Impact of Highly Saturated versus Unsaturated Fat Intake on Carbohydrate Metabolism and Vascular Reactivity in Rat

Djohan, Youzan Ferdinand; Raynaud, Fabrice; Lambert, Karen; Cristol, Jean-Paul; Coudray, Charles; Feillet-Coudray, Christine; Virsolvy, Anne; Badia, Eric

doi:https://doi.org/10.1155/2022/8753356

Biochemistry Research International

On this page

Abstract Introduction Materials and Methods Results Discussion Abbreviations Data Availability Ethical Approval Conflicts of Interest Authors’ Contributions Acknowledgments Supplementary Materials References Copyright Related Articles

Research Article | Open Access

Volume 2022 | Article ID 8753356 | https://doi.org/10.1155/2022/8753356

Impact of Highly Saturated versus Unsaturated Fat Intake on Carbohydrate Metabolism and Vascular Reactivity in Rat

Youzan Ferdinand Djohan,¹Fabrice Raynaud,²Karen Lambert,²Jean-Paul Cristol,³Charles Coudray,⁴Christine Feillet-Coudray,⁴Anne Virsolvy,²and Eric Badia²

Academic Editor: Néstor Gutiérrez-Méndez

Received17 May 2022

Accepted26 Jul 2022

Published19 Aug 2022

Abstract

Palm olein (PO) and lard are considered harmful to health because of their highly saturated fatty acid content. On the contrary, olive oil (OO) with its high level of polyunsaturated fatty acids is considered healthier. This study aims to evaluate the effects of high consumption of these oils on carbohydrate metabolism and vascular function. Male Wistar rats were fed ad libitum for 12 weeks with different high fat diets (HFD) containing 30% of each oil. Systemic glycemia, insulinemia, and lipidemia were assessed by routine methods or by ELISA. GLUT4 muscular expression and hepatic and muscular Akt phosphorylation were analyzed by western blot. Vascular function was evaluated, ex vivo, on aortic rings and on the variations of isometric tensions. The results show that fasting blood glucose was increased with PO and OO diets and decreased with lard. Compared to control diet, this increase was significant only with PO diet. The area under the curve of IPGTT was increased in all HFD groups. Compared to control diet, this increase was significant only with PO. In contrast, stimulation of the pathway with insulin showed a significant decrease in Akt phosphorylation in all HFD compared to control diet. KCl and phenylephrine induced strong, dose-dependent vasoconstriction of rat aortas in all groups, but KCl EC₅₀ values were increased with lard and OO diets. The inhibitory effect of tempol was absent in PO and lard and attenuated in OO. Vascular insulin sensitivity was decreased in all HFD groups. This decreased sensitivity of insulin was more important with PO and lard when compared to OO diet. In conclusion, the results of this study clearly show that high consumption of palm olein, olive oil, and lard can compromise glucose tolerance and thus insulin sensitivity. Furthermore, palm olein and lard have a more deleterious effect than olive oil on the contractile function of the aorta. Excessive consumption of saturated or unsaturated fatty acids is harmful to health, regardless of their vegetable or animal origin.

1. Introduction

A high-fat diet (HFD) over time promotes metabolic syndrome. The metabolic syndrome consists of many health problems, such as glucose intolerance, obesity, dyslipidemia, insulin resistance, and high blood pressure [1–4]. All these disturbances observed in metabolic syndrome increase the risk of the occurrence of cardiovascular diseases and type 2 diabetes [5]. Saturated fatty acids (SFA) are thought to be involved in the development of type 2 diabetes [6–8] and cardiovascular diseases [9, 10]. Monounsaturated fatty acids (MUFA) or polyunsaturated fatty acids (PUFA) are said to be beneficial to health [11–13]. The general consensus is that the dietary intake of SFA increases cardiovascular risk but that MUFA and PUFA intake decreases risk [10].

Cardiovascular diseases are caused by numerous changes in the arterial vasculature such as arterial stiffening [14], arterial wall thickening, and endothelial dysfunction [15]. To maintain the integrity of the artery in the presence of stimuli, the components of the arterial wall reorganize with the risk of increasing the potential for vascular dysfunction [16].

Palm olein (PO) and lard are rich in saturated fatty acids with similar compositions. Palm olein is composed of 50% SFA, 40% MUFA, and 10% PUFA, [17]. Lard is composed of 41% SFA, 47% MUFA, and 12% PUFA [18]. Olive oil (OO) is composed of 14% SFA, 77% MUFA, and 9% PUFA [19]. OO contains less saturated fatty acids and more monounsaturated fatty acids than PO or lard. The latter are considered to be harmful to health because of their composition in SFA. They also contain fewer compounds (vitamin E for PO and gallate for lard) with antioxidant properties [20, 21] known to attenuate the deleterious effect of SFA.

The aim of the present study was to evaluate the effects of long-term consumption of a hypercaloric diet high in saturated fatty acids versus unsaturated fatty acids on carbohydrate metabolism and arterial vessels’ function. Thus, we compared the effects of HFD rich either in palm olein, lard, or olive oil on insulin resistance and vascular reactivity in rats.

2. Materials and Methods

2.1. Animals and Diets

The experiments complied with the guidelines for the care and use of laboratory animals (National Academies Press US, 8^th edition, 2011), and all procedures were approved by the local ethical committee (reference CEEA-LR-12002, Montpellier, France). Thirty-two young male Wistar rats aged 6 weeks coming from the Animal facilities of Charles River Laboratories, France, were used. The animals were housed under conditions of constant temperature (20–22°C), humidity (45–50%), and a standard dark cycle (20.00-08.00 h) with free access to food and water. Rats were randomly assigned to four groups of eight animals and fed for 12 weeks either a standard rat chow diet (control) or one of the three HFD. In the control diet, 11% of the energy was given by fat (5% soybean oil), whereas in enriched diets, 56% of the energy was provided by fat intake [22]. The fat-enriched diets consisted in 2.5% (w/w) of soybean oil and 30% (w/w) of PO (Sania Cie, Abidjan, Côte d’Ivoire), OO (virgin olive oil, bought in a supermarket), or lard (Alva, Rezé, France). The detailed composition of these experimental diets is given in Table 1.

2.2. Intraperitoneal Glucose Tolerance Test

The intraperitoneal glucose tolerance test (IPGTT) was completed seven days before rat sacrifice and performed as previously described [23, 24]. Briefly, after being fasting for 16 h, a glucose solution [2 g/kg body weight saline (0.9% NaCl)] was administered intraperitoneally. Blood was sampled through the tail vein of conscious rats immediately prior to the injection, and 20, 30, 40, 60, 90, and 120 min afterward. Blood glucose was measured using glucose strips and a commercial glucometer (AccuChek Active, Roche Diagnostics, USA). The area under the curve (AUC) was calculated by the trapezium method. The AUC values are expressed as glucose per L per 120 min.

2.3. Rat Sacrifice and Sampling

Rats were fasted overnight, and blood was sampled via the abdominal artery under 1% pentobarbital anaesthesia (50 mg/kg ip). After centrifugation at for 10 min at 4°C, plasma was collected and stored at −80°C until analysis. Rat gastrocnemius was quickly removed; one piece was immediately frozen in liquid nitrogen and then kept at −80°C until analysis. The left soleus and right soleus were immersed, respectively, in a 0.7 μM insulin solution or PBS without insulin at 37°C for 15 minutes. The two solei were then rinsed and frozen in liquid nitrogen.

2.4. Routine Biochemical Analyses

The plasma contents of glucose, total cholesterol, HDL cholesterol, and triglycerides, were determined using standard methods on a COBAS automated analyzer (Roche Diagnostics, France). Plasma insulin level was quantified by an immunoassay kit (Mercodia Rat Insulin ELISA). The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated using the follow equation: HOMA-IR = [fasting blood glucose level (mmol·L⁻¹) × fasting plasma insulin level (mUI·L⁻¹)]/22.5.

2.5. Protein Isolation and Western Blot Analysis

Protein isolation and western blot analysis were performed as previously described [25]. Whole-cell protein lysates from gastrocnemius and soleus were prepared in the following lysis buffer: 20 mM Tris pH 8, 50 mM DL-dithiothreitol, 2 mM EDTA, 150 mM NaCl, 0.1% SDS, 1% Triton X100, 1 mM PMSF, 1 mM orthovanadate, and 1% (v/v) of antiprotease cocktail (Sigma-Aldrich, Saint-Quentin-Fallavier, France). Proteins were resolved by SDS-PAGE and then transferred to nitrocellulose membranes at 20 V overnight using refrigerated Tris-glycine transfer buffer. The membranes were blocked in 5% nonfat milk in PBS (without Tween) for 1 h at room temperature. Then, membranes were incubated overnight with primary antibody of protein kinase B (Akt), phospho-protein kinase B (p-Akt) (Cell Signaling, Leiden, The Netherlands); glucose transporter Glut 4 (GLUT 4) (Abcam, Cambridge, UK); tubulin (Sigma-Aldrich, Saint-Quentin-Fallavier, France) in blocking buffer. After washes in PBS/Tween under gentle agitation, the membranes were incubated for 45 min in the dark with the fluorescent-labeled secondary antibodies and finally quantified using the Odyssey infrared imaging system (LI-COR, Lincoln, USA).

2.6. Vascular Reactivity

The thoracic aorta was used to study ex vivo the responses to agonists and antagonists of arterial contraction as previously described [26]. Immediately after removal, arterial tissue was immersed in phosphate saline solution (PSS), pH 7.4, containing (in mM) 140 NaCl, 5 KCl, 1 MgCl₂, 0.5 KH₂PO₄, 0.5 Na₂HPO₄, 2.5 CaCl₂, 10 HEPES, and 10 glucose. Aortic tissue was cleaned of fat and connective tissue and cut into 2-3 mm wide rings. Aortic rings were mounted in standard organ bath chambers (EMKA Technologies, Paris, France) maintained at 37°C and continuously bubbled with O₂. Then, the changes in isometric tension were recorded according to Fort et al. [27]. Each arterial segment was subjected to a 60 min equilibration period at the predetermined optimal basal tension of 2 g. The contractile function of each segment was assessed with 1 µM phenylephrine (Phe), and the presence of endothelium was confirmed by the vasorelaxation induced by application of acetylcholine (Ach, 1 µM). After several washouts and a 20–30 min period of stabilization, dose responses were performed by cumulative increases in the concentration of the agonist Phe (0.01–100 µM range) or the depolarizing agent KCl (1–80 mM). Endothelial function was assessed by studying the relaxing effects of cumulative increases of Ach between 1 nM and 10 µM on arteries contracted with a submaximally active concentration of Phe (10 µM). The effects of the nitric oxide (NO) synthase inhibitor nitro-L-arginine methyl ester (L-NAME, 10 µM) and the reactive oxygen species scavenger tempol (100 µM) were evaluated on the contractile response to Phe and the relaxing effect of Ach. Inhibitors were added for a 15 min period of incubation before Phe addition. Endothelium-independent relaxations to sodium nitroprusside (SNP, 1 nM–200 µM) were studied in endothelium-denuded rings previously contracted with Phe (10 µM). Each protocol was performed in triplicate for each aorta. All chemical compounds were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).

2.7. Statistical Analysis

Values were expressed as mean ± SD, n = 7-8 animals per group. Statistical analysis was based on one-way ANOVA followed by a Tukey Kramer multiple comparisons test. When statistical variances were unequal, a Welch test was performed. Dose-response data were analyzed and compared by nonlinear regression methods using the PRISM software (GraphPad, San Diego, CA). Differences between dose–response curves were investigated by the half maximum effective concentration or sensitivity (EC50) and maximum effect (E_max). The limit of statistical significance was set at . The group mean values with different letters (a, b, c, d) are significantly different. Statistical analyses were performed using the Stat-View program (SAS Institute, Cary, NC, USA).

3. Results

3.1. Effect of Diets on Body Weight, Lipid Parameters, and Antioxidant Defenses

As shown in Table 2, body weight was significantly increased () by HFD (+15%) whatever the type of fat and with no difference between diets and type of fat. No increase in plasma total cholesterol and triglyceride levels was observed. Even in OO and lard groups, plasma levels of total cholesterol () and HDL cholesterol () were decreased. Compared to control diet, plasma HDL cholesterol was decreased with OO (−10.54%) and lard (−28.88%) diets and slightly increased with PO diet (+4.49%). Only lard significantly decreased HDL cholesterol compared to control diet. Blood SOD activity was decreased with PO and lard diets when compared to control and OO diets (Table 2).

3.2. Effect of Diets on Glucose Tolerance and Insulin Resistance

Fasting blood glucose was increased with PO (+13%) and OO (+8%) diets and decreased with lard (−5.43%). When compared to control diet, this increase was significant with PO () but not OO diet. With lard diet, glycemia was unchanged. Although no diet significantly changed fasting insulin levels and HOMA-IR, all HFD increased the value of these two parameters compared to control diet. HOMA-IR index was, however, higher with PO diet (Table 2).

Whole body glucose tolerance was investigated with intraperitoneal glucose injection (IPTG) (Figure 1). No significant differences were observed between groups at T0, T20, T30, T90, and T120 minutes. However, compared to the control diet, with OO and PO diets, blood glucose at T40 () and T60 minutes () was significantly higher (Figure 1(a)). The area under the curve (AUC) of IPGTT was increased in all HFD groups (PO: +20.96%, OO: +13.44%, lard: +8.60%). Compared to control diet, this increase was significant only with PO (), and no difference was observed between the different HFD (Figure 1(b)). These results evidenced a decrease in glucose handling with diets and especially PO.

(a)

(b)

Since skeletal muscles are major regulators of insulin-stimulated glucose uptake, we investigated muscle insulin resistance with the p-Akt/Akt ratio. Insulin signaling pathway was evaluated in soleus by the level of Akt phosphorylation. No significant difference was observed between groups in basal Akt activation as assessed by the p-Akt/Akt ratio (Figure 2). In contrast, stimulation of the pathway with insulin showed a significant decrease () in Akt phosphorylation in all HFD compared to control diet. However, no differences between HFD themselves were found (Figure 2). Together with the trend toward increased insulin level and HOMA-IR in HFD groups, these results are in favor of a decreased insulin sensitivity at the whole body and muscle level. Muscle expression of the glucose transporter GLUT4 was not affected by the diets (Supplemental Figure 1).

(a)

(b)

(c)

Figure 2

Effects of diets on phospho-Akt protein expression in muscle (soleus) in the absence or presence of insulin. Representative western blot of either total Akt or phospho-Akt with tubulin as a normalizing gene (a). The histograms of blot quantification in the absence of insulin (b). The histograms of blot quantification in the presence of insulin (c). Results were expressed as mean values ± SD, n = 7-8 animals per group. The limit of statistical significance was set at . The group mean values with different letters (a, b, c) are significantly different. Akt or PKB: protein kinase B; p-Akt: phosphorylated Akt; NS: not significant.

3.3. Effect of Diets on Vascular Function

Contractile properties were evaluated on aortic rings with the depolarizing agent KCl and with the α-adrenergic agonist phenylephrine (Phe). Both agonists induced strong, dose-dependent vasoconstriction of rat aortas in all groups (Figure 3). Concerning KCl-induced responses, no significant difference was observed between groups on Emax values (Figure 3(a)), but EC50 values were increased with lard (+32%) and OO (+13%) diets. This increase was significant () with lard diet compared to control diet (Table 3). This change, reflected by a rightward shift of the dose-response curves, traduced an increased sensitivity to depolarization consecutive to aortic tissue hyperpolarization.

(a)

(b)

Figure 3

Contractile responses of rat aorta. Graphs represent cumulative response curves (a) to KCl (1–80 mM) and (b) to phenylephrine (0.01–100 μM). Histograms represent the maximal contraction (E_max) induced by a maximally active concentration of KCl (a) and phenylephrine (b). Results were expressed as mean values ± SD, n = 7-8 animals per group. The limit of statistical significance was set at . The group mean values with different letters (a, b, c) are significantly different. KCl: potassium chloride.

The contractile responses to Phe (0.01–100 μM) were increased in the aortas of rats fed with PO and lard, while no difference was observed in the OO group compared to the control group (Figure 3(b)). EC50 values, illustrating Phe sensitivity, were not modified by the diets (Table 3).

To determine whether contractile response alterations were due to changes in basal NO contribution, we analyzed the differences in Phe response curves performed in the presence of L-NAME (Figure 4) and of tempol (Figure 5). Preincubation with L-NAME, a nitrate synthase inhibitor, enhanced contractile responses to Phe in all groups. This increase is due to inhibition of NO production and suppression of its subsequent vasorelaxant tone. This effect was markedly more important in HFD groups () and especially with OO diet when compared to control group (Figure 4(a)) as depicted by area under curve analysis (Figure 4(b)).

(a)

(b)

Figure 4

Effect of L-NAME on the contractile response to Phe. (a) Concentration-response curves to phenylephrine (0.01–100 µM) in aortic rings from control, olive oil, palm olein, and lard diets in absence and in presence of L-NAME (10 µmol/L). (b) Histogram represents the difference in area under curve between contractions with and without L-NAME for each group expressed as percentage of Phe effect. Results were expressed as mean values ± SD, n = 7-8 animals per group. The limit of statistical significance was set at . Histogram bars with different letters (a, b, c) are significantly different. AUC: area under the curve; L-NAME: nitro-L-arginine methyl ester.

(a)

(b)

Figure 5

Effect of tempol on the contractile response to Phe. (a) Concentration–response curves to phenylephrine (0.01–100 µM) in aortic rings from control, olive oil, palm olein and lard diets in absence and in presence of tempol (100 µmol/L). (b) Histogram represents the difference in area under curve between contractions with and without tempol for each group expressed as percentage of Phe effect. Results were expressed as mean values ± SD, n = 7-8 animals per group. The limit of statistical significance was set at . Histogram bars with different letters (a, b, c) are significantly different. AUC: area under the curve.

Conversely, incubation with tempol, a SOD mimetic and ROS scavenger, decreased the contractile response to Phe (Figure 5(a)) due to O₂⁻ dismutation and suppression of O₂⁻-induced contractile tone. This inhibitory effect of tempol was absent in PO and lard and attenuated in OO as depicted by AUC analysis (Figure 5(b)).

Relaxant properties were evaluated with the antagonists Ach and SNP to, respectively, investigate endothelium-dependent and endothelium-independent relaxation. Ach-induced dose-dependent relaxation of previously contracted arterial rings reflected endothelial function (Figure 6(a)). SNP, a NO donor, also induced dose-dependent relaxation of arterial rings that involved smooth muscle cells (Figure 6(b)). In the different groups, we observed no modification of either endothelial function based on acetylcholine-induced endothelium-dependent relaxation (0.01–100 µM) (Figure 6(a)) or SNP-induced endothelium-independent relaxation (0.01–200 µM) (Figure 6(b)). No significant difference between control and OO, PO, and lard diets either for the E_max and the EC₅₀ values for both Ach and SNP was detected (Figure 6 and Table 3).

(a)

(b)

(c)

Figure 6

Effects of diets on relaxation of phenylephrine-precontracted aortic rings. Endothelium-dependent relaxation induced by acetylcholine (a) and endothelium-independent relaxation induced by SNP (b) and relaxant effect of insulin (c). Graphs represent cumulative response curves to acetylcholine (0.01–10 µM) in the absence and in the presence of L-NAME (10 µM), SNP (0.1 nM–1 µM) and Insulin (0.1–50 µM) on aortic rings previously contracted with 10 µM Phe. Histograms represent the maximal relaxation obtained for Ach and SNP and the relaxation induced by 20 µM insulin. Results were expressed as mean values ± SD, n = 7-8 animals per group. The limit of statistical significance was set at . The group mean values with different letters (a, b, c) are significantly different. Ach: acetylcholine; L-NAME: nitro-L-arginine methyl ester; SNP: sodium nitroprusside.

In the presence of L-NAME, Ach-induced relaxation was strongly inhibited (Figure 6(a)) in all groups. No difference was observed between groups for L-NAME effect. In the presence of tempol, Ach-induced relaxation was slightly increased in all groups with no difference observed between groups (not shown).

As insulin resistance is characterized by the inability of insulin to induce proper signal transduction leading to impaired insulin-induced vasodilation, we studied the vasorelaxant properties of insulin on Phe-contracted aortic rings. We observed that insulin induced a dose-dependent vasorelaxant response in aortic rings for all groups (Figure 6(c)). Vascular insulin sensitivity was decreased in all HFD groups when compared to control as shown by the relaxant response to 20 µM insulin (bar graph in Figure 6(c)). This decreased sensitivity of insulin was more important with PO and lard when compared to OO diet.

4. Discussion

Although numerous studies have shown vascular dysfunction in rat on HFD, no studies to date have compared the effects of saturated fatty acids versus unsaturated fatty acids in diets. In the present study, we show that any excess of fat, whatever its origin (vegetable or animal) and its nature (saturated or unsaturated) induced weight gain is, disrupted lipidemic status and carbohydrate metabolism and decreased insulin sensitivity. However, only diets enriched with saturated fatty acids (PO and lard) increased arterial procontractile tone involving reduced NO bioavailability and increased oxidative stress.

The disruption of carbohydrate metabolism, highlighted by many authors [28–33], was marked by muscle insulin resistance induced by significant decrease in Akt activation [34, 35], which promoted the increase in insulin levels [36] and HOMA-IR with all HFD. Otherwise, it has been observed that high OO diet over a long period of time induces obesity and insulin resistance similar to what is observed with lard-based diet [37]. Our results showed that all HFD promoted insulin resistance, which was more important with PO. Decreased vascular insulin sensitivity was also observed with PO and with lard diets. In our model, PO seems more diabetogenic than lard and OO. Moreover, the lipid status is modified even if it is not strongly altered. HDL-C was reduced with lard and less significantly with OO. According to many authors [38, 39], the decrease of HDL-C is one of the major risk factors for the occurrence of cardiovascular diseases (CVD). The role of SFA in CVD is related to their carbon number and position on the triglycerides [40, 41]. In fact, cardiovascular risk factors (Total Cholesterol, LDL-C, HDL-C, and VLDL-C) are more important when the main SFA in the diet are short-chain FAs: lauric acid (C12:0) and myristic acid (C14:0) [41, 42]. The atherogenicity of a diet is related to the degree of saturation of the fatty acids located in position sn-2 of the triglycerides [43, 44]. In PO, 85% of the fatty acids in the sn-2 position are MUFA or PUFA [43, 45], whereas in lard, 61% of the fatty acids in the sn-2 position are SFAs, notably palmitic acid [46]. This arrangement of fatty acids on triglycerides could explain the normal profile induced by PO and the atherogenic profile induced by lard compared to control diet.

Many studies have shown that high fat diets contribute to vascular dysfunction. In animal models, abnormalities in vascular reactivity consist primarily of increased contractility and reduced vasodilation associated to endothelial dysfunction [47]. Endothelial function can be reflected by acetylcholine-induced relaxation [26]. In this study, we observed that none of the diets disrupted acetylcholine-induced relaxation, showing that PO, OO, and lard diets did not elicit endothelial dysfunction in our model. In contrast, PO and lard HFD promoted a contractile dysfunction revealed by an increase in the maximal Phe-induced contraction. The fact that the diets in the study did not promote endothelial dysfunction could be explained, on the one hand, by their antioxidant content. OO and PO contain several classes of minor components such as polyphenols, carotenoids, and vitamin E, which have antioxidant properties [48–50]. Lard on the market is enriched with gallate, which is a powerful antioxidant [51]. Indeed, many studies [52–54] have highlighted the role of antioxidants in improving endothelial function.

On the other hand, whether endothelial dysfunction is a common feature of HFD-induced vascular alterations, numerous studies report no difference in Ach-induced endothelium-dependent relaxation of arteries [55], and early endothelial dysfunction may be masked by compensatory mechanisms. Interestingly, it has been shown in some models that insulin-induced vasodilation, which is also endothelium-dependent, is impaired earlier than dilation to acetylcholine [56]. In our study, we evidenced decreased vascular insulin sensitivity with HFD and especially with PO and lard. Such mechanisms could occur in our model. Enhanced vasoconstriction, as a result of enhanced oxidative stress and ROS production, has been documented in insulin resistance [57].

The endothelium constitutively produces NO and hyperpolarizing factors, which participate in baseline tone through anticontractile activity [58]. NO signaling depends on a delicate balance between NO production via endothelial NO synthase and its inactivation by ROS such as superoxide [59]. In the presence of L-NAME, which is an inhibitor of NO synthase, the vasorelaxant tone of NO is suppressed, and the response to Phe is increased. We observed that the effect of L-NAME on Phe-induced response is potentiated in all HFD groups suggesting that NO or NO metabolites participation in baseline tone was increased. This could reflect an alteration in either NO production or signaling. As no endothelial dysfunction was evidenced in our model, altered NO production is ruled out in favor of impaired NO signaling and bioavailability. NO scavenging by superoxide anion (O₂⁻) could be increased leading to increased peroxynitrite production and hypercontractility. Consistent with this hypothesis and with a previous study [60], on the one hand, we observed a decreased SOD activity with PO and lard diet. On the other hand, the effect of tempol on Phe-induced contractile response was significantly decreased with PO and lard diets, indicating that O₂⁻ is not the main ROS involved in vascular tone.

Taken together, our results demonstrated that all HFD altered vascular function and NO availability rather than NO production. With PO and lard diets, a vascular procontractile dysfunction associated to increased oxidative stress and NO oxidation is observed, while, in comparison with OO diet, NO oxidation is reduced in favor O₂⁻ dismutation.

In conclusion, the results of this study clearly show that high consumption of PO, OO, and lard can compromise glucose tolerance and thus insulin sensitivity. Furthermore, PO and lard have a more deleterious effect than OO on the contractile function of the aorta. Excessive consumption of saturated or unsaturated fatty acids is harmful to health, regardless of their vegetable or animal origin. As these results are not necessarily transposable to usual fat consumption, it would be interesting to study the effects of increasing doses of PO, OO, or lard to determine the adequate dose that does not compromise insulin sensitivity and aortic contractility. Further studies are needed to assess the effect of long-term consumption of OO, PO or lard in nonobesogenic diets in human.

Abbreviations

Ach:	Acetylcholine
AUC:	Area under the curve
HFD:	High-fat diet
HOMA-IR:	Homeostasis model assessment of insulin resistance
IPGTT:	Intraperitoneal glucose tolerance test
KCl:	Potassium chloride
L-NAME:	Nitro-L-arginine methyl ester
MUFA:	Monounsaturated fatty acids
NO:	Nitric oxide
OO:	Olive oil
Phe:	Phenylephrine
PO:	Palm olein
PUFA:	Polyunsaturated fatty acids
SFA:	Saturated fatty acids
SNP:	Sodium nitroprusside
SOD:	Superoxide dismutase
NS:	Not significant.

Data Availability

The data used to support the findings of this study are included within the article.

Ethical Approval

All procedures were authorized by the academic Ethics committee of the University of Montpellier (CEEA-LR-12002). The experiment was carried out in strict accordance with the guidelines for the management and use of laboratory animals. All the laboratory procedures were used to reduce the pain of the rats.

Conflicts of Interest

The authors declare that they have no conflicts of interest regarding the publication of this paper.

Authors’ Contributions

Youzan Ferdinand Djohan designed the study, carried out literature search, collected data, carried out statistical analysis, and prepared the manuscript. Fabrice Raynaud and Karen Lambert collected the data and interpreted the data. Jean-Paul Cristol designed the study and carried out literature search. Charles Coudray, Christine Feillet-Coudray, and Eric Badia designed the study, carried out literature search, collected the data, and interpreted data. Anne Virsolvy carried out literature search, collected data, interpreted data, carried out statistical analysis, and prepared the manuscript. Eric Badia and Anne Virsolvy contributed equally to this work.

Acknowledgments

This work was supported by a research grant from SANIA (no. 2015/UM/150666).

Supplementary Materials

Supplemental Figure 1: Effects of diets on total GLUT4 protein expression in muscle Representative western blot of total GLUT4 with tubulin as a normalizing gene (a). The histograms of blot quantification (b). Results were expressed as mean values ± SD, n = 7-8 animals per group. The limit of statistical significance was set at . NS: not significant. (Supplementary Materials)

References

S. Yu, X. Guo, G. Li, H. Yang, L. Zheng, and Y. Sun, “Gender discrepancy in the predictive effect of metabolic syndrome and its components on newly onset cardiovascular disease in elderly from rural China,” BMC Geriatrics, vol. 21, 2021, 505.
View at: Google Scholar
Y. Rochlani, N. V. Pothineni, S. Kovelamudi, and J. L. Mehta, “Metabolic syndrome: pathophysiology, management, and modulation by natural compounds,” Therapeutic Advances in Cardiovascular Disease, vol. 11, no. 8, pp. 215–225, 2017.
View at: Google Scholar
P. Silva Figueiredo, A. C. Inada, M. Ribeiro Fernandes et al., “An overview of novel dietary supplements and food ingredients in patients with metabolic syndrome and non-alcoholic fatty liver disease,” Molecules, vol. 23, p. 877, 2018.
View at: Publisher Site | Google Scholar
A. Aboonabi, R. R. Meyer, and I. Singh, “The association between metabolic syndrome components and the development of atherosclerosis,” Journal of Human Hypertension, vol. 33, pp. 844–855, 2019.
View at: Publisher Site | Google Scholar
J. Kaur, “A comprehensive review on metabolic syndrome,” Cardiology Research and Practice, vol. 2014, Article ID 943162, 21 pages, 2014.
View at: Publisher Site | Google Scholar
M. Ishii, A. Maeda, S. Tani, and M. Akagawa, “Palmitate induces insulin resistance in human HepG2 hepatocytes by enhancing ubiquitination and proteasomal degradation of key insulin signaling molecules,” Archives of Biochemistry and Biophysics, vol. 566, pp. 26–35, 2015.
View at: Publisher Site | Google Scholar
K. G. Jackson, K. J. Newens, M. J. Fry, A. K. Thompson, and C. M. Williams, “Differential effects of single fatty acids and fatty acid mixtures on the phosphoinositide 3-kinase/Akt/eNOS pathway in endothelial cells,” European Journal of Nutrition, vol. 61, 2022.
View at: Publisher Site | Google Scholar
M. V. Pinti, G. K. Fink, Q. A. Hathaway, A. J. Durr, A. Kunovac, and J. M. Hollander, “Mitochondrial dysfunction in type 2 diabetes mellitus: an organ-based analysis,” American Journal of Physiology. Endocrinology and Metabolism, vol. 316, no. 2, pp. E268–E285, 2019.
View at: Publisher Site | Google Scholar
M. A. Briggs, K. S. Petersen, and P. M. Kris-Etherton, “Saturated fatty acids and cardiovascular disease: replacements for saturated fat to reduce cardiovascular risk,” Healthcare (Basel, Switzerland), vol. 5, no. 2, p. 29, 2017.
View at: Google Scholar
D. D. Wang and F. B. Hu, “Dietary fat and risk of cardiovascular disease: recent controversies and advances,” Annual Review of Nutrition, vol. 37, no. 1, pp. 423–446, 2017.
View at: Publisher Site | Google Scholar
S. Hamley, “The effect of replacing saturated fat with mostly n-6 polyunsaturated fat on coronary heart disease: a meta-analysis of randomised controlled trials,” Nutrition Journal, vol. 16, p. 30, 2017.
View at: Publisher Site | Google Scholar
L. Hooper, N. Martin, O. F. Jimoh, A. Abdelhamid, and G. Davey Smith, “Reduction in saturated fat intake for cardiovascular disease,” The Cochrane Database of Systematic Reviews, vol. 8, no. 8, 2020.
View at: Google Scholar
S. Fereidoon and P. Ambigaipalan, “Omega-3 polyunsaturated fatty acids and their health benefits,” Annual Review of Food Science and Technology, vol. 9, pp. 345–381, 2018.
View at: Google Scholar
T. Morioka, K. Mori, and M. Emoto, “Is stiffness parameter β useful for the evaluation of atherosclerosis ?∼ its clinical implications, limitations, and future perspectives ∼,” Journal of Atherosclerosis and Thrombosis, vol. 28, no. 5, pp. 435–453, 2021.
View at: Publisher Site | Google Scholar
A. C. Peyter, J. B. Armengaud, E. Guillot, and C. Yzydorczyk, “Endothelial progenitor cells dysfunctions and cardiometabolic disorders: from mechanisms to therapeutic approaches,” International Journal of Molecular Sciences, vol. 22, no. 13, p. 6667, 2021.
View at: Publisher Site | Google Scholar
T. R. d. Lima, D. A. S. Silva, M. W. C. Giehl, E. D’Orsi, and D. A. González-Chica, “Clusters of cardiometabolic risk factors and their association with atherosclerosis and chronic inflammation among adults and elderly in florianópolis, Southern Brazil,” Arquivos Brasileiros de Cardiologia, vol. 117, no. 1, pp. 39–48, 2021.
View at: Publisher Site | Google Scholar
O. I. Mba, M.-J. Dumont, and M. Ngadi, “Palm oil: processing, characterization and utilization in the food industry—a review,” Food Bioscience, vol. 10, pp. 26–41, 2015.
View at: Publisher Site | Google Scholar
R. Abdul, K. Triyana, S. Sismindari, and Y. Erwanto, ““Differentiation of lard and other animal fats based on triacylglycerols composition and principal component analysis,” International Food Research Journal, vol. 19, pp. 475–479, 2012.
View at: Google Scholar
A. Montaño, M. Hernandez, I. Garrido, J. L. Llerena, and F. Espinosa, “Fatty acid and phenolic compound concentrations in eight different monovarietal virgin olive oils from extremadura and the relationship with oxidative stability,” International Journal of Molecular Sciences, vol. 17, no. 11, 2016.
View at: Google Scholar
K. Sundram, R. Sambanthamurthi, and Y. A. Tan, “Palm fruit chemistry and nutrition,” Asia Pacific Journal of Clinical Nutrition, vol. 12, pp. 355–362, 2003.
View at: Google Scholar
L. Basiricò, P. Morera, D. Dipasquale et al., “(−)-Epigallocatechin-3-gallate and hydroxytyrosol improved antioxidative and anti-inflammatory responses in bovine mammary epithelial cells,” Animal: An International Journal of Animal Bioscience, vol. 13, pp. 2847–2856, 2019.
View at: Publisher Site | Google Scholar
Y. F. Djohan, E. Badia, B. Bonafos et al., “High dietary intake of palm oils compromises glucose tolerance whereas high dietary intake of olive oil compromises liver lipid metabolism and integrity,” European Journal of Nutrition, vol. 58, no. 8, pp. 3091–3107, 2019.
View at: Publisher Site | Google Scholar
L. Metz, M. Vermaelen, K. Lambert et al., “Endurance training increases lactate transport in male Zucker fa/fa rats,” Biochemical and Biophysical Research Communications, vol. 331, no. 4, pp. 1338–1345, 2005.
View at: Publisher Site | Google Scholar
M. Elferchichi, J. Mercier, A. Bourret et al., “Is static magnetic field exposure a new model of metabolic alteration? Comparison with zucker rats,” International Journal of Radiation Biology, vol. 87, pp. 483–490, 2011.
View at: Publisher Site | Google Scholar
Y. F. Djohan, M. Camara-Cisse, G. Fouret et al., “Diets rich in olive oil, palm oil, or lard alter mitochondrial biogenesis and mitochondrial membrane composition in rat liver,” Biochemistry Research International, vol. 2022, Article ID 9394356, 10 pages, 2022.
View at: Publisher Site | Google Scholar
J. Carillon, B. Jover, J. P. Cristol, J. M. Rouanet, S. Richard, and A. Virsolvy, “Dietary supplementation with a specific melon concentrate reverses vascular dysfunction induced by cafeteria diet,” Food & Nutrition Research, vol. 60, no. 1, Article ID 32729, 2016.
View at: Publisher Site | Google Scholar
A. Fort, M. Cordaillat, C. Thollon et al., “New insights in the contribution of voltage-gated Nav channels to rat aorta contraction,” PLoS One, vol. 4, no. 10, Article ID e73602009, 2009.
View at: Publisher Site | Google Scholar
T. Jørgensen, N. Grunnet, and B. Quistorff, “One-year high fat diet affects muscle-but not brain mitochondria,” Journal of Cerebral Blood Flow and Metabolism, vol. 35, no. 6, pp. 943–950, 2015.
View at: Publisher Site | Google Scholar
P. Sangwung, K. F. Petersen, G. I. Shulman, and J. W. Knowles, “Mitochondrial dysfunction, insulin resistance, and potential genetic implications,” Endocrinology, vol. 161, no. 4, Article ID bqaa017, 2020.
View at: Publisher Site | Google Scholar
J. Carillon, C. Romain, G. Bardy et al., “Cafeteria diet induces obesity and insulin resistance associated with oxidative stress but not with inflammation: improvement by dietary supplementation with a melon superoxide dismutase,” Free Radical Biology and Medicine, vol. 65, pp. 254–261, 2013.
View at: Publisher Site | Google Scholar
C. Feillet-Coudray, M. Aoun, G. Fouret et al., “Effects of long-term administration of saturated and n-3 fatty acid-rich diets on lipid utilisation and oxidative stress in rat liver and muscle tissues,” British Journal of Nutrition, vol. 110, pp. 1789–1802, 2013.
View at: Google Scholar
M. Collino, R. Mastrocola, D. Nigro et al., “Variability in myosteatosis and insulin resistance induced by high-fat diet in mouse skeletal muscles,” BioMed Research International, vol. 2014, Article ID 569623, 10 pages, 2014.
View at: Publisher Site | Google Scholar
S. Janssens, M. M. Heemskerk, S. A. Van den Berg et al., “Effects of low-stearate palm oil and high-stearate lard high-fat diets on rat liver lipid metabolism and glucose tolerance,” Nutrition and Metabolism, vol. 12, no. 1, p. 57, 2015.
View at: Publisher Site | Google Scholar
S. H. Lee, S. Y. Park, and C. S. Choi, “Insulin resistance: from mechanisms to therapeutic strategies,” Diabetes & Metabolism Journal, vol. 46, no. 1, pp. 15–37, 2022.
View at: Publisher Site | Google Scholar
T. Sarabhai, C. Koliaki, L. Mastrototaro et al., “Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle,” Diabetologia, vol. 65, no. 2, pp. 301–314, 2022.
View at: Publisher Site | Google Scholar
H. Gill, M. Mugo, A. Whaley-Connell, C. Stump, and J. R. Sowers, “The key role of insulin resistance in the cardiometabolic syndrome,” The American Journal of the Medical Sciences, vol. 330, no. 6, pp. 290–294, 2005.
View at: Google Scholar
C. Han, Y. Liu, X. Sun et al., “Prediction of a new body shape index and body adiposity estimator for development of type 2 diabetes mellitus: the Rural Chinese Cohort Study,” British Journal of Nutrition, vol. 118, pp. 771–776, 2017.
View at: Publisher Site | Google Scholar
W. Hancock-Cerutti, J. S. Millar, S. Valentini et al., “Assessing HDL metabolism in subjects with elevated levels of HDL cholesterol and coronary artery disease,” Molecules, vol. 26, no. 22, p. 6862, 2021.
View at: Google Scholar
B. Shao, A. V. Mathew, C. Thornock et al., “Altered HDL proteome predicts incident CVD in chronic kidney disease patients,” Journal of Lipid Research, vol. 62, Article ID 100135, 2021.
View at: Publisher Site | Google Scholar
T. Karupaiah and K. Sundram, “Effects of stereospecific positioning of fatty acids in triacylglycerol structures in native and randomized fats: A review of their nutritional implications,” Nutrition & Metabolism, vol. 4, no. 1, pp. 16–32, 2007.
View at: Publisher Site | Google Scholar
M. C. O. Izar, A. M. Lottenberg, V. Z. R. Giraldez et al., “Position statement on fat consumption and cardiovascular health—2021,” Arquivos Brasileiros de Cardiologia, vol. 116, no. 1, pp. 160–212, 2021.
View at: Google Scholar
D. Kromhout, A. Menotti, A. Alberti-Fidanza et al., “Comparative ecologic relationships of saturated fat, sucrose, food groups, and A Mediterranean food pattern score to 50-year coronary heart disease mortality rates among 16 cohorts of the seven countries study,” European Journal of Clinical Nutrition, vol. 72, no. 8, pp. 1103–1110, 2018.
View at: Google Scholar
S. C. Renaud, J. C. Ruf, and D. Petithory, “The positional distribution of fatty acids in palm oil and lard influences their biologic effects in rats,” Journal of Nutrition, vol. 125, no. 2, pp. 229–237, 1995.
View at: Google Scholar
A. Alfieri, E. Imperlini, E. Nigro et al., “Effects of plant oil interesterified triacylglycerols on lipemia and human health,” International Journal of Molecular Sciences, vol. 19, no. 1, 2017.
View at: Publisher Site | Google Scholar
R. Sambanthamurthi, K. Sundram, and Y. Tan, “Chemistry and biochemistry of palm oil,” Progress in Lipid Research, vol. 39, no. 6, pp. 507–558, 2000.
View at: Publisher Site | Google Scholar
K. Raynal-Ljutovac, J. Bouvier, C. Gayet et al., “Organisation structurale et moleculaire des lipides dans les aliments: Impacts possibles sur leur digestion et leur assimilation par l’Homme,” Oilseeds and Fats, Crops and Lipids, vol. 18, no. 6, pp. 324–351, 2011.
View at: Google Scholar
C. Sanchez, V. Achard, M. Grino, and S. Tanguy, “Long term high-fat diet-induced modification of vascular wall and perivascular adipose tissue-mediated oxidative stress: consequences for endothelium-independent vascular function in rats,” International Journal of Clinical Cardiology, vol. 4, no. 2, 2017.
View at: Publisher Site | Google Scholar
E. Borello and V. Domenici, “Determination of Pigments in Virgin and Extra-Virgin Olive Oils: A comparison between two near UV-vis spectroscopic techniques,” Foods, vol. 8, no. 1, 2019.
View at: Publisher Site | Google Scholar
F. Abdullah, R. Ismail, R. Guazali, and Z. Idris, “Total phenolic contents and antioxidant activity of palm oils and palm kernel oils at various refining processes,” Journal of Oil Palm Research, vol. 30, no. 4, pp. 682–692, 2018.
View at: Google Scholar
C. Gauze-Gnagne, F. Raynaud, Y. F. Djohan et al., “Impact of diets rich in olive oil, palm oil or lard on myokine expression in rats,” Food & Function, vol. 11, pp. 9114–9128, 2020.
View at: Google Scholar
Y. Ma, G. Liu, M. Tang, J. Fang, and H. Jiang, “Epigallocatechin gallate can protect mice from acute stress induced by LPS while stabilizing gut microbes and serum metabolites levels,” Frontiers in Immunology, vol. 12, Article ID 640305, 2021.
View at: Publisher Site | Google Scholar
S. Varadharaj, O. J. Kelly, R. N. Khayat, P. S. Kumar, N. Ahmed, and J. L. Zweier, “Role of dietary antioxidants in the preservation of vascular function and the modulation of health and disease,” Frontiers in Cardiovascular Medicine, vol. 4, no. 64, 2017.
View at: Publisher Site | Google Scholar
H. Ogita and J. Liao, “Endothelial function and oxidative stress,” Endothelium: Journal of Endothelial Cell Research, vol. 11, no. 2, pp. 123–132, 2004.
View at: Publisher Site | Google Scholar
J. Joffre and J. Hellman, “Oxidative stress and endothelial dysfunction in sepsis and acute inflammation,” Antioxidants and Redox Signaling, vol. 15, pp. 1291–1307, 2021.
View at: Publisher Site | Google Scholar
O. Sorop, J. van de Wouw, S. Chandler et al., “Experimental animal models of coronary microvascular dysfunction,” Cardiovascular Research, vol. 116, no. 4, pp. 756–770, 2020.
View at: Publisher Site | Google Scholar
R. Muniyappa and J. R. Sowers, “Role of insulin resistance in endothelial dysfunction,” Reviews in Endocrine & Metabolic Disorders, vol. 14, no. 1, pp. 5–12, 2013.
View at: Publisher Site | Google Scholar
H. Cai and D. G. Harrison, “Endothelial dysfunction in cardiovascular diseases: The role of oxidant stress,” Circulation Research, vol. 87, pp. 840–844, 2000.
View at: Publisher Site | Google Scholar
A. L. Mundy, E. Haas, I. Bhattacharya et al., “Fat intake modifies vascular responsiveness and receptor expression of vasoconstrictors: Implications for diet-induced obesity,” Cardiovascular Research, vol. 73, pp. 368–375, 2007.
View at: Publisher Site | Google Scholar
S. Giannitsi, B. Maria, A. Bechlioulis, and K. Naka, “Endothelial dysfunction and heart failure: a review of the existing bibliography with emphasis on flow mediated dilation,” JRSM Cardiovascular Disease, vol. 8, Article ID 204800401984304, 2019.
View at: Publisher Site | Google Scholar
Y. F. Djohan, A. A. Monde, M. Camara-Cissé et al., “Effects of high-fat diets on inflammation and antioxidant status in rats: Comparison between palm olein and olive oil,” Acta Biochimica Polonica, vol. 68, no. 4, pp. 739–744, 2021.
View at: Google Scholar

Copyright

Copyright © 2022 Youzan Ferdinand Djohan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

1201

Downloads

1049

Citations