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Research Article | Open Access
Claudia Petrarca, Emanuela Clemente, Luca Di Giampaolo, Renato Mariani-Costantini, Kerstin Leopold, Roland Schindl, Lavinia V. Lotti, Rocco Mangifesta, Enrico Sabbioni, Qiao Niu, Giovanni Bernardini, Mario Di Gioacchino, "Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions", Journal of Immunology Research, vol. 2014, Article ID 295092, 8 pages, 2014. https://doi.org/10.1155/2014/295092
Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions
There is concern about the possible toxicity of palladium nanoparticles (Pd-NP), as they are released in the environment through many applications. We previously studied the toxicity of Pd-NP at high concentrations; here we address the possible toxicity of Pd-NP at low, subtoxic doses. In particular, we have exposed normal human PBMC entering into the first in vitro mitotic division to Pd-NP and to Pd(IV) ions to evaluate ROS generation and cell cycle progression. We have measured a statistically significant increase of intracellular ROS in Pd(IV) exposed cells, but not in Pd-NP exposed cells. TEM revealed accumulation of lipid droplets and autophagic and mitophagic vacuoles, which appeared more conspicuous in cells exposed to Pd(IV) ions than to Pd-NP. Pd-NP were visible in the cytoplasm of Pd-NP exposed cells. Pd-NP addition was associated with a significant increase of cells within the G0/G1-phase and a significant reduction in GS- and G2/M-phases. Cells exposed to Pd(IV) ions showed a significant amplification of these cell cycle alterations. These results suggest that ions, per se or released by NPs, are the true inducers of Pd toxicity. It will be essential to verify whether the observed disturbance represents a temporary response or might result in permanent alterations.
Palladium (Pd) is a component of the catalytic converters that promotes reduction and oxidation of pollutants in exhaust gases to less harmful ones . Catalytic converters emit Pd mainly as particulate matter in the breathable size range ( μm) [2, 3], containing also Pd-NP ( nm) . Indeed, after the introduction of catalytic converters based on the Pt group elements, the level of Pd (also in the form of nanoparticles) rapidly increased in the areas bordering roads [5–10]. At the same time, in Europe, allergic sensitization to Pd increased [11–14]. A causative role for Pd(II) ions in the development of allergic symptoms has been deduced from studies on occupationally exposed people [14–16] and people wearing Pd-containing dental alloys  and is sustained by laboratory data . Pd being chemically related to nickel, one of the most important sensitizing metals, phenomena of cross-reactivity might contribute to the increased frequency of immune reactions to Pd [19–21]. Few data are available to explain the role of Pd-NP for the allergic sensitization. Our recent data confirm the sensitizing power of Pd(II) and Pd(IV) ions [22, 23]. Moreover, we observed that Pd-NP can induce secretion of INF-γ, a Th1 cytokine involved in type IV immune reactions  suggesting a role for polluting Pd-NP in the raised frequency of allergic contact dermatitis to Pd in people living in urban settings .
On the other hand, no data are available for the possible involvement of Pd in cancer development, which is one of the major concerns about metal-based NPs. In the ionic form, Pd(II) does not induce significant genotoxicity , but no extrapolations are possible to Pd-NP. In fact, NPs have peculiar physical properties that may not be simply predicted by data from the corresponding ions. However, ions can be released from NPs and contribute to NP toxicity. Cobalt represents an example of these events. Recently, our group found that highly cytotoxic Co2+ ions are released from Co-NPs, but only nanoparticles are rapidly accumulated within the nucleus and interact with DNA , induce transformation , and affect molecular pathways implicated in carcinogenesis and inflammation . All these findings were obtained at rather high exposure concentrations. In the present work, we have studied the effects of low, subtoxic Pd-NP doses. As a target, we used cells of the immune system, taking into account that palladium can act as hapten able to induce an immune reaction. In particular, to study the possible interference of Pd-NP in the cell cycle progression, we have exposed to Pd-NP normal human PBMCs entering the first in vitro mitotic division.
2. Materials and Methods
2.1. Palladium Model Nanoparticles and Palladium Ions
Zerovalent Pd-NP (2–8 nm diameter) were produced and characterized as described previously . The Pd-NP stock solution did not contain Pd ions, even after long time from preparation, as assessed by low resolution inductively coupled plasma mass spectrometry. To exclude the presence of agglomerates, the Pd-NP stock solution was treated by ultrasonic bath for 10 min at 100 Hz (Elmasonic S, Elma, Singen, Germany) and then immediately diluted in complete culture medium at working concentrations and given to the cells straight away. Potassium hexachloropalladate (K2PdCl6, Sigma Aldrich, Milan, Italy) (Pd(IV) ion) was used at subtoxic concentration, for comparison.
Human peripheral blood mononuclear cells (PBMCs) from 3 healthy donors were isolated by density gradient centrifugation using a commercial separation medium consisting of a mixture of Ficoll 400 and sodium diatrizoate (Lymphoprep, StemCell Technologies, Voden Medical Instruments, Milan, Italy), according to standard manufacturer procedure. Cells were seeded at 500,000 cells mL−1 in RPMI-1640 medium supplemented with 10% fetal calf serum (complete culture medium) containing 5 μg mL−1 phytohemagglutinin-L (PHA-L) (Sigma Aldrich, Milan, Italy), an initiator of mitosis of normal human leukocytes. To evaluate the effect of the mitogen on cell viability and number, the PHA-stimulated cells were examined every 24 h for 5 days by trypan blue exclusion using a Neubauer counting chamber as described . After 48 hours of culture in the presence of PHA, Pd-NP (or Pd(IV) ions or an equal volume of the vehicle, as controls) were added to the cultures for further 48 hours and finally harvested appropriately for analysis. Experiments were done in triplicate.
2.3. Cytotoxicity Evaluation
Cytotoxicity was assessed by MTT (3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide) test according to the standard procedure. Briefly, PBMCs (40,000 per well in 200 μL culture medium, four replicates) were seeded in a 96-well culture plate, incubated at 37°C, 5% CO2, and exposed to increasing concentrations of Pd-NP or Pd(IV) ion (0.1, 1, 5, 10, 20, 40, 80 μg mL−1) or to an equal volume of vehicle (RPMI, 10% FCS) to assess 100% viability.
At the end of the exposure time, a washing step was performed to remove the incubation medium containing unreacted MTT and Pd-NP or Pd(IV) ion. Then, 20 μL of the MTT solution (5 mg mL−1) was added to each well and incubated for an additional 3 hours. The colored formazan crystals produced from MTT reduction were dissolved in 200 μL of dimethyl sulfoxide (DMSO). The optical density (O.D.) values of the solutions were measured at 540 nm as excitation wavelength using a spectrophotometer plate reader (Applied Biosystems, Life Technologies, Monza, Italy). Also, negative controls (i.e., complete culture medium alone or complete culture medium containing Pd-NP) have been tested and no interference with the colorimetric assay was observed, at all concentrations used. Cells without any treatment were assumed as positive control (100% viability).
The highest concentration that, after 48 h incubation, caused signal reduction (cell death) by less than 10% compared to the control was used for the experiments.
2.4. Detection of Intracellular Reactive Oxygen Species
The production of intracellular reactive oxygen species (ROS) was indirectly measured using dichlorodihydrofluorescein diacetate (DCFH-DA), a nonfluorescent and cell-permeant substance converted by endogenous esterases to dichlorofluorescein (DCFH), and nonfluorescent nonpermeant compound that cellular ROS oxidize proportionally to fluorescent dichlorofluorescein (DCF). Briefly, 200,000 PBMCs per well were seeded, in quadruplicate samples. After 48 hours, cells were washed with phosphate-buffered saline (PBS) and incubated with 10 μM DCFH-DA, for 30 min, at 37°C. Finally, the fluorescence emitted by the oxidized form of DCFH-DA was measured using a CytoFluor fluorescence multiwell plate reader (Applied Biosystems, Life Technologies, Monza, Italy) with the excitation fluorescence wavelength at 485 nm and the emission one at 530 nm.
2.5. Transmission Electron Microscopy
PBMCs were fixed overnight at 4°C in 2% paraformaldehyde and 2% glutaraldehyde in PBS, postfixed in 1% osmium tetroxide in veronal acetate buffer (pH 7.4) for 2 h at room temperature, stained with 2% uranyl acetate, dehydrated in acetone, and embedded in Epon 812. Thin sections were examined under a Philips CM10 TEM after poststaining with uranyl acetate and lead hydroxide.
2.6. Cell Cycle Assay
Cells were fixed in 70% cold ethanol, stained with 50 μg/mL propidium iodide in PBS buffer containing 200 μg/mL RNase (DNase-free, Sigma Aldrich, Milan, Italy), and analyzed using a FACSCalibur flow cytometer equipped with the CellQuest software (Becton Dickinson). Debris were excluded from the analysis after gating them out by setting the forward scatter versus side scatter plot on the viable cells area. Cell doublets and aggregates were excluded by gating FL2-area versus FL2-width. The low flow rate mode (400–500 events sec−1) was used to record 20,000 nondebris events for each sample. PI fluorescence data were collected using the linear amplification mode. DNA content was assessed by placing the G1 peak at channel 400. Finally, data were analysed using ModFit LT software (Verity Software House, Toshan, ME, USA).
2.7. Statistical Analysis
Statistical analysis was performed using GraphPad Prism 5.0 software (La Jolla, CA, USA). MTT and ROS data were analyzed by Student’s -test; cell cycle data were analyzed by three- and four-way analysis of variance (ANOVA) with Tukey multiple comparisons correction, when appropriate. The comparison between the different groups in different concentrations was performed with the -test. In all cases the threshold for significance was set at .
PBMCs, which are recognized as quiescent cells (G0 state), were induced to enter the cell cycle by in vitro stimulation with PHA, a well-known mitosis inducer. After a latency period of 48 hours, the number of cells started to increase and doubled within further 72 hours. Pd-NP were added 48 hours after PHA, at the beginning of the growth phase. After 4 hours of exposure to Pd-NP, no evident changes in cell viability were observed at any tested dose. Prolongation of the exposure time to 24 hours showed a concentration-dependent decrease of viability up to 40 μg mL−1 and death at 80 μg mL−1. At 48 hours, also 40 μg mL−1 treated cells were incapable of metabolic conversion of MTT, and the same result was obtained after 72 hours of exposure. The viability data for Pd-NP are shown in Figure 1. Pd(IV) ions were more toxic, causing almost complete loss of cell viability earlier, at 48 hours and at lower dose (5 μg mL−1). Therefore, the following experiments were performed using the nontoxic doses of 10 μg mL−1 Pd-NP and 0.1 μg mL−1 Pd(IV) ions, for 48 hours.
A moderate, but not statistically significant increase of intracellular ROS was measured both in controls and in Pd-NP exposed cells, whereas ROS significantly () increased after Pd(IV) exposure by more than 30% of the control (Figure 2). Electron microscopy revealed that, as compared to controls, PBMCs exposed to Pd-NP and to Pd ions shared marked subcellular alterations (Figure 3). Most notably, these included the presence of numerous autophagosomal vacuoles containing damaged mitochondria and/or undigested cytoplasmic material, as well as the accumulation of multilamellar bodies and lipid droplets. Such alterations were more marked in PBMCs exposed to Pd ions, being already well evident at our nontoxic concentration. With both treatments, mitochondria showed evidence of damage, as indicated by condensation and loss or swelling of cristae. Furthermore, in both cases, there was evidence of increased plasma membrane ruffling and nuclei were more indented and/or convoluted compared to controls. In PBMCs exposed to Pd-NP, electron-dense agglomerates of nanosized particulate material within or adjacent to cytoplasmic vesicles were consistent with the internalization of Pd-NP.
The examination of cell cycle (Figure 4) confirmed the quiescent/resting state of all unstimulated PBMCs either freshly isolated (w/o PHA, 0 hours) or cultured for the entire experimental period (w/o PHA, 96 hours), as they were found in the G0 state. The addition of the polyclonal activator PHA stimulated cell division, as shown by their distribution throughout all phases of the cell cycle (G0/G1: %; S: %; G2/M: %). The exposure of PHA-activated PBMCs to 10 μg mL−1 Pd-NP for the time allowing the completion of at least one cell division (48 h) was associated with a significant reduction of cells synthesizing DNA (S-phase) (% versus %, ), a significant increase of cells within the G0/G1-phase (% versus %, ), and a significant reduction of cells in G2/M-phase (% versus %, ), compared with unexposed control cells. A different cell cycle outline was observed for Pd(IV) ion exposed cells, with significantly higher G0/G1 accumulation compared to Pd-NP exposed cells (% versus %, ), lower percent of cells in S-phase (% versus %, ), and no cells engaged in the cell division (G2/M). The vehicles alone did not cause significant changes of the cell cycle distribution. Also, PBMCs were evaluated for cell cycle distribution after exposure to Pd-NP and Pd(IV), in absence of PHA stimulation; in no case were these compounds found to induce cell cycle initiation and all cells remained in the G0 phase (data not shown).
Sub-G0/G1 cells, indicative of cell death, were largely absent from the analyzed populations, for all tested samples. All cell cycle data are summarized in (Table 1).
|Data from three experiments are shown as mean ± S.D. *P < 0.05 Pd-NP and Pd(IV) vs not exposed.|
In the present work, we have found that Pd-NP exposure is associated with an increased percentage of mitogen-activated PBMCs with a diploid DNA content, indicative of maintenance of G0 state or prolongation/arrest in G1-phase. Studying the influence of potential toxicants on the G1-phase of the cell cycle is of central importance; in fact, most, if not all, human cancer types show a deregulated control of G1 progression, a period in which cells decide whether to start proliferation or stay quiescent [31, 32]. Important processes, such as increase in cell size and centrosome duplication, are initiated in G1 and are thus controlled. Control is conducted by checkpoint complexes that stall the cell cycle until the appropriate repair is completed. If such repair is not achieved, the cell may pause or undergo senescence or apoptosis. Alteration of repair (controlled by caretakers) and checkpoint complexes (controlled by gatekeepers genes) is central in oncogenesis .
In our experiments, PBMCs, which are mostly quiescent cells in the G0 state and prompted to enter the cell cycle by in vitro stimulation with PHA, were exposed to low noncytotoxic dose-time combinations of Pd-NP and Pd ions. Under these experimental conditions, Pd-NP gain entry to the cytosol, where they appear in clusters lacking a membranous envelope, suggesting the possibility to exert a catalysing redox activity  on cellular elements.
At the ultrastructural level the alterations observed in the PBMCs exposed to Pd-NP largely corresponded to those observed in the PBMCs exposed to Pd ions. Most notably, in both cases the cells showed evidence of mitochondrial damage and of mitophagy, that is, autophagic elimination of damaged mitochondria. Mitophagy is a key protective mechanism against mitochondrial damage and the consequent ROS-induced cellular alterations . The accumulation of abundant multilamellar bodies is also in agreement with the macroautophagic response, as these structures originate via autophagy and reflect the accumulation of membrane lamellar material selectively resistant to lysosomal degradation within autophagolysosomes. Lipid droplets, specialized organelles for the deposition and storage of neutral lipids, are associated with common pathologies linked to lipid accumulation and mitochondrial damage. Their accumulation in PBMCs exposed to Pd is fully consistent with the loss of mitochondrial fatty acid beta oxidation due to mitochondrial damage, that, in turn, results in lipid accumulation and loss of ATP production.
In conclusion, the morphological evidence strongly suggests that Pd ions, per se or dissolved from NPs, accumulate in the mitochondria, as we recently demonstrated in the case of cobalt . Here, acting as catalyst, ionic Pd may interfere with the oxidative phosphorylation (OXPHOS). The mitochondria, whose respiratory activity is damaged by the influx of Pd ions, will be poor ATP producers and will release excessive amounts of ROS. The ROS-induced cellular stress is predicted to arrest the cell cycle, acting via oxidative stress-sensitive cell cycle regulator genes such as P53 and CDKN2a, which we have recently found to be implicated in the response to Co particles in Balb/3T3 cells . In turn, ROS and ATP depletion activate cell death pathways, which induce the release of proapoptotic proteins from mitochondria. Macroautophagy may mitigate these effects by the selective sequestration and subsequent degradation of the dysfunctional mitochondria and other ROS-damaged subcellular structures before the activation of prodeath pathways [30, 35, 36]. Thus, macroautophagy, and, in particular, mitophagy, most likely represents a key prosurvival pathway in cells exposed to palladium.
Autophagy has been demonstrated to be marker of heavy metals toxicity for human hematopoietic progenitor cells , representing a protecting and recycling catabolic pathway removing damaged organelles and molecules . The cellular outcome of inducing autophagy in response to stress is complex and depends on the cellular context, type, and magnitude of stress. In mild stress conditions, autophagy may enhance cell survival by allowing the cell to engage DNA repair mechanisms and checkpoint activation . Nontoxic dose of Pd ions induced a more marked production of ROS relative to Pd-NP, contrary to almost all other metal-based NPs . However, local ROS generation at sites of Pd-NP accumulation, not measurable by the overall method used, might be possible at the nontoxic doses applied in our experiments. In fact, local concentration is important for the cellular function of ROS . On the other hand, changes in ROS generation may modulate autophagic processes, as ROS can modify activating molecules to stimulate or inhibit autophagy and engages in cross-talk with autophagy in both cell signaling and protein damage .
Mechanisms of cell damage by Pd-NP can be also explained through cell cycle disturbance by the withdraw/inactivation of nutrients and/or PHA or by modulation/inhibition of downstream signaling due to their catalytic activity, as described for polystyrene-coated Pd nanoparticles . Therefore, Pd-NP might produce an effect similar to nutrient depletion in inducing autophagy in the G1 phase . It is possible that Pd-NP interference with mitogen/growth factors would prolong the G1-phase checkpoint called restriction point that prevents cells from entering the cycle until they have accumulated a certain threshold of mitogen-induced events .
The described findings suggest a potentially harmful role for low dose Pd ions and Pd-NP in hematopoietic cells leaving quiescence. However, it will be essential to verify whether the observed disturbance of the transition from quiescence to cell division represents a temporary response or might lead to permanent alterations of the cell functions. To this aim, long-term exposure to low dose of Pd (ions and NPs) and single cell-based technologies are envisaged.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
- J. Abthoff, W. Zahn, G. Loose, and A. Hirschmann, “Serial use of palladium for three-way-catalysts with high performance,” Motortechnische Zeitschrift, vol. 55, no. 5, pp. 292–297, 1994.
- M. Moldovan, M. M. Gómez, and M. A. Palacios, “Determination of platinum, rhodium and palladium in car exhaust fumes,” Journal of Analytical Atomic Spectrometry, vol. 14, no. 8, pp. 1163–1169, 1999.
- H. M. Prichard and P. C. Fisher, “Identification of platinum and palladium particles emitted from vehicles and dispersed into the surface environment,” Environmental Science and Technology, vol. 46, no. 6, pp. 3149–3154, 2012.
- J. D. Whiteley and F. Murray, “Anthropogenic platinum group element (Pt, Pd and Rh) concentrations in road dusts and roadside soils from Perth, Western Australia,” Science of the Total Environment, vol. 317, no. 1–3, pp. 121–135, 2003.
- F. Zereini, C. Wiseman, and W. Püttmann, “Changes in palladium, platinum, and rhodium concentrations, and their spatial distribution in soils along a major highway in Germany from 1994 to 2004,” Environmental Science and Technology, vol. 41, no. 2, pp. 451–456, 2007.
- K. Leopold, M. Maier, S. Weber, and M. Schuster, “Long-term study of palladium in road tunnel dust and sewage sludge ash,” Environmental Pollution, vol. 156, no. 2, pp. 341–347, 2008.
- J. Schäfer, D. Hannker, J. D. Eckhardt, and D. Stüben, “Uptake of traffic-related heavy metals and platinum group elements (PGE) by plants,” Science of the Total Environment, vol. 215, no. 1-2, pp. 59–67, 1998.
- B. Sures, S. Zimmermann, J. Messerschmidt, and A. von Bohlen, “Relevance and analysis of traffic related platinum group metals (Pt, Pd, Rh) in the aquatic biosphere, with emphasis on palladium,” Ecotoxicology, vol. 11, no. 5, pp. 385–392, 2002.
- S. Zimmermann, J. Messerschmidt, A. Von Bohlen, and B. Sures, “Uptake and bioaccumulation of platinum group metals (Pd, Pt, Rh) from automobile catalytic converter materials by the zebra mussel (Dreissena polymorpha),” Environmental Research, vol. 98, no. 2, pp. 203–209, 2005.
- J. Brasch and J. Geier, “Patch test results in schoolchildren. Results from the Information Network of Departments of Dermatology (IVDK) and the German Contact Dermatitis Research Group (DKG),” Contact Dermatitis, vol. 37, no. 6, pp. 286–293, 1997.
- L. F. Filon, D. Uderzo, and E. Bagnato, “Sensitization to palladium chloride: a 10-year evaluation,” American Journal of Contact Dermatitis, vol. 14, no. 2, pp. 78–81, 2003.
- S. Wöhrl, W. Hemmer, M. Focke, M. Götz, and R. Jarisch, “Patch testing in children, adults, and the elderly: influence of age and sex on sensitization patterns,” Pediatric Dermatology, vol. 20, no. 2, pp. 119–123, 2003.
- B. Santucci, C. Valenzano, M. de Rocco, and A. Cristaudo, “Platinum in the environment: frequency of reactions to platinum-group elements in patients with dermatitis and urticaria,” Contact Dermatitis, vol. 43, no. 6, pp. 333–338, 2000.
- P. J. Linnett and E. G. Hughes, “20 Years of medical surveillance on exposure to allergenic and non-allergenic platinum compounds: the importance of chemical speciation,” Occupational and Environmental Medicine, vol. 56, no. 3, pp. 191–196, 1999.
- R. Merget and G. Rosner, “Evaluation of the health risk of platinum group metals emitted from automotive catalytic converters,” Science of the Total Environment, vol. 270, no. 1–3, pp. 165–173, 2001.
- A. Cristaudo, V. Bordignon, F. Petrucci et al., “Release of palladium from biomechanical prostheses in body fluids can induce or support PD-specific IFNγ T cell responses and the clinical setting of a palladium hypersensitivity,” International Journal of Immunopathology and Pharmacology, vol. 22, no. 3, pp. 605–614, 2009.
- I. Iavicoli, G. Carelli, A. Marinaccio et al., “The effects of sub-acute exposure to palladium on cytokines in male Wistar rats,” International Journal of Immunopathology and Pharmacology, vol. 19, no. 4, pp. 21–24, 2006.
- J. T. Minang, I. Areström, M. Troye-Blomberg, L. Lundeberg, and N. Ahlborg, “Nickel, cobalt, chromium, palladium and gold induce a mixed Th1- and Th2-type cytokine response in vitro in subjects with contact allergy to the respective metals,” Clinical and Experimental Immunology, vol. 146, no. 3, pp. 417–426, 2006.
- J. Muris, C. J. Kleverlaan, A. J. Feilzer, and E. Valentine-Thon, “Reactivity to sodium tetrachloropalladate (Na2[PdCl 4]) compared to PdCl2 and NiCl2 in lymphocyte proliferation tests,” Allergy, vol. 64, no. 8, pp. 1152–1156, 2009.
- J. Kielhorn, C. Melber, D. Keller, and I. Mangelsdorf, “Palladium—a review of exposure and effects to human health,” International Journal of Hygiene and Environmental Health, vol. 205, no. 6, pp. 417–432, 2002.
- P. Boscolo, L. Di Giampaolo, M. Reale et al., “Different effects of platinum, palladium, and rhodium salts on lymphocyte proliferation and cytokine release,” Annals of Clinical and Laboratory Science, vol. 34, no. 3, pp. 299–306, 2004.
- M. Di Gioacchino, N. Verna, L. Di Giampaolo et al., “Immunotoxicity and sensitizing capacity of metal compounds depend on speciation,” International Journal of Immunopathology and Pharmacology, vol. 20, no. 2, pp. 15–22, 2007.
- P. Boscolo, V. Bellante, K. Leopold et al., “Effects of palladium nanoparticles on the cytokine release from peripheral blood mononuclear cells of non-atopic women,” Journal of Biological Regulators and Homeostatic Agents, vol. 24, no. 2, pp. 207–214, 2010.
- L. Migliore, G. Frenzilli, C. Nesti, S. Fortaner, and E. Sabbioni, “Cytogenetic and oxidative damage induced in human lymphocytes by platinum, rhodium and palladium compounds,” Mutagenesis, vol. 17, no. 5, pp. 411–417, 2002.
- E. Sabbioni, S. Fortaner, M. Farina et al., “Cytotoxicity and morphological transforming potential of cobalt nanoparticles, microparticles and ions in Balb /3T3 mouse fibroblasts: an in vitro model,” Nanotoxicology, vol. 8, no. 4, pp. 455–464, 2014.
- E. Sabbioni, S. Fortaner, and M. Farina, “Interaction with culture medium components, cellular uptake and intracellular distribution of cobalt nanoparticles, microparticles and ions in Balb/3T3 mouse fibroblasts,” Nanotoxicology, vol. 8, pp. 455–464, 2014.
- S. Perconti, G. M. Aceto, F. Verginelli, F. Napolitano, C. Petrarca, and G. Bernardini, “Distinctive gene expression profiles in Balb /3T3 cells exposed to low dose cobalt nanoparticles, microparticles and ions: potential nanotoxicological relevance,” Journal of Biological Regulators & Homeostatic Agents, vol. 27, pp. 443–454, 2013.
- K. Leopold, M. Maier, and M. Schuster, “Preparation and characterization of Pd/Al2O3 and Pd nanoparticles as standardized test material for chemical and biochemical studies of traffic related emissions,” Science of the Total Environment, vol. 394, no. 1, pp. 177–182, 2008.
- M. di Gioacchino, C. Petrarca, A. Perrone et al., “Autophagy as an ultrastructural marker of heavy metal toxicity in human cord blood hematopoietic stem cells,” Science of the Total Environment, vol. 392, no. 1, pp. 50–58, 2008.
- M. Malumbres and M. Barbacid, “Cell cycle, CDKs and cancer: a changing paradigm,” Nature Reviews Cancer, vol. 9, no. 3, pp. 153–166, 2009.
- M. Malumbres and A. Carnero, “Cell cycle deregulation: a common motif in cancer,” Progress in cell Cycle Research, vol. 5, pp. 5–18, 2003.
- B. Delaval and D. Birnbaum, “A cell cycle hypothesis of cooperative oncogenesis (Review),” International Journal of Oncology, vol. 30, no. 5, pp. 1051–1058, 2007.
- R. M. Yusop, A. Unciti-Broceta, E. M. V. Johansson, R. M. Sánchez-Martín, and M. Bradley, “Palladium-mediated intracellular chemistry,” Nature Chemistry, vol. 3, no. 3, pp. 239–243, 2011.
- D. A. Kubli and Å. B. Gustafsson, “Mitochondria and mitophagy: the yin and yang of cell death control,” Circulation Research, vol. 111, no. 9, pp. 1208–1221, 2012.
- M. Di Gioacchino, C. Petrarca, A. Perrone et al., “Autophagy in hematopoietic stem/progenitor cells exposed to heavy metals: biological implications and toxicological relevance,” Autophagy, vol. 4, no. 4, pp. 537–539, 2008.
- F. Reggiori, M. Komatsu, K. Finley, and A. Simonsen, “Autophagy: more than a nonselective pathway,” International Journal of Cell Biology, vol. 2012, Article ID 219625, 18 pages, 2012.
- O. Surova and B. Zhivotovsky, “Various modes of cell death induced by DNA damage,” Oncogene, vol. 32, no. 33, pp. 3789–3797, 2013.
- Y. Yang, C. Zhang, and Z. Hu, “Impact of metallic and metal oxide nanoparticles on wastewater treatment and anaerobic digestion,” Environmental Sciences, vol. 15, no. 1, pp. 39–48, 2013.
- G. Liou and P. Storz, “Reactive oxygen species in cancer,” Free Radical Research, vol. 44, no. 5, pp. 479–496, 2010.
- J. Lee, S. Giordano, and J. Zhang, “Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling,” Biochemical Journal, vol. 441, no. 2, pp. 523–540, 2012.
- E. Tasdemir, M. C. Maiuri, N. Tajeddine et al., “Cell cycle-dependent induction of autophagy, mitophagy and reticulophagy,” Cell Cycle, vol. 6, no. 18, pp. 2263–2267, 2007.
- D. Donjerkovic and D. W. Scott, “Regulation of the G1 phase of the mammalian cell cycle,” Cell Research, vol. 10, no. 1, pp. 1–16, 2000.
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