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International Journal of Food Science
Volume 2014, Article ID 749630, 7 pages
http://dx.doi.org/10.1155/2014/749630
Research Article

Dissolution of Lipid-Based Matrices in Simulated Gastrointestinal Solutions to Evaluate Their Potential for the Encapsulation of Bioactive Ingredients for Foods

Food Research and Development Centre, Agriculture and Agri-food Canada, 3600 Casavant O., Saint-Hyacinthe, QC, Canada J2S 8E3

Received 3 February 2014; Revised 2 May 2014; Accepted 12 May 2014; Published 12 June 2014

Academic Editor: Thierry Thomas-Danguin

Copyright © 2014 Yves Raymond and Claude P. Champagne. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of the study was to compare the dissolution of chocolate to other lipid-based matrices suitable for the microencapsulation of bioactive ingredients in simulated gastrointestinal solutions. Particles having approximately 750 μm or 2.5 mm were prepared from the following lipid-based matrices: cocoa butter, fractionated palm kernel oil (FPKO), chocolate, beeswax, carnauba wax, and paraffin. They were added to solutions designed to simulate gastric secretions (GS) or duodenum secretions (DS) at 37°C. Paraffin, carnauba wax, and bees wax did not dissolve in either the GS or DS media. Cocoa butter, FPKO, and chocolate dissolved in the DS medium. Cocoa butter, and to a lesser extent chocolate, also dissolved in the GS medium. With chocolate, dissolution was twice as fast as that with small particles (750 μm) as compared to the larger (2.5 mm) ones. With 750 μm particle sizes, 90% dissolution of chocolate beads was attained after only 60 minutes in the DS medium, while it took 120 minutes for 70% of FPKO beads to dissolve in the same conditions. The data are discussed from the perspective of controlled release in the gastrointestinal tract of encapsulated ingredients (minerals, oils, probiotic bacteria, enzymes, vitamins, and peptides) used in the development of functional foods.

1. Introduction

A variety of ingredients are used to supplement foods in order to develop functional foods in the hope of reducing the risk of diseases [1]. Popular ingredients are minerals, fibres, probiotics, antioxidants, plant sterols, and omega-3 oils. There is also interest in prebiotics, peptides, and enzymes. In some instances, the ingredient must reach the intestines in a bioactive form. In the case of probiotic bacteria or enzymes, this requires maintaining viability/activity during processing and storage and after consumption [2, 3]. With other compounds, the ingredient can generate off flavours or become oxidized. In these instances, it is advisable to encapsulate the bioactives [4] and only allow their release in the gastrointestinal tract (GIT).

Many encapsulation techniques for probiotics or other bioactives are available [5, 6]. Using lipids for encapsulation has the advantage of providing barriers to oxygen, acid, and moisture, as well as not dissolving in foods. When the goal is to deliver a bioactive ingredient which is in a powder form to the GIT, two lipid-based encapsulation techniques are found: spray-coating and spray-chilling (also termed spray-cooling) techniques. In the first, the molten lipid is sprayed at the surface of the powder [7], while in the second, the powder is blended to the molten fat and the mixture is subsequently allowed to solidify, typically in a column of cold air.

With probiotics or some enzymes, dissolution of the lipid coating is undesirable in the stomach, because of the stressful effect of the acid environment [8]. In these instances, the protective coat must not melt at temperatures below 37°C, which leaves a small number of food-grade lipids. Palm kernel oil has been suggested as a matrix for spray chilling [9] and chocolate is now recognized as a good carrier for probiotics [10]. The pharmaceutical industry also uses waxes for the controlled release of bioactives [11], but there are no comparative studies of their dissolution in the GIT environments in comparison to chocolate or oils.

The goal of this study was to evaluate the dissolution of five lipid-based compounds in simulated gastric and duodenum solutions, in order to ascertain their appropriateness as microencapsulation matrices for dried bioactives.

2. Methodology

2.1. Lipid Compounds

Six matrices were tested for their dissolution properties in simulated gastrointestinal solutions. Chocolate was the Côte d’Or 86% Cacao brand (Mondelez Intl, Halle, Belgium); the Côte d’Or label stated the following ingredient content, per 50 g of product: 27 g fat, 17 g carbohydrates (including 7 g fibres and 5 g sugar), and 5 g protein. Cocoa butter flakes were of Mycryo (Barry Callebaut) and the product was completely liquid at 37°C. It must be mentioned that chocolate, cocoa butter, and FKPO contain numerous fatty acids and that this prevents the product from having a specific melting point. The matrices subsequently have variable “solid fat content” as a function of temperature (Table 1). As data from Table 1 shows, the completely liquefied state at 37°C of the cocoa butter used in this study was typical of this product. Fractionated palm kernel oil (FPKO) was graciously provided by Barry Callebaut (Kraft Foods, Saint-Hyacinthe, QC, Canada). Unfortunately, the specific “% fat in solid state” to “temperature” properties of the FPKO product are proprietary. However, it can be stated that the completely liquefied state was reached between 42 and 44°C and that the range of temperatures that generates the solid states is much narrower than that with the cocoa butter. Beeswax USP grade and Carnauba wax were from Spectrum Chemical Manufacturing Co, (Gardena, CA, USA) and had an MP between 62–65°C and 82–85.5°C, respectively. Granular paraffin wax was from Acros Organic (New Jersey, NJ, USA) with an MP in the 50–57°C range.

tab1
Table 1: Solid fat content of cocoa butter (adapted from the data of Beckett [13]).

2.2. Gastrointestinal Solutions

Compounds used to make the gastrointestinal solutions were K2HPO4 anhydrous ACS Grade (Bioshop Canada Inc, Burlington, ON, Canada), NaHCO3 (Fluka Biochemika, Oakville, ON, Canada), and KCl (Assured BDH, Toronto, ON, Canada), while Sigma Aldrich (Oakville, ON, Canada) was the supplier of the following: NaCl, mucin from porcine stomach type III, lipase from porcine pancreas type II, pepsin from porcine gastric mucosa, alpha-amylase type VI-B from porcine pancreas, and pancreatin from porcine pancreas. Gastric secretion (GS) and duodenal secretion (DS) were prepared according to Table 2. Those composition parameters were adapted from those used in an in vitro digestive system (IViDiS) [12]. The pH values of GS and DS were of 1.5 and 6.8, respectively.

tab2
Table 2: Composition of the solutions used to prepare the simulated gastrointestinal solutions and amounts required to prepare the gastric secretion (GS) and duodenum secretion (DS) media.

2.3. Production of Lipid Particles

Two series of fat or wax particles sizes were prepared which had, on the average, 2.5 mm or 0.75 mm diameters. For the preparation of the 2.5 mm beads, the lipid matrices were melted by adding solid particles into a test tube placed in a water bath (100°C). Particles were then poured into a syringe equipped with an 18 G 11/2 (BD) needle. The molten lipids were dripped on a stainless steel surface placed on crushed ice cubes on which they solidified. With respect to the preparation of particles having 750 μm, the molten lipid in the syringe having the 18 G 11/2 needle tip was manually extruded and droplets fell into liquid nitrogen. The liquid nitrogen and fat particles were poured in a mortar and then crunched with the pestle until small granules were obtained. Once all the liquid nitrogen was evaporated, the particles were successively sieved with 1000 microns and 500 microns filters (Canadian Standard Sieve series, W.S. Tyler, St. Catharines, ON, Canada). The particles which had passed the 1000 μm mesh filter but which were retained by the 500 μm filter were kept and this product will be referred to as the “750 μm particles.” In all cases (2.5 mm and 750 μm sizes), the particles recovered were stored at 4°C until they were used.

2.4. Dissolution of Lipid-Base Matrices in Simulated Gastrointestinal Solutions

The various particles were placed into solutions that simulated gastric secretions (GS) and duodenal secretion (DS). Beads (0.8 g) were added to 7.2 mL of DS solutions or 3.08 mL of GS solutions (already at 37°C) in 15 mL conical tubes. The volume of the DS used (7.2 mL) was greater than that of the GS (3.08 mL) in order to represent the actual situation in the GIT. Indeed, as the food matrix containing the gastric ingredients passes from the stomach to the duodenum, the total volume of the digestate increases due to addition of the pancreatic juice and bile salts.

Five tubes were installed on a Cole-Parmer (Vernon Hills, IL, USA) Roto-Torque unit which carried out a gentle blending by inversion of the tubes at 55 rpm. The unit was placed in an incubator at 37°C. At given incubation times, a tube was taken and the quantity of dissolved particles, from the original 0.8 g, was estimated. Test tubes were first submitted to a vortex for 5 seconds. The suspensions were poured in a 250-micron stainless steel filter (Canadian Standard Sieve) preheated at 37°C and the filtrate was recovered.

2.5. Estimation of the Particle Dissolution Level

The level of dissolution was assessed by carrying out dry weights on the filtrate. Aluminium weighing dishes were first dried in the oven at 105°C for 30 minutes and put in a desiccator at room temperature for 20 minutes. The dry dishes were removed from the desiccator and weighed. The filtrate was then added in the dish and it was weighed again. The dishes were placed 22 h in the 105°C oven and then put in the desiccator at room temperature for 20 min and weighed. Controls of GS and DS solutions without beads were done in order to exactly ascertain the contribution of the dissolved lipid to the overweight. In some chocolate samples, the tubes were poured into the Buchner unit in which Whatman papers having 53 μm or 8 μm thresholds had been placed.

2.6. Statistical Analyses

Each dissolution treatment was repeated at least 3 times in an independent fashion (different days, fresh media, media sterilized independently, and so forth). Results are the average of these independently carried out triplicates. Statistical analyses were carried out using InStat (GraphPad, La Jolla, CA, USA) software.

3. Results

For all products, two sizes of beads were prepared in order to assess the effect of this parameter on dissolution. The 250 μm mesh filters retained the lipid particles when the volume of the bead was reduced by less than 99% or 96%, for beads originally having 2.5 mm or 750 μm, respectively (Table 3).

tab3
Table 3: Effect of filter pore size on the apparent solubilisation of chocolate (in %) in DS medium.

3.1. Cocoa Butter

The 2.5 mm cocoa butter particles dissolved in water alone within 30 minutes at 37°C (data not shown). Therefore, data were not presented in the figures on GS media and the product was not tested in the DS broth. Of the six lipid matrices tested, the cocoa butter particles were the least effective in preventing dissolution in simulated gastrointestinal conditions. Obviously, if one actually desires dissolution of the particles in the stomach, then the cocoa butter was the best of the matrices studied.

3.2. Dissolution in the Gastric Solution

Small particles (750 μm) were tested first for digestion in the GS and DS. In the GS, no dissolution was observed for beeswax, carnauba wax, and FPKO. For chocolate, a substantial dissolution was noted, which principally appeared in the first 30 minutes (Figure 1).

749630.fig.001
Figure 1: Effect of the matrix and particle size on dissolution in the simulated gastric solution (GS). Results are the average of three independent assays. Error bars represent Standard Error of the Means (SEM).

Tests were then made with the big 2.5 mm particles. It was assumed that small particles would dissolve more rapidly than the larger ones, and this was the case with chocolate, where liquefaction was 2.5 times lower with the 2.5 mm particles than that observed with the 750 μm ones (Figure 1).

Since the small particles of paraffin, beeswax, carnauba wax, and FPKO did not dissolve in the GS, the large 2.5 mm beads of these matrices were not tested in this medium.

3.3. Dissolution in the Duodenum Solution

Many studies carry out dissolution assays in the duodenum by only testing the effects of pancreatic solutions and bile salts, which are the major compounds added at the duodenum level. Although this is useful in separately assessing the effects of these duodenum-specific ingredients, this approach does not represent the actual conditions in the GIT. In this study, it was therefore decided to include the simulated saliva and gastric compounds in the DS medium (Table 2).

In the DS (Figure 2), beeswax, paraffin, and carnauba still did not dissolve, irrespective of bead size. However, the dissolution level of 750 μm chocolate particles appeared 11% higher than that in GS. Again, there was an effect of particle size in the chocolate dissolution rates, the larger beads being slower to liquefy (Figure 2).

749630.fig.002
Figure 2: Effect of the matrix on dissolution of lipid-based particles in the simulated duodenum solution (DS). Results are the average of three independent assays. Error bars represent Standard Error of the Means (SEM).

FPKO was the product that had the most different response between the two media. Extensive dissolution was reached with the 750 μm particles after 120 minutes in the DS (Figure 2), while none was noted in the GS (Figure 1). When the big beads of FPKO were placed in the DS, dissolution rate was reduced by a factor of almost 5. The lower dissolution level of the 2.5 mm FPKO particles, compared to 750 μm ones, could be explained by a 1.7x lower surface to volume ratio exposed to the dissolving elements of DS (lipases, bile salts). Particle size also affected dissolution of chocolate beads in the DS, but the effect of particle size was not as important as that for FPKO.

3.4. Effect of Filter Size on Apparent Dissolution Levels of Chocolate

The chocolate product we used had 24% fiber and protein, a sizeable fraction potentially being insoluble. Indeed, grinding of cocoa beans results in many products, one of which is called the “nonfat powder” although it does contain a small fraction of fat. Particles sizes of this cocoa “nonfat component” vary from 0.2 to 100 μm but are mostly in the 30 to 50 μm range [13]. Of all the matrices used in this study, chocolate was the only one which had ingredients other than the lipids. It was therefore examined if results might be linked to the methodology used. In light of the potential presence of cocoa powder particles having 0.2 to 100 μm sizes in the chocolate matrix, it was hypothesized that these cocoa particles could be retained in the smaller 8 μm filters but not in the 250 μm ones. Therefore, a series of chocolate dissolution analyses were carried out in DS in which a range of filters was tested (Table 3). With chocolate, the apparent dissolution values increased with filter pore size. Evidently this methodology affected data for chocolate, where nonfat cocoa particle retention by the filter occurred with the smallest pore size membranes. It can be argued that insoluble nonfat cocoa material had been released from the beads but was retained in some filters. Therefore, with chocolate, the 250 μm filters were best to evaluate chocolate melting, and data at Figures 1 and 2 were obtained with the 250 μm sieves.

4. Discussion

Encapsulation is carried out for many food ingredients in order to prevent their inactivation in the food matrix and to subsequently allow their delivery in the GIT [14]. Therefore, in some instances it can be undesirable to allow bioactive compounds to be in the soluble form in the functional food matrix during manufacture or storage of the product. In yoghurt, for example, it can be advantageous to encapsulate probiotics to limit their exposure to the acid environment [2]. Lipids are not soluble in aqueous environments. They, therefore, have potential to encapsulate bioactives for this purpose. By using spray-coating or spray-chilling technologies, it is possible to encapsulate dried hydrophilic bioactives (peptides, probiotics, and minerals) in fat matrices [15]. However, if the bioactives are added to foods for their health functions, the capsules must be able to release the hydrosoluble bioactives in the GIT.

There are various “triggers” to enable the dissolution of capsules [16]. In this study we will use melting point and GIT ingredients for this purpose. It is important to state that two GIT ingredients are not only useful for breakdown of the particle but also they allow solubilisation of the fat in the water phase. Indeed, bile salts are emulsifiers, and lipases generate free fatty acids from triglycerides which are water soluble at a pH of 7.0 when the free carboxylic end of the fatty acid chain is ionized (COO). It is on this basis that the analytical method used in this study can assess lipid particle dissolution.

The food matrix has important effects on the bioavailability and functionality of health-promoting bioactives, for both hydrophilic [17, 18] and hydrophobic bioactives [19]. It is therefore useful to examine how some encapsulation matrices will potentially deliver bioactives having various patterns of solubility.

It was known that waxes did not dissolve well in gastrointestinal media [11] and these data confirm data in the literature. Waxes can still be used in pharmaceutical applications to slow the delivery systems; in these instances, since waxes do not dissolve to rapidly release the compounds and slow release occurring by simple diffusion [11, 20], presumably, in food systems, this could be useful for the slow release of omega-3 oils and lipophilic vitamins (A, D, E, or K) in the food itself or in the GIT.

Data from this study suggest that if powders of probiotic bacteria, long chain prebiotics (e.g., inulin), peptides, enzymes, or minerals (Ca, Se) were to be coated with paraffin, beeswax, or carnauba wax, they would presumably not be released in the stomach or the duodenum. Spray-coating technologies for the encapsulation of probiotics mostly employ lipids. Chocolate, cocoa butter, and FPKO particles broke down in the gastrointestinal solutions used in this study, albeit at various rates (Figure 2). Presumably, the low melting point of the cocoa butter matrix (Table 1) resulted in its disaggregation under the 37°C experimental conditions. However, the FPKO fat did not melt at 37°C. Therefore, complete breakdown in an aqueous environment would require the action of lipases and bile salts. Evidently the integrity of the FPKO particles was not affected in the GS medium, presumably because of the absence of bile salts and because the gastric lipase was not sufficiently active in hydrolysing the FPKO triglycerides.

These data suggest that chocolate, FPKO, and cocoa butter could be used as lipid-based matrices for application in either spray-coating or spray-chilling microencapsulation technologies of bioactive ingredients in the powder form. Since chocolate, cocoa butter, and FPKO had different dissolution rates in gastrointestinal solutions, the powder components inside the lipid matrix would also, presumably, be released at variable rates in the GIT. Selection of the lipid matrix would therefore allow for controlled release in the stomach or the intestine as a function of the most desirable site. For example, with respect to most applications of probiotic bacteria where a high delivery level of viable cells in the small intestine is desirable, small particles of FPKO (750 μm) would be better than those of chocolate or cocoa butter. This is because no dissolution of FPKO occurs in the stomach, which is an environment highly detrimental to bacterial viability [2]. However, if bioactives (peptide, probiotic) would preferably be released in the stomach, for example, to fight Helicobacter pylori infections [21], then cocoa butter would be better than chocolate or FPKO. Waxes would be useless for any of these applications, because they do not dissolve the GIT environment, which would prevent the release of the bioactives in powder particles (probiotics, peptides, and minerals). However, waxes could be considered for very slow release of functional oils (omega-3 or omega-6 oils) in the duodenum. Indeed, the oils could simply diffuse outside the wax particle without requiring breakdown of the wax matrix as is done in some pharmaceutical applications [11].

Data of this study point to the importance of particle size on dissolution. With chocolate, dissolution was at least twice as fast as that in the GS and DS media with small particles (750 μm) as compared to the larger (2.5 mm) ones. The impact of bead size in dissolution of FPKO in DS is even greater. As mentioned previously, the 750 μm particles had a 1.7x higher ratio of surface to volume. Evidently, the greater the surface exposed to DS and GS solubilizing agents (bile and lipases) is, the faster the beads dissolved. These data point to the importance of characterizing particle size of microencapsulated bioactives when studying their release in the GIT. Unfortunately, this is often omitted.

Evaluating the dissolution of particles by dosing solutes in a filtrate is an easy methodology, but it has disadvantages. With chocolate, the method used to evaluate dissolution affected results. The methodology based on filtration to remove undissolved particles is best used with pure lipid compounds.

5. Concluding Remarks

Bile salts and lipases are compounds in the GIT which could allow the breakdown of fat matrices in an aqueous environment. This study’s main contribution to the field is the comparative dissolution of waxes and chocolate-based products in gastrointestinal environments.

With particular reference to probiotic bacteria, since cocoa butter dissolves very rapidly and chocolate shows partial dissolution in the stomach environment, they would potentially release the cells too early. Indeed viability loses occur in the stomach due to high acidity and enzyme [22], and means to protect the cultures need to be developed. Therefore, of the chocolate, cocoa butter, and FPKO matrices, FPKO is the one which solely dissolved in the duodenum and which would presumably release the bioactives at that level. Therefore of the various matrices tested, FPKO appears to be the most suitable to be used for the encapsulation of probiotic bacteria. Studies in dynamic in vitro systems [8, 12] are currently underway to assess if these presumptions are correct and if microencapsulation enhances the survival of probiotics to passage through the stomach.

Ethical Approval

Results from this study are all from in vitro assays. Since there were no animal or human test subjects involved in the research, there were no requirements for approval by an ethics committee. The authors have no financial relationship with the company that provided samples for encapsulation and certify that no gratification was given to them.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

Gratitude is expressed to Marylène Hétu for technical assistance. FPKO and Cocoa butter were graciously provided by Marie-Pierre Bolduc and Barry Callebaut (St. Hyacinthe QC, Canada). Thanks are also offered to Isabelle Mainville and Yves Arcand for support during the preparation of the GS and DS solutions.

References

  1. S. P. Fortmann, B. U. Burda, C. A. Senger, J. S. Lin, and E. P. Whitlock, “Vitamin and mineral supplements in the primary prevention of cardiovascular disease and cancer: an updated systematic evidence review for the U.S. preventive services task force,” Annals of Internal Medicine, vol. 159, pp. 824–834, 2013. View at Google Scholar
  2. T. Heidebach, P. Först, and U. Kulozik, “Microencapsulation of probiotic cells for food applications,” Critical Reviews in Food Science and Nutrition, vol. 52, no. 4, pp. 291–311, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. R. Karimi, A. M. Mortazavian, and A. G. Da Cruz, “Viability of probiotic microorganisms in cheese during production and storage: a review,” Dairy Science and Technology, vol. 91, no. 3, pp. 283–308, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. F. Nazzaro, P. Orlando, F. Fratianni, and R. Coppola, “Microencapsulation in food science and biotechnology,” Current Opinion in Biotechnology, vol. 23, no. 2, pp. 182–186, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Burgain, C. Gaiani, M. Linder, and J. Scher, “Encapsulation of probiotic living cells: from laboratory scale to industrial applications,” Journal of Food Engineering, vol. 104, no. 4, pp. 467–483, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. P. N. Ezhilarasi, P. Karthik, N. Chhanwal, and C. Anandharamakrishnan, “Nanoencapsulation techniques for food bioactive components: a review,” Food and Bioprocess Technology, vol. 6, no. 3, pp. 628–647, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. H. Durand and J. Panes, “Particles containing coated living micro-organisms, and method for producing same,” Unites States Patent US2003/0109025 A1, 2003. View at Google Scholar
  8. I. Mainville, Y. Arcand, and E. R. Farnworth, “A dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics,” International Journal of Food Microbiology, vol. 99, no. 3, pp. 287–296, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. D. D. L. Pedroso, M. Thomazini, R. J. B. Heinemann, and C. S. Favaro-Trindade, “Protection of Bifidobacterium lactis and Lactobacillus acidophilus by microencapsulation using spray-chilling,” International Dairy Journal, vol. 26, no. 2, pp. 127–132, 2012. View at Publisher · View at Google Scholar · View at Scopus
  10. S. Possemiers, M. Marzorati, W. Verstraete, and T. Van de Wiele, “Bacteria and chocolate: a successful combination for probiotic delivery,” International Journal of Food Microbiology, vol. 141, no. 1-2, pp. 97–103, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. B. J. Boyd, T. H. Nguyen, and A. Müllertz, “Lipids in oral controlled release drug delivery,” in Controlled Release in Oral Drug Delivery, C. G. Wilson and P. J. Crowley, Eds., pp. 299–327, Springer-Controlled Release Society, 2011. View at Google Scholar
  12. T. A. Tompkins, I. Mainville, and Y. Arcand, “The impact of meals on a probiotic during transit through a model of the human upper gastrointestinal tract,” Beneficial Microbes, vol. 2, no. 4, pp. 295–303, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. S. T. Beckett, Industrial Chocolate Manufacture and Use, Blackwell Science, Oxford, UK, 3rd edition, 1999.
  14. N. Garti, Delivery and Controlled Release of Bioactives in Foods and Nutraceuticals, Woodhead Publishing (CRC Press), Cambridge, UK, 2008.
  15. P. K. Okuro, M. Thomazini, J. C. C. Balieiro, R. D. C. O. Liberal, and C. S. Fávaro-Trindade, “Co-encapsulation of Lactobacillus acidophilus with inulin or polydextrose in solid lipid microparticles provides protection and improves stability,” Food Research International, vol. 53, no. 1, pp. 96–103, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Ubbink and J. Krüger, “Physical approaches for the delivery of active ingredients in foods,” Trends in Food Science and Technology, vol. 17, no. 5, pp. 244–254, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. M. E. Sanders and M. L. Marco, “Food formats for effective delivery of probiotics,” Annual Review of Food Science and Technology, vol. 1, no. 1, pp. 65–85, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. J. Slavin, “Fiber and prebiotics: mechanisms and health benefits,” Nutrients, vol. 5, no. 4, pp. 1417–1435, 2013. View at Publisher · View at Google Scholar · View at Scopus
  19. D. J. McClements, “Utilizing food effects to overcome challenges in delivery of lipophilic bioactives: structural design of medical and functional foods,” Expert Opinion on Drug Delivery, vol. 10, pp. 1621–1632, 2013. View at Publisher · View at Google Scholar
  20. M. U. Uhumwangho and R. S. Okor, “Effect of matrix granulation and wax coating on the dissolution rates of paracetamol granules,” African Journal of Biotechnology, vol. 5, no. 9, pp. 766–769, 2006. View at Google Scholar · View at Scopus
  21. C. M. C. Chapman, G. R. Gibson, and I. Rowland, “Health benefits of probiotics: are mixtures more effective than single strains?” European Journal of Nutrition, vol. 50, no. 1, pp. 1–17, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. B. Kos, J. Šušković, J. Goreta, and S. Matošić, “Effect of protectors on the viability of Lactobacillus acidophilus M92 in simulated gastrointestinal conditions,” Food Technology and Biotechnology, vol. 38, no. 2, pp. 121–127, 2000. View at Google Scholar · View at Scopus