemistry, vol. 32, no. 1, pp. 175–178, 1993.
View at: Google Scholar
A. Arnone, R. Cardillo, G. Nasini, and O. V. de Pava, “Secondary mold metabolites: part 46. hericenes A-C and erinapyrone C, new metabolites produced by the fungus Hericium erinaceus,” Journal of Natural Products, vol. 57, no. 5, pp. 602–606, 1994.
View at: Publisher Site | Google Scholar
B.-J. Ma, H.-Y. Yu, J.-W. Shen et al., “Cytotoxic aromatic compounds from Hericium erinaceum,” The Journal of Antibiotics, vol. 63, no. 12, pp. 713–715, 2010.
View at: Publisher Site | Google Scholar
W. Li, Y. N. Sun, W. Zhou, S. H. Shim, and Y. H. Kim, “Erinacene D, a new aromatic compound from Hericium erinaceum,” The Journal of Antibiotics, vol. 67, no. 10, pp. 727–729, 2014.
View at: Publisher Site | Google Scholar
W. Li, W. Zhou, S. B. Song, S. H. Shim, and Y. H. Kim, “Sterol fatty acid esters from the mushroom Hericium erinaceum and their PPAR transactivational effects,” Journal of Natural Products, vol. 77, no. 12, pp. 2611–2618, 2014.
View at: Publisher Site | Google Scholar
Z. Liu, L. Liao, Q. Chen et al., “Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in muscovy duck reovirus-infected ducklings,” International Journal of Biological Macromolecules, vol. 171, pp. 448–456, 2021.
View at: Publisher Site | Google Scholar
F. Wu and H. Huang, “Surface morphology and protective effect of Hericium erinaceus polysaccharide on cyclophosphamide-induced immunosuppression in mice,” Carbohydrate Polymers, vol. 251, p. 116930, 2021.
View at: Publisher Site | Google Scholar
S. Y. Lew, S. H. Lim, L. W. Lim et al., “Neuroprotective effects of Hericium erinaceus (Bull.: Fr.) Pers. against high-dose corticosterone-induced oxidative stress in PC-12 cells,” BMC Complementary Medicine and Therapies, vol. 20, no. 1, p. 340, 2020.
View at: Publisher Site | Google Scholar
P. S. Chong, S. Khairuddin, A. C. K. Tse et al., “Hericium erinaceus potentially rescues behavioural motor deficits through ERK-CREB-PSD95 neuroprotective mechanisms in rat model of 3-acetylpyridine-induced cerebellar ataxia,” Scientific Reports, vol. 10, no. 1, p. 14945, 2020.
View at: Publisher Site | Google Scholar
F. Limanaqi, F. Biagioni, C. L. Busceti, M. Polzella, C. Fabrizi, and F. Fornai, “Potential antidepressant effects of scutellaria baicalensis, Hericium erinaceus and Rhodiola Rosea,” Antioxidants (Basel, Switzerland), vol. 9, no. 3, p. 234, 2020.
View at: Publisher Site | Google Scholar
P. S. Chong, M.-L. Fung, K. H. Wong, and L. W. Lim, “Therapeutic potential of Hericium erinaceus for depressive disorder,” International Journal of Molecular Sciences, vol. 21, no. 1, p. 163, 2019.
View at: Publisher Site | Google Scholar
T. Qin, X. Liu, Y. Luo et al., “Characterization of polysaccharides isolated from Hericium erinaceus and their protective effects on the DON-induced oxidative stress,” International Journal of Biological Macromolecules, vol. 152, pp. 1265–1273, 2020.
View at: Publisher Site | Google Scholar
W. Li, W. Zhou, E.-J. Kim, S. H. Shim, H. K. Kang, and Y. H. Kim, “Isolation and identification of aromatic compounds in lion’s mane mushroom and their anticancer activities,” Food Chemistry, vol. 170, pp. 336–342, 2015.
View at: Publisher Site | Google Scholar
B. Liang, Z. D. Guo, F. Xie, and F. Zhao, “Antihyperglycemic and antihyperlipidemic activities of aqueous extract of Hericium erinaceus in experimental diabetic rats,” BioMed Central, vol. 13, no. 1, p. 253, 2013.
View at: Publisher Site | Google Scholar
K. Hiwatashi, Y. Kosaka, N. Suzuki et al., “Yamabushitake mushroom (Hericium erinaceus) improved lipid metabolism in mice fed a high-fat diet,” Bioscience, Biotechnology, and Biochemistry, vol. 74, no. 7, pp. 1447–1451, 2014.
View at: Publisher Site | Google Scholar
E. D. Yuan, M. Huang, L. Li et al., “Protective effect of Hericium erinaceus mycelium/fruit body polysaccharide on gastric mucosa,” Journal of Chinese Institute of Food Science and Technology, vol. 20, no. 11, pp. 71–78, 2020.
View at: Google Scholar
W. Chen, D. Wu, Y. Jin et al., “Pre-protective effect of polysaccharides purified from Hericium erinaceus against ethanol-induced gastric mucosal injury in rats,” International Journal of Biological Macromolecules, vol. 159, pp. 948–956, 2020.
View at: Publisher Site | Google Scholar
M. Hamza, E. Imane, A. Amal et al., “Antioxidant, anti-inflammatory and antidiabetic proprieties of LC-MS/MS identified polyphenols from coriander seeds,” Molecules, vol. 26, no. 2, p. 487, 2021.
View at: Publisher Site | Google Scholar
Q. Y. Zhang, X. J. Wang, X. M. Wang et al., “Development of a pass-through SPE cartridge for the rapid determination of fipronil and its metabolites in chicken eggs by LC-MS/MS,” Food Analytical Methods, 2021.
View at: Publisher Site | Google Scholar
W. Li, Y. Zhang, S. Shi et al., “Spectrum-effect relationship of antioxidant and tyrosinase activity with malus pumila flowers by UPLC-MS/MS and component knock-out method,” Food and Chemical Toxicology, vol. 133, p. 110754, 2019.
View at: Publisher Site | Google Scholar
J. Ma, S. Fan, L. Sun, L. He, Y. Zhang, and Q. Li, “Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography-tandem mass spectrometry,” Food Science and Human Wellness, vol. 9, no. 4, pp. 363–369, 2020.
View at: Publisher Site | Google Scholar
S. Fan, J. Ma, M. Cao et al., “Simultaneous determination of 15 pesticide residues in Chinese cabbage and cucumber by liquid chromatography-tandem mass spectrometry utilizing online turbulent flow chromatography,” Food Science and Human Wellness, vol. 10, no. 1, pp. 78–86, 2021.
View at: Publisher Site | Google Scholar
J. M. Ma, Q. Li, S. F. Fan et al., “Determination of 8 endogenous alkaloid components in boletus using ultrahigh-performance liquid chromatography combined with quadrupole-time of flight mass spectrometry,” Journal of Food Quality, vol. 2020, Article ID 8865725, 10 pages, 2020.
View at: Publisher Site | Google Scholar
Q. Q. Xu, B. H. Bao, L. Zhang et al., “UPLC-QTOF-MS analysis and multicomponent quantitative analysis of cayratia japonica,” Journal of Nanjing University Traditional Chinese Medicine, vol. 36, no. 4, pp. 517–524, 2020.
View at: Google Scholar
X. N. Lu, Y. W. Zheng, F. Wen et al., “Study of the active ingredients and mechanism of Sparganii rhizoma in gastric cancer based on HPLC-Q-TOF–MS/MS and network pharmacology,” Scientific Reports, vol. 11, no. 1, p. 1905, 2021.
View at: Publisher Site | Google Scholar
Y.-Y. Shi, S.-H. Guan, R.-N. Tang, S.-J. Tao, and D.-A. Guo, “Simultaneous determination of atractylenolide II and atractylenolide III by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of atractylodes macrocephala rhizoma extract,” Biomedical Chromatography, vol. 26, no. 11, pp. 1386–1392, 2012.
View at: Publisher Site | Google Scholar
X. X. Yan, H. Zhang, F. K. Zhang et al., “Determination of five nucleosides in Hericium erinaceus from different habitats by high performance liquid chromatography,” Chinese Journal of Traditional Medical Science and Technology, vol. 25, no. 4, pp. 520–522, 2018.
View at: Google Scholar
K. Mori, H. Kikuchi, Y. Obara et al., “Inhibitory effect of hericenone B from Hericium erinaceus on collagen-induced platelet aggregation,” Phytomedicine, vol. 17, no. 14, pp. 1082–1085, 2010.
View at: Publisher Site | Google Scholar
X. J. Ding, L. Xiong, Q. M. Zhou et al., “Advances in studies on chemical structure and pharmacological activities of natural nucleosides,” Journal of Chengdu University of TCM, vol. 41, no. 2, pp. 102–108, 2018.
View at: Google Scholar
J. H. Qi and F. X. Dong, “Research progress of the pharmacological action of flavonoids,” Journal of Beijing Union University, vol. 34, no. 3, pp. 89–92, 2020.
View at: Google Scholar

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Copyright © 2021 Fei Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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