Changes on Sugar and Starch Contents during Seed Development of Synergistic Sweet Corn and Implication on Seed Quality

Harakotr, Bhornchai; Sutthiluk, Warisa; Rithichai, Panumart

doi:https://doi.org/10.1155/2022/6550474

International Journal of Agronomy

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2022 | Article ID 6550474 | https://doi.org/10.1155/2022/6550474

Changes on Sugar and Starch Contents during Seed Development of Synergistic Sweet Corn and Implication on Seed Quality

Bhornchai Harakotr,¹Warisa Sutthiluk,¹and Panumart Rithichai¹

Academic Editor: Othmane Merah

Received22 Oct 2021

Revised10 Feb 2022

Accepted11 Feb 2022

Published07 Mar 2022

Abstract

Synergistic sweet corn equipped with multiple-recessive genes encoding sugar synthesis is proposed through hybrid breeding to improve the balance eating quality including flavor, texture, and aroma. However, the drawback on seed quality occurs such as low germination and poor seedling vigor. This study aimed to investigate the changes of carbohydrate contents on seed quality of five sweet corn genotypes differing in the number of equipped recessive genes during seed development. Seeds were sampled at 4-day intervals from 18 to 46 days after pollination (DAP) and analyzed for seed germination, sugar, water-soluble polysaccharide, and starch. Then, their relationships were analyzed by using time series regression analysis. Although there were significant differences among 5 corn genotypes in their seed germination and carbohydrate contents, some genotypes showed responses in similar patterns. The optimal time to harvest seeds was genotype-dependent, which were 38 DAP for triple-recessive gene (btbtsh₂sh₂wxwx) and single-recessive genes (BtBtsh₂sh₂WxWx and Sh₂Sh₂susuWxWx) and 42 DAP for double-recessive genes (BtBtsh₂sh₂wxwx). The regression analysis revealed that seed germinability could be predicted by total starch content in synergistic sweet corn lines during seed development stages; however, this prediction seemed to be negligible in sweet corn genotypes equipped with a single-recessive gene. Implications and further suggestions for establishing an effective seed production technique and seed quality of synergistic sweet corn are discussed.

1. Introduction

Sweet corn (Zea mays L. saccharata) is a vegetable crop widely cultivated and consumed all over the world as it possesses good flavor, tenderness, and aroma that appeal to consumer preferences [1]. This crop is also rich in nutritional values, minerals, and antioxidants, benefiting human health [2, 3]. Nowadays, the volume of Thai sweet corn export is expanding due to competition on the price and quality of sweet corn. In 2019, the total harvest area of Thai sweet corn occupies more than 80,198 hectares, yielding 501,242 tons [4]. However, the sweet corn producers and industries require more reliable cultivars that could be accomplished by hybrid breeding with high seed qualities [2].

Sweet corn can be classified into five types based on the gene compositions in the endosperm: normal (sugary gene, su₁), sugary enhanced (sugary enhanced gene, se), super sweet (shrunken gene, sh₂, or brittle gene, bt₁), synergistic, and augmented shrunken genotypes [5]. Synergistic corn equipped with su₁, sh₂, and se combinations gives a balance eating quality between high sugar content and tenderness [6, 7]. Moreover, these recessive genes encoding sweetness have been utilized to improve the eating quality of traditional waxy corn through sweet-waxy corn hybrids [7–10]. However, the adverse effects on seed qualities were found in sweet corn genotypes with two or more recessive genes controlling sugar synthesis. Pairochteerakul et al. [11] reported that incorporations of either bt₂ or sh₂ gene into wx background resulted in low germination percentage and poor seedling vigor. This situation would be unfavorable for hybrid seed production. Several approaches have been proposed to address that issue by using either conventional or molecular breeding, seed production techniques, and seed enhancement technologies [12–14].

Seed vigor is essential to enhance a rapid, uniform seed emergence under various field conditions and to extend seed storability; thus, this parameter is commonly included in routine seed testing [13, 15]. Vigorous hybrid seeds demonstrating rapid germination, high germination percentage, and uniform seedling growth are associated with high potential yield in crop production [15, 16]. Being one of the complex agronomic traits, seed vigor is a manifestation of genetics, environmental conditions during seed development, dates of harvest, mechanical handlings, dryings, storage conditions, storage periods, and storage insect pests [17–19]. In this context, the seed developmental stage is critical affecting seed vigor and correlated with the timing of harvest or seed maturation stage [17]. In sweet corn genotypes, alterations of seed germination and vigor during seed development and maturation correspond to any changes in seed morpho-physiological traits [20–22]. Seed storage reserves including sugar and starch are another key factor influencing seed vigor because these carbohydrates are converted to soluble sugars as energy for initial seed germination and seedling growth [23]. During seed development, the concentrations of hexose sugars (glucose, fructose, glucose 6-phosphatase, and fructose 6-phosphatase) are progressively declining whereas storage carbohydrates such as sucrose, starch, and raffinose family oligosaccharides (mainly raffinose, stachyose, and verbascose) are increasing [24]. A previous study noticed that kernel carbohydrate profiles of sweet corn genotypes at the maturity stage had an impact on seed quality [11, 25]; however, such investigation at each seed development of synergistic sweet corn genotypes has not been reported. Here, we analyzed the relationship between carbohydrate characters and seed germination across the seed developmental stages of synergistic sweet corn genotypes differing in the number of equipped recessive genes by using the time series regression technique. Our findings suggested that carbohydrate characters should be considered to optimize the seed qualities of synergistic sweet corn in the seed production technology.

2. Materials and Methods

2.1. Seed Materials and Field Experiment

Four sweet corn inbred lines (F₇ generation) and one sweet corn F₁ hybrid differing in genes controlling endosperm carbohydrate characters were used in this study (Table 1). Inbred line 101TSC-10 has triple-recessive genes (btbtsh₂sh₂wxwx) whereas C4 has double-recessive genes (BtBtsh₂sh₂wxwx). Both lines are synergistic sweet corn, screened by both visual selection and microsatellite markers during 2014–2017 [11]. Inbred lines TSC/H3-7 and P4546/Delec-1 have single-recessive allele sh₂ (BtBtsh₂sh₂WxWx) and su (Sh₂Sh₂susuWxWx), respectively. Dr. Pek’s 1351 is a popular super sweet corn hybrid (BtBtsh₂sh₂WxWx) variety from Sweet Seeds Co. Ltd.

Each genotype was planted at the Department of Agricultural Technology, Thammasat University, Pathum Thani, Thailand, in the dry season of 2019/2020 (November 2019–February 2020). This site had loamy sand soils with a pH of 4.84, an electrical conductivity of 0.94 dS·m⁻¹, and 0.31 mg·kg⁻¹ N, 110.95 mg·kg⁻¹ P, and 34.00 mg·kg⁻¹ K. Each genotype was grown in 10 rows and 5 m in length, with a spacing of 0.75 m between rows. The distance between hills in a row was 0.25 m; hence, the population density was 5.33 plants m⁻². The Department of Agriculture, Thailand, recommendations [26] were followed for field management. Before planting, fertilizer formula 15-15-15 of NPK was applied at a rate of 312.5 kg·ha⁻¹, and 156.25 kg·ha⁻¹ of fertilizer formula 46-0-0 was applied at 20 and 40 days after planting. Weed control was accomplished by hand weeding at critical periods of crop whereas pesticides were applied when the crop reached the economic injury level. Hand-pollination was performed on each genotype to avoid unintended pollen contamination.

2.2. Seed Harvest

Sweet corn seed is ready to harvest at about 30–40 days after pollination (DAP), but the seed starts acquiring germinability at 14 DAP [15]. Twenty hand-pollinated ears of each genotype were sampled randomly at the 4-day intervals from 18 to 46 DAP to monitor the seed development at different stages. Immediately after sampling at each harvest interval, 200 seeds of the middle ear part were shelled by hand for dual purposes. For the first purpose, 100 seeds were frozen in liquid nitrogen to stop all metabolic activities. Then, samples were dried with lyophilization and stored at −20°C for subsequent chemical analyses. For the second purpose, 100 seeds were used to determine seed moisture content and 100-seed dry weight. The remaining corn seeds within the ear were dried at 35°C until constant moisture content, and the seeds were then threshed from the cob. The dried seeds were stored in sealed containers at 15°C and 50% humidity for further seed quality tests.

2.3. Seed Moisture Content and 100-Seed Dry Weight

Seed moisture content was determined using International Seed Testing Association (ISTA) method [27]. One hundred kernels per sample were dried for 24 h in a drying oven with a temperature set at 103°C. The samples were kept in a desiccator to cool down for 30–45 min, and then the weights were recorded as seed dry weight. Seed moisture was calculated as follows:where f is the kernel moisture content (%); M₁ is the weight (g) of the used container and its cover; M₂ is the weight (g) of the container, its cover, and the kernels before drying in the oven; and M₃ is the weight (g) of the container, its cover, and the seeds after drying in the oven. Each genotype sample was measured with four replicates.

2.4. Seed Quality Tests

Standard germination tests [27] were performed using four replicates per sample with 50 seeds per replicate. Seeds of each sample were placed in germination boxes (12 × 18 × 9 cm). For the growing media, peat moss was added and moistened with water to 9% (w/w) [22, 23]. The boxes were stored in a room under constant temperature at 25°C for 7 days. Germinated seeds were counted daily. After 7 days, the germination percentage was calculated as the percentage of normal germinated seeds to all tested seeds at the end of the full tests [28].

Based on the number of germinated seeds after 7 days, the germination index was calculated as follows:where G_t is the germinated seeds on day t and T_t is the time corresponding to G_t in days. Mean germination time was calculated as follows:where N_i is the number of germinated seeds on day i and T_i is the time after sowing.

An electrical conductivity test was carried out with four replicates. Each replicate derived from 25 seeds was sampled for 0.01 g and was soaked in a beaker (200 mL) containing 150 mL distilled water at 20°C for 24 h [27]. After soaking, the EC value was determined using a conductivity meter (ver. HI98311, Hanna instruments, RI, USA) with an electrode with constant 1.

2.5. Seed Carbohydrate Composition Analyses

Soluble sugar contents were extracted and determined by using the phenolic-sulfuric acid method [25]. Briefly, the samples were ground with blender, then ground samples were sieved through a 63 μm sieve. Fifty milligrams of samples were extracted with 3 mL of 80% aqueous ethanol in a glass tube, and the extract was heated at 65°C for 1 h to obtain the soluble sugar. The supernatants were then moved to new glass tubes containing ethanol, and the residual was further extracted twice. The final extracts derived from ethanol solutions were adjusted to 9 mL and filtered through a 0.45 μm filter (Millipore Corp., MA, USA) for sugar quantification. Reversed-phase HPLC analysis of mono- and disaccharide was performed using a Shimadzu system (Shimadzu, Japan) equipped with a binary pump (LC-20AC pump) and a refractive index detector (RID-10A). Chromatographic separations were performed on a Sugar-Pak I column (6.5 × 300 mm, i.d. 10 μm) (Waters, MA, USA). The mobile phase was deionized water at the flow rate of 0.5 ml/min. Column operating conditions were as follows: temperature 80°C and injection volume of 10 μl. Different dilutions of individual external standards ranging from low to high concentrations were made, and standard curves were plotted. The results of individual sugar contents were expressed as milligram per gram of dry weight (mg g⁻¹ dry weight). Total sugar content was calculated from the sum of each sugar fraction content.

Three parameters including phytoglycogen, amylopectin, and total starch contents were also determined by using the phenolic-sulfuric acid method [25]. Each dried sample was isolated from the native starches with four replications. To inactivate the enzymes, 100 g of each sample was soaked in 0.16% sodium hydrogen sulfite at 50°C for 24 h. Immediately after soaking, the sample was ground in the blender and then sieved through a 105 μm sieve-mesh. The slurry ground samples were put in toluene alcohol for native starch separation and were dried at 80°C for 24 h. For starch extraction, 50 mg of native starch powder was extracted with 80% ethanol and then heated at 80°C for 1 h. Phytoglycogen and total starch contents were measured at the wavelength of 490 and 600 nm, respectively (Shimadzu mod. UV-128, Japan). The results of phytoglycogen and total starch contents were expressed as milligram per gram of dry weight (mg g⁻¹ dry weight).

Amylopectin content was measured according to Simla et al. [25]. Twenty grams each of a series of pure amylose and amylopectin (ranging from 0 to 100% amylopectin) were dissolved in 10 mL of 90% dimethyl sulfoxide. Then, 1 mL of the diluted solution was mixed with 1 mL iodine solution and adjusted to 10 mL with distilled water. The solution was stood for 15 min for color establishment. The absorbance of the standard mixture at 600 nm was measured (Shimadzu mod. UV-128, Japan). The result of total amylopectin content was expressed as milligram per gram of dry weight (mg g⁻¹ of dry weight).

2.6. Statistical Analysis

Analysis of variance (ANOVA) was subjected to all parameters. The least significant difference (LSD) was used for mean comparison at 0.01 probability level [29]. These analyses were done using the Statistix program (ver. 10.0, Analytical Software, FL, USA). Regression equations between seed carbohydrate profiles and seed germination were conducted by using SPSS software (ver. 26, IBM Crop., NY, USA). The graphs were then plotted by Microsoft excel (ver. 2016, Microsoft, WA, USA).

3. Results and Discussion

Normally, seed development can be distinguished into three stages as follows: stage I, rapid cell division; stage II, rapid synthesizing of storage materials; and stage III, the little synthesis of storage material and dehydration [30]. The terminal stage of seed development called the maturation stage occurred between 30 and 40 days after anthesis in sweet and waxy corn [12, 21, 23]. However, seeds gain the ability to germinate at 14 and 21 DAP for sweet and waxy corns, respectively [12, 22]. Our preliminary study found that all tested genotypes were not germinated at 14 DAP. Previous studies reported the low germination rates of sweet corn genotypes harvested at between 11 and 15 DAP because of inadequate seed endosperm [21, 31]. Therefore, the changes of seed germination and carbohydrate contents of single-, double-, and triple-recessive sweet corn genotypes from 18 to 46 DAP were evaluated in this study.

3.1. Changes in Seed Moisture Content and Seed Dry Weight during Seed Development

Physiological maturity greatly influenced by genetic and environmental factors has been recognized to determine the proper timing of harvest in seed production. Several criteria are commonly used to indicate physiological maturity in sweet corn including black layer formation, seed dry matter, seed moisture content, and milk line development [32, 33]. Among these parameters, kernel moisture content and seed dry weight accumulation after pollination are the simple methods to apply [32]. In general, the seed moisture content of all sweet corn genotypes was decreasing gradually along with their seed developments (Figure 1(a)). From 18 to 34 DAP, seed moisture content was declining ranging from 11% (Dr. Pek’s 1351) to 22% (101LTSC-10). The largest reductions of seed moisture content occurred at 38 DAP for all genotypes except for the C4 inbred line. Overall, C4 inbred line retained the highest seed moisture content among all genotypes throughout seed developments. Unlike seed moisture content, 100-seed weight was gradually increasing during seed development, reaching their maximum values at 34 DAP for 101LTSC-10, C4, THC/H3-7, and P4546/Delec-1 inbred lines and at 30 DAP for Dr. Pek’s 1351 hybrid (Figure 1(b)). At this harvest stage, the physiological maturity of each sweet corn genotype could be declared as the maximum 100-seed weight was noticed [34]. However, the seed moisture contents of all tested genotypes remained high ranging from 28.67 to 64.84%. This condition complicated the activities of mechanical shelling that requires relatively low seed moisture content below 35%. Besides, the balance between seed drying costs and maximum seed yield has normally been the major consideration for determining the proper timing of harvest in corn [32, 35]. Thus, three inbred lines 101LTSC-10, TSC/H3-7, and P4546/Delec-1 could be harvested during 38–46 DAP when their seed moisture contents were below 35%.

(a)

(b)

3.2. Changes of Seed Germination and Vigor during Seed Development

During seed developmental stages, seed germination percentage, germination index, mean germination time, and EC value of five corn genotypes showed different patterns, and some genotypes responded the similar patterns (Figure 2). Rapid increases in germination percentage were observed during 18–38 DAP in genotypes TSC/H3-7, 101LTSC-10, and P4546/Delec-1. However, germination percentage seemed to be stable during 38–46 DAP in genotype TSC/H3-7, but gradual declines were noticed in genotypes 101LTSC-10 and P4546/Delec-1 (Figure 2(a)). A similar pattern, C4 inbred line had the highest germination percentage at 42 DAP, and then the value was decreasing. Seed producers should harvest sweet corn seeds at their optimal maturity date since the postponement of seed harvest contributed to the significant loss of their germinability [33, 34]. The germination index of each genotype was gradually increasing during seed development, and these values reached the optimum point during 34–42 DAP (Figure 2(b)). On the contrary, mean germination time and EC value were gradually decreasing from 18 to 38 DAP and were increasing afterwards for all genotypes except for Dr. Pek’s 1351 hybrid (Figures 2(c)–2(d)).

(a)

(b)

(c)

(d)

Previous reports could not clearly elucidate the optimal harvest time for seed production purposes in sweet corn since different hybrids, growing locations, and maturity indices were used [17]. Seed harvest should be performed at the proper seed maturity stage that resulted in high seed vigor. Therefore, understanding the traits associations is essential. In this study, germination percentage, germination index, mean germination time, and EC value were used as a part of parameter indices to predict sweet corn seed vigor at different maturity stages. Seed vigor of the C4 inbred line was distinguishable from other tested genotypes, indicating that seed vigor was strongly influenced by genotypic background. Therefore, seed vigor together with high parental seed yield could be included in the selection criteria to achieve affordable hybrid seed production. In the present study, although there were obvious differences in seed vigor parameters from 30 to 46 DAP, 38 DAP was considered as the proper harvest timing for genotypes 101LTSC-10, THC/H3-7, and P4546/Delec-1. The other two genotypes C4 inbred line and Dr. Pek’s 1351 hybrid were suitable to harvest at 42 DAP. Genotype 101LTSC-10 with triple-recessive genes showed the highest germination percentage and germination index yet low mean germination time and EC value. Besides, inbred lines with double-recessive genes had the lowest seed germination and vigor. The result reflected that seed vigor was sensitive to the genetic background. This corroborated a previous study by Pairochteerakul et al. [11], who found that the btbtsh₂sh₂wxwx sweet corn genotype had a higher germination percentage than double-recessive genotypes containing the wx gene. However, they could not clearly propose the optimal maturity date because thorough assessments on these parameters during each seed developmental stage in sweet corn genotypes were not carried out. Low germination of inbred lines with double-recessive genes could be addressed by seed priming and seedling nursery prior to transplanting in hybrid seed production [11, 12]. Two single-recessive genotypes TSC/H3-7 (sh₂sh₂) and P4546/Delec-1 (susu) were not different in seed vigor in contrast to Zhao et al. [36] reporting that sweet corn with the sh₂ gene had higher sugar content than sweet corn with the se gene. Moreover, they noticed that microorganisms could more severely infect the high sugar sweet corn genotype resulting in volatile aldehyde production, which is harmful to seed germination and seedlings. This difference might also be caused by other genetic backgrounds and growing conditions. From our study, it is interesting to note that the EC value is strongly associated with seed quality, suggesting that the EC value is an efficient indicator for seed vigor of sweet corn. This parameter was also suitable for sweet corn [36, 37] and other crops including pea [38], sorghum [39], and safflower [40]. However, Zhao et al. [36] reported that the EC test was only reliable to estimate the seed vigor of sweet corn with the se gene.

3.3. Changes of Sugar and Starch Contents in Sweet Corn Seeds during Seed Development

At an immature stage, the type of sweet corn (inbred line or hybrid) did not determine the carbohydrate compositions, but the differences in these characters were mainly genotype-dependent. Inbred line (TSC/H3-7) and hybrid cultivar (Dr. Pek’s 1351) with a single-recessive genotype (sh₂sh₂) had higher sugar content than P4546/Delec-1 (susu) and double- or triple-recessive gene genotypes (Figures 3(a)–3(d)). P4546/Delec-1 (susu) had higher phytoglycogen content than other genotypes (Figure 4(a)). This result indicated that sh₂ sweet corn genotypes could retain high contents of sugar fractions whereas su genotypes retained high phytoglycogen content. As a comparison, mutant (sugary-1, su1) crops, including corn and rice, showed relatively high phytoglycogen content [41, 42]. Meanwhile, double- and triple-recessive gene combinations resulted in the highest amylopectin and total starch contents during seed development (Figures 4(b) and 4(c)). Ketthaisong et al. [43] and Simla et al. [25] reported that wx corn genotypes comprise more than 80% amylopectin starch.

(a)

(b)

(c)

(d)

(a)

(b)

(c)

The types of reserves accumulated in the endosperm of developing seeds have many potential roles such as seed establishment and autotrophic developmental stage that foster initial seedling survival under various field conditions [24]. The changes in carbohydrate compositions and concentrations in sweet corn and other corn types during seed development have been reported [24, 44]. However, changes in sugar and starch contents during seed developmental stages of synergistic sweet corn genotypes have not been reported. In the present study, five sweet corn genotypes differed in their seed carbohydrate characters including sucrose, glucose, fructose, total sugar, phytoglycogen, amylopectin, and total starch (Figure 3). These variations on seed carbohydrate profiles contributed to the seed vigor, and the optimal harvest time of each sweet corn genotype was different. Sucrose was the most abundant soluble saccharide among all sugar fractions of the endosperm. This fraction peaked at 18 DAP for three genotypes C4, THC/H3-7, and Dr. Pek’s 1351 and at 22 DAP for two inbred lines 101LTSC-10 and P4546/Delec-1 (Figure 3(a)). After the endosperm sucrose reached the maximum values, this content rapidly decreased from milk (18–20 DAP) to physiological maturity stages. However, these reductions varied depending upon genotype. Glucose, fructose, and total sugar contents were likewise decreasing after surpassing the maximum values (Figures 3(b)–3(d)). This finding confirmed that the contents of both total sugar and sugar fractions started increasing at a post-pollination stage, reached the peak values at the milk stage, started decreasing afterwards, and were at the lowest values at the physiological maturity stage [45, 46]. However, incorporation of the wx recessive gene into synergistic sweet corn backgrounds (BtBtsh₂sh₂wxwx and btbtsh₂sh₂wxwx) resulted in low sugar contents during most seed developmental stages. In contrast to our finding, Pairochteerakul et al. [11] reported that sweet corn genotypes with BtBtsh₂sh₂wxwx background showed high sugar contents at immature and mature stages than genotypes btbtsh₂sh₂wxwx and BtBtsh₂sh₂WxWx. This opposite finding might be due to different experimental conditions and even the methods of sugar quantification.

In contrast to sugar, the starch contents of all genotypes were increasing following the prolonged harvest period. Rapid increases in phytoglycogen were observed during 18–46 DAP in the C4 inbred line (Figure 4(a)). On the contrary, the phytoglycogen of genotypes 101LTSC-10, C4, THC/H3-7, P4546/Delec-1, and Dr. Pek’s 1351 was slightly increased from 18 to 30 DAP, and the rapid increment was noticed afterwards. Both amylopectin and total starch contents of sweet corn genotypes followed a similar pattern of their phytoglycogen (Figures 4(b) and 4(c). Sugar gradually converted to starch during the development of kernel endosperm [21]. These changes were caused by the activities of key starch biosynthetic enzymes including UDP-glucose pyrophosphorylase, ADP-glucose pyrophosphorylase, hexokinase, granule bound starch synthase, and soluble starch synthase during corn endosperm formation [47, 48]. Starch biosynthetic enzyme activities were rapidly increasing after 12 DAP and reached the maximum levels at 20–22 DAP [48]. Both previous reports and our result confirmed that the contents of carbohydrate characters during seed development were based on genes controlling endosperm.

3.4. Regression Analysis between Carbohydrate Characters and Seed Quality during Seed Development of Sweet Corn Genotypes

Time series regression (TSR) analysis has been applied to assess the seed quality of sweet corn by considering seed size and endogenous polyamines [15, 22]. In our study, seed carbohydrate characters were included in TSR analysis to estimate the seed quality of five sweet corn genotypes from 18 to 46 DAP. Since phytoglycogen did not contribute to the equation, this variable was then omitted for the analysis. Carbohydrate contents contributed to the seed germination of synergistic sweet corn lines; however, no distinctive regression relationship was observed between carbohydrate contents and seed germination in any single-recessive gene genotype tested in this study. Among eight equations of triple- and double-recessive genes, X₆ (total starch content) in 7 equations, X₅ (amylopectin) in 4 equations, X₁ (sucrose) in 3 equations, and X₂ (glucose), and X₃ (fructose) in 2 equations had a significant contribution to the corresponding equations, whereas X₄ (total sugar content) did not contribute to all equations (Table 2). The total starch content of the significant equations was 75% more contributed than the other 5 variables. The equations with significant starch content had a regression coefficient (R²) ranging from 0.717 to 0.993, suggesting that the equations had a stronger responsibility level with data, and total starch content was the key factor affecting seed germination and vigor. Simpler equations were developed by using TSR when some factors with minor effects on seed germination were eliminated (Table 3). Total starch content had significant contributions () to all equations. Although regression coefficient values slightly changed, these equations were easier to be used. Moreover, all coefficients of total starch were positive to estimate germination percentage and germination index, indicating that seed vigor could be predicted by the total starch content during seed developmental stages in synergistic sweet corn and that vigorous seeds would have a higher total starch content. Besides, we utilized the TSR equations to classify carbohydrate accumulation during seed development. This corroborated previous reports that modeled the seed developmental process by the TSR’s equation [15, 21, 22]. However, seed vigor is a complex agronomic trait that is necessary to be determined the factors as does the effect of the genetic and biochemical processes studied during seed development. Moreover, the seed industry needs a rapid and nondestructive evaluation method for seed germination. This will be the subject of further investigations.

4. Conclusions

Genes controlling endosperm altered carbohydrate characters and seed vigor during seed development of sweet corn genotypes. Proper harvest date for triple-recessive genotype (btbtsh₂sh₂wxwx) and single-recessive genotypes (BtBtsh₂sh₂WxWx and Sh₂Sh₂susuWxWx) was 38 DAP whereas that for double-recessive genotype (BtBtsh₂sh₂wxwx) was 42 DAP. The TSR analysis was effective to predict germination percentage and germination index when total starch contents were positive. Moreover, total starch content was the optimal index for assessing the seed germination and vigor of synergistic super sweet corn inbred lines.

Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Conflicts of Interest

The authors declare that there are no conflicts of interest.

Acknowledgments

The authors gratefully acknowledge the financial support provided by the Faculty of Science and Technology, Thammasat University (grant no. SciGR24/2563). Acknowledgment is also extended to the Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University and Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University for providing research facilities, and Mr. Abil Dermail for proofreading the manuscript.

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