Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions
Desonide is a topical corticosteroid that has been used for more than 30 years; however, its prolonged use can induce several side effects, affecting dermis and epidermis. The present work consists of development desonide-loaded nanocapsule suspensions (D-NC) using different polymers (Eudragit S100® or Eudragit L100®) and desonide-loaded lipid-core nanocapsules (D-LNC). They were formulated by interfacial deposition using the preformed polymer method and all formulations showed negative zeta potential and adequate nanotechnological characteristics (particle size 161–202 nm, polydispersity index < 0.20). Simple and sensitive methods using high-performance liquid chromatography (HPLC) were developed to quantify desonide in LNC and to study its release kinetics. The method was linear, specific, precise, and exact and therefore can be applied in quantification of D-NC and D-LNC. We evaluated in vitro methods for drug release (dissolution, Franz diffusion cells, and dialysis sac) and we use mathematical models (monoexponential, biexponential, and Korsmeyer-Peppas) to show release kinetics from this system.
Desonide is a synthetic nonfluorinated corticosteroid and has been widely used as a topical treatment of inflammation caused by a number of conditions such as allergic reactions and psoriasis for more than 30 years [1, 2]. Topical corticosteroids were introduced in medicine for 50 years and represent a landmark in dermatologic therapy, have fast action, and are the most effective anti-inflammatory drugs available, by promoting systematic improvement of clinical manifestations [3, 4]. Its clinical efficacy is mainly due to its anti-inflammatory, vasoconstrictive, proliferative, immunosuppressive action. Because of the risks of tolerance and local and systemic adverse effects, these drugs should be used in amounts, frequency, and duration of application minimum necessary for the required clinical efficacy [5–7]. The nanoencapsulation can be capable of minimizing this adverse effects; however, until now no one studied the development of desonide-loaded in nanoparticles.
In this context, the nanotechnology became an important tool on the pharmaceutical development promoting significant changes in the market of medicine production . The nanostructured carrier systems have dimensions between 10 and 1000 nm and differ according to the qualitative composition and organization at the molecular level . Loading of therapeutic agents into polymeric nanoparticles provides several benefits, including drug protection from degradation or premature metabolism, as well as sustained release and maintenance of plasma drug concentrations at therapeutic levels, besides having their toxicity reduced and/or efficacy increased [10, 11]. Studies have shown that the nanoparticles have a tendency to accumulate in inflamed tissues, providing new perspectives for action of anti-inflammatory drugs . The nanoparticles can accumulate in inflamed tissues due to the greater microvascular permeability in those sites. Thus, indomethacin-loaded nanocapsules produced an increased anti-inflammatory efficacy in rat models of inflammation (acute and chronic edema) compared with free indomethacin . Furthermore, the drugs loaded in nanocapsules are capable of the maintenance of high levels of prostaglandin over longer periods, showing good results for control of inflammatory reactions .
The polymeric nanoparticles have been described in the literature in order to reduce adverse effects and systemic absorption pertinent to corticosteroids [15–20]. For nanoparticles of type nanocapsules, the polymeric wall plays a predominant role in protecting the active substances incorporated and probably in the release profile [21, 22]; crystallinity and hydrophobic nature of polymer are key factors in determining the rate of in vitro degradation from nanoparticles . The polymers commonly used are biodegradable polyesters, especially poly--caprolactone (PCL), poly(lactide) (PLA), and poly(lactide-co-glycolide) (PLGA). Eudragit can also be used as may other polymers such as poly(alkylcyanoacrylate) (PACA) . The polymers of interest in this work, PCL [24–27], Eudragit S100 , Eudragit RS®, or Eudragit L100-55® [29, 30], have been described in the development of nanocapsules.
PCL is one polymer of the great interest in medicine due to the nontoxicity, slow degradation rate, and high permeability to many drugs and therefore has been widely used as a drug delivery vehicle [31, 32]. Eudragits are synthetic copolymers of dimethylaminoethyl methacrylates, methacrylic acid, and methacrylic acid esters in varying ratios. Eudragit L100 and Eudragit S100 are anionic polymers with chemical name based on polymeric ratios of poly(methacrylic acid, methyl methacrylate) 1 : 1 and 1 : 2, respectively. These polymers have pH-dependent solubility, Eudragit L100 dissolving at pH 6.0 and Eudragit S100 dissolving at pH 7.0 are commonly used for sustained release formulations .
Lipid-core nanocapsules (LNC), a specific type of nanoparticle which uses PCL as the polymer , have several applications such as providing sustained release of drugs ; the penetration of this nanoparticle into the skin showed retention at the outermost layers of the skin, independent of the drug penetration profile  and has been described as an intelligent way for immediate and sustained drug delivery for skin disease treatment . Moreover, LNC have shown capacity to encapsulate topical corticosteroids with mometasone furoate, suitable for prolonged treatment of skin disorders , dexamethasone, promoted increase of drug delivered into viable epidermis , betamethasone dipropionate, and dithranol, and provided decreased irritation potential of these drugs [38, 39]. LNC contains a mixture of a medium chain triglyceride (oil) and sorbitan monostearate (solid) in the core. In the case, the release of drug from lipid-core nanocapsule can be controlled varying the components of the core or polymer wall [19, 20].
In vitro drug release studies are important tool to assess the ability of the nanoparticles suspensions to control the drug release compared to solutions containing drugs not encapsulated. One of the biggest obstacles is that the in vitro drug release involves the separation of the drug still encapsulated in the particle from that drug free of particle into release medium [30, 40]. Therefore, in most techniques the monitoring of the drug release is performed by a physical separation of the nanoparticle from the release medium to discriminate the unreleased and released drug . According to Xu et al.  the methods for drug release from nanoparticles can be classified into two categories: sample and separation methods (ultrafiltration, ultracentrifugation, or centrifugal ultrafiltration techniques), like dissolution method  and membrane diffusion methods, such as dialysis sac [16, 44] and Franz diffusion cells [27, 36]. The most used technique is dialysis sac, which consists in employment of a selective membrane. The drug release in medium inside the dialysis bag to pass through the membrane is according to the laws of diffusion and the principle of dialysis .
Thus, an appropriate in vitro release study is often difficult to conduct and the results reflect complex kinetics of several simultaneous processes with hard interpretation . Mathematical models were applied to the release profiles to facilitate the interpretation of results. The monoexponential and biexponential models have been proposed to describe the drug release from nanocapsules [20, 26, 30, 43, 44]. The biexponential model is characterized by biphasic release with burst effect corresponds to initial release period, attributed either to the desorption of the drug situated on the nanocapsules surface or to the degradation of the polymeric wall surrounding the oily core containing the drug, and second phase corresponds to the diffusion of the drug molecules from oil core [23, 46].
Investigations of drug release often provide important information about the structure of the nanoparticles and the drug encapsulated physicochemical relationships for developing successful formulations . In the present work desonide-loaded nanocapsules were developed using Eudragit L100 described as copolymer 1 : 1 and Eudragit S100 copolymer 1 : 2 and, desonide-loaded lipid-core nanocapsules, in order to assess the influence of the polymeric wall on the encapsulation efficiency of desonide, measurements of size, polydispersity, zeta potential, and pH analysis. In vitro release of desonide was examined by dialysis sac; Franz diffusion cells and dissolution methods and mechanism of drug release were investigated using mathematical equations.
2. Materials and Methods
Desonide was supplied by Deg (Brazil), (purity 97,0–103,0%). Sorbitan monostearate and poly(-caprolactone) (Mw 42500) were obtained from Sigma Aldrich® (USA), Eudragit S100 and Eudragit L100 were obtained from Degussa (Darmstadt, Germany), and caprylic/capric triglyceride and polysorbate 80 were obtained from Via Farma® (Brazil). All other solvents used were of analytical or pharmaceutical grade.
2.2. Preparation of Formulations
Desonide-loaded nanocapsules (D-NC) and loaded lipid-core nanocapsules (D-LNC) were prepared using the methodology of as previously described  based on the technique from interfacial deposition of preformed polymer .
Briefly, the organic phase was composed of polymer (Eudragit S100, Eudragit L100, or Poly(-caprolactone), 0.25 g), sorbitan monostearate (0.0962 g), capric/caprylic triglyceride (400 μL), and desonide (0.0125 g) which were dissolved in acetone (62.5 mL) at 40°C. The aqueous phase containing polysorbate 80 (0.1925 g) dissolved in water (125 mL) at 40°C. Organic phase was injected into an aqueous phase and kept under magnetic stirring for 10 min. The organic solvent was eliminated and the suspension was concentrated under reduced pressure for 25 mL, adjusted in a volumetric flask. The final desonide concentration was 0.5 mg/mL.
Desonide-loaded nanocapsule suspensions using Eudragit S100 were named D-NC-S100 and using Eudragit L100 of D-NC-L100. Desonide-loaded lipid-core nanocapsules were named D-LNC and without drug of LNC.
Moreover, dispersions of the free desonide (0.5 mg/mL) were prepared with the same experimental conditions described for LNC but omitting the presence of polymer and oil, named free-desonide.
2.3. Chromatographic System
Desonide in the formulation was quantified by high performance liquid chromatography (HPLC) using a method adapted from Nguyen and coworkers . The chromatographic system was YL-Clarity® model YL9100 HPLC System, bomb model YL9110, and detector UV model YL 916, using a column Lichropher® 100 RP-18, 250 mm, 4 mm, 5 μm, Merck®. The mobile phase consisted of methanol/0.2 M acetate buffer, (60 : 40 v/v), pH adjusted to 4.0 with acetic acid, and the flow rate was 1.2 mL/min. The monitoring wavelength was 254 nm and the injection volume was 10 μL. All analyses were conducted at room temperature.
2.4. Validation Study
The objective of validation of an analytical experiment is to demonstrate that it is suitable for its intended purpose. Validation was performed following the International Conference on Harmonization (ICH) guidelines . The method was validated by its specificity, linearity, accuracy, precision, and detection and quantification limits.
Standard Solution. Standard solution was prepared at a concentration of 250 μg/mL; for this purpose 0.0125 g of desonide was accurately weighed and transferred to a volumetric flask with 50 mL of methanol.
Sample Preparation. Desonide was extracted from the LNC by the addition of 10 mL of methanol in 150 μL D-LNC (final concentration 7.5 μg/mL).
Specificity. The method specificity was evaluated through comparative analysis between solutions containing all the components of the D-LNC and LNC (except the drug).
Linearity. Linearity was verified through 3 curves in 3 consecutive days. Desonide standard solutions were prepared in methanol at 2.5; 5.0; 7.5; 10.0; 12.5; and 15.0 μg/mL.
Accuracy. To verify the method accuracy, samples were prepared by mixing the standard solution of desonide and D-LNC. Aliquots of 50, 150, and 250 μL of desonide standard solution were transferred into 10 mL volumetric flasks containing 75 μL of D-LNC. The final volume was adjusted to 10 mL with methanol in a volumetric flask, obtaining desonide concentrations of 5.0; 7.5; and 10.0 μg/mL. These studies were performed in triplicate.
Precision. Precision may be considered at two levels: repeatability (intraday precision) and interday precision. The repeatability was evaluated through the analysis of 6 sample solutions (D-LNC) at the concentration of 7.5 μg/mL in one day. Interday precision was calculated using 3 analyses of the samples at 5.0; 7.5; and 10.0 μg/mL, on 3 different days. Precision was expressed in relative standard deviation (RSD).
Detection and Quantification Limits. The limit of detection (LOD) is the lowest analyte concentration detectable, whereas the limit of quantification (LOQ) is the lower level of analyte in a sample which can be measured with suitable precision. These parameters were determined mathematically through the relationship between the standard deviation of the curve calibration and slope, accordingly proposed by the ICH . Therefore LOD and LOQ were calculated through (1), using the multiplying factor suggested by the norm of ICH, where is residual variance due to regression and is slope:
2.5. Physicochemical Characterization of Formulations
Were evaluated considering mean diameter, polydispersity index, zeta potential, pH, drug content, and encapsulation efficiencies (EE, %). For mean diameters the samples were previously diluted in ultrapure water and analyzed by dynamic light scattering (Zetasizer®, Nanoseries, UK) to determine the Z-average size and polydispersity index (PDI). Zeta potential was determined by electrophoretic mobility (Zetasizer, Nanoseries, UK) from the samples diluted in NaCl solution 10 mM (1 : 500 v/v). The pH values of the suspensions were directly measured using a potentiometer B474 (Micronal, Brazil).
The drug content in the D-LNC was assayed by (HPLC) at 254 nm, dissolving the suspensions in methanol (10 mL). The encapsulation efficiencies (EE, %) were determined by ultrafiltration-centrifugation (Microcon®, MC Millipore 10 000 Da) at 1844 ×g for 5 minutes and quantified by HPLC . The percentage of desonide encapsulated was calculated by the difference between the total (content) and free desonide concentrations (ultrafiltrate) divided by the total desonide content and multiplied by 100 .
To determine the simultaneous presence of drug crystals in the formulation, drug contents were measured in not moving sample of each formulation after 30 days. Aliquots for HPLC analyses were performed from the top of the recipient without agitating the formulation .
2.6. In Vitro Release Study
The in vitro release of free-desonide or desonide-loaded nanocapsule suspensions (D-LNC, D-NC-S100, and D-NC-L100) was evaluated using three different methods: dissolution, Franz diffusion cells, and dialysis sac. The release medium was composed of acetate buffer pH 5.5 and 1% Tween 80®, maintained under stirring in a water bath of 37.0°C. The release medium is removed at predetermined time intervals and substituted by fresh medium release to ensure the sink condition. All experiments were conducted with . The drug concentration was determined by HPLC according to the method described in the validation study and used standard curve (, ).
2.6.1. Dissolution Method
In this experiment, 1.8 mL of formulation was put in a 100 mL beaker containing 50 mL of release medium. The samples (2.0 mL) were collected and filtered through a membrane (0.1 μm, Millipore®).
2.6.2. Franz Diffusion Cells
To perform this technique vertical diffusion cell type Franz with receptor compartment with a capacity of about 6.0 mL and an area diffusion of 3.14 cm2 was used. The membranes used were cellulose acetate with a pore size of 0.45 μm Millipore, previously hydrated in ultrapure water for 24 hours before the experiment. After this time, diffusion cells were assembled keeping the membrane in contact with the release medium. The suspensions (1 mL) were added in the upper part of the membrane; two milliliters aliquots were taken out of the diffusion cells and filtered (0.45 μm Millipore filters).
Dialysis bag composed of cellulose (25 × 16 mm, cut-off 12 000 to 14 000 Da, Sigma Aldrich) was hydrated in ultrapure water for 24 hours before the experiment. 1.8 mL of the suspension was added into dialysis sac and put into closed glass flasks containing 50 mL of the release medium. Aliquots of 2 mL of release medium were collected and filtered (0.45 μm Millipore filters).
2.6.4. Particle Size
In order to verify the presence of nanoparticles in the release medium, the mean size of the particles in receptor compartment was determined by Franz diffusion cells and glass flasks by dialysis bag. The samples were withdrawing of release medium and directly measured using dynamic light scattering, at the same time intervals used for the in vitro release studies.
2.6.5. Mathematical Modeling
The release profiles were analyzed using mathematical modeling (MicroMath Scientist®). The model monoexponential (see (2)) and biexponential (see (3)) were employed to analyze the drug release profiles. The selection of the model was based on the best correlation coefficient and the best model selection criteria (MSC), both provided by the software, and the best graphic adjustment:
The observed release constants are , , and and the initial concentrations of drug are , , and .
The Korsmeyer-Peppas model (see (4)) is generally applied when the drug release mechanism is not known or when there is more than one release mechanism involved [53, 54]:where and are fraction of drug released at time and infinite time, respectively; is the release constant and is the release exponent which is indicative of the transport mechanism. The value of exponent was determined from initial 60% of drug released. For sphere geometry, indicates Fickian diffusion, indicate an anomalous behavior (non-Fickian kinetics corresponding to coupled diffusion), and indicates Case-II transport drug release (relaxational).
2.7. Statistical Analysis
All formulations were prepared and analyzed in triplicate. Results were expressed in mean ± SD (standard derivation). Analysis of the data was performed using analysis of variance (ANOVA) and Tukey post hoc test (GraphPad Software, Inc., USA). Statistical significance was considered when .
3.1. Characterization of Nanocapsule Suspensions
After preparation, all desonide-loaded nanocapsule suspensions (D-LNC, D-NC-L100, or D-NC-S100) presented a macroscopic homogeneous appearance, like a milky bluish opalescent liquid. The physicochemical characteristics of the suspensions are presented in Table 1. The mean diameters of D-NC-S100 and D-NC-L100 are similar (161–163 nm); D-LNC presented bigger diameter (202 nm). All suspensions presented negative zeta potentials (between −21.7 and −26.7 mV) and polydispersity index below 0.2, suggesting their homogeneity. The EE was dependent on the polymer present in each formulation; however the values observed were similar (between 91.0 and 98.8%). The higher values of pH were presented for D-LNC (5.28) compared to those for D-NC-L100 (4.25) and D-NC-S100 (3.90). Besides that, drug crystals were not determined in the nanocapsule suspensions after 30 days.
3.2. Validation Study
The validated method was developed for the detection and quantification of D-LNC. The mobile phase consisted of methanol/0.2 M acetate buffer in the ratio 60 : 40 v/v pH 4.0, which was considered appropriate. The desonide peak (Figure 1(a)) could be observed at retention time of 7.9 min.
Specificity was assessed by comparing desonide chromatogram with LNC chromatogram (Figure 1(b)). The latter did not present any absorption in the retention time of desonide, showing that there were no interferences of the LNC excipients in the detection of desonide.
The linearity of the method was assessed in the concentration range of 2.5–15.0 μg/mL. For each concentration, the relative standard deviation (RSD) was calculated. The calibration curve was obtained by plotting the desonide peak area versus the concentration of standard solution. The linear equation obtained was and showed adequate determination coefficient (). The validity of the assay was verified by analysis of variance. This revealed that the regression equation was linear (, ) with no linearity deviation (; ). The calculated values for LOD and LOQ were 0.73 and 2.20 μg/mL, respectively, indicating adequate sensitivity of the method for the determination the desonide.
In Table 2(a), it can be observe that six values for the repeatability were between 101.2 and 104.8% and RSD was 1.26%, demonstrating the conformity with the parameters set for the validation of chromatographic methods for drug quantification. The interday precision values are given in Table 2(b); all data are lower than the acceptance criterion of 2%.
3.3. In Vitro Drug Release Studies
The in vitro drug release from nanocapsule suspensions is one of the most studied parameters in the development of drug-loaded nanoparticles. Many in vitro methods (separation and membrane diffusion methods) have been used to evaluate the drug release from nanoparticles. With the employment dialysis sac, D-LNC and D-NC-S100 have controlled better release than D-NC-L100 and free-desonide. The D-NC-L100 presented faster release than other suspensions (Figure 2).
The characteristics about drug release profile for desonide-loaded nanocapsules and free-desonide were investigated through the mathematical modeling of the data. The model that best described the release profile for D-LNC and free-desonide was the monoexponential model, with correlation coefficients near 1 and adequate MSC values (Table 4). This model provided release constant ; this value correlates drug dissolution with time (first order kinetic). D-LNC had higher release constants ( = 0.34 h−1) than free desonide ( = 0.31 h−1); already the half-life time was higher for free-desonide (2.36 h) than D-LNC (2.06 h). On the other hand, the model that best described the release profile for D-NC-S100 and D-NC-L100 formulations was the biexponential model. This model is composed by adding two-term monoexponentials, providing two kinetic constants for release. The observed release constants for the burst phases ranged from 1.31 to 1.62 h−1 and the observed rate constant for the sustained phase from 0.03 to 0.05 h−1 for D-NC-S100 and D-NC-L100, respectively. The parameters and represent the amount of dissolved drug in the burst and sustained release phases, respectively. D-NC-S100 had higher values of desonide associated with the burst phase ( = 68% and = 31%) compared to D-NC-L100 ( = 58% and = 41%).
For the method using Franz diffusion cells (Figure 3), higher release control for D-LNC was obtained, while D-NC-S100 and D-NC-L100 maintained a similar release profile. In this case, it can be seen that the encapsulation of desonide promoted better control of release compared with free-desonide, with faster drug release. Important to note that this experiment presented the lowest concentration release of desonide among employed release methods. Moreover, the presence of nanoparticles in the receptor compartment has not been verified, showing that nonintact nanoparticle was able to overtake the barrier of the cellulose acetate membrane, which could interfere with the results of in vitro release study.
All formulations presented the same model to describe the release profile obtained from dialysis sac; D-LNC and free-desonide were the monoexponential model (Table 5). D-LNC had higher release constants ( = 0.47 h−1) than free-desonide ( = 0.40 h−1) and half-life time was higher for free-desonide (1.77 h) than D-LNC (1.51 h). The biexponential model best described the release profile for D-NC-L100 and D-NC-S100, the concentrations of desonide associated with the burst , and the sustained release phases ranged from 12 to 16% and from 83 to 87%, respectively. D-NC-S100 had higher rate constants for both burst and sustained phases ( and ) compared to D-NC-L100 and ).
In order to investigate the release mechanism of desonide-loaded nanocapsule suspensions, the resultant data were fitted to the Korsmeyer-Peppas model. The , MSC, and values are presented in Table 6. The accuracy of fit was evaluated by calculating correlation coefficient ; a satisfactory fit of the experimental data to the model was observed, through correlation coefficient near 1. The values obtained from Korsmeyer-Peppas model obtained from dialysis sac method indicated diffusion controlled Fickian release for D-NC-L100 and D-NC-L100; already D-LNC showed non-Fickian (anomalous) release. On the other hand, the results obtained from Franz diffusion cells method indicated diffusion controlled Fickian release for all formulations.
In order to investigate what happens to the particle in drug release, studies used photon correlation spectroscopy (PCS) analysis to compare the particle size distribution profiles in the receptor medium over the time of drug release profile (Figure 4).
The results from dialysis sac method showed that the particle size of the D-NC-S100 and D-NC-L100 decreased over time, indicating weight loss of the polymer, suggesting an erosion process in the release of the drug. D-NC-S100, the process of release of desonide by erosion of the polymer chain, begins with ten minutes of the experiment and remains until the end (72 h), demonstrating that the erosion process starts before the first hour in the release of the drug. Already, for the D-NC-L100 the erosion process starts in the first hour and continues until tenth hour, showing drug release by diffusion followed erosion process after first hour. Moreover, after tenth hour it was not possible to experimentally determine the mean size, indicating the solubilization of polymer in release medium.
The dissolution method (Figure 5) was applied for not using membrane. Total release of desonide was observed in five hours; D-LNC obtained slower release than other formulations in this time (near 80%).
Due to absence of membrane in the dissolution method, the drug is rapidly released from the nanoparticles, not being possible to study the kinetics of drug release using mathematical models.
Desonide has no inclusion in any pharmacopoeia ; however the determination of desonide in biological samples had already been performed by HPLC [56, 57], as well as ear drops , lotion hair [55, 59], creams, and ointments [49, 60], but never was encapsulated or quantified in nanoparticles. These colloidal drug carrier systems such as nanocapsules are analytical challenge due to the variety of compounds in the formulation and also the need to detect small amounts of drugs . Therefore the importance of the results obtained in this work is due to not only the quantification of desonide but also mostly the quantification of this drug in a colloidal vehicle.
Comparing the quantification of D-LNC with ointment  is possible to realize that using the same mobile phase the peak of desonide came out in 7.9 min later obtained in the ointment which was around 4.0 min. However, ointment which indicates the presence of two peaks degradation products of desonide in times 1.0 and 2.0 min; probably the lipid-core nanocapsules provided protection from degradation. Santa et al.  method was developed to quantify desonide in hair lotion using the different mobile phase but showed the peak of drug in the time similar to ours (around 8.5 minutes).
Desonide-loaded nanocapsule suspensions used in this study prepared with different polymers had mean diameter and polydispersity index values in accordance with that expected for these systems [16, 20]. The type and concentration of the polymer are crucial to the particle size [11, 63]. The nanocapsules obtained from the PCL polymer showed larger particle diameter than the nanoparticles with Eudragit polymers, according to result as reported by Leroux et al. . The zeta potential values for nanocapsules are a consequence of the particle coating with presence of nonionic species with long hydrophilic chain, such as polysorbate 80 acting as a physical stabilizer at the particle/water interface . These formulations were sterically stabilized by the polysorbate layer around the particles, guaranteed by the interaction of hydrophilic chains [26, 65]. All formulations showed acid pH values, in agreement with previous studies [44, 66, 67]. The formulations presented encapsulation efficiency (E.E) higher than 90% (values between 91.0 and 98.8%). According to Mora-Huertas et al. , various parameters with drug, polymer, oil, and nanocapsule preparation method are determinant factors of drug encapsulation efficiency. Besides that, no nanocrystal was formed in formulations, indicating that the drug is adsorbed on the nanocapsules, but the system is not oversaturated and the formulation is stable .
Drug release from nanoparticles depends on diffusion, particle erosion, or the combination of these . The maintaining the sink condition is a crucial requirement for in vitro drug release studies in the pharmaceutical field; this condition determines that drug concentration in the dissolution or release medium may not exceed 10 to 33% of drug solubility in these mediums . Due to the very low aqueous solubility drugs, these special techniques to study the release from nanoparticles under sink conditions too need very sensitive methods for drug quantification .
Incomplete release of desonide from nanocapsules could be attributed to the retention capacity of the polymer, as described with indomethacin-loaded nanocapsules , primidone-loaded nanocapsules , and atovaquone-loaded nanocapsules with different polymers . However, desonide presented 90% release from dissolution method after 2 h for all the formulations tested, demonstrating a limitation of technique previously tested. Result of agreement with Marchais et al.  showed that the release of phenylbutazone-loaded nanocapsules from different polyesters is affected by the type of polymer and the experimental conditions. Dissolution study was already described for determination of release from the nanoparticles by Zili et al. .
In relation to methodologies using membranes (dialysis and Franz) which are more used with nanocapsules, different results were found that can be attributed to experimental conditions. Moreover, de Andrade et al.  demonstrated the importance of the choice of the synthetic membrane using the Franz diffusion cells technique. The dialysis membrane (12 kDa pore size) was suggested as the best choice for this kind of experiment, in comparison with polycarbonate (0.05 μm) or cellulose acetate (0.45 μm) membranes, because it has a limiting barrier for nanocapsules. In addition, control release presented by free desonide using dialysis sac method can be explained due to the use sorbitan monostearate in preparation. Yoshioka et al.  showed that release from vesicles depended on the surfactant used in the preparation. Furthermore, it was demonstrated that increase in the sorbitan monostearate concentration increased the resistance to the drug diffusion in nanoparticle .
In all methodologies, D-NC-L100 resulted in the faster release of desonide between nanocapsule suspensions, which can be attributed to pH-dependent solubility of polymer Eudragit L100 . This polymer is soluble in pH 6 and release medium used pH 5.5, therefore near the pH value that it is soluble.
In all methodologies, D-NC-L100 with diameter lower particle resulted in the faster release of desonide between nanocapsule suspensions, as described by Zili et al.  who showed that nanoparticle size can influence the nanocapsule dissolution rate which increases as particle size decreases, due to an increase of available surface area.
The models that described the release profile were equal regardless of the release method used. D-LNC and free-desonide were monoexponential model and, D-NC-L100 and D-NC-S100 were biexponential model. This result is in agreement with previous research, where drug release from nanocapsules was according to a monoexponential or biexponential model [20, 26, 30].
Drug release kinetics of desonide-loaded nanocapsule suspensions were analyzed using Korsmeyer-Peppas model and showing Fickian diffusion for all suspensions obtained by Franz diffusion cells method. However, the study for determining the particle diameter over the time of drug release showed a reduction in particle diameter for D-NC-S100 and D-NC-L100 indicating occurrence of erosion process in the desonide release. Dialysis sac method showed non-Fickian (anomalous) release for D-LNC, in which the mass transfer occurs due to the drug Fickian diffusion and the relaxation of the polymer chains . However, in this case no change in the particle diameter was observed.
Desonide-loaded nanocapsule suspensions prepared with PCL, Eudragit L100, or Eudragit S100 showed good physicochemical properties. The analytical method for the quantification of D-LNC was validated in the range of 2.5 to 15.0 μg/mL. The method specificity, linearity, precision, and accuracy were validated and considered adequate. The type of the polymer and in vitro methods for desonide release influenced the result obtained and in vitro drug-model release from formulations showed monoexponential (D-LNC and free desonide) and biexponential (D-NC-L100 and D-NC-S100) release profile, regardless of the type of drug release method. Therefore, we demonstrated that Korsmeyer-Peppas model did not succeed to determine the mechanism of desonide release from nanocapsule suspensions.
The authors declare no conflict of interests regarding this article content.
M. B. Antonow thanks CAPES-Brazil for his Master Fellowship. The authors thank the financial support of CAPES, CNPq, and FAPERGS.
L. H. Kircik, “Treatment of scalp and facial seborrheic dermatitis with desonide hydrogel 0.05%,” Journal of Clinical and Aesthetic Dermatology, vol. 2, no. 2, pp. 32–36, 2009.View at: Google Scholar
B. Elewski, “An investigator-blind, randomized, 4-week, parallel-group, multicenter pilot study to compare the safety and efficacy of a nonsteroidal cream (Promiseb Topical Cream) and desonide cream 0.05% in the twice-daily treatment of mild to moderate seborrheic dermatitis of the face,” Clinics in Dermatology, vol. 27, no. 6, pp. S48–S53, 2009.View at: Publisher Site | Google Scholar
N. Rocha, M. Horta, and M. Selores, “Terapêutica tópica em dermatologia pediátrica,” Revista Nascer e Crescer, vol. 13, no. 3, pp. 215–225, 2004.View at: Google Scholar
A. D. Costa, S. Machado, and M. Selores, “Corticóides tópicos considerações sobre a sua aplicação na patologia cutânea,” Revista Portuguesa de Medicina Geral e Familiar, vol. 21, pp. 367–373, 2005.View at: Google Scholar
P. S. Grau, “Corticoides tópicos. Actualización,” Medicina Cutánea Ibero-Latino-Americana, vol. 34, no. 1, pp. 33–38, 2006.View at: Google Scholar
M. C. Fontana, K. Coradini, A. R. Pohlmann, S. S. Guterres, and R. C. R. Beck, “Nanocapsules prepared from amorphous polyesters: effect on the physicochemical characteristics, drug release, and photostability,” Journal of Nanoscience and Nanotechnology, vol. 10, no. 5, pp. 3091–3099, 2010.View at: Publisher Site | Google Scholar
J. Kreuter, Colloidal Drug Delivery Systems, Marcel Dekker, New York, NY, USA, 1994.
M. C. Fontana, K. Coradini, S. S. Guterres, A. R. Pohlmann, and R. C. R. Beck, “Nanoencapsulation as a way to control the release and to increase the photostability of clobetasol propionate: influence of the nanostructured system,” Journal of Biomedical Nanotechnology, vol. 5, no. 3, pp. 254–263, 2009.View at: Publisher Site | Google Scholar
A. R. Pohlmann, V. Weiss, O. Mertins, N. Pesce da Silveira, and S. Stanisçuaski Guterres, “Spray-dried indomethacin-loaded polyester nanocapsules and nanospheres: development, stability evaluation and nanostructure models,” European Journal of Pharmaceutical Sciences, vol. 16, no. 4-5, pp. 305–312, 2002.View at: Publisher Site | Google Scholar
R. B. Friedrich, B. Kann, K. Coradini, H. L. Offerhaus, R. C. R. Beck, and M. Windbergs, “Skin penetration behavior of lipid-core nanocapsules for simultaneous delivery of resveratrol and curcumin,” European Journal of Pharmaceutical Sciences, vol. 78, pp. 204–213, 2015.View at: Publisher Site | Google Scholar
S. R. Schaffazick, I. R. Siqueira, A. S. Badejo et al., “Incorporation in polymeric nanocapsules improves the antioxidant effect of melatonin against lipid peroxidation in mice brain and liver,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 69, no. 1, pp. 64–71, 2008.View at: Publisher Site | Google Scholar
A. Melero, A. F. Ourique, S. S. Guterres et al., “Nanoencapsulation in lipid-core nanocapsules controls mometasone furoate skin permeability rate and its penetration to the deeper skin layers,” Skin Pharmacology and Physiology, vol. 27, no. 4, pp. 217–228, 2014.View at: Publisher Site | Google Scholar
T. C. Beber, D. F. de Andrade, P. dos Santos Chaves, A. R. Pohlmann, S. S. Guterres, and R. C. Beck, “Cationic polymeric nanocapsules as a strategy to target dexamethasone to viable epidermis: skin penetration and permeation studies,” Journal of Nanoscience and Nanotechnology, vol. 16, no. 2, pp. 1331–1338, 2016.View at: Publisher Site | Google Scholar
J. Weber, N. L. Funk, M. H. Motta et al., “Association of borage oil and betamethasone dipropionate in lipid-core nanocapsules: characterization, photostability and in vitro irritation test,” Journal of Nanoscience and Nanotechnology, vol. 16, no. 2, pp. 1354–1362, 2016.View at: Publisher Site | Google Scholar
D. F. de Andrade, C. Zuglianello, A. R. Pohlmann, S. S. Guterres, and R. C. R. Beck, “Assessing the in vitro drug release from lipid-core nanocapsules: a new strategy combining dialysis sac and a continuous-flow system,” AAPS PharmSciTech, vol. 16, no. 6, pp. 1409–1417, 2015.View at: Publisher Site | Google Scholar
A. R. da Silva, E. D. Zaniquelli, M. O. Baratti et al., “Drug release from microspheres and nanospheres of poly(lactide-co-glycolide) without sphere separation from release médium,” Journal Of The Brazilian Chemical Society, vol. 21, no. 2, pp. 214–225, 2010.View at: Google Scholar
R. C. R. Beck, A. R. Pohlmann, C. Hoffmeister et al., “Dexamethasone-loaded nanoparticle-coated microparticles: correlation between in vitro drug release and drug transport across Caco-2 cell monolayers,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 67, no. 1, pp. 18–30, 2007.View at: Publisher Site | Google Scholar
H. Marchais, S. Benali, J. M. Irache, C. Tharasse-Bloch, O. Lafont, and A. M. Orecchioni, “Entrapment efficiency and initial release of phenylbutazone from nanocapsules prepared from different polyesters,” Drug Development and Industrial Pharmacy, vol. 24, no. 9, pp. 883–888, 1998.View at: Publisher Site | Google Scholar
S. S. Guterres, M. P. Alves, and A. R. Pohlmann, “Polymeric nanoparticles, nanospheres and nanocapsules, for cutaneous applications,” Drug Target Insights, vol. 2, pp. 147–157, 2007.View at: Google Scholar
H. Fessi, F. Puisieux, and J. P. de Vissaguet, “Procédé de préparation de systèmes colloidaux dispersibles d'une substance, sous forme de nanocapsules,” European Patent 0274961 A1, 1988View at: Google Scholar
International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text and Methodology Q2(R1), 2005
A. R. Pohlmann, G. Mezzalira, C. D. G. Venturini et al., “Determining the simultaneous presence of drug nanocrystals in drug-loaded polymeric nanocapsule aqueous suspensions: a relation between light scattering and drug content,” International Journal of Pharmaceutics, vol. 359, no. 1-2, pp. 288–293, 2008.View at: Publisher Site | Google Scholar
A. Vonaparti, E. Lyris, I. Panderi, M. Koupparis, and C. Georgakopoulos, “Direct injection LC/ESI-MS horse urine analysis for the quantification and identification of threshold substances for doping control. I. Determination of hydrocortisone,” Journal of Mass Spectrometry, vol. 43, no. 9, pp. 1255–1264, 2008.View at: Publisher Site | Google Scholar
V. D. Gupta, “Chemical stability of desonide in ear drops,” International Journal of Pharmaceutical Compounding, vol. 11, no. 1, pp. 79–81, 2007.View at: Google Scholar
A. M. di Pietra, V. Andrisano, R. Gotti, and V. Cavrini, “On-line post-column photochemical derivatization in liquid chromatographic—diode-array detection analysis of binary drug mixtures,” Journal of Pharmaceutical and Biomedical Analysis, vol. 14, no. 8–10, pp. 1191–1199, 1996.View at: Publisher Site | Google Scholar
T. T. Nguyen, R. Kringstad, A. J. Aasen, and K. E. Rasmussen, “Identification and analysis of a degradation product of the glucocorticoid desonide in ointment,” Acta Chemica Scandinavica, Series B: Organic Chemistry and Biochemistry, vol. 42, no. 6, pp. 403–407, 1988.View at: Google Scholar
D. Quintanar-Guerrero, E. Allémann, H. Fessi et al., “Preparation and characterization of nanocapsules from preformed polymers by a new process based on emulsification-diffusion technique,” Drug Development and Industrial Pharmacy, vol. 24, pp. 1113–1128, 1998.View at: Google Scholar
J.-C. Leroux, E. Allémann, E. Doelker, and R. Gurny, “New approach for the preparation of nanoparticles by an emulsification-diffusion method,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 41, no. 1, pp. 14–18, 1995.View at: Google Scholar
L. A. Fiel, M. D. Adorne, S. S. Guterres, P. A. Netz, and A. R. Pohlmann, “Variable temperature multiple light scattering analysis to determine the enthalpic term of a reversible agglomeration in submicrometric colloidal formulations: a quick quantitative comparison of the relative physical stability,” Colloids and Surfaces A, vol. 431, pp. 93–104, 2013.View at: Publisher Site | Google Scholar
R. T. Branquinho, V. C. F. Mosqueira, E. K. Kano et al., “HPLC-DAD and UV-spectrophotometry for the determination of lychnopholide in nanocapsule dosage form: validation and application to release kinetic study,” Journal of Chromatographic Science, vol. 52, no. 1, pp. 19–26, 2014.View at: Publisher Site | Google Scholar