Screening and Functional Verification of Selectable Marker Genes for Cordyceps militaris

Figure 4

Functional verification of the bar gene in C. militaris. (a) Conidia of C. militaris. (b) Colonies of C. militaris on the selective medium containing 300 μg/mL glufosinate ammonium and 300 μg/mL cefotaxime. (c) A C. militaris transformant (left) and a wild-type C. militaris (right) were inoculated on the PDA medium containing glufosinate ammonium (300 μg/mL) and cultured at 25°C for 18 days (including 12 days under dark conditions and 6 days under light conditions). (d) PCR amplification of the bar gene from a randomly selected C. militaris transformant. M:DNA marker; lane 1: 836 bp fragment of the bar gene expression cassette.

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