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BioMed Research International
Volume 2014 (2014), Article ID 182846, 7 pages
http://dx.doi.org/10.1155/2014/182846
Research Article

Characterization of Imidazoline Receptors in Blood Vessels for the Development of Antihypertensive Agents

1Department of Food Science, National Pingtung University of Science and Technology, Neipu, Pingtung 91201, Taiwan
2College of Medicine and Life Science, Chung Hwa University of Medical Technology, Rende District, Tainan City 71703, Taiwan
3Department of Radiation Oncology, Taipei Medical University-Shuang Ho Hospital, and College of Medicine, Taipei Medical University, Taipei City 10361, Taiwan
4Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City 70101, Taiwan
5Department of Obs/Gyn, Tainan Sin-Lau Hospital, The Presbyterian Church in Taiwan, Tainan City 70142, Taiwan
6Department of Chinese Medicine, Tainan Sin-Lau Hospital, The Presbyterian Church in Taiwan, Tainan City 70142, Taiwan

Received 16 February 2014; Accepted 9 March 2014; Published 3 April 2014

Academic Editor: Juei-Tang Cheng

Copyright © 2014 Mei-Fen Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

It has been indicated that activation of peripheral imidazoline I2-receptor (I-2R) may reduce the blood pressure in spontaneously hypertensive rats (SHRs). Also, guanidinium derivatives show the ability to activate imidazoline receptors. Thus, it is of special interest to characterize the I-2R using guanidinium derivatives in blood vessels for development of antihypertensive agent(s). Six guanidinium derivatives including agmatine, amiloride, aminoguanidine, allantoin, canavanine, and metformin were applied in this study. Western blot analysis was used for detecting the expression of imidazoline receptor in tissues of Wistar rats. The isometric tension of aortic rings isolated from male rats was also estimated. The expression of imidazoline receptor on rat aorta was identified. However, guanidinium derivatives for detection of aortic relaxation were not observed except agmatine and amiloride which induced a marked relaxation in isolated aortic rings precontracted with phenylephrine or KCl. Both relaxations induced by agmatine and amiloride were attenuated by glibenclamide at concentration enough to block ATP-sensitive potassium () channels. Meanwhile, only agmatine-induced relaxation was abolished by BU224, a selective antagonist of imidazoline I2-receptors. Taken together, we suggest that agmatine can induce vascular relaxation through activation of peripheral imidazoline I2-receptor to open channels. Thus, agmatine-like compound has the potential to develop as a new therapeutic agent for hypertension in the future.

1. Introduction

Hypertension is known as the main risk parameters in patients with cardiovascular diseases, such as myocardial infarction and stroke. Many agents used in clinics are mentioned to produce side effects. Thus, development of the better agent to handle hypertension is urgent [1].

Imidazoline receptors are introduced to play a role in cardiovascular regulation [2, 3]. In recent, 3 subtypes of imidazoline receptors have been proposed; activation of I-1 receptors regulates the blood pressure through central nervous system [4], whereas I-3 receptors participate in insulin release [5] and activation of I-2 receptors (I-2R) increases glucose uptake into muscle cells [6, 7]. The clinical used antihypertensive agent rilmenidine may reduce blood pressure via an activation of imidazoline I1-receptors in brain to lower sympathetic tone [8, 9]. But, application of rilmenidine in hypertension is usually to produce some side effects such as mental depression, insomnia, and drowsiness. Thus, development of new agent for management of hypertension is essential. Recently, an activation of peripheral imidazoline I2-receptor (I-2R) was documented to produce antihypertensive actions in spontaneous hypertensive rats (SHRs) [10]. Thus, peripheral I-2R seems a potential target in development of antihypertensive drugs without side effects of sympathetic inhibition.

It has been documented that compounds with guanidine-like structures may bind to imidazoline receptors [11]. Thus, it is of special interest to investigate the effect of guanidinium derivatives on peripheral I-2R for vasodilatation. Then, this may help the development of new agent(s) for hypertension in the future.

2. Material and Methods

2.1. Animals

The male Wistar rats, weighing from 250 to 300 g, were obtained from the Animal Center of National Cheng Kung University Medical College. Animals were housed individually in plastic cages under standard laboratory conditions. We kept them under a 12 h light/dark cycle and had free access to food and water. All experiments were performed under anesthesia with 2% isoflurane to minimize the animals’ suffering. The animal experiments were approved and conducted in accordance with local institutional guidelines for the care and use of laboratory animals, and the experiments conformed to the Guide for the Care and Use of Laboratory Animals as well as the guidelines of the Animal Welfare Act.

2.2. Preparation of Isolated Aortic Rings

Isolation of aortas was performed as described previously [10] from Wistar rats. After sacrifice under anesthesia with pentobarbital (50 mg/kg), the thoracic aortas were removed to put in the oxygenated Krebs’ buffer (95% O2, 5% CO2). Aortas were cut into ring segments about 3 mm without fat and connective tissue. Then, as described previously [10], they were mounted in the organ baths containing 10 mL oxygenated Krebs’ buffer (95% O2, 5% CO2) at 37°C.

Similar to previous report [10], each ring was connected to strain gauges (FT03; Grass Instrument, Quincy, MA, USA) to measure the isometric tension through chart software (MLS023, Powerlab; AD Instruments, Bella Vista, NSW, Australia). Samples were mounted to stabilize for 2 h. Each ring was then stretched gradually for optimal resting tension at 1 g.

2.3. Vasodilatation Caused by Guanidinium Derivatives

After the stabilization of resting tone, a solution of either phenylephrine (Sigma-Aldrich, St. Louis, MO, USA) or KCl prepared in distilled water was added to the bathing buffer to induce a marked raise in vascular tone followed by a stable vasoconstriction (tonic contraction). The final concentration in the organ bath of both phenylephrine and KCl was 1 μmol/L and 50 mmol/L, similar to previous report [10]. Rings of the treated group were exposed to agmatine, amiloride, metformin, allantoin, canavanine, and aminoguanidine (10 μM) for recording the alterations in tonic contraction (vasodilatation). Relaxation is expressed as the decreased percentage in maximal tonic contraction.

2.4. Effects of Blockers on Guanidinium Derivatives-Induced Vasodilatation

Aortic rings were exposed to BU224 (Research Biochemical, Wayland, MA, USA), a selective antagonist of imidazoline I2-receptors, for 15 min prior to the addition of guanidinium derivatives into the organ bath. Glibenclamide (Tocris Cookson, Bristol, UK), as blocker specific for KATP channels, was administered in the same manner. The changes of vasodilatation after treatment with inhibitor were compared to vehicle-treated groups.

2.5. Western Blotting Analysis

Western blotting analysis was performed as the previous method [10] and we extracted protein from tissue homogenates using ice-cold radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase and protease inhibitors (50 mmol/L sodium vanadate, 0.5 mM phenylmethylsulphonyl fluoride, 2 mg/mL aprotinin, and 0.5 mg/mL leupeptin). Concentrations of protein were determined with a Bio-Rad protein assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Total proteins (30 μg) and were separated by SDS/polyacrylamide gel electrophoresis (10% acrylamide gel) using a Bio-Rad Mini-Protein II system. The protein was transferred to expanded polyvinylidene difluoride membranes (Pierce, Rockford, IL, USA) with a Bio-Rad Trans-Blot system. After transfer, the membranes were washed with PBS and blocked for 1 h at room temperature with 5% (w/v) skimmed milk powder in PBS. The manufacturer’s instructions were followed for the primary antibody reactions. Following blocking, the blots were developed using antibodies for imidazoline receptors (IR) (Abcam, Cambridge, UK). The blots were subsequently hybridized using horseradish peroxidase-conjugated goat anti-goat IgG (Jackson ImmunoResearch Laboratories, Inc., PA, USA) and developed using the Western Lightning Chemiluminescence Reagent PLUS (PerkinElmer Life Sciences Inc., Boston, MA, USA). Densities of the obtained immunosblots at 37 KDa for imidazoline receptors (IR) and 43 KDa for actin were quantified using Gel-Pro analyser software 4.0 (Media Cybernetics, Silver Spring, MD, USA).

2.6. Statistical Analysis

Results were expressed as mean ± SE of each group. Statistical analysis was carried out using Student’s -test analysis. Statistical significance was set as .

3. Results

3.1. Identification of Imidazoline Receptor Expression in Tissues Using Western Blotting Analysis

The anti-NISCH (imidazoline) antibody positively reacted with the tissue lysate prepared from heart, aorta, pancreas, skeletal muscle, kidney, prostate, and urinary bladder using western blotting analysis (Figure 1). The expression of imidazoline receptor in aorta can thus be identified.

182846.fig.001
Figure 1: Detection of the expressions of imidazoline receptors in tissue homogenates by western blot analysis. The anti-NISCH (imidazoline receptors) antibody positively reacted with tissue lysate of heart, liver, aorta, skeletal muscle (SM), kidney, prostate, and bladder by western blot analysis. All values are presented as mean ± SEM ().
3.2. Effects of Guanidinium Derivatives on Vascular Tone

Six guanidinium derivatives of agmatine, amiloride, metformin, allantoin, canavanine, and aminoguanidine were tested in current study. Aortic ring strips are markedly contracted by the application of phenylephrine (1 μmol/L) or KCl (50 mmol/L) as described previously [12]. Similar to the previous report [13], most guanidinium derivatives did not modify the vascular tone of aortic rings at the pharmacological concentration (10 μmol/L) but agmatine and amiloride significantly relaxed the tonic contraction of rats’ aortic rings induced by phenylephrine. Similarly, KCl-induced tonic vasoconstriction was relaxed by agmatine and amiloride (Figure 2).

fig2
Figure 2: Screening of guanidinium derivatives for vasodilatation. Guanidinium derivatives (10 μmol/L), agmatine, amiloride, metformin, allantoin, canavanine, and aminoguanidine induced relaxation in isolated aortic rings precontracted with 1 μmol/L phenylephrine (a) or 50 mmol/L KCl (b). Data represent the mean ± SEM of eight animals in each column. All values are presented as mean ± SEM (). * as compared to the precontracted value.
3.3. Effects of Imidazoline Receptor Antagonism on Agmatine or Amiloride-Induced Vasodilatation

Agmatine or amiloride at concentration of 10 μmol/L attenuated the tonic contraction of aortic rings induced by phenylephrine similar to that observed in KCl-induced tonic vasoconstriction. BU224 (0.01–1 μmol/L) produced a concentration-dependent inhibition of agmatine-induced relaxation in phenylephrine- or KCl-precontracted aortic rings (Figure 3(a)). However, BU224 did not modify the vascular relaxing action of amiloride (Figure 3(b)).

fig3
Figure 3: Effects of imidazoline receptor blockade on agmatine- or amiloride-induced vasodilatation. Effect of BU224 on concentration-dependent inhibition of agmatine- and amiloride- (10 μmol/L) induced relaxation in isolated aortic rings precontracted with 1 μmol/L phenylephrine ((a) and (b)) or 50 mmol/L KCl ((c) and (d)). All values are presented as mean ± SEM (). * and ** as compared to agmatine-treated group.
3.4. The Role of ATP-Sensitive K+ (KATP) Channels in Agmatine- or Amiloride-Induced Vasodilatation

Glibenclamide (0.1–10 nmol/L) produced a concentration-dependent inhibition of agmatine-induced vasodilatation in phenylephrine- or KCl-precontracted aortic rings (Figure 4(a)). Similar inhibitions were also observed in amiloride-induced vasodilatation (Figure 4(b)). However, as shown in these figures, glibenclamide at 10 nmol/L abolished amiloride-induced vasodilatation totally but not agmatine-induced vasodilatation.

fig4
Figure 4: Effects of KATP blockade on agmatine- or amiloride-induced vasodilatation. Inhibitory effect of glibenclamide on the agmatine- or amiloride- (10 μmol/L) induced relaxation in isolated aortic rings precontracted with 1 μmol/L phenylephrine ((a) and (b)) or 50 mmol/L KCl (c and d). All values are presented as mean ± SEM (). *, **, and *** as compared to the precontracted value.
3.5. Enhanced Vasodilatation in Aortic Rings by Cotreatment with Agmatine and Amiloride

At the maximal concentration (10 μmol/L), agmatine or amiloride relaxed the tonic contraction of aortic rings induced by phenylephrine in a way similar to that in KCl-induced tonic vasoconstriction. The vasodilatation in aortic ring was enhanced by cotreatment with agmatine and amiloride (Figure 5).

fig5
Figure 5: Vasodilatation in isolated aortic ring after cotreatment with agmatine and amiloride. Vasodilatation was enhanced by cotreatment with agmatine (10 μmol/L) and amiloride (10 μmol/L) in isolated aortic rings precontracted with 1 μmol/L phenylephrine (a) or 50 mmol/L KCl (b). All values are shown as mean ± SEM (). ** and *** as compared to the precontracted value.

4. Discussion

In the present study, we identified the expression of imidazoline receptor in aortic tissues isolated from rats. This is consistent with a previous report [10]. Then, we investigated the vasodilatation of guanidinium derivatives using agmatine, amiloride, metformin, allantoin, canavanine, and aminoguanidine. But only two agents, agmatine and amiloride, were effective for further characterizations. BU224, the well-known antagonist of imidazoline I2-receptors, showed no effect on amiloride-induced vascular relaxation at the dose effective to block agmatine-induced relaxation. Both agmatine- and amiloride-induced relaxations were attenuated by glibenclamide at concentration sufficient to block ATP-sensitive potassium (KATP) channels. However, glibenclamide with the concentration effective to abolish amiloride-induced vasodilatation failed to block agmatine-induced vasodilatation totally (Figure 4). Also, enhanced vasodilatation was observed in aortic ring receiving the cotreatment with agmatine and amiloride at maximal concentration. Thus, agmatine-induced vascular relaxation seems not through the same mechanism as amiloride, while agmatine is known as the ligand of imidazoline receptors.

The imidazoline receptors are known to involve in cardiovascular regulations [14, 15]. Vascular tone is introduced as the main parameter in blood pressure regulations [16]. Actually, blood pressure is regulated by complicated factors; changes of blood pressure are widely used as the total peripheral resistance which is primarily a function of the resistance terminal arterioles [17]. Compounds with guanidine-like structures are known to bind with imidazoline receptors [11]. In this study, we employed six guanidinium derivatives including agmatine, amiloride, metformin, allantoin, canavanine, and aminoguanidine to screen the effects on vascular tone under the pharmacological dosage of 10 μM. The results show that only agmatine and amiloride significantly relaxed the phenylephrine- or KCl-induced tonic contraction of aortic rings isolated from rats to consist with the previous reports [10, 18].

It has been mentioned that metformin can inhibit phenylephrine-mediated aortic contraction at the dosage of 2 mM [19]. It seems different with our results. Actually, the dosage used at 2 mM seems too high and the result is mostly considered as nonspecific action. Moreover, allantoin failed to produce vasodilatation in isolated aortic ring. This finding is further supporting our previous report showing that antihypertensive effect of allantoin is mainly through an activation of imidazoline receptor in central nervous system [20]. However, before now, no report mentioned the vasodilatation of canavanine or aminoguanidine. It is possible that the guanidine derivatives including canavanine and aminoguanidine may induce vasodilatation at the dose higher than that used in current study and this view needs more investigation in the future.

In an attempt to know the role of imidazoline I-2 receptor (I2-R) in agmatine- or amiloride-induced vasodilatations, imidazoline I-2R specific antagonist named BU224 was applied. Actually, the relaxation of agmatine was markedly reduced by pretreatment with BU224 at a concentration sufficient to block imidazoline I-2Rs. Thus, a direct effect of agmatine on I-2Rs can be identified. However, BU224 failed to modify the amiloride-induced vascular relaxation. The vessel dilatation of amiloride seems not to be through the activation of I-2Rs.

Due to a guanidino structure, amiloride binds to imidazoline through a high affinity for blockade of   at 0.1 μM and inhibition of at a higher concentration of 2 μM [21]. Thus, amiloride is widely used to distinguish the subtype of imidazoline I-2Rs. Previous study has also indicated that amiloride can induce vascular relaxation through an activation of Na+-H+ exchanger and consequently affect the KATP channel activity [22, 23]. In this study, BU224 failed to modify the 10 μM amiloride-induced vascular relaxation. It is possible that vasodilatation induced by amiloride at the concentration of 10 μM is mainly through the activation of KATP channels.

Potassium channels are mentioned as important in vascular relaxation [24]. ATP-sensitive potassium (KATP) channels are known to have four inwardly rectifying K+ channel subunits and four regulatory sulfonylurea receptors [25]. Many contractions-induced endogenous substances are related to inhibition of KATP channels [25, 26]. Activation of KATP channels may produce hyperpolarization to relax vascular tone consequently. KATP channels dysfunction in aortic cells has been introduce to the impaired vasodilatation and/or hypertension observed in deoxycorticosterone acetate (DOCA) salt hypertensive rats [27]. In the present study, the relaxation induced by agmatine or amiloride in rat aortic rings was abolished by pretreatment with glibenclamide at a concentration sufficient to block KATP channels, as described previously [10, 23, 28, 29]. Thus, there is no doubt that KATP channels are involved in the aortic relaxation induced by agmatine or amiloride. However, agmatine-induced aortic relaxation seems not so simple although KATP channel is responsible for vasodilatation in PE- or KCl-induced contractions.

Agmatine has been introduced as an endogenous ligand of imidazoline receptors [30]. Activation of imidazoline I-2R by agmatine has also been mentioned in adrenal gland [31]. In this study, we demonstrated that BU224 at the dose enough to block imidazoline I-2Rs inhibited agmatine-induced vasodilatation. Thus, we suggest that agmatine has the ability to activate imidazoline I-2R on peripheral arterioles. As additional evidence, vasodilatation induced by agmatine at maximal dose was enhanced by cotreatment with amiloride. It means that amiloride has the ability to produce vasodilatation regardless of the total activation of imidazoline receptors. Amiloride seems not effective on imidazoline receptors only. Another mechanism for action of amiloride shall be investigated in the future.

5. Conclusion

According to the obtained data, we suggest that agmatine may act as peripheral antihypertensive agent through activation of imidazoline I-2 receptor for vasodilatation mainly through open of KATP channel. Thus, agmatine-like compound but not based on guanidinium structure has the potential to develop as a new antihypertensive agent in the future.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Authors’ Contribution

Mei-Fen Chen and Jo-Ting Tsai contributed equally to this work.

Acknowledgments

The authors thank Mr. K.F. Liu for technical assistance and Professor King-Pong Lin for kind support and direction in this study. The present study was supported mainly by the Grants of National Sciences Council (NSC 102-2911-I-002-303 and NSC 103-2911-I-002-303).

References

  1. D. L. Cohen and R. R. Townsend, “Update on pathophysiology and treatment of hypertension in the elderly,” Current Hypertension Reports, vol. 13, no. 5, pp. 330–337, 2011. View at Publisher · View at Google Scholar · View at Scopus
  2. S. Regunathan, C. Youngson, W. Raasch, H. Wang, and D. J. Reis, “Imidazoline receptors and agmatine in blood vessels: a novel system inhibiting vascular smooth muscle proliferation,” The Journal of Pharmacology and Experimental Therapeutics, vol. 276, no. 3, pp. 1272–1282, 1996. View at Google Scholar · View at Scopus
  3. J. Yang, W.-Z. Wang, F.-M. Shen, and D.-F. Su, “Cardiovascular effects of agmatine within the rostral ventrolateral medulla are similar to those of clonidine in anesthetized rats,” Experimental Brain Research, vol. 160, no. 4, pp. 467–472, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. P. Ernsberger, M. E. Graves, L. M. Graff et al., “I1-imidazoline receptors. Definition, characterization, distribution, and transmembrane signaling,” Annals of the New York Academy of Sciences, vol. 763, pp. 22–42, 1995. View at Publisher · View at Google Scholar · View at Scopus
  5. N. G. Morgan, S. L. Chan, M. Mourtada, L. K. Monks, and C. A. Ramsden, “Imidazolines and pancreatic hormone secretion,” Annals of the New York Academy of Sciences, vol. 881, pp. 217–228, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. T.-N. Lui, C.-W. Tsao, S.-Y. Huang, C.-H. Chang, and J.-T. Cheng, “Activation of imidazoline I2B receptors is linked with AMP kinase pathway to increase glucose uptake in cultured C2C12 cells,” Neuroscience Letters, vol. 474, no. 3, pp. 144–147, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. C.-H. Chang, H.-T. Wu, K.-C. Cheng, H.-J. Lin, and J.-T. Cheng, “Increase of β-endorphin secretion by agmatine is induced by activation of imidazoline I2A receptors in adrenal gland of rats,” Neuroscience Letters, vol. 468, no. 3, pp. 297–299, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. P. A. van Zwieten and S. L. Peters, “Central I1-imidazoline receptors as targets of centrally acting antihypertensive drugs. Clinical pharmacology of moxonidine and rilmenidine,” Annals of the New York Academy of Sciences, vol. 881, pp. 420–429, 1999. View at Publisher · View at Google Scholar · View at Scopus
  9. M. Esler, “High blood pressure management: potential benefits of I1 agents,” Journal of Hypertension, vol. 16, no. 3, pp. S19–S24, 1998. View at Google Scholar · View at Scopus
  10. G.-Y. Mar, M.-T. Chou, H.-H. Chung, N.-H. Chiu, M.-F. Chen, and J.-T. Cheng, “Changes of imidazoline receptors in spontaneously hypertensive rats,” International Journal of Experimental Pathology, vol. 94, no. 1, pp. 17–24, 2013. View at Publisher · View at Google Scholar
  11. C. Dardonville and I. Rozas, “Imidazoline binding sites and their ligands: an overview of the different chemical structures,” Medicinal Research Reviews, vol. 24, no. 5, pp. 639–661, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. F. L. Wynne, J. A. Payne, A. E. Cain, J. F. Reckelhoff, and R. A. Khalil, “Age-related reduction in estrogen receptor-mediated mechanisms of vascular relaxation in female spontaneously hypertensive rats,” Hypertension, vol. 43, no. 2, pp. 405–412, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. W. Raasch, U. Schäfer, F. Qadri, and P. Dominiak, “Agmatine, an endogenous ligand at imidazoline binding sites, does not antagonize the clonidine-mediated blood pressure reaction,” British Journal of Pharmacology, vol. 135, no. 3, pp. 663–672, 2002. View at Google Scholar · View at Scopus
  14. F. Messerli, “Moxonidine: a new and versatile antihypertensive,” Journal of Cardiovascular Pharmacology, vol. 35, no. 4, pp. S53–S56, 2000. View at Publisher · View at Google Scholar · View at Scopus
  15. E. B. Monroy-Ordoñez, C. M. Villalón, L. E. Cobos-Puc, J. A. Márquez-Conde, A. Sánchez-López, and D. Centurión, “Evidence that some imidazoline derivatives inhibit peripherally the vasopressor sympathetic outflow in pithed rats,” Autonomic Neuroscience: Basic & Clinical, vol. 143, no. 1, pp. 40–45, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. R. A. Khalil, “Modulators of the vascular endothelin receptor in blood pressure regulation and hypertension,” Current Molecular Pharmacology, vol. 4, no. 3, pp. 176–186, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. G. V. Guinea, J. M. Atienza, F. J. Rojo et al., “Factors influencing the mechanical behaviour of healthy human descending thoracic aorta,” Physiological Measurement, vol. 31, no. 12, pp. 1553–1565, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. T. M. Cocks, P. J. Little, J. A. Angus, and E. J. Cragoe Jr., “Amiloride analogues cause endothelium-dependent relaxation in the canine coronary artery in vitro: possible role of Na+/Ca2+ exchange,” British Journal of Pharmacology, vol. 95, no. 1, pp. 67–76, 1988. View at Google Scholar · View at Scopus
  19. J. Y. Sung and H. C. Choi, “Metformin-induced AMP-activated protein kinase activation regulates phenylephrine-mediated contraction of rat aorta,” Biochemical and Biophysical Research Communications, vol. 421, no. 3, pp. 599–604, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. M.-F. Chen, J.-T. Tsai, L.-J. Chen et al., “Antihypertensive action of allantoin in animals,” BioMed Research International, vol. 2014, Article ID 690135, 6 pages, 2014. View at Publisher · View at Google Scholar
  21. S. Diamant, T. Eldar-Geva, and D. Atlas, “Imidazoline binding sites in human placenta: evidence for heterogeneity and a search for physiological function,” British Journal of Pharmacology, vol. 106, no. 1, pp. 101–108, 1992. View at Google Scholar · View at Scopus
  22. T. Miura, Y. Liu, M. Goto et al., “Mitochondrial ATP-sensitive K+ channels play a role in cardioprotection by Na+–H+ exchange inhibition against ischemia/reperfusion injury,” Journal of the American College of Cardiology, vol. 37, no. 3, pp. 957–963, 2001. View at Publisher · View at Google Scholar · View at Scopus
  23. W. I. Rosenblum, E. P. Wei, and H. A. Kontos, “Vasodilation of brain surface arterioles by blockade of Na–H+ antiport and its inhibition by inhibitors of KATP channel openers,” Brain Research, vol. 1005, no. 1-2, pp. 77–83, 2004. View at Publisher · View at Google Scholar · View at Scopus
  24. E. A. Ko, J. Han, I. D. Jung, and W. S. Park, “Physiological roles of K+ channels in vascular smooth muscle cells,” Journal of Smooth Muscle Research, vol. 44, no. 2, pp. 65–81, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. J. E. Brayden, “Functional roles of KATP channels in vascular smooth muscle,” Clinical and Experimental Pharmacology and Physiology, vol. 29, no. 4, pp. 312–316, 2002. View at Publisher · View at Google Scholar · View at Scopus
  26. N. Nakhostine and D. Lamontagne, “Adenosine contributes to hypoxia-induced vasodilation through ATP- sensitive K+ channel activation,” American Journal of Physiology: Heart and Circulatory Physiology, vol. 265, no. 4, pp. H1289–H1293, 1993. View at Google Scholar · View at Scopus
  27. M. Ghosh, S. T. Hanna, R. Wang, and J. R. McNeill, “Altered vascular reactivity and KATP channel currents in vascular smooth muscle cells from deoxycorticosterone acetate (DOCA)-salt hypertensive rats,” Journal of Cardiovascular Pharmacology, vol. 44, no. 5, pp. 525–531, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. C.-C. Tsai, T.-Y. Lai, W.-C. Huang, I.-M. Liu, and J.-T. Cheng, “Inhibitory effects of potassium channel blockers on tetramethylpyrazine-induced relaxation of rat aortic strip in vitro,” Life Sciences, vol. 71, no. 11, pp. 1321–1330, 2002. View at Publisher · View at Google Scholar · View at Scopus
  29. K.-L. Wong, P. Chan, H.-Y. Yang et al., “Isosteviol acts on potassium channels to relax isolated aortic strips of Wistar rat,” Life Sciences, vol. 74, no. 19, pp. 2379–2387, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. W. Raasch, U. Schäfer, J. Chun, and P. Dominiak, “Biological significance of agmatine, an endogenous ligand at imidazoline binding sites,” British Journal of Pharmacology, vol. 133, no. 6, pp. 755–780, 2001. View at Google Scholar · View at Scopus
  31. S.-L. Hwang, I.-M. Liu, T.-F. Tzeng, and J.-T. Cheng, “Activation of imidazoline receptors in adrenal gland to lower plasma glucose in streptozotocin-induced diabetic rats,” Diabetologia, vol. 48, no. 4, pp. 767–775, 2005. View at Publisher · View at Google Scholar · View at Scopus