The aim of this study was to prepare fast disintegrating combination tablet of taste masked Levocetrizine dihydrochloride and Montelukast sodium by using direct compression method. To prevent bitter taste and unacceptable odour of the Levocetrizine dihydrochloride drug, the drug was taste masked with ion exchange resins like Kyron-T-104 and Tulsion-412. Among the two resins, Kyron-T-104 was selected for further studies because of high drug loading capacity, low cost, and better drug release profile. An ion exchange resin complex was prepared by the batch technique and various parameters; namely, resin activation, drug: resin ratio, pH, temperature, and stirring time, and swelling time were optimized to successfully formulate the tasteless drug resin complex (DRC). The tablets were prepared using microcrystalline cellulose (MCC) PH 102 as diluent along with crospovidone (CP), croscarmellose sodium (CCM), and sodium starch glycolate (SSG) as a superdisintegrants. The tablets were evaluated for weight variation, hardness, friability, wetting time, water absorption ratio, disintegration time (DT), and dissolution study and it was concluded that the tablet formulation prepared with 2% SSG + CCS showed better disintegration time in comparison with other formulation and good drug release. The stability studies were carried out for the optimized batch for three months and it showed acceptable results.

1. Introduction

Various physiological and neurological conditions like dysphagia, motion sickness, and hand tremors lead to noncompliance of conventional oral dosage forms. Mouth dissolving drug delivery systems (MDDDS), orally disintegrating system (ODT), and fast disintegrating tablet (FDT) are especially designed for dysphagic, geriatric, pediatric, bed-ridden, travelling, and psychotic patients who are unable to swallow or refuse to swallow conventional oral formulations. As they dissolve/disintegrate very fast when placed in the mouth, FDT are the most convenient dosage forms for dysphagic, pediatric, and geriatric patients with swallowing problem [13]. They do not require water for administration and thus are a good alternative for travelers and for bed ridden patients. They simply vanish when placed in the mouth and so cannot be hidden in mouth by psychotic patients. These products not only increase the patient’s compliance but also fetch large revenues to manufacturers due to line extension of the existing formulation [46].

FDT or MDDDS display a fast and spontaneous deaggregation in the mouth, soon after it comes in contact with saliva, dissolving the active ingredient and allowing absorption through all possible membranes it comes in contact with during deglutition [79].

Recently, several new advanced technologies lyophilization, moulding, direct compression, cotton candy process, spray drying, sublimation, mass extrusion, nanonization, and quick dissolve film formation have been introduced for the formulation of mouth dissolving tablets (MDTs) or fast disintegrating system with very interesting features, like extremely low disintegration time, exceptional taste masking ability, pleasant mouth feel, and sugar free tablets for diabetic patients [10, 11]. These techniques are based on the principles of increasing porosity and/or addition of superdisintegrants and water soluble excipients in the tablets [1214].

Ion exchange resins have been increasingly used for the taste masking of bitter taste drugs and help to prepare fast disintegrating tablets. Thus, the taste masking of bitter active substances is a critical hurdle to overcome for the successful development of oral formulations [1517]. Levocetrizine dihydrochloride is an orally active and R-enantiomer of cetrizine and is a third generation, nonsedating selective peripheral H1-receptor antagonist used in seasonal allergic rhinitis, perennial allergic rhinitis, and chronic urticaria [18, 19]. Allergy is a common problem among all age groups. Levocetrizine dihydrochloride is rapidly absorbed after oral administration and half-life is 8.3 hr which makes it suitable for once a day formulation. These diseases require rapid onset of action in order to provide fast relief. Unfortunately, it is accompanied with a very unpleasant bitter taste so it requires taste masking [2022].

Montelukast sodium is a leukotriene receptor antagonist (LTRA) used in maintenance treatment of asthma and to relieve symptoms of seasonal allergies. It is usually administered orally [2325]. In the present study an attempt had been made to prepare fast disintegrating combination tablets of Montelukast sodium and taste masked Levocetrizine dihydrochloride for the treatment of allergic rhinitis using coprocessed superdisintegrants containing crospovidone, croscarmellose sodium, and sodium starch glycolate. The coprocessed superdisintegrants help to increase the water uptake with shortest wetting time and thereby decrease the disintegration time of the tablets. These systems may offer superior profile with potential mucosal absorption, thus increasing the drug bioavailability. These systems are also called mouth dissolving tablets, melt-in-mouth tablets, reprimelts, porous tablets, orodispersible, quick dissolving, or rapidly disintegrating tablets.

2. Materials and Methods

2.1. Materials

Montelukast sodium, sodium starch glycolate, croscarmellose sodium, and crospovidone were procured as gift sample from MMC Health care pvt., Ltd., Baddi, India. Levocetrizine dihydrochloride was a generous gift from Amol Pharmaceuticals, Jaipur, India. Tulsion-412 and Kyron-T-104 were obtained as gift samples from Cadila Pharmaceuticals, Ahemdabad, India. All other materials (microcrystalline cellulose PH 102, Magneshium stearate and Talc) and chemicals used were of analytical reagent grade.

2.2. Analysis of Levocetrizine Dihydrochloride and Montelukast Sodium

The solution containing 20 μg/mL of Levocetrizine dihydrochloride and Montelukast sodium in phosphate buffer (pH 6.8) was prepared and scanned over range of 200–400 nm against phosphate buffer (pH 6.8) as a blank using double beam UV spectrophotometer. The maximum wavelength was found to be 231.0 nm and 352.20 nm for Levocetrizine dihydrochloride and Montelukast sodium, respectively, which confirmed to the reported value.

2.3. Formulation of Drug (Levocetrizine Dihydrochloride): Resin Complex

Formulation of drug resin complex (DRC) of Levocetrizine dihydrochloride was done by the batch process; different amounts of resin Kyron-T-104 and Tulsion-412 were placed in beakers containing 100 mL of deionized water and allowed to swell for a definite period of time. Accurately weighed amount of Levocetrizine dihydrochloride (as per 1 : 1, 1 : 2, 1 : 3, 1 : 4, 1 : 5, 1 : 6, and 1 : 7 drug resin ratio) was added and stirred for desired period of time. The mixture was filtered and residue was washed with deionized water. Filtrate was analyzed by U.V. spectrophotometer at 231 nm for the unbound drug and percentage drug loading was calculated [2628].

2.4. Formulation Development of FDTS

All formulations of FDTs were prepared by direct compression technique for batch by taking DRC equivalent to 5 mg of Levocetrizine dihydrochloride, Montelukast sodium (10 mg), MCC was used as diluent, talc as an antiadherent, and magnesium stearate as a lubricant. All the ingredients were accurately weighed and blended together to get uniform mixture. Then the blend was compressed to get tablets using rotary tablet machine. The formulations are shown in Table 1.

3. Result and Discussion

3.1. Characterizations of Drugs, DRC, and Final Blend

The FTIR (Fourier transmission Infrared) spectroscopy study of Levocetrizine dihydrochloride, Montelukast sodium (mixed and separate), drug-resin complex, blend containing both the drug, resin, and other excipients used in the formulation development was carried out to check the compatibility to each other (Figures 1, 2, 3, and 4).

The spectra indicated that there was no drug-drug and drug-excipients interaction as the peaks of the drug and other excipients were seen the same in the drug-excipients mixture indicating that the drug molecule was present in an unchanged state in the formulation.

3.2. Precompression Evaluation
3.2.1. Optimization of Various Conditions for Maximum Drug Loading

Drug loading process was optimized for maximum drug loading considering conditions like effect of resin activation, drug: resin ratio, pH, temperature, resin swelling time, and stirring time [18, 2830].

Optimization of Resin Activation. Changing the ionic form of ion exchange resin (IER) might occasionally be required to convert a resin from one form to another, if it does not have the desired counter ions. Strongly acidic cation exchange resins are usually marketed in Na+ form and strongly basic anion exchange resins in Cl form. They are generally converted into hydrogen and hydroxide forms, respectively. The conversion could be achieved by soaking the resins with acid or alkali solutions, respectively. After changing the ionic form, the resin was subjected to washing with distilled water until elute becomes neutral in reaction and finally dried at 50°C. The effect of activation of resin on drug loading was studied. 100 mg of resin, placed on a Whatman filter paper in a funnel, was washed with deionized water and subsequently with 1 N HCl (100 mL). The resins were rewashed with deionized water until neutral pH was reached. DRC was prepared in the same way as discussed earlier using 100 mg each of Levocetrizine dihydrochloride and acid activated resins. Similarly, alkali activation of resin was done, replacing 1 N HCl with 1 N NaOH. Finally, Kyron T-104 and Tulsion-412 were also activated with combined treatment of 1 N HCl and 1 N NaOH solutions. Drug loading efficiency in each case was determined.

In the case of Kyron-T-104 the acid treated resin loaded maximum drug, that is, 67.24%, whereas 63.87% drug was loaded when Tulsion-412 used. The resin so activated exposed the exchangeable groups producing rapid ion exchange hence highest drug binding. Highest percentage drug loading was found for acid activated resin, but as compared to inactivated resin no major effect was found on percentage drug loading. Thus trials were made with inactivated resin. The results are shown in Table 2.

Optimization of Drug: Resin Ratio. 100 mg of Levocetrizine dihydrochloride was added to each of the fourteen beakers containing 100, 200, 300, 400, 500, 600, and 700 mg of resins separately swelled in 100 mL of deionized water. The mixture was stirred for 4 hrs. DRC was collected by filtration, washed with deionized water, and evaluated for drug content.

Optimization of pH. The study was carried out at six pH values 2, 3, 4, 5, 6, and 7. The pH was adjusted to desired value using standard solutions of HCl and NaOH. Loading efficiency was determined at these conditions.

The pH affects the extent of drug loading process. It was observed that optimum drug loading was achieved at pH 5.0 and was not much increased at pH higher than this. The results are shown in Table 3.

Optimization of Temperature. Temperature was optimized by preparing DRC using 100 mg Levocetrizine dihydrochloride and 300 mg resins in 100 mL of deionized water and set temperature at 20°C, 30°C, 40°C, 50°C, and 60°C using temperature controlled magnetic stirrer.

The Efficient drug loading on Kyron T-104 and Tulsion-412 occurred uniformly in the experimental temperature 30°C and the effect of temperature on drug loading is shown in Table 4.

Optimization of Resin Swelling Time. Optimization of resins swelling time was carried out by keeping 400 mg of resin Kyron T-104 and 500 mg of resin Tulsion-412 in each of the beakers containing 100 mL of deionized waterfor 30, 60, 90, and 120 min, respectively, on magnetic stirrer. DRC was prepared as described above using 100 mg of Levocetrizine dihydrochloride and percent drug loading was estimated.

It was noted that the resin requires proper swelling time for maximum drug loading. Swelling and hydration increase the rate and extent of ion exchange process. In unswollen resin matrix, the exchangeable groups are latent and coiled towards the backbone. Swelling increases the surface area and these groups get oriented towards outside. Loading that was considerably increased at 90 minutes was considered as the optimum swelling time. The effect of swelling time on drug loading is shown in Table 5.

Optimization of Resin Stirring Time. For optimizing stirring time, DRC was prepared by stirring 100 mg of Levocetrizine dihydrochloride with 400 mg of resin Kyron T-104 and 500 mg of resin Tulsion-412 in 100 mL of deionized water separately for 60, 90, 120, 180, 240, and 300 min and percent drug loading was evaluated.

Stirring time affects the ion exchange equilibrium process as it is stoichiometric process. This may indicate the significant involvement of Van-der Waals forces or chemisorptions taking place along with drug exchange during complexation. Loading was not considerably increased after 240 minutes so it was considered as the optimum contact time between Levocetrizine dihydrochloride and Kyron T-104 and Tulsion-412. The effect of stirring time on drug loading is shown in Table 6.

3.2.2. Evaluation of Taste of Resinate

Taste of resinate was checked by time intensity method. For this purpose human volunteers were selected. In this method a sample equivalent to a normal dose was held in mouth for 10 seconds and volunteers were asked to evaluate the taste of resinate. Bitterness levels were recorded immediately according Strong Biter, Moderate Bitter, Slight Bitter, and Tasteless. These volunteers were instructed not to swallow resinate, which were placed on the tongue. They were instructed to thoroughly gargle their mouth with distilled water after the completion of test [31].

Optimization of drug: resin ratio had shown that complete taste masking was achieved in ratio 1 : 4 to 1 : 7 in case of both the resins. The scale of bitterness is represented in Table 7.

Optimization of drug: resin ratio was done by taking inactivated resins in ratio 1 : 1 to 1 : 7 with drug (Levocetrizine dihydrochloride). Maximum drug loading was found in ratios 1 : 4 (kyron-T-104) and 1 : 5 (Tulsion-412) so further optimizations was done with this ratio. The effect of drug-resin ratio on drug loading and relation between drug-resin and drug loading is represented in Tables 8 and 9.

3.2.3. Micromeritic Properties

Prior to compression, the blend was evaluated for their micromeritic properties such as angle of repose, bulk density, tapped density, compressibility index, and Hausner’s ratio.

Angle of repose was determined by fixed funnel method (static method) the powder was poured in the funnel and the circumference of powder pile was drawn with a pencil on the graph paper and the radius of base of a pile was measured at five different points and average was taken for calculating angle of repose.

Both bulk and tapped density are determined in USP specification density apparatus by pouring the blend into a graduated cylinder via a large funnel and measure the volume and weight and tapped density was measured by operating the instrument for a fixed number of taps until powder has reached a minimum volume.

Hausner’s ratio indicates the flow ability and packing ability. When Hausner’s ratio is close to 1, materials have acceptable flow and packing ability.

From the results of precompression studies of the batch K1–K6, it was concluded that powder mixtures has good flow and compressibility property. The bulk density of powder mixtures was found in the range of 0.438–0.465 g/cm3. The values of Carr’s index were in the range of 12.89–13.49 and Hausner’s ratio was in the range of 1.142–1.156 suggested that blend having fairly good flow. The results of precompression evaluation are shown in Table 10.

3.3. Postcompression Evaluation

The weight variation test was carried out in order to ensure uniformity in the weight of tablets in a batch. The total weight of randomly selected 20 tablets was determined and the average was calculated. The individual weight of the tablets was also determined accurately and the weight variation was calculated.

The permissible limit for hardness is 3–12 kg/cm2. The hardness test was performed by using Pfizer hardness tester.

The thickness and diameter of the tablets were determined by using vernier calipers. Randomly 10 tablets selected were used for determination of thickness that expressed in mean ± SD and unit is millimeter (mm).

The pharmacopoeia limit of friability is 1% and friability was measured using a Roche friability apparatus, carried out at 25 rpm for 4 min (100 rotations). However, it becomes a great challenge for a formulator to achieve friability within this limit for MDT product keeping hardness at its lowest possible level in order to achieve a minimum possible disintegration time. The friability (%) is given by the following formula: where is weight of the tablets before the test and is the weight of the tablets after test.

Tablets prepared by direct compression method were found to be good without any chipping, capping, and sticking. Various physical parameters like thickness, hardness, weight variation, friability, hardness, and disintegration time were measured to evaluate tablets. It was found that the average thickness of the tablets also ranged between 3.11 and 3.13 mm; however, the variations were not alarming and remained within the acceptable range. Hardness of tablets of the different formulations varied widely ranging from 3.0 to 3.4 kg/cm2. The loss in friability was ranged from 0.37 to 0.57% so all the postcompression parameters were in the limit and results are shown in Table 11 [3236].

3.3.1. Wetting Time

The wetting time of the tablets was measured using a simple procedure. Five circular tissue papers of 10 cm diameter were placed in a Petri dish containing 0.2% w/v solution of amaranth (10 mL). One tablet was carefully placed on the surface of the tissue paper. The time required to develop blue color due to amaranth water soluble dye on the upper surface of the tablets was noted as the wetting time [37].

3.3.2. Water Absorption Ratio

A small piece of tissue paper folded twice was placed in a small Petri dish containing 6 mL of water. A tablet was put on the paper for water absorption (Figure 5).

The wetted tablet was then weighed. Water absorption ratio, , was determined by using following formula:

Here, is the water absorption ratio, is the weight of tablet before water absorption, and is the weight of tablet after water absorption [38].

The water absorption ratio was found to be in range from 83.38 to 93.18%, whereas the wetting time of batch K1 to K6 was found from 19.49 to 28.32 and wetting time was significantly lower in K4 due to highly water absorption capacity (Table 12).

3.3.3. Drug Content

Ten tablets were powdered and 10 mg drug equivalent powder dispersed in phosphate buffer pH 6.8. Volume of the solution made up to 10 mL by media. The mixture was filtered and 1 mL of the filtrate was diluted to 10 mL using phosphate buffer pH 6.8. The absorbance of the sample preparations was measured at 231.0 nm for Levocetrizine dihydrochloride and 352.20 nm for Montelukast sodium.

Another method (Petri dish method) was used to calculate drug content in which 10 mL phosphate buffer pH 6.8 was taken in Petri dish and then a tablet was dipped in it and after 30 sec media was filtered (process repeated at least for 3 times) and the absorbance of the sample preparations was measured at 231.0 nm for Levocetrizine dihydrochloride and 352.20 nm for Montelukast sodium [39].

Uniformity of Drug Content. Ten tablets were selected randomly and average weight was calculated for both Levocetrizine dihydrochloride and Montelukast sodium. Tablets were crushed in a mortar and accurately weighed amount of drug was taken from the crushed blend. Then, the samples were transferred to 100 mL volumetric flasks and diluted up to the mark with methanol. The content was shaken periodically and kept for one hour to dissolve the drug completely. The mixtures were filtered and appropriate dilutions were made separately for both drugs. The drug content in each tablet was estimated at against blank reference and reported.

In the case of Levocetrizine dihydrochloride the drug content was found in the range of 94.65–98.74%, whereas 92.98–95.78% drug was found in case of Montelukast sodium (Table 13).

3.3.4. Disintegration Time

By Disintegration Test Apparatus. Disintegration time is considered to be one of the important criteria in selection the best formulation. To achieve correlation between disintegration times in vitro and in vivo, several methods were proposed, developed, and followed at their convenience [4043]. One tablet was placed into each tube and the assembly was suspended into the 1000 mL beaker containing phosphate buffer pH 6.8 maintained at 37°C. The apparatus was operated and time was taken as disintegration time when no particle of tablet remains on the mesh when it is at up position. The assembly was removed from the liquid and the tablets were observed.

Disintegration Time in the Oral Cavity. The healthy volunteers of either sex (age 18–25) were selected, trained, and then DT of each tablet for complete disintegration in the mouth was measured. The time when the tablet placed on the tongue disintegrated without leaving any lumps was taken as end point. After disintegration of tablet in the oral cavity the tablet contents were spit out and the oral cavity was rinsed with water. DT of six tablets per batch (three tablets per trained volunteer or total of two volunteers per batch) was recorded and the average was reported.

Disintegration Test Using Modified Dissolution Apparatus. Bi et al. suggested the use of a modified dissolution apparatus for disintegration (Figure 6), instead of the traditional disintegration apparatus. In this experiment, 900 mL of phosphate buffer (pH 6.8) was maintained at 37°C as the disintegration fluid and a paddle at 100 rpm as stirring element was used. Disintegration time was noted when the tablet disintegrated and passed completely through the screen of the sinker (3–3.5 mm in height and 3.5–4 mm in width, immersed at a depth of 8.5 cm from the top with the help of a hook). This method was useful in providing discrimination among batches which was not possible with the conventional disintegration apparatus (Figure 7).

Disintegration by Petri Dish Method. Petri dish method was used to calculate drug content in which 10 mL phosphate buffer pH 6.8 was taken in Petri dish then a tablet was dipped in it (Figure 8). Disintegration time was noted when the tablet disintegrated (process repeated at least for 3 times).

As per the pharmacopoeia requirement, formulation of fast disintegrating tablet should exhibit disintegration time in ≤60 seconds; K1 to K6 batches pass the disintegration time requirement. From the above it is observed that all the prepared formulations batches exhibited disintegration time less than 60 seconds and out of these K4 and K5 batch exhibited the least disintegration time (Table 14).

In all observations K4 and K5 were found suitable for further dissolution study as an optimized batch.

3.4. In Vitro Drug Release Study

K4 and K5 batch formulations were selected for drug release study. The Levocetrizine dihydrochloride and Montelukast sodium releases from different FDTs were evaluated by using the USP30 NF25 pharmacopoeia dissolution apparatus II—paddle at 37 ± 0.5°C using 900 mL of phosphate buffer (pH 6.8) as a dissolution medium with stirring speed of 50 rpm. Aliquots (5 mL) withdrawn at various time intervals were immediately filtered through Whatman filter paper, diluted suitably and analyzed at 231.0 nm for Levocetrizine dihydrochloride and 352.20 nm for Montelukast sodium [44].

The highest drug release was obtained with the formulation K4 containing super disintegrants SSG + CP in ratio of 2% (Levocetrizine dihydrochloride: Kyron-t-104 is 1 : 4). Hence, batch K4 was selected as optimized batch.

The results of dissolution are shown in Tables 15 and 16 and Figures 9 and 10.

3.5. Stability Studies of Optimized Batch

The optimized formulations (K4 and K5) were stored in aluminum capped clear glass vials and were subjected to a storage condition of 40°C ± 2°C/% RH for 3 months in humidity chamber. The samples were withdrawn at time intervals of 0, 1, 2, and 3 months and evaluated for hardness, friability, dispersion time, disintegration time, drug content, and in vitro dissolution study [45].

Stability study revealed (Tables 17, 18, and 19) that all the formulations were physically stable when stored at 40 ± 20°C and % RH till 3 months and there was no significant difference in dissolution for optimized formulation (Figures 11, 12, 13, and 14).

3.6. Determination of Similarity and Dissimilarity Factors

A model independent approach was used to estimate dissimilarity factor and a similarity factor to compare dissolution profile of optimized calculated FDTs with FDTs containing superdisintegrants. The FDA and SUPAC-IR guidelines define difference factor as the calculated percent (%) difference between the reference and test curves at each time point and are a measurement of the relative error between the two curves.

The similarity factor is given by the following equation:

The dissimilarity factor is given by the following equation: where is the number of pull points, is the reference batch profile at time point , and is the test batch profile at the same time . For in vitro dissolution curves to be considered similar values should be in the range of 0–15, while values of should lie within 50–100 [4649].

Similarity and dissimilarityfactors for K4 and K5 are shown in Tables 20 and 21. All formulations showed value between 50 and 100 and value below 15 indicating similar release profiles of the formulations before and after stability studies.

4. Conclusion

In the present work an attempt was made to use ion exchange resins (Kyron-T-104 and Tulsion-412) as taste masking agents for Levocetrizine dihydrochloride. Combinations of three superdisintegrants (separately and in ratio) were used in the formulation of fast disintegrating combination tablet of Levocetrizine dihydrochloride and Montelukast sodium. The purpose was to enhance patient compliance and provide fast onset of action. Kyron T-104 and Tulsion-412 were used as ion exchange resins and it was mixed with the drug in different ratios and evaluated for the extent of complexation. Results have shown that with Kyron T-104, drug to resin ratio of 1 : 4 and with Tulsion-412, drug to resin ratio of 1 : 5 gave maximum amount of drug loading. These drug-resin complexes further evaluated for taste masking and different conditions of drug loading and after optimization Kyron T-104 resinate with drug in ratio 1 : 4 selected for formulation development on the basis of maximum drug loading and cost effectiveness. All the blends (K1, K2, K3, K4, K5, and K6) for formulation exhibited satisfactory values for angle of repose, bulk density, Tapped density, Hausner ratio, and Carr’s index and shown good flow properties. All the tablets passed the weight variation test, friability test, hardness test, and % variation and were found within the pharmacopoeia limit. Drug content estimation showed that more than 90% of the drugs (Levocetrizine dihydrochloride and Montelukast sodium) was present. The dispersion produced was smooth with pleasant mouth feel and the bitter taste was totally masked in all formulations. The disintegration tests conducted on all these formulations showed that there was faster disintegration of the tablets, taking 16 to 31 seconds, which was much less than the official limit for fast disintegrating tablets (1 minutes). Minimum time for disintegration was shown by the formulations K4 and K5 so these two formulations finally selected for drug release study. In vitro drug release profile of tablet shown above 90% drugs (Levocetrizine dihydrochloride and Montelukast sodium) in 25–35 minutes in phosphate buffer (pH 6.8) indicating that the drug will be absorbed faster in the mouth, pharynx, and oesophagus and thus chances of enhancement the bioavailability by pregastric absorption through mouth, pharynx, and oesophagus. Stability study was conducted for 3 months. Similarity and dissimilarity factors for K4 and K5 were calculated. All formulations showed values between 50 and 100 and value below 15 indicating similar release profiles of the formulations before and after stability studies. There was no significant change in taste and color at optimized temperature. There was no significant variation in the disintegration time, hardness, friability, and in vitro dissolution profiles for the optimized formulations K4 and K5. On the basis of drug release K4 was the optimized formulation but as there was no significant difference between drug release profiles and other parameters of K4 and K5 and on the basis of cost effectiveness K5 may also be considered as optimized formulation.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.