Imiquimod Treatment Causes Systemic Disease in Mice Resembling Generalized Pustular Psoriasis in an IL-1 and IL-36 Dependent Manner
Generalized pustular psoriasis (GPP) is a severe form of psoriasis that can be caused by missense mutations in the interleukin-36 (IL-36) receptor antagonist. In addition to neutrophil rich skin inflammation, GPP patients typically also experience anorexia, fever, malaise, and pain. The imiquimod-induced skin inflammation mouse model has rapidly become a popular way to study plaque psoriasis, which typically does not involve symptoms of systemic disease. In this model, neutrophil recruitment to the skin is dependent upon the inflammatory mediators IL-1, via its receptor IL-1R1, and IL-36α. Unexpectedly, we observed that mice also exhibited signs of anorexia (weight loss and decreased food intake), general malaise (decreased activity and loss of interest in building nests), and pain (nose bulging and hunched posture). A scoring system allowing quantitative comparisons of test groups was developed. Female mice were found to develop more severe disease than male mice. Furthermore, mice deficient in both IL-1R1 and IL-36α are nearly disease-free, while mice lacking only one of these inflammatory mediators have less severe disease than wild type mice. Hence, the imiquimod-induced skin inflammation mouse model recapitulates not only plaque psoriasis, but also the more severe symptoms, that is, anorexia, malaise, and pain, seen in GPP.
Psoriasis represents a spectrum of inflammatory skin conditions ranging from mild to potentially life-threatening. Plaque psoriasis is the most common form and causes localized red scaly skin plaques. Generalized pustular psoriasis (GPP) is rare but is the most severe form of psoriasis and involves the formation of large sterile neutrophil pustules in the epidermis. The affected skin areas can cover the entire body and patients also suffer from anorexia, fever, malaise, and pain. Active disease flairs often appear abruptly and require hospitalization as the disease can be fatal. Progress towards developing a cure or satisfactory treatment approaches for GPP have been hampered by poor knowledge of disease mechanisms and the lack of experimental models in which drugs can be tested.
A major breakthrough in our understanding of GPP pathogenesis was made in 2011, when missense mutations in the interleukin-36 (IL-36) receptor antagonist, IL-36Ra, were identified in GPP patients [1, 2]. Several additional mutations have subsequently been identified (see  and the references therein). IL-36Ra is a classical receptor antagonist that acts as a natural inhibitor of the three related cytokines, IL-36α, IL-36β, and IL-36γ, by competitively binding to the common receptor IL-36R (see  and the references therein). The three IL-36 cytokines and IL-36R are related to the pleiotropic IL-1 cytokines, IL-1α and IL-1β, and their receptor, IL-1R1, respectively , and the IL-36 and IL-1 cytokines activate the same intracellular signaling mechanisms leading to, for example, production of cytokines such as CXCL1, CXCL2, and IL-8 by keratinocytes [1, 5–8]. This in turn promotes recruitment of neutrophils into the epidermis. Several GPP case studies, including some with IL-36Ra missense mutations, have reported successful treatment of both skin and systemic disease with granulocyte and monocyte adsorption apheresis, suggesting an important role for neutrophils in the disease (see [9, 10] and the references therein). Interestingly, some case studies have reported the efficacy of using IL-1 inhibitors, for example, the IL-1Ra or neutralizing antibodies, in GPP patients [11–14]. This suggests a role of both IL-36 and IL-1 in GPP pathogenesis.
The imiquimod-induced skin inflammation mouse model has been extensively used as a model of plaque psoriasis (reviewed in ). We have previously shown that IL-1 and IL-36 cooperate to promote neutrophil recruitment to the epidermis in this model [6, 7]. Here, we report the utilization of the imiquimod model to evaluate GPP related systemic disease such as anorexia, malaise, and pain. We also demonstrate dependence of these phenotypes upon IL-1 and IL-36, cytokines linked to GPP disease; hence, the model may be useful for mechanistic studies of GPP pathogenesis and drug development.
2. Materials and Methods
C57BL/6J and IL-1R1−/− mice were obtained from Jackson Laboratory. The IL-36α−/− and IL-36α−/−/IL-1R1−/− mice have previously been described by us . Wild type (C57BL/6J) and IL-1R1−/− mice for experiments involving IL-36α−/− and IL-36α−/−/IL-1R1−/− mice were bred in-house. All procedures were approved by the Temple University Institutional Animal Care and Use Committee.
2.2. Imiquimod Treatment
Mice aged 7–10 weeks were anesthetized using isoflurane and denuded by trimming and Nair treatment as previously described [6, 7]. Fur and Nair were gently and thoroughly removed using water and WypAll wipes (Kimberly-Clark Professionals) before returning the mice to their cages. Daily imiquimod treatments were started the following day. Mice were treated daily with 62.5 mg (or as indicated) 5% imiquimod cream on a 6 cm2 area on the lower back for a total of four applications. Aldara (imiquimod) cream was obtained from Valeant (NJ, produced by 3M Health Care Limited, UK). Generic imiquimod cream was from Perrigo (Israel). Mice were matched for age and sex in each individual experiment. Following fur removal, control mice were sham-treated with Vaseline cream (Riedel-de Haen) or left untreated.
2.3. Disease Evaluations
Mice were weighed daily. Body temperature was measured using noninvasive infrared thermometers, for example, the Forehead and Ear Thermometer (Innovo). To facilitate evaluation of nest building and food intake, mice were housed individually starting at day 0 (e.g., day of the first imiquimod application) throughout the remainder of the experiments. This short period of isolation did not significantly affect behavior in untreated mice. Mice were fed DietGel 76A (ClearH2O) during the experiments and the entire plastic container with food was weighed daily (replaced every two days). Mice were allowed access to water ad libitum. Nestlet (Ancare) material was replaced every day, for example, at the time of imiquimod treatment. Facial expressions of pain were evaluated as described by others . For total disease scores, changes in weight, food intake, and nest building were converted to scores. Each experiment, each including wild type controls, was conducted by the same person throughout the duration of the experiment to ensure consistent scoring. Mice in significant distress were euthanized.
2.4. Statistical Analyses
Each experiment contained four mice per test group and all experiments were performed at least 3 independent times. Statistical analyses were performed using paired and unpaired -tests as appropriate. Differences in survival were evaluated using Mantel-Cox and Gehan-Breslow-Wilcoxon tests.
3.1. Generic Imiquimod Cream Causes More Severe Disease Than Aldara Cream
Previously, using the imiquimod-induced skin inflammation mouse model, we demonstrated that IL-1 and IL-36α cooperate to promote epidermal neutrophil recruitment . Aiming to extend upon these studies, we serendipitously found that a generic version of imiquimod cream (Perrigo) caused significantly more severe phenotypes in terms of weight loss and survival than the brand name Aldara imiquimod cream (Figure 1).
3.2. Systemic Disease Can Be Scored for Anorexia, General Malaise, and Pain
During our earlier studies, we frequently observed behavioral changes, for example, reduced interest in nest building, in especially our wild type mice suggesting that the imiquimod treatments had effects beyond induction of skin inflammation. Infections and injuries can be associated with systemic responses such as anorexia, fever, general malaise, and muscle and joint pain as part of the body’s efforts to reestablish homeostasis . These physiological responses resemble some of the clinical manifestations reported in GPP patients; hence, to better characterize our behavioral observations and develop an approach allowing quantification of systemic disease responses, we established a scoring system to measure clinical manifestations often observed in GPP, that is, anorexia, general malaise, and pain (Figure 2 and Table 1). Malaise was evaluated by observing the physical activity of the mouse itself (Figures 2(a) and 2(b) and Table 1) and its progress in building a nest overnight (Figures 2(c) and 2(d) and Table 1). Pain was detected through posture (Figures 2(e) and 2(f) and Table 1) and facial expressions such as nose bulging (Figures 2(g) and 2(h) and Table 1) and orbital tightening (Figures 2(i) and 2(j) and Table 1). The use of facial expressions is based on a recent report correlating this to pain . We found the nose bulge to be a common and easy-to-score outcome (Figures 2(g) and 2(h)). Orbital tightening scoring as 2 (Figure 2(i), imiquimod) was only observed in very sick animals and was an indicator of euthanasia being required. Due to weight loss, the swim test was not applied as a measure of depression/malaise. Similarly, as the back skin can become stiff in response to the imiquimod cream, the tail-suspension test was not used. To evaluate anorexia, we determined food intake (Figures 2(k) and 2(l) and Table 1) and body weight (Figure 2(m) and Table 1). Progressive changes in these disease parameters were observed in response to repeated imiquimod treatments over a 4-day period (Figure 2). Sums of individual disease parameters reflected overall disease progression (Figure 2(n)). Based on a single controlled experiment involving two groups treated, respectively, in the morning and the late afternoon, timing of the imiquimod cream applications did not affect disease outcomes (data not shown). Furthermore, we did not detect differences in temperature or between control mice that were either sham-treated with Vaseline cream or left untreated (data not shown).
3.3. Female Mice Develop More Severe Disease Than Male Mice
In some initial experiments using generic imiquimod cream and female mice, mice either died unexpectedly or had to be euthanized before the end of the experiments due to significant distress and dramatic weight loss (data not shown and data below). To compare male and female mice, we used a reduced dose of generic imiquimod cream. As expected based on the initial experiments, female mice exhibited several signs of more severe systemic disease than male mice (Figure 3). While the readouts for activity (malaise) and posture (pain) only revealed trends towards gender specific differences, nest building (malaise), weight (anorexia), and nose bulging (pain) were significantly more affected in female than in male mice (Figure 3). To the best of our knowledge, this is the first time sex specific differences in the imiquimod model are reported; however, such gender specific differences are in general agreement with the long established stronger immune responses observed in females .
It should be noted that we here used a fixed dose and not a treatment adjusted to the size of the individual mouse. This approach is in agreement with that employed when skin inflammation induced by imiquimod cream is examined (see  for references). Since female mice are smaller than male mice, the females were treated with a greater dose per gram body weight. Hence, it cannot be concluded whether female mice per se are more sensitive to the drug than male mice.
3.4. Systemic Disease Is IL-1R1 and IL-36α Dependent
We previously found that both IL-1R1 and IL-36α are important for the development of psoriasis-like skin inflammation in the imiquimod model [6, 7]; thus, we next examined their role in systemic disease (Figure 4). No differences between sham treated wild type, IL-1R1 knockout, IL-36α knockout, and IL-1R1/IL-36α double knockout mice were detected (data not shown). Mice lacking IL-1R1 (Figure 4, blue bars, lines, and symbols) exhibited significantly less overall disease due to diminished signs of malaise, pain, and anorexia compared to wild type mice (Figure 4, open bars and black lines and symbols) when treated with imiquimod. This is in agreement with the known role of IL-1β in inducing fever and pain [19, 20]. Interestingly, mice deficient in IL-36α also experienced milder disease (Figure 4, red bars, lines, and symbols). This effect was most pronounced in the IL-1R1/IL-36α double knockout mice, which exhibited largely normal behavior and no signs of pain (Figure 4, purple bars, lines, and symbols). Hence, the systemic disease induced by imiquimod in mice is dependent upon inflammatory mediators known to play a role in human GPP.
The imiquimod-induced skin inflammation mouse model is frequently used to study mechanisms of psoriasis pathogenesis and for testing new drugs. GPP is a severe form of psoriasis, which is also associated with anorexia, fever, malaise, and pain. Here, using the imiquimod model, we demonstrate that mice develop symptoms of systemic disease (Figures 1 and 2) resembling those observed in GPP, that is, anorexia, malaise, and pain. Furthermore, we have developed a scoring system (Table 1) that allows quantitative comparisons between test groups (Figures 3 and 4). The IL-1/IL-36 dependence of the systemic phenotypes in this mouse model (Figure 4) suggests strong correlation to human GPP pathogenesis, as IL-36Ra missense mutations are now known to cause GPP (see [1–3] and the references therein) and at least some GPP patients experience dramatic improvement of their disease when treated with IL-1 inhibitors [11–14].
While the observed phenotypes are largely IL-1 and IL-36α dependent (Figure 4), mild anorexia and weight loss were still observed in IL-1R1/IL-36α double knockout mice (Figures 4(f) and 4(g)). This suggests additional mechanisms contributing to these disease outcomes. Interestingly, onset of anorexia and weight loss is very rapid, that is, apparent already on day 1 (Figures 2(k)–2(m)), and disease severity improves during the experiment (days 3-4, Figures 2(k)–2(m)). Hence, the IL-1R1/IL-36α independent effect upon food intake and body weight appears to involve a transient induction/response and/or negative regulator. The identification of this mechanism(s) will require further examination.
It has been reported that isostearic acid in Aldara contributes to the inflammatory response induced by the cream . Interestingly, the generic cream produced by Perrigo does not contain isostearic acid; hence, this compound cannot explain why the generic form of the drug causes more severe systemic disease than the brand name cream (Figure 1). However, the Perrigo cream contains oleic acid, which is a common and naturally occurring fatty acid in, for example, adipose tissue and olive oil. While oleic acid does not itself promote inflammation, it has been reported that it can enhance (2–4-fold) IL-23 production by dendritic cells in response to the inflammatory mediator LPS . The role of the IL-23/IL-17 axis and cross-communication between dendritic and epithelial cells in the imiquimod model in an IL-36 receptor dependent manner has been reported [23, 24]. Whether the Perrigo cream contains sufficient oleic acid to potentiate the in vivo inflammatory response of dendritic cells to imiquimod in the model remains to be determined.
While oleic acid in generic imiquimod cream may explain why this has higher activity than Aldara (Figure 1), imiquimod appears to be the primary driver of the systemic phenotypes as these can be observed with Aldara, generic imiquimod cream, and soluble imiquimod. While this manuscript was in preparation, an independent study reported that imiquimod (Aldara or soluble imiquimod) applied specifically to the skin decreased burrowing activity , which involves searching for hidden food. This outcome may be a consequence of malaise (activity and nest building) and anorexia (food intake) as reported here, for example, Figure 2. The pain experienced by the mice may be an additional contributing factor. Since burrowing is no longer a human activity, the here used readouts (Table 1 and Figure 2) may be more useful, when imiquimod is used to model human disease outcomes, including anorexia, malaise, and pain as seen in GPP patients.
The reported decreased burrowing activity in response to topical imiquimod was associated with gene expression changes and inflammation in the brain . The IL-36 system of cytokines and receptor is expressed in the brain [26–29]; however, early studies seeking to identify IL-36 functions failed at detecting effects of recombinant proteins both in vivo and in vitro [26, 27]. In light of the recent discovery that IL-36 activity is enhanced by removal on N-terminal amino acids from the recombinant IL-36 proteins , it is possible that the shorter IL-36 cytokines could drive neuroinflammation . Lack of processing of IL-36γ in experimental autoimmune encephalomyelitis has been used to explain why IL-36γ and IL-36R KO mice develop the same degree of disease as wild type mice, despite high levels of the cytokine being expressed in the brain during disease . Given the observed effect of IL-36α deletion in the outcome of imiquimod treatment (Figure 4), it is possible that in this model system IL-36α acts directly on the brain. Further studies will be required to determine exactly how IL-36α promotes systemic disease.
In summary, treatment of mice with imiquimod cream causes systemic phenotypes resembling those observed during infections and in patients with GPP. The imiquimod-induced skin inflammation mouse model with behavioral observations, as described here, may represent a suitable model in which novel therapies can be developed to manage GPP. Since IL-1 and IL-36 are known to be involved in the human GPP condition, the model may also be valuable to studies aimed at elucidating disease mechanisms.
|GPP:||Generalized pustular psoriasis|
The current address of Pilar Alvarez is the Department of Molecular and Cellular signalling, Instituto de Biomedicina y Biotecnología de Cantabria, CSIC-Universidad de Cantabria-SODERCAN, PCTCAN, Cl. Albert Einstein 22, 39011 Santander, Spain.
The authors declare that there are no competing interests regarding the publication of this paper.
This study was supported in part by an award (AI125111) from the National Institute of Allergy and Infectious Diseases to Liselotte E. Jensen. Pilar Alvarez was supported by Ayudas a la Movilidad Predoctoral para la Realización de Estancias Breves en Centros de I+D 2015 (EEBB-I-16-11190).
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