Isolation and Characterization of Gram-Negative Bacterial Species from Pasteurized Dairy Products: Potential Risk to Consumer Health

Table 1

PCR primer sequences and their targets used in this work.

Target gene	Primer sequence (5′⟶3′)	Product size (bp)	Reference (s)
Variable regions of 16S rRNA gene (V1–V9)
27F	AGA GTT TGA TCC TGG CTC AG	1500	Universal primers
1492R	GGT TAC CTT GTT ACG ACT T
Coliform/Salmonella
phoP	ATGCAAAGCCCGACCATGACG GTATCGACCACCACGATGGTT	299	13
Hin	CTAGTGCAAATT-GTGACCGCA CCCCATCGCGCTACTGGTATC	236	13
β-Lactamases
blaTEM	TCGCCGCATACACTATTCTCAGAATGAC	422	15
	CAGCAATAAACCAGCCAGCCGGAAG
blaCTX-M	ATGTGCAGYACCAGTAARGTKATGGC	590	15
	GGTRAARTARGTSACCAGAAYCAGCGG
blaSHV	TGTATTATCTC(C/T)CTGTTAGCC(A/G)CCCTG	739	15
	GTCCTGCTTTGTTATTCGGGCCAAGC